BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.2074.p (444 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003775132.1| major facilitator superfamily permease [Herb... 767 0.0 ref|YP_004751978.1| major facilitator superfamily (MFS) multidru... 550 e-154 ref|YP_001888047.1| major facilitator superfamily protein [Burkh... 540 e-151 ref|YP_003910291.1| major facilitator superfamily protein [Burkh... 538 e-151 ref|ZP_06839778.1| major facilitator superfamily MFS_1 [Burkhold... 537 e-150 >ref|YP_003775132.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] gb|ADJ63224.1| permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 441 Score = 767 bits (1980), Expect = 0.0, Method: Composition-based stats. Identities = 431/441 (97%), Positives = 437/441 (99%) Query: 4 MDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKLGARKVFSWAMI 63 MDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKLGARKVFS AMI Sbjct: 1 MDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKLGARKVFSAAMI 60 Query: 64 VFMTGSILCAVSGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSELVKAVSYLTLPA 123 VFMTGSILCAVSGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSELVKAVSYLTLPA Sbjct: 61 VFMTGSILCAVSGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSELVKAVSYLTLPA 120 Query: 124 LLGPVVGPLLGGFLTTYFHWRWVFLINIPFCFLGLYLAKRFIQNFKEDNPAPLDVKGFVL 183 LLGPVVGPLLGGF+TTYFHWRWVFLINIPFCFLGLYLA+RFIQNFKEDNPAPLDVKGFVL Sbjct: 121 LLGPVVGPLLGGFITTYFHWRWVFLINIPFCFLGLYLARRFIQNFKEDNPAPLDVKGFVL 180 Query: 184 TSVGAAMIMLGLSLFGSQLVDGATPVAMCSIGAIALVMYYRHSTQIPYPLLDFRLLRIPT 243 TSVGAAMIMLGLSLFGS LVDGATPVAMCSIGAIALVMYYRHSTQI +PLLDFRLLRIPT Sbjct: 181 TSVGAAMIMLGLSLFGSHLVDGATPVAMCSIGAIALVMYYRHSTQIEFPLLDFRLLRIPT 240 Query: 244 LHASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAFQAGSITCASAFGSLFIRLLASRVLR 303 LHASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAFQAGSITCASAFGSLFIRLLA RVLR Sbjct: 241 LHASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAFQAGSITCASAFGSLFIRLLAGRVLR 300 Query: 304 RYGFRTVLMVNAVFAGIALAAYGIFSMHTPYLLIVTIVLMGGVFPALQFTCLNSIAYADI 363 RYGFRTVLMVNAVFAGIALAAYGIFS+HTPYLLIV+IVLMGGVFPALQFTCLNSIAYADI Sbjct: 301 RYGFRTVLMVNAVFAGIALAAYGIFSIHTPYLLIVSIVLMGGVFPALQFTCLNSIAYADI 360 Query: 364 DSRDAGRVTSLASVIQQLSLGLGVTVAGIVLQLSNHLQGHASIVEADFWPAFLVIGLFSV 423 DSRDAGRVTSLASVIQQLSLGLGVTVAGIVLQLSNHLQGHA+IVEADFWPAFLVIGLFSV Sbjct: 361 DSRDAGRVTSLASVIQQLSLGLGVTVAGIVLQLSNHLQGHATIVEADFWPAFLVIGLFSV 420 Query: 424 ASVPISGKLPHNAGLELSRGK 444 ASVPISGKLPHNAGLELSRGK Sbjct: 421 ASVPISGKLPHNAGLELSRGK 441 >ref|YP_004751978.1| major facilitator superfamily (MFS) multidrug efflux pump [Collimonas fungivorans Ter331] gb|AEK61155.1| major facilitator superfamily (MFS) multidrug efflux pump [Collimonas fungivorans Ter331] Length = 470 Score = 550 bits (1417), Expect = e-154, Method: Composition-based stats. Identities = 288/444 (64%), Positives = 371/444 (83%) Query: 1 MENMDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKLGARKVFSW 60 MENMDATV+ATSLP LARDL QDP+TLKLA+T+YV LGVFIPISGW+AD+ GAR VF Sbjct: 25 MENMDATVIATSLPVLARDLGQDPLTLKLALTSYVVGLGVFIPISGWVADRFGARTVFRA 84 Query: 61 AMIVFMTGSILCAVSGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSELVKAVSYLT 120 A++VF++GS++CA S SL FVAARFLQGIGGAMMVPVGR++I RSV +++LVKA+SYLT Sbjct: 85 AILVFLSGSLMCAASSSLAAFVAARFLQGIGGAMMVPVGRIVIFRSVPRTDLVKAISYLT 144 Query: 121 LPALLGPVVGPLLGGFLTTYFHWRWVFLINIPFCFLGLYLAKRFIQNFKEDNPAPLDVKG 180 +P+ LGPV+GP LGGF+TTYFHWRW+FLIN+P LG+YLA R+I+N++ D+ PLD+KG Sbjct: 145 IPSQLGPVIGPPLGGFITTYFHWRWIFLINVPISILGMYLASRYIENYRSDDLQPLDMKG 204 Query: 181 FVLTSVGAAMIMLGLSLFGSQLVDGATPVAMCSIGAIALVMYYRHSTQIPYPLLDFRLLR 240 FVL+++G+ ++MLGLSL +L+ AMC +G + L +Y H+ + P+PLLD RLLR Sbjct: 205 FVLSAIGSTLLMLGLSLIDGELLAPEWAFAMCVLGGLTLYVYLLHARREPFPLLDLRLLR 264 Query: 241 IPTLHASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAFQAGSITCASAFGSLFIRLLASR 300 IPT ASV+GGSLFRIGLGAVPFLLPLALQ G GM AF AG+ITCASAFG++F++ + + Sbjct: 265 IPTFRASVIGGSLFRIGLGAVPFLLPLALQVGFGMNAFHAGTITCASAFGAIFMKAIGTS 324 Query: 301 VLRRYGFRTVLMVNAVFAGIALAAYGIFSMHTPYLLIVTIVLMGGVFPALQFTCLNSIAY 360 VLRRYGFR+VL+ NAV AG+ALA+YG+F+ TPYL+++ +VL+GG FP++QFTCLN++AY Sbjct: 325 VLRRYGFRSVLIWNAVLAGLALASYGLFTPTTPYLVMMAVVLLGGFFPSMQFTCLNTMAY 384 Query: 361 ADIDSRDAGRVTSLASVIQQLSLGLGVTVAGIVLQLSNHLQGHASIVEADFWPAFLVIGL 420 AD+DS D R TSLASV+QQ+SLGLGVT+ G+ + LS+ LQGH++IV ADFWPAF+VIGL Sbjct: 385 ADLDSADVSRATSLASVVQQISLGLGVTIGGLAVHLSSRLQGHSTIVAADFWPAFIVIGL 444 Query: 421 FSVASVPISGKLPHNAGLELSRGK 444 FS+AS+P++ LP NAG L+ K Sbjct: 445 FSLASIPVTRHLPLNAGSALTGHK 468 >ref|YP_001888047.1| major facilitator superfamily protein [Burkholderia phytofirmans PsJN] gb|ACD18677.1| major facilitator superfamily MFS_1 [Burkholderia phytofirmans PsJN] Length = 467 Score = 540 bits (1392), Expect = e-151, Method: Composition-based stats. Identities = 293/443 (66%), Positives = 361/443 (81%) Query: 1 MENMDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKLGARKVFSW 60 MEN+D TV+ TSLP LARDL QDPITLKLA+TAYV LGVFIPI GW+AD+ G+R VF Sbjct: 20 MENVDGTVIVTSLPVLARDLGQDPITLKLAVTAYVIGLGVFIPICGWVADRFGSRTVFRA 79 Query: 61 AMIVFMTGSILCAVSGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSELVKAVSYLT 120 A+ VFM GS++CA S SL TFV ARF+QGIGGAMMVPVGR+II RSV KS+ ++AV+YLT Sbjct: 