BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.2016.p (292 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_581804.1| 5-carboxymethyl-2-hydroxymuconate delta-isomera... 448 e-124 ref|NP_890666.1| enzyme [Bordetella bronchiseptica RB50] >gi|335... 422 e-116 ref|YP_621111.1| 5-carboxymethyl-2-hydroxymuconate delta-isomera... 421 e-116 ref|NP_885853.1| putative enzyme [Bordetella parapertussis 12822... 421 e-116 ref|ZP_02885011.1| 5-carboxymethyl-2-hydroxymuconate Delta-isome... 408 e-112 >ref|YP_581804.1| 5-carboxymethyl-2-hydroxymuconate delta-isomerase [Cupriavidus metallidurans CH34] gb|ABF12854.1| 5-carboxymethyl-2-hydroxymuconate delta-isomerase [Cupriavidus metallidurans CH34] Length = 292 Score = 448 bits (1153), Expect = e-124, Method: Composition-based stats. Identities = 214/291 (73%), Positives = 247/291 (84%) Query: 1 MKFATVQYAGKEQVALVDGNDGVFWPLSSLDVDFSGNMIQLVQKFADLRGRLSPKGKGVP 60 MKFAT+Q A + V+LV+ +WPL L F+G+M+QLV F +++GRL+P G+G P Sbjct: 1 MKFATLQTAQGKLVSLVNTESQQYWPLQDLVSGFTGDMVQLVHVFPEIKGRLTPTGEGKP 60 Query: 61 LSEAKVLAPIPVPARNIFCVGKNYREHAAEFSKSGFDSSAKEGEHVPEAPVIFTKPASTV 120 LS+A VLAPI P RNIFCVGKNY EHA EFSKSGFDSSAKEGEH PEAPV+FTKPASTV Sbjct: 61 LSQATVLAPIDQPRRNIFCVGKNYHEHAKEFSKSGFDSSAKEGEHAPEAPVVFTKPASTV 120 Query: 121 IATGEKVPSHPKVTQQLDYEAELAVIIGVGGSNISKEDAYRHVWGYTIVNDFTARDLQKL 180 I G+KVPSHP VT+QLDYEAELAV+IG G I KE+AY+HV GYTIVNDFTARDLQK Sbjct: 121 IGPGKKVPSHPNVTKQLDYEAELAVVIGKAGRAIRKEEAYKHVIGYTIVNDFTARDLQKT 180 Query: 181 HRQWFLGKSLDGFCPMGPYAVTADEVNPENLNVRCWINGELRQDASTADLIFDIPSLIET 240 HRQWFLGKSLDGFCPMGPY VTADE++ ENL V+CWIN ELRQD++T+ LIFDIP+LIET Sbjct: 181 HRQWFLGKSLDGFCPMGPYLVTADELDVENLEVKCWINDELRQDSNTSQLIFDIPTLIET 240 Query: 241 ISAGIELRPGDIIATGTPAGVGIGFNPPKFLKSGDKMRIEISGLGVLENEV 291 +SAGIEL+PGD+IATGTPAGVGIGFNPPKF+K+GD MRIEI+GLG L NEV Sbjct: 241 LSAGIELQPGDVIATGTPAGVGIGFNPPKFVKAGDVMRIEITGLGTLVNEV 291 >ref|NP_890666.1| enzyme [Bordetella bronchiseptica RB50] emb|CAE34495.1| putative enzyme [Bordetella bronchiseptica RB50] Length = 293 Score = 422 bits (1085), Expect = e-116, Method: Composition-based stats. Identities = 198/291 (68%), Positives = 238/291 (81%), Gaps = 1/291 (0%) Query: 1 MKFATVQYAGKEQVALVDGNDGVFWPLSSLDVDFSGNMIQLVQKFADLRGRLSPKGKGVP 60 MKFA++ Y GK QVA++D GVFWPLS L D G++++L+ + + R++P G G+ Sbjct: 1 MKFASLDYQGKAQVAILDTEAGVFWPLSGLLRD-EGDLVELISAWDSVHQRIAPSGAGIA 59 Query: 61 LSEAKVLAPIPVPARNIFCVGKNYREHAAEFSKSGFDSSAKEGEHVPEAPVIFTKPASTV 120 L A +LAPI P RNIFCVGKNY EHAAEF +SGFDSSAK+GEH PEAPV+FTKPAS + Sbjct: 60 LDRATILAPISSPRRNIFCVGKNYHEHAAEFQRSGFDSSAKDGEHAPEAPVVFTKPASAI 119 Query: 121 IATGEKVPSHPKVTQQLDYEAELAVIIGVGGSNISKEDAYRHVWGYTIVNDFTARDLQKL 180 +A G +P H +VTQQLDYEAELAV+IG G I K DA HV+GYTI+ND TARDLQKL Sbjct: 120 VAPGAVIPRHAQVTQQLDYEAELAVVIGKAGQGIKKADAMSHVFGYTIINDVTARDLQKL 179 Query: 181 HRQWFLGKSLDGFCPMGPYAVTADEVNPENLNVRCWINGELRQDASTADLIFDIPSLIET 240 HRQWF+GKSLDGFCPMGPY VTADEV+ + L+VRCWIN ELRQ+A+T LIFDIP+LIET Sbjct: 180 HRQWFIGKSLDGFCPMGPYIVTADEVDGQALDVRCWINDELRQNANTRQLIFDIPTLIET 239 Query: 241 ISAGIELRPGDIIATGTPAGVGIGFNPPKFLKSGDKMRIEISGLGVLENEV 291 +SAG+ L+PGDIIATGTPAGVGIGF PP+FL+SGD++RIEI+GLGVLEN V Sbjct: 240 LSAGMTLQPGDIIATGTPAGVGIGFTPPRFLQSGDRVRIEIAGLGVLENTV 290 >ref|YP_621111.1| 5-carboxymethyl-2-hydroxymuconate delta-isomerase [Burkholderia cenocepacia AU 1054] ref|YP_840224.1| 5-carboxymethyl-2-hydroxymuconate delta-isomerase [Burkholderia cenocepacia HI2424] gb|ABF76138.1| 5-carboxymethyl-2-hydroxymuconate delta-isomerase [Burkholderia cenocepacia AU 1054] gb|ABK13331.1| 5-carboxymethyl-2-hydroxymuconate delta-isomerase [Burkholderia cenocepacia HI2424] Length = 293 Score = 421 bits (1083), Expect = e-116, Method: Composition-based stats. Identities = 196/291 (67%), Positives = 237/291 (81%) Query: 1 MKFATVQYAGKEQVALVDGNDGVFWPLSSLDVDFSGNMIQLVQKFADLRGRLSPKGKGVP 60 MK A + AGK+ +++VD FWP+ SL F G+M+QLVQ+F +++ L P G G+ Sbjct: 1 MKLANLNVAGKKTISIVDIVHSRFWPVQSLVPQFDGDMVQLVQQFEEVKPHLKPTGNGLS 60 Query: 61 LSEAKVLAPIPVPARNIFCVGKNYREHAAEFSKSGFDSSAKEGEHVPEAPVIFTKPASTV 120 L+EA +LAPI P RNIFCVGKNY EHAAEF SGFDSSAK GEH PEAPVIFTKPASTV Sbjct: 61 LAEATILAPIDQPRRNIFCVGKNYHEHAAEFQNSGFDSSAKNGEHAPEAPVIFTKPASTV 120 Query: 121 IATGEKVPSHPKVTQQLDYEAELAVIIGVGGSNISKEDAYRHVWGYTIVNDFTARDLQKL 180 I K+P H VT QLDYEAEL V+IG GG I K DA +V+GYT++NDFTARDLQKL Sbjct: 121 IGPSAKIPRHVGVTAQLDYEAELGVVIGKGGRGIKKADAMNYVFGYTVINDFTARDLQKL 180 Query: 181 HRQWFLGKSLDGFCPMGPYAVTADEVNPENLNVRCWINGELRQDASTADLIFDIPSLIET 240 HRQWFLGKSLDGFCPMGP+ +T+DEVN +N++++CW+NGELRQ+++T+ LIFDIP+LIET Sbjct: 181 HRQWFLGKSLDGFCPMGPFVITSDEVNGQNIDIKCWVNGELRQNSNTSKLIFDIPTLIET 240 Query: 241 ISAGIELRPGDIIATGTPAGVGIGFNPPKFLKSGDKMRIEISGLGVLENEV 291 +SAGIEL+PGD+IATGTPAGVGIGFNPPKFL+ GD +RIEI +GVLENEV Sbjct: 241 LSAGIELQPGDVIATGTPAGVGIGFNPPKFLQKGDVIRIEIQNVGVLENEV 291 >ref|NP_885853.1| putative enzyme [Bordetella parapertussis 12822] emb|CAE38981.1| putative enzyme [Bordetella parapertussis] Length = 293 Score = 421 bits (1081), Expect = e-116, Method: Composition-based stats. Identities = 197/291 (67%), Positives = 238/291 (81%), Gaps = 1/291 (0%) Query: 1 MKFATVQYAGKEQVALVDGNDGVFWPLSSLDVDFSGNMIQLVQKFADLRGRLSPKGKGVP 60 MKFA++ Y GK QVA++D GVFWPLS L D G++++L+ + + R++P G G+ Sbjct: 1 MKFASLDYQGKAQVAILDTEAGVFWPLSGLLRD-EGDLVELISAWDSVHQRIAPSGAGIA 59 Query: 61 LSEAKVLAPIPVPARNIFCVGKNYREHAAEFSKSGFDSSAKEGEHVPEAPVIFTKPASTV 120 L A +LAPI P RNIFCVGKNY EHAAEF +SGFDSSAK+GEH PEAPV+FTKPAS + Sbjct: 60 LDRATILAPISSPRRNIFCVGKNYHEHAAEFQRSGFDSSAKDGEHAPEAPVVFTKPASAI 119 Query: 121 IATGEKVPSHPKVTQQLDYEAELAVIIGVGGSNISKEDAYRHVWGYTIVNDFTARDLQKL 180 +A G +P H +VTQQLDYEA+LAV+IG G I K DA HV+GYTI+ND TARDLQKL Sbjct: 120 VAPGAVIPRHAQVTQQLDYEADLAVVIGKAGQGIKKADAMSHVFGYTIINDVTARDLQKL 179 Query: 181 HRQWFLGKSLDGFCPMGPYAVTADEVNPENLNVRCWINGELRQDASTADLIFDIPSLIET 240 HRQWF+GKSLDGFCPMGPY VTADEV+ + L+VRCWIN ELRQ+A+T LIFDIP+LIET Sbjct: 180 HRQWFIGKSLDGFCPMGPYIVTADEVDGQALDVRCWINDELRQNANTRQLIFDIPTLIET 239 Query: 241 ISAGIELRPGDIIATGTPAGVGIGFNPPKFLKSGDKMRIEISGLGVLENEV 291 +SAG+ L+PGDIIATGTPAGVGIGF PP+FL+SGD++RIEI+GLGVLEN V Sbjct: 240 LSAGMTLQPGDIIATGTPAGVGIGFTPPRFLQSGDRVRIEIAGLGVLENTV 290 >ref|ZP_02885011.1| 5-carboxymethyl-2-hydroxymuconate Delta-isomerase [Burkholderia graminis C4D1M] gb|EDT09443.1| 5-carboxymethyl-2-hydroxymuconate Delta-isomerase [Burkholderia graminis C4D1M] Length = 292 Score = 408 bits (1049), Expect = e-112, Method: Composition-based stats. Identities = 191/291 (65%), Positives = 231/291 (79%) Query: 1 MKFATVQYAGKEQVALVDGNDGVFWPLSSLDVDFSGNMIQLVQKFADLRGRLSPKGKGVP 60 MK A + G + VA+VD +WP++ L F G+MIQLV ++ +R L PK G P Sbjct: 1 MKLANLLVNGAKTVAVVDPERQQYWPVAELSDGFRGDMIQLVHDYSSIRSALVPKHDGRP 60 Query: 61 LSEAKVLAPIPVPARNIFCVGKNYREHAAEFSKSGFDSSAKEGEHVPEAPVIFTKPASTV 120 LS VLAPI P RNIFCVGKNY +HA EFSKSGFD SA+ GEH+PEAPV+FTKPA+TV Sbjct: 61 LSGVTVLAPIDQPRRNIFCVGKNYHDHAEEFSKSGFDKSAQAGEHIPEAPVVFTKPATTV 120 Query: 121 IATGEKVPSHPKVTQQLDYEAELAVIIGVGGSNISKEDAYRHVWGYTIVNDFTARDLQKL 180 I G+K+P H VTQQLDYE E+ V+IG G I K+DA HV+GYT++ND TARDLQKL Sbjct: 121 IGPGDKIPRHAAVTQQLDYEVEIGVVIGKAGRGIRKQDALAHVFGYTLINDMTARDLQKL 180 Query: 181 HRQWFLGKSLDGFCPMGPYAVTADEVNPENLNVRCWINGELRQDASTADLIFDIPSLIET 240 HRQWFLGKSLDGFCPMGPY VTADE++ +++VRCW+NGELRQ +++ LIFDIP+LIET Sbjct: 181 HRQWFLGKSLDGFCPMGPYLVTADELDANDIDVRCWVNGELRQSSNSRHLIFDIPTLIET 240 Query: 241 ISAGIELRPGDIIATGTPAGVGIGFNPPKFLKSGDKMRIEISGLGVLENEV 291 ISAGIEL+PGD+IATGTPAGVGIGFNPP+FL+SGD +R+EI +GVLENEV Sbjct: 241 ISAGIELQPGDVIATGTPAGVGIGFNPPRFLQSGDVVRMEIPRVGVLENEV 291