BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.2241.p (450 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003775254.1| amidase family protein [Herbaspirillum serop... 707 0.0 gb|EFV83570.1| amidase [Achromobacter xylosoxidans C54] 523 e-146 ref|YP_003280193.1| amidase [Comamonas testosteroni CNB-2] >gi|2... 520 e-145 ref|YP_001562473.1| amidase [Delftia acidovorans SPH-1] >gi|1603... 520 e-145 ref|YP_004490413.1| Amidase [Delftia sp. Cs1-4] >gi|333746881|gb... 520 e-145 >ref|YP_003775254.1| amidase family protein [Herbaspirillum seropedicae SmR1] gb|ADJ63346.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 450 Score = 707 bits (1825), Expect = 0.0, Method: Composition-based stats. Identities = 414/450 (92%), Positives = 435/450 (96%) Query: 1 MPSTLSQLAADLDAGRITSLQLTEQALARIADPAGEGARVFTSVYADQARAAARASDTLR 60 MPSTLS+LAADL AG TSLQLTEQALARIADPAGEGARVFT VYA+QARAAARASDTLR Sbjct: 1 MPSTLSRLAADLAAGHTTSLQLTEQALARIADPAGEGARVFTRVYAEQARAAARASDTLR 60 Query: 61 AAKLCRSPLEGVPVSVKDLFDVAGETTLAGSVVLRGKPAATAHAAVVQNLMKAGAIVIGK 120 AA+LCRSPLEGVPVSVKDLFDVAGETTLAGSVVLRGKP A+AHAAVVQNL+KAGAI+IGK Sbjct: 61 AARLCRSPLEGVPVSVKDLFDVAGETTLAGSVVLRGKPVASAHAAVVQNLLKAGAILIGK 120 Query: 121 TNMTEFAYSGLGINPHYGTPQNTWERNVEGGRIPGGSSSGAAISVTDGMAFAAVGSDTGG 180 TNMTEFAYSGLGINPHYGTPQN WERN+EGGRIPGGSSSGAAISVTDGMAFAAVGSDTGG Sbjct: 121 TNMTEFAYSGLGINPHYGTPQNAWERNIEGGRIPGGSSSGAAISVTDGMAFAAVGSDTGG 180 Query: 181 SVRIPSALNGLTGFKPTASRVSMQGVLPLSAYLDSIGPLAVSVECCAIMDAVLAGEAYVS 240 SVRIPSALNGLTGFKPTA+RVSMQGVLPLSAYLDSIGPLAVSV+CCAIMDAVLAGE YV+ Sbjct: 181 SVRIPSALNGLTGFKPTAARVSMQGVLPLSAYLDSIGPLAVSVQCCAIMDAVLAGEDYVA 240 Query: 241 VVAHPLRGLRLLAPTNVVLDAMDETVGKAYRAALDKLRAAGAVIVEAEVPAFNALAAINA 300 V+AHPLRGLRLLAPTNVVLD MD+TVG+AYR AL KL AAGA+IVEAEVPAFNALAAINA Sbjct: 241 VLAHPLRGLRLLAPTNVVLDGMDDTVGQAYRTALGKLAAAGAMIVEAEVPAFNALAAINA 300 Query: 301 KGGFTAAEAYAWHRALIAENAAGYDQRVVSRILRGKDMSAADLLDVLHARPRWIAEVQAQ 360 KGGFTAAEAYAWHRALIAENAAGYDQRVVSRILRGKDMSAADLLDVLHARPRWIA+V+AQ Sbjct: 301 KGGFTAAEAYAWHRALIAENAAGYDQRVVSRILRGKDMSAADLLDVLHARPRWIAQVEAQ 360 Query: 361 LAGYDALVMPTVPIVAPRIAELQASDDAYYAANGKILRNPTLINFLDGCAVSLPCHAPGT 420 LAGYDALVMPTVPIVAPRIAELQASDDAYY ANGK+LRNPTLINFLDGCA+SLPCHAPG+ Sbjct: 361 LAGYDALVMPTVPIVAPRIAELQASDDAYYGANGKMLRNPTLINFLDGCALSLPCHAPGS 420 Query: 421 APVGLSLAAAGGEDKRLLAIALAVEGLLAA 450 APVGLSLAA GG+DKRLL+I LAVE LLAA Sbjct: 421 APVGLSLAACGGQDKRLLSIGLAVEALLAA 450 >gb|EFV83570.1| amidase [Achromobacter