80 AIGVFMAGSLMCAASTSLGTFVVARFVQGIGGAMMVPVGRIIIFRSVPKSDFIRAVNYLT 139 Query: 121 LPALLGPVVGPLLGGFLTTYFHWRWVFLINIPFCFLGLYLAKRFIQNFKEDNPAPLDVKG 180 +PALLGPVVGP LGGF+TTY HWR +F INIP LG++LA + I N +E +P LD G Sbjct: 140 VPALLGPVVGPPLGGFITTYLHWRLIFFINIPIGLLGIWLANKHIANVREAHPGRLDWTG 199 Query: 181 FVLTSVGAAMIMLGLSLFGSQLVDGATPVAMCSIGAIALVMYYRHSTQIPYPLLDFRLLR 240 F L++ GA++ MLGLSL G +LV V MC IG + L +Y +++++ P+LD RLLR Sbjct: 200 FFLSASGASLFMLGLSLVGGELVSNTVSVGMCVIGVVLLTLYALYASRVELPVLDLRLLR 259 Query: 241 IPTLHASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAFQAGSITCASAFGSLFIRLLASR 300 IP+ HASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAF++GSITCASAFGS+F++ ASR Sbjct: 260 IPSFHASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAFKSGSITCASAFGSIFMKAAASR 319 Query: 301 VLRRYGFRTVLMVNAVFAGIALAAYGIFSMHTPYLLIVTIVLMGGVFPALQFTCLNSIAY 360 +L R+GFRTVLM NA AG+A+A YG+F TP+ LI +VL GG FP+LQFT LN++AY Sbjct: 320 ILERFGFRTVLMFNAGCAGLAIAVYGLFFPGTPHWLIWCVVLFGGFFPSLQFTSLNTLAY 379 Query: 361 ADIDSRDAGRVTSLASVIQQLSLGLGVTVAGIVLQLSNHLQGHASIVEADFWPAFLVIGL 420 ADI SRD GR TS+ASVIQQ+SLGLGVT+AGIVLQ+S+++QGH++IV +DFWPAFLV+GL Sbjct: 380 ADIPSRDVGRATSVASVIQQISLGLGVTIAGIVLQISHNVQGHSAIVFSDFWPAFLVVGL 439 Query: 421 FSVASVPISGKLPHNAGLELSRG 443 FS S+PI+ +LPH AG E++RG Sbjct: 440 FSFMSIPITARLPHGAGDEIARG 462 >ref|YP_003910291.1| major facilitator superfamily protein [Burkholderia sp. CCGE1003] gb|ADN61000.1| major facilitator superfamily MFS_1 [Burkholderia sp. CCGE1003] Length = 467 Score = 538 bits (1387), Expect = e-151, Method: Composition-based stats. Identities = 289/444 (65%), Positives = 361/444 (81%) Query: 1 MENMDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKLGARKVFSW 60 MEN+D TV+ TSLP LARDL QDPITLKLA+TAYV LGVFIPI GW+AD+ G+R VF Sbjct: 20 MENVDGTVIVTSLPVLARDLGQDPITLKLAVTAYVIGLGVFIPICGWVADRFGSRTVFRT 79 Query: 61 AMIVFMTGSILCAVSGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSELVKAVSYLT 120 A+ +FM GS++CA S SL FV ARF+QGIGGAMMVPVGR+II RSV KS+ ++AV+YLT Sbjct: 80 AIGIFMAGSLMCAASTSLGMFVVARFVQGIGGAMMVPVGRIIIFRSVPKSDFIRAVNYLT 139 Query: 121 LPALLGPVVGPLLGGFLTTYFHWRWVFLINIPFCFLGLYLAKRFIQNFKEDNPAPLDVKG 180 +PALLGPVVGP LGGF+TTY HWR +F +NIP LG++LA + I N +E +P PLD G Sbjct: 140 VPALLGPVVGPPLGGFITTYLHWRLIFFVNIPIGLLGIWLANKHIANVREAHPGPLDWVG 199 Query: 181 FVLTSVGAAMIMLGLSLFGSQLVDGATPVAMCSIGAIALVMYYRHSTQIPYPLLDFRLLR 240 F+L++ GA++ MLGLSL G +LV A MC++G + LV+Y ++ ++ P+LD RLLR Sbjct: 200 FLLSASGASLFMLGLSLVGGELVPNAVSFGMCAVGVVLLVIYVLYANRVDMPVLDLRLLR 259 Query: 241 IPTLHASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAFQAGSITCASAFGSLFIRLLASR 300 IP+ HASV GGSLFRIGLGAVPFLLPLALQEGLGMTAF +GSITCASAFGS+F++ ASR Sbjct: 260 IPSFHASVAGGSLFRIGLGAVPFLLPLALQEGLGMTAFVSGSITCASAFGSIFMKAAASR 319 Query: 301 VLRRYGFRTVLMVNAVFAGIALAAYGIFSMHTPYLLIVTIVLMGGVFPALQFTCLNSIAY 360 +L R+GFRTVLM NA AG+A+A YG+F TP+ LI +VL GG FP+LQFT LN++AY Sbjct: 320 ILERFGFRTVLMFNAGCAGVAIAIYGLFFPGTPHWLIWCVVLFGGFFPSLQFTSLNTLAY 379 Query: 361 ADIDSRDAGRVTSLASVIQQLSLGLGVTVAGIVLQLSNHLQGHASIVEADFWPAFLVIGL 420 ADI SRD GR TS+ASVIQQ+SLGLGVT+AGIVLQ+S++LQGH IV +DFWPAF+V+GL Sbjct: 380 ADIPSRDVGRATSVASVIQQISLGLGVTIAGIVLQISHNLQGHTRIVFSDFWPAFVVVGL 439 Query: 421 FSVASVPISGKLPHNAGLELSRGK 444 FS S+P++ +LPH+AG E++RG+ Sbjct: 440 FSFLSIPVTARLPHDAGDEIARGR 463 >ref|ZP_06839778.1| major facilitator superfamily MFS_1 [Burkholderia sp. Ch1-1] gb|EFG72608.1| major facilitator superfamily MFS_1 [Burkholderia sp. Ch1-1] Length = 467 Score = 537 bits (1384), Expect = e-150, Method: Composition-based stats. Identities = 289/443 (65%), Positives = 359/443 (81%) Query: 1 MENMDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKLGARKVFSW 60 MEN+D TV+ TSLP LARDL QDPIT KLA+TAYV LGVFIPI GW+AD+ G+R VF Sbjct: 20 MENVDGTVIVTSLPVLARDLGQDPITFKLAVTAYVIGLGVFIPICGWVADRFGSRTVFRT 79 Query: 61 AMIVFMTGSILCAVSGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSELVKAVSYLT 120 A+ VFM GS++CA S SL TFV ARF+QGIGGAMMVPVGR+II RSV KS+ ++AV+YLT Sbjct: 80 AIGVFMAGSLMCAASTSLGTFVVARFVQGIGGAMMVPVGRIIIFRSVPKSDFIRAVNYLT 139 Query: 121 LPALLGPVVGPLLGGFLTTYFHWRWVFLINIPFCFLGLYLAKRFIQNFKEDNPAPLDVKG 180 +PALLGPVVGP LGGF+TTY HWR +F +NIP LG++LA + I N +E +P LD G Sbjct: 140 VPALLGPVVGPPLGGFITTYLHWRLIFFVNIPIGLLGIWLANKHIANVREAHPGRLDWTG 199 Query: 181 FVLTSVGAAMIMLGLSLFGSQLVDGATPVAMCSIGAIALVMYYRHSTQIPYPLLDFRLLR 240 F L++ GA++ MLGLSL G +LV V MC G + L +Y ++ ++ P+LD RLLR Sbjct: 200 FALSASGASLFMLGLSLVGGELVSNTVSVGMCVAGVVLLTLYVLYANRVELPVLDLRLLR 259 Query: 241 IPTLHASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAFQAGSITCASAFGSLFIRLLASR 300 IP+ HASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAF++G+ITCASAFGS+F++ ASR Sbjct: 260 IPSFHASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAFKSGAITCASAFGSIFMKAAASR 319 Query: 301 VLRRYGFRTVLMVNAVFAGIALAAYGIFSMHTPYLLIVTIVLMGGVFPALQFTCLNSIAY 360 +L R+GFRTVLM NA AG+A+A YG+F TP+ LI +VL GGVFP+LQFT LN++AY Sbjct: 320 ILERFGFRTVLMFNAGCAGLAIAVYGLFFPGTPHWLIWCVVLFGGVFPSLQFTSLNTLAY 379 Query: 361 ADIDSRDAGRVTSLASVIQQLSLGLGVTVAGIVLQLSNHLQGHASIVEADFWPAFLVIGL 420 ADI SRD GR TS+ASVIQQ+SLGLGVT+AGIVLQ+S+++QGH++IV +DFWPAFLV+GL Sbjct: 380 ADIPSRDVGRATSVASVIQQISLGLGVTIAGIVLQISHNVQGHSAIVFSDFWPAFLVVGL 439 Query: 421 FSVASVPISGKLPHNAGLELSRG 443 FS S+P++ +LPH AG E++RG Sbjct: 440 FSFMSIPVTARLPHGAGDEIARG 462