BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.2134.p (512 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003775171.1| N-acyl-D-glutamate deacylase [Herbaspirillum... 881 0.0 ref|YP_004751930.1| N-acyl-D-amino-acid deacylase [Collimonas fu... 739 0.0 ref|YP_298220.1| N-acyl-D-amino-acid deacylase [Ralstonia eutrop... 706 0.0 ref|YP_586903.1| D-aminoacylase [Cupriavidus metallidurans CH34]... 698 0.0 ref|YP_004681376.1| D-aminoacylase Dan [Cupriavidus necator N-1]... 695 0.0 >ref|YP_003775171.1| N-acyl-D-glutamate deacylase [Herbaspirillum seropedicae SmR1] gb|ADJ63263.1| N-acyl-D-glutamate deacylase protein [Herbaspirillum seropedicae SmR1] Length = 496 Score = 881 bits (2276), Expect = 0.0, Method: Composition-based stats. Identities = 437/496 (88%), Positives = 464/496 (93%) Query: 17 MTSTLQSSGSPRHCDILIRHALVIDGSGAEPVQADVAVRNGRIVAVGQLAGWQAEQEIDG 76 M ST ++ R CDILIRHALVIDGSGAEPV ADVAV GR+ A+G+L WQA QEID Sbjct: 1 MLSTTHAADGERRCDILIRHALVIDGSGAEPVAADVAVHKGRVAAIGKLDDWQAAQEIDA 60 Query: 77 TARVLSPGFIDAHTHDDTNVIRTPEMFPKLSQGVTTVVVGNCGISAAPVTLKGDPPDPMN 136 + RVLSPGFID HTHDDTNVIRTPEMFPKLSQGVTTVVVGNCGISAAPVTLKG+PPDPMN Sbjct: 61 SGRVLSPGFIDVHTHDDTNVIRTPEMFPKLSQGVTTVVVGNCGISAAPVTLKGEPPDPMN 120 Query: 137 LLGQSTAFSYPTFASYVAAIGAARPSVNVAALVGHTALRSNHMDRLDRAASDAEIVGMRE 196 LLG+ AF YP+FASYV A+ AARP++NVAALVGHTALR+NHMDRLDRAA+++EI GMR Sbjct: 121 LLGKREAFHYPSFASYVEAVNAARPAINVAALVGHTALRNNHMDRLDRAATESEIAGMRA 180 Query: 197 QLTEALEHGALGLSTGLAYGNAINAPTEEVLALAEPLAEFGGLYATHLRSEFADILNAMD 256 QL EALEHGALGLSTGLAYGNAINAPTEEVLALAEPLAEFGGLYATHLRSEFADIL+AM+ Sbjct: 181 QLREALEHGALGLSTGLAYGNAINAPTEEVLALAEPLAEFGGLYATHLRSEFADILDAME 240 Query: 257 EAFRIGKHARVPVVISHLKCAGVENWGRSSEVLRALEAASRHQHVGCDCYPYTASSSTLD 316 E+FRIGKHARVPVVISHLKCAGVENWGRSSEVL ALE A+RHQHVGCDCYPYTASSSTLD Sbjct: 241 ESFRIGKHARVPVVISHLKCAGVENWGRSSEVLHALERAARHQHVGCDCYPYTASSSTLD 300 Query: 317 LKQVTADYDIQVTWSEPHPEQGGKMLKQIAADWGVDLHAAAARLMPAGAVYHCMSDDDVN 376 LKQVT DYDIQVTWSEPHPEQGGKMLKQIAA+W VDLHAAAARLMPAGAVYHCMSDDDVN Sbjct: 301 LKQVTGDYDIQVTWSEPHPEQGGKMLKQIAAEWDVDLHAAAARLMPAGAVYHCMSDDDVN 360 Query: 377 RILSHPATVVGSDGLPNDPLPHPRLWGAFPRVLGYYSREQKLFSLATAVHKMTGLSAQRF 436 