xylosoxidans C54] Length = 448 Score = 523 bits (1348), Expect = e-146, Method: Composition-based stats. Identities = 306/450 (68%), Positives = 357/450 (79%), Gaps = 2/450 (0%) Query: 1 MPSTLSQLAADLDAGRITSLQLTEQALARIADPAGEGARVFTSVYADQARAAARASDTLR 60 M TL+ L A L+ GR TS++LTE ALAR DP GEGAR FT +YA+ A A ARASDTLR Sbjct: 1 MYPTLNDLTAALNDGRSTSVELTEAALARAGDPDGEGARAFTRLYAESAMAQARASDTLR 60 Query: 61 AAKLCRSPLEGVPVSVKDLFDVAGETTLAGSVVLRGKPAATAHAAVVQNLMKAGAIVIGK 120 AA + RS +EG+P+S+KDLFDV GETT+AGSV G+PAA A+A VVQ L+ AGA++IG+ Sbjct: 61 AAGIVRSAIEGLPISIKDLFDVEGETTMAGSVAREGEPAADANAEVVQRLIAAGAVIIGR 120 Query: 121 TNMTEFAYSGLGINPHYGTPQNTWERNVEGGRIPGGSSSGAAISVTDGMAFAAVGSDTGG 180 TNMTEFAYSGLGINPHYGTP N W+R GRIPGGSSSGAA+SV DGMA A +GSDTGG Sbjct: 121 TNMTEFAYSGLGINPHYGTPLNPWDRAT--GRIPGGSSSGAAVSVADGMAVAGIGSDTGG 178 Query: 181 SVRIPSALNGLTGFKPTASRVSMQGVLPLSAYLDSIGPLAVSVECCAIMDAVLAGEAYVS 240 SVRIP+AL GLTGFKP+A RVSM+GVLPLSA LDSIGP+A SV CCA +DA+L+GE Sbjct: 179 SVRIPAALCGLTGFKPSAWRVSMEGVLPLSANLDSIGPIAASVRCCAELDAILSGEGGPV 238 Query: 241 VVAHPLRGLRLLAPTNVVLDAMDETVGKAYRAALDKLRAAGAVIVEAEVPAFNALAAINA 300 A PLRGLRL P + LD MD+ V + A+ +L+ AGA++ E +P F LA INA Sbjct: 239 PEALPLRGLRLAVPRTLALDGMDKHVSDTFARAVARLKEAGALVDEIAIPEFAELANINA 298 Query: 301 KGGFTAAEAYAWHRALIAENAAGYDQRVVSRILRGKDMSAADLLDVLHARPRWIAEVQAQ 360 KGGFTAAEA+AWHR LIA YD RVVSRILRGKDMSAAD LD+L AR W+A V + Sbjct: 299 KGGFTAAEAWAWHRELIARAGKRYDPRVVSRILRGKDMSAADYLDLLDAREAWVAAVDRR 358 Query: 361 LAGYDALVMPTVPIVAPRIAELQASDDAYYAANGKILRNPTLINFLDGCAVSLPCHAPGT 420 +AGYDAL+MPT PIVAP +A+LQASD+AYYAANG ILRNPTLINFLDGCA+SLPCHA GT Sbjct: 359 IAGYDALIMPTTPIVAPAVADLQASDEAYYAANGLILRNPTLINFLDGCALSLPCHAAGT 418 Query: 421 APVGLSLAAAGGEDKRLLAIALAVEGLLAA 450 APVGL +AA+ G D+R+LAI LAVE LLAA Sbjct: 419 APVGLMIAASNGADRRVLAIGLAVEELLAA 448 >ref|YP_003280193.1| amidase [Comamonas testosteroni CNB-2] gb|ACY34897.1| Amidase [Comamonas testosteroni CNB-2] Length = 454 Score = 520 bits (1340), Expect = e-145, Method: Composition-based stats. Identities = 301/447 (67%), Positives = 356/447 (79%), Gaps = 4/447 (0%) Query: 5 LSQLAADLDAGRITSLQLTEQALARIADPAGEGARVFTSVYADQARAAARASDTLRAAKL 64 L Q++ L G+ +++QLTEQAL A GEG RVFT + +QA A ARASD LRAA L Sbjct: 8 LDQVSERLAQGQTSAVQLTEQALEHAAQ--GEGPRVFTRLLREQALAEARASDALRAAGL 65 Query: 65 CRSPLEGVPVSVKDLFDVAGETTLAGSVVLRGKPAATAHAAVVQNLMKAGAIVIGKTNMT 124 RSPLEG+P+SVKDLFD+AG TL GS +L AT A VVQ L +AGA+++G TNMT Sbjct: 66 ARSPLEGLPISVKDLFDIAGHPTLGGSRLLADAAPATQTAEVVQRLRRAGAVIVGTTNMT 