RILSHPATVVGSDGLPNDPLPHPRLWGAFPRVLGYYSREQKLFSLATAVHKMTGLSAQRF Sbjct: 361 RILSHPATVVGSDGLPNDPLPHPRLWGAFPRVLGYYSREQKLFSLATAVHKMTGLSAQRF 420 Query: 437 GLEQRGFVREGYHADLVLFDPETIRDAASFTDPMQPAHGIEAVWVNGQLAYRGQDKQSTS 496 GL+ RGFVR GYHADLVLFD ET+RDAASFTDPMQPAHGIEAVWVNG+LAYRGQDK++T+ Sbjct: 421 GLDLRGFVRVGYHADLVLFDAETVRDAASFTDPMQPAHGIEAVWVNGELAYRGQDKKATA 480 Query: 497 ARAGRFLARTVQKTSA 512 ARAGRFLARTVQKTSA Sbjct: 481 ARAGRFLARTVQKTSA 496 >ref|YP_004751930.1| N-acyl-D-amino-acid deacylase [Collimonas fungivorans Ter331] gb|AEK61107.1| N-acyl-D-amino-acid deacylase [Collimonas fungivorans Ter331] Length = 511 Score = 739 bits (1908), Expect = 0.0, Method: Composition-based stats. Identities = 378/497 (76%), Positives = 415/497 (83%), Gaps = 9/497 (1%) Query: 18 TSTLQSSGSPRHCDILIRHALVIDGSGAEPVQADVAVRNGRIVAV--------GQLAGWQ 69 ++ L + G CD LIR+ VIDGSG+ ADVA+ GRI+ + G L+GWQ Sbjct: 5 SNALPTPGCTPSCDTLIRNVSVIDGSGSAAYVADVALAGGRILHIEPHGNASAGALSGWQ 64 Query: 70 AEQEIDGTARVLSPGFIDAHTHDDTNVIRTPEMFPKLSQGVTTVVVGNCGISAAPVTLKG 129 A+Q +DG+ +VLSPGFID HTHDDTNVIRTP M PKLSQGVTTVVVGNCGISAAPV+LK Sbjct: 65 AQQVVDGSGKVLSPGFIDVHTHDDTNVIRTPNMLPKLSQGVTTVVVGNCGISAAPVSLKA 124 Query: 130 DPPDPMNLLGQSTAFSYPTFASYVAAIGAARPSVNVAALVGHTALRSNHMDRLDRAASDA 189 +PPDPMNLLG+++AFSYPTFASYV A+ A+P++NVAAL+GHTALRSN MDRLDRAA+ Sbjct: 125 EPPDPMNLLGEASAFSYPTFASYVDAVNQAQPAINVAALIGHTALRSNQMDRLDRAATAT 184 Query: 190 EIVGMREQLTEALEHGALGLSTGLAYGNAINAPTEEVLALAEPLAEFGGLYATHLRSEFA 249 EI M EQL EALEHGALGLSTGLAYGNAINAPT EVLALAEPLAE G +YATHLRSEFA Sbjct: 185 EIAAMCEQLREALEHGALGLSTGLAYGNAINAPTAEVLALAEPLAEAGAIYATHLRSEFA 244 Query: 250 DILNAMDEAFRIGKHARVPVVISHLKCAGVENWGRSSEVLRALEAASRHQHVGCDCYPYT 309 DIL AMDEAFRIGKHARVPVVISHLKCAGVENWGRS EVL ALE A R+Q VGCDCYPYT Sbjct: 245 DILEAMDEAFRIGKHARVPVVISHLKCAGVENWGRSGEVLEALEQAQRYQPVGCDCYPYT 304 Query: 310 ASSSTLDLKQVTADYDIQVTWSEPHPEQGGKMLKQIAADWGVDLHAAAARLMPAGAVYHC 369 ASSSTLDLKQVT DI VTWSEP P GGK+L IAA+W VDL AAA RL PAGAVYHC Sbjct: 305 ASSSTLDLKQVTDTIDIMVTWSEPEPAMGGKLLADIAAEWQVDLMAAARRLQPAGAVYHC 364 Query: 370 MSDDDVNRILSHPATVVGSDGLPNDPLPHPRLWGAFPRVLGYYSREQKLFSLATAVHKMT 429 M DDDVNRILSHPA+VVGSDGLPNDPLPHPRLWGAFPRVLGYYSREQKLF LA A+ KMT Sbjct: 365 MQDDDVNRILSHPASVVGSDGLPNDPLPHPRLWGAFPRVLGYYSREQKLFPLAVAIRKMT 424 Query: 430 GLSAQRFGLEQRGFVREGYHADLVLFDPETIRDAASFTDPMQPAHGIEAVWVNGQLAYR- 488 GLSA+RFGL +RG VREGY ADLVLFD ET+RDAA+F+DPMQPA GI+AVWVNG L+Y Sbjct: 425 GLSAERFGLAERGLVREGYWADLVLFDAETVRDAATFSDPMQPAEGIDAVWVNGALSYLG 484 Query: 489 GQDKQSTSARAGRFLAR 505 G K T RAGRFLAR Sbjct: 485 GAQKAPTELRAGRFLAR 501 >ref|YP_298220.1| N-acyl-D-amino-acid deacylase [Ralstonia eutropha JMP134] gb|AAZ63376.1| N-acyl-D-amino-acid deacylase [Ralstonia eutropha JMP134] Length = 487 Score = 706 bits (1823), Expect = 0.0, Method: Composition-based stats. Identities = 363/488 (74%), Positives = 406/488 (83%), Gaps = 5/488 (1%) Query: 27 PRHCDILIRHALVIDGSGAEPVQADVAVRNGRIVAV---GQLAGWQAEQEIDGTARVLSP 83 P D LIR ++IDGSGA ADVA+R+GRI ++ G + A ++G VLSP Sbjct: 2 PHLFDTLIRCVMLIDGSGAAARMADVALRDGRIASIDDAGAIDADSAAHVVEGHGLVLSP 61 Query: 84 GFIDAHTHDDTNVIRTPEMFPKLSQGVTTVVVGNCGISAAPVTLKGDPPDPMNLLGQSTA 143 GFID HTHDDTNV+R PEM PKLSQGVTTV+VGNCGISAAPV L GDPPDPMNLLG + A Sbjct: 62 GFIDVHTHDDTNVVRQPEMTPKLSQGVTTVIVGNCGISAAPVVLSGDPPDPMNLLGHADA 121 Query: 144 FSYPTFASYVAAIGAARPSVNVAALVGHTALRSNHMDRLDRAASDAEIVGMREQLTEALE 203 F YP F SYV A+ AA+PSVNVAALVGHTALRSNHMDR DRAA+ AEI MR QL EAL+ Sbjct: 122 FRYPDFRSYVQAVDAAQPSVNVAALVGHTALRSNHMDRFDRAATPAEIAAMRAQLEEALQ 181 Query: 204 HGALGLSTGLAYGNAINAPTEEVLALAEPLAEFGGLYATHLRSEFADILNAMDEAFRIGK 263 HGALGLSTGLAY NA +APTEEVLALAEPLA G LYATHLRSEFA+IL+AMDEAFRIG+ Sbjct: 182 HGALGLSTGLAYANAFSAPTEEVLALAEPLAHAGALYATHLRSEFAEILDAMDEAFRIGR 241 Query: 264 HARVPVVISHLKCAGVENWGRSSEVLRALEAASRHQHVGCDCYPYTASSSTLDLKQVTAD 323 HARVPVVISHLKCAGV NWGRS+EVL AL+AA R Q VGCDCYPYTASSSTLDLKQVT+D Sbjct: 242 HARVPVVISHLKCAGVPNWGRSTEVLDALDAAQRWQPVGCDCYPYTASSSTLDLKQVTSD 301 Query: 324 YDIQVTWSEPHPEQGGKMLKQIAADWGVDLHAAAARLMPAGAVYHCMSDDDVNRILSHPA 383 +DI VTWSE PE GG++L +IAA+W VDLH AA RLMPAGAVYHCM D DV+RILSHPA Sbjct: 302 FDIMVTWSEGAPEMGGRLLAEIAAEWQVDLHEAARRLMPAGAVYHCMEDADVDRILSHPA 361 Query: 384 TVVGSDGLPNDPLPHPRLWGAFPRVLGYYSREQKLFSLATAVHKMTGLSAQRFGLEQRGF 443 TVVGSDGLPNDPLPHPRLWGAFPRVLG+Y+R+++LF LA AV+KMTG+SA+RFGL +RGF Sbjct: 362 TVVGSDGLPNDPLPHPRLWGAFPRVLGHYARDRELFPLAVAVNKMTGMSAERFGLTERGF 421 Query: 444 