125 Query: 125 EFAYSGLGINPHYGTPQNTWERNVEGGRIPGGSSSGAAISVTDGMAFAAVGSDTGGSVRI 184 EFAYSGLG+NPHYGTP+N W+R+++GGRIPGGSSSGAAISV+DGMA AA+GSDTGGSVRI Sbjct: 126 EFAYSGLGLNPHYGTPRNPWQRDIDGGRIPGGSSSGAAISVSDGMALAAIGSDTGGSVRI 185 Query: 185 PSALNGLTGFKPTASRVSMQGVLPLSAYLDSIGPLAVSVECCAIMDAVLAGEAYVSVVAH 244 PSAL GLTGFKPTA RVSMQGVLPLSA LDSIGPLA SV CCA +DAVL+GE + + A Sbjct: 186 PSALCGLTGFKPTARRVSMQGVLPLSANLDSIGPLAPSVRCCAALDAVLSGEPHGELRAA 245 Query: 245 PLRGLRLLAPTNVVLDAMDETVGKAYRAALDKLRAAGAVIVEAEVPAFNALAAINAKGGF 304 L+GLRLL PTNVV+D MD V A++ AL L AAG I EA VP F+ LA INAKGGF Sbjct: 246 SLQGLRLLVPTNVVMDGMDSAVAAAWQHALSLLYAAGVQITEAVVPPFSELAQINAKGGF 305 Query: 305 TAAEAYAWHRALI--AENAAGYDQRVVSRILRGKDMSAADLLDVLHARPRWIAEVQAQLA 362 TAAEA+AWHR+ I A A YD RV +RILRGKD+SAAD +++L R +WIA VQAQ+A Sbjct: 306 TAAEAWAWHRSHIDGAGRLAEYDPRVGTRILRGKDISAADYIELLARRQQWIARVQAQMA 365 Query: 363 GYDALVMPTVPIVAPRIAELQASDDAYYAANGKILRNPTLINFLDGCAVSLPCHAPGTAP 422 +D ++MPTVP++AP+IAELQASD+AY+AANG +LRNPTLINFLDGCAVSLPCH G AP Sbjct: 366 DHDLMLMPTVPVIAPKIAELQASDEAYFAANGLMLRNPTLINFLDGCAVSLPCHRAGEAP 425 Query: 423 VGLSLAAAGGEDKRLLAIALAVEGLLA 449 VGLSLA G+D+RLL++ALAVE LLA Sbjct: 426 VGLSLAGLAGQDQRLLSVALAVEALLA 452 >ref|YP_001562473.1| amidase [Delftia acidovorans SPH-1] gb|ABX34088.1| Amidase [Delftia acidovorans SPH-1] Length = 456 Score = 520 bits (1340), Expect = e-145, Method: Composition-based stats. Identities = 300/447 (67%), Positives = 347/447 (77%), Gaps = 2/447 (0%) Query: 4 TLSQLAADLDAGRITSLQLTEQALARIADPAGEGARVFTSVYADQARAAARASDTLRAAK 63 +L + L G TS+QLTEQALAR +GEG +VFT V+ A A ARASD LRAA Sbjct: 9 SLELASQRLHGGETTSVQLTEQALARTM--SGEGPKVFTRVFQGAALAEARASDVLRAAG 66 Query: 64 LCRSPLEGVPVSVKDLFDVAGETTLAGSVVLRGKPAATAHAAVVQNLMKAGAIVIGKTNM 123 L RSP+EG+PVSVKDLFD+AG TL GS +L P A A VVQ L +AGA+++G TNM Sbjct: 67 LARSPIEGLPVSVKDLFDIAGFPTLGGSRLLADAPPAQRTAEVVQRLRQAGAVIVGTTNM 126 Query: 124 TEFAYSGLGINPHYGTPQNTWERNVEGGRIPGGSSSGAAISVTDGMAFAAVGSDTGGSVR 183 TEFAYSGLGINPHYGTP+N W+R+ GGRIPGGSSSGAAISVTDGMA AA+GSDTGGSVR Sbjct: 127 TEFAYSGLGINPHYGTPRNPWQRDEGGGRIPGGSSSGAAISVTDGMAMAAIGSDTGGSVR 186 Query: 184 IPSALNGLTGFKPTASRVSMQGVLPLSAYLDSIGPLAVSVECCAIMDAVLAGEAYVSVVA 243 IPSAL GLTGFKPTA RVSM+GVLPLSA LDSIGPLA SV CCA +DA+L+GE + A Sbjct: 187 IPSALCGLTGFKPTARRVSMEGVLPLSANLDSIGPLAPSVRCCATLDAILSGEPLGELHA 246 Query: 244 HPLRGLRLLAPTNVVLDAMDETVGKAYRAALDKLRAAGAVIVEAEVPAFNALAAINAKGG 303 PL+GLRLLAPTNVVLD MD TV A+ AL L AGA I V F LA IN+KGG Sbjct: 