VREGYHADLVLFDPETIRDAASFTDPMQPAHGIEAVWVNGQLAYRGQDKQSTSARAGRFL 503 VREGY ADLVLFD TIRDAASFTDPMQPA GI AVWVNG+L++ Q +Q T RAGRFL Sbjct: 422 VREGYWADLVLFDANTIRDAASFTDPMQPAEGIAAVWVNGELSW--QQRQPTGVRAGRFL 479 Query: 504 ARTVQKTS 511 R Q + Sbjct: 480 PRGAQPAA 487 >ref|YP_586903.1| D-aminoacylase [Cupriavidus metallidurans CH34] gb|ABF11634.1| D-aminoacylase (N-acyl-D-amino-acid deacylase) [Cupriavidus metallidurans CH34] Length = 487 Score = 698 bits (1801), Expect = 0.0, Method: Composition-based stats. Identities = 357/486 (73%), Positives = 401/486 (82%), Gaps = 5/486 (1%) Query: 27 PRHCDILIRHALVIDGSGAEPVQADVAVRNGRIVAV---GQLAGWQAEQEIDGTARVLSP 83 P D LIR L++DGSGA ADVA+R+GRI + G + A ++G VLSP Sbjct: 2 PHLFDTLIRCVLLLDGSGAAARLADVAIRDGRIARIDEPGAIDPDSASNVVEGHGLVLSP 61 Query: 84 GFIDAHTHDDTNVIRTPEMFPKLSQGVTTVVVGNCGISAAPVTLKGDPPDPMNLLGQSTA 143 GFID HTHDDTNV+R P M PK+SQGVTTVVVGNCGISAAPVTL GDPPDPMNLLG++ A Sbjct: 62 GFIDVHTHDDTNVVREPAMTPKISQGVTTVVVGNCGISAAPVTLAGDPPDPMNLLGRADA 121 Query: 144 FSYPTFASYVAAIGAARPSVNVAALVGHTALRSNHMDRLDRAASDAEIVGMREQLTEALE 203 F YP F +YVAA+ AA+P+VNVAALVGHTALRSNHMDRLDR A++AE MR QL EAL+ Sbjct: 122 FRYPDFKTYVAAVNAAKPAVNVAALVGHTALRSNHMDRLDRPATEAETAAMRGQLQEALD 181 Query: 204 HGALGLSTGLAYGNAINAPTEEVLALAEPLAEFGGLYATHLRSEFADILNAMDEAFRIGK 263 HGALGLSTGLAY NA +APT+EVLALAEPLA+ G +YATHLRSEFA+IL+AMDEAFRIG+ Sbjct: 182 HGALGLSTGLAYANAFHAPTDEVLALAEPLAKAGAIYATHLRSEFAEILDAMDEAFRIGR 241 Query: 264 HARVPVVISHLKCAGVENWGRSSEVLRALEAASRHQHVGCDCYPYTASSSTLDLKQVTAD 323 HARVPVVISHLKCAGV NWGRS EVL ALE A R Q VGCDCYPYTASSSTLDLKQVT D Sbjct: 242 HARVPVVISHLKCAGVSNWGRSGEVLAALEGAERWQPVGCDCYPYTASSSTLDLKQVTND 301 Query: 324 YDIQVTWSEPHPEQGGKMLKQIAADWGVDLHAAAARLMPAGAVYHCMSDDDVNRILSHPA 383 +DIQVTWSEPHP GG++L IAA+W VDL AA RL PAGAVYHCM D DV+RILSHPA Sbjct: 302 FDIQVTWSEPHPTMGGRLLADIAAEWNVDLLEAARRLQPAGAVYHCMEDADVDRILSHPA 361 Query: 384 TVVGSDGLPNDPLPHPRLWGAFPRVLGYYSREQKLFSLATAVHKMTGLSAQRFGLEQRGF 443 TVVGSDGLPNDPLPHPRLWGAFPRVLG+Y+R+++LF LA AV+KMTG+SA+RFGL RGF Sbjct: 362 TVVGSDGLPNDPLPHPRLWGAFPRVLGHYARDRELFPLAVAVNKMTGMSAERFGLTGRGF 421 Query: 444 VREGYHADLVLFDPETIRDAASFTDPMQPAHGIEAVWVNGQLAYRGQDKQSTSARAGRFL 503 VREGY ADLVLFD TIRDAASFTDP QPA GI AVWVNG+L +R + +T RAGRFL Sbjct: 422 VREGYWADLVLFDAATIRDAASFTDPQQPAEGIAAVWVNGELTWR--QRAATGVRAGRFL 479 Query: 504 ARTVQK 509 R Q+ Sbjct: 480 PRGTQQ 485 >ref|YP_004681376.1| D-aminoacylase Dan [Cupriavidus necator N-1] gb|AEI80144.1| D-aminoacylase Dan [Cupriavidus necator N-1] Length = 490 Score = 695 bits (1794), Expect = 0.0, Method: Composition-based stats. Identities = 356/482 (73%), Positives = 402/482 (83%), Gaps = 5/482 (1%) Query: 27 PRHCDILIRHALVIDGSGAEPVQADVAVRNGRIVAV---GQLAGWQAEQEIDGTARVLSP 83 P D LIR +IDGSGA ADVA+R+GRI + G + A ++G VL+P Sbjct: 2 PHLFDTLIRCVTLIDGSGAAARLADVALRDGRIARIDEAGAIDPDAASHVVEGDGLVLAP 61 Query: 84 GFIDAHTHDDTNVIRTPEMFPKLSQGVTTVVVGNCGISAAPVTLKGDPPDPMNLLGQSTA 143 GFID HTHDDTNV+R PEM PKLSQGVTTVVVGNCGISAAPVTL G+PPDPMNLLG + A Sbjct: 62 GFIDVHTHDDTNVVRQPEMTPKLSQGVTTVVVGNCGISAAPVTLAGEPPDPMNLLGHADA 121 Query: 144 FSYPTFASYVAAIGAARPSVNVAALVGHTALRSNHMDRLDRAASDAEIVGMREQLTEALE 203 F YP F SYV A+ AA+P+VNVAALVGHTALRSNHMDR DRAA+ EI MR QL EAL+ Sbjct: 122 FRYPDFKSYVDAVEAAQPAVNVAALVGHTALRSNHMDRFDRAATPEEIAAMRAQLEEALQ 181 Query: 204 HGALGLSTGLAYGNAINAPTEEVLALAEPLAEFGGLYATHLRSEFADILNAMDEAFRIGK 263 HGALGLSTGLAY NA +APT+EVLALAEPLA+ G LYATHLRSEFA+IL+AMDEAFRIG+ Sbjct: 182 HGALGLSTGLAYANAFSAPTDEVLALAEPLAKAGALYATHLRSEFAEILDAMDEAFRIGR 241 Query: 264 HARVPVVISHLKCAGVENWGRSSEVLRALEAASRHQHVGCDCYPYTASSSTLDLKQVTAD 323 HARVPVVISHLKCAGV NWGRS+EVL AL+ A R Q VGCDCYPYTASSSTLDLKQVT+D Sbjct: 242 HARVPVVISHLKCAGVANWGRSTEVLDALDGAQRWQPVGCDCYPYTASSSTLDLKQVTSD 301 Query: 324 YDIQVTWSEPHPEQGGKMLKQIAADWGVDLHAAAARLMPAGAVYHCMSDDDVNRILSHPA 383 +DI VTWSE PE G++L IAA+W VDLH AA RLMPAGAVYHCM D DV+RILSHPA Sbjct: 302 FDIMVTWSEGAPEMSGRLLADIAAEWQVDLHEAARRLMPAGAVYHCMEDADVDRILSHPA 361 Query: 384 TVVGSDGLPNDPLPHPRLWGAFPRVLGYYSREQKLFSLATAVHKMTGLSAQRFGLEQRGF 443 TVVGSDGLPNDPLPHPRLWGAFPRVLG+Y+R+++LF L+ AV+KMTG+SA+RFGL++RGF Sbjct: 362 TVVGSDGLPNDPLPHPRLWGAFPRVLGHYARDRELFPLSVAVNKMTGMSAERFGLQERGF 421 Query: 444 VREGYHADLVLFDPETIRDAASFTDPMQPAHGIEAVWVNGQLAYRGQDKQSTSARAGRFL 503 VREGY ADLVLFD TIRDAASFTDPMQPA GI AVWVNG+L++ Q +Q+T RAGRFL Sbjct: 422 VREGYWADLVLFDATTIRDAASFTDPMQPAEGIAAVWVNGELSW--QQRQTTGVRAGRFL 479 Query: 504 AR 505 R Sbjct: 480 PR 481