247 APLQGLRLLAPTNVVLDGMDATVAAAWERALSLLSQAGAHITHVVVAPFGELADINSKGG 306 Query: 304 FTAAEAYAWHRALIAENAAGYDQRVVSRILRGKDMSAADLLDVLHARPRWIAEVQAQLAG 363 FTAAEA+AWHR IA + YD RV +RILRGKD+SAAD +D+L R +WIA V AQ+A Sbjct: 307 FTAAEAWAWHRHHIATRLSEYDPRVGTRILRGKDISAADYIDLLARRRQWIAAVSAQMAD 366 Query: 364 YDALVMPTVPIVAPRIAELQASDDAYYAANGKILRNPTLINFLDGCAVSLPCHAPGTAPV 423 +D +VMPTVP+VAP+IA+L SD+AY+ ANG ILRNPTLINFLDGCA+SLPCH G APV Sbjct: 367 HDLIVMPTVPVVAPKIADLTQSDEAYFGANGLILRNPTLINFLDGCAISLPCHRAGEAPV 426 Query: 424 GLSLAAAGGEDKRLLAIALAVEGLLAA 450 GLSLA GG+D+RLL++ALAVE LLA+ Sbjct: 427 GLSLAGLGGQDRRLLSVALAVEQLLAS 453 >ref|YP_004490413.1| Amidase [Delftia sp. Cs1-4] gb|AEF92058.1| Amidase [Delftia sp. Cs1-4] Length = 456 Score = 520 bits (1338), Expect = e-145, Method: Composition-based stats. Identities = 299/447 (66%), Positives = 347/447 (77%), Gaps = 2/447 (0%) Query: 4 TLSQLAADLDAGRITSLQLTEQALARIADPAGEGARVFTSVYADQARAAARASDTLRAAK 63 +L + L G TS+QLTEQALAR +GEG +VFT V+ A A ARASD LRAA Sbjct: 9 SLELASQRLHGGETTSVQLTEQALARTM--SGEGPKVFTRVFQGAALAEARASDVLRAAG 66 Query: 64 LCRSPLEGVPVSVKDLFDVAGETTLAGSVVLRGKPAATAHAAVVQNLMKAGAIVIGKTNM 123 L RSP+EG+PVSVKDLFD+AG TL GS +L P A A VVQ L +AGA+++G TNM Sbjct: 67 LARSPIEGLPVSVKDLFDIAGFPTLGGSRLLADAPPAQRTAEVVQRLRQAGAVIVGTTNM 126 Query: 124 TEFAYSGLGINPHYGTPQNTWERNVEGGRIPGGSSSGAAISVTDGMAFAAVGSDTGGSVR 183 TEFAYSGLGINPHYGTP+N W+R+ +GGRIPGGSSSGAAISVTDGMA AA+GSDTGGSVR Sbjct: 127 TEFAYSGLGINPHYGTPRNPWQRDEDGGRIPGGSSSGAAISVTDGMAMAAIGSDTGGSVR 186 Query: 184 IPSALNGLTGFKPTASRVSMQGVLPLSAYLDSIGPLAVSVECCAIMDAVLAGEAYVSVVA 243 IPSAL GLTGFKPTA RVSM+GVLPLSA LDSIGPLA SV CCA +DA+L+GE + A Sbjct: 187 IPSALCGLTGFKPTARRVSMEGVLPLSANLDSIGPLAPSVRCCATLDAILSGEPLGELHA 246 Query: 244 HPLRGLRLLAPTNVVLDAMDETVGKAYRAALDKLRAAGAVIVEAEVPAFNALAAINAKGG 303 PL+GLRLLAPTNVVLD MD TV A+ L L AGA I A V F LA IN+KGG Sbjct: 247 APLQGLRLLAPTNVVLDGMDATVAAAWERTLTLLSQAGAHITHAVVAPFGELADINSKGG 306 Query: 304 FTAAEAYAWHRALIAENAAGYDQRVVSRILRGKDMSAADLLDVLHARPRWIAEVQAQLAG 363 FTAAEA+AWHR IA + YD RV +RILRGKD+SAAD +D+L R +WIA V AQ+A Sbjct: 307 FTAAEAWAWHRHHIATRLSEYDPRVGTRILRGKDISAADYIDLLARRRQWIAAVSAQMAD 366 Query: 364 YDALVMPTVPIVAPRIAELQASDDAYYAANGKILRNPTLINFLDGCAVSLPCHAPGTAPV 423 +D +VMPTVP+VAP+IA+L SD+AY+ ANG ILRNPTLINFLDGCA+SLPC G APV Sbjct: 367 HDLIVMPTVPVVAPKIADLTQSDEAYFGANGLILRNPTLINFLDGCAISLPCQRAGEAPV 426 Query: 424 GLSLAAAGGEDKRLLAIALAVEGLLAA 450 GLSLA GG+D+RLL++ALAVE LLA+ Sbjct: 427 GLSLAGLGGQDRRLLSVALAVEQLLAS 453