BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.2011.p (446 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_770945.1| major facilitator transporter [Rhizobium legumi... 283 6e-74 ref|YP_004215865.1| major facilitator superfamily MFS_1 [Rahnell... 279 7e-73 ref|ZP_06975051.1| major facilitator superfamily MFS_1 [Ktedonob... 263 5e-68 ref|YP_430703.1| major facilitator transporter [Moorella thermoa... 254 2e-65 ref|YP_002471090.1| hypothetical protein CKR_0625 [Clostridium k... 249 8e-64 >ref|YP_770945.1| major facilitator transporter [Rhizobium leguminosarum bv. viciae 3841] emb|CAK02847.1| putative major facilitator superfamily (MFS) transporter transmembrane component [Rhizobium leguminosarum bv. viciae 3841] Length = 404 Score = 283 bits (723), Expect = 6e-74, Method: Composition-based stats. Identities = 187/369 (50%), Positives = 252/369 (68%), Gaps = 1/369 (0%) Query: 49 KTSSYANQRTGNISMMTAACGIAVANVYLCQPLLPAISASFSITSEQAGRVATAAQIGYA 108 +++ ++ G + M+ AACGI V NVYLCQPLL ++ S + + AG VA AQ+GYA Sbjct: 4 ESTPHSQLTRGTLPMLAAACGITVGNVYLCQPLLDQMAVSLRVPEQTAGLVAVGAQVGYA 63 Query: 109 LGILLVVPLADRVSVTKLIRVLLALSAFFLLSAAAAPNIEILTLASLAITVATVIPQILI 168 LGIL V+PLAD ++ +L+R LL L++ FLL+AA + +L AS+A+T +TV+PQILI Sbjct: 64 LGILFVLPLADVIASRRLVRTLLVLTSLFLLAAAFSSRTSLLAAASVALTASTVVPQILI 123 Query: 169 PIAVSTSSPEKAGSVVGTLQSGLIAGILLSRTFSGLIAQYSGSWRFSYVAAAACTVVLLL 228 PI ++PE G +G LQ+GLI GILLSRT SG +AQ +G+WR Y+ AA T +L+L Sbjct: 124 PIVSGMTAPEHRGRTIGALQTGLILGILLSRTASGSLAQVTGTWRSPYLLAAVLTGLLVL 183 Query: 229 TLPRALPKRELPPVNISYFALIGSLPGLVSKWTHLRLSALLGALVFGAFSAFWATLAFHL 288 +PR +P+RE P + Y +L+ SL + + LRLS LG LVFGAFSA WATLAF+L Sbjct: 184 IVPRLIPERETKPRHTGYLSLLRSL-PPLLQHRPLRLSMTLGFLVFGAFSALWATLAFYL 242 Query: 289 AEQPFGYGSAEAGLFGLWGAAGALIAPLFGKIADRHGPSILNVISITAAALAFACFAGYG 348 A FG+G A AGLFGL+GA GA++AP+ G+++DR G S +N++S+ A+ +A A G Sbjct: 243 AGPDFGFGPATAGLFGLYGAPGAILAPMAGRLSDRVGSSKINLVSLAASGIALALAGWLG 302 Query: 349 NIYVAAIVIGVNFLDFANQAGQIANQTRIFKLDPTARARLNTVYMVATFGGGAVGSTLGS 408 + +V+ VN LDF Q+GQIANQTRI L RARLNT+YM ATFGGGA GS G Sbjct: 303 GGSLLILVVAVNLLDFGLQSGQIANQTRILGLGDDIRARLNTLYMAATFGGGAAGSFAGM 362 Query: 409 YAWSKHQWS 417 AWS W+ Sbjct: 363 LAWSFGGWT 371 >ref|YP_004215865.1| major facilitator superfamily MFS_1 [Rahnella sp. Y9602] gb|ADW76738.1| major facilitator superfamily MFS_1 [Rahnella sp. Y9602] Length = 406 Score = 279 bits (713), Expect = 7e-73, Method: Composition-based stats. Identities = 198/377 (52%), Positives = 257/377 (68%), Gaps = 3/377 (0%) Query: 41 LQEKGSPMKTSSYANQRTGNISMMTAACGIAVANVYLCQPLLPAISASFSITSEQAGRVA 100 + K +P +S + G ++++ AACGI V NVYLCQPLL I+ S + ++ AG VA Sbjct: 1 MNTKNTPSTAASQPGR--GTLALLAAACGITVGNVYLCQPLLDQIAVSLGVPAQTAGLVA 58 Query: 101 TAAQIGYALGILLVVPLADRVSVTKLIRVLLALSAFFLLSAAAAPNIEILTLASLAITVA 160 AQ+GYALGILLV+PLAD ++ KL R LLAL+A FLL+AA +P +L AS+A+T Sbjct: 59 VGAQVGYALGILLVLPLADVIASRKLARTLLALTALFLLAAALSPTTPLLIAASVALTAT 118 Query: 161 TVIPQILIPIAVSTSSPEKAGSVVGTLQSGLIAGILLSRTFSGLIAQYSGSWRFSYVAAA 220 TVIPQILIPI ++PE G ++GTLQ+GLI GILLSRT SG +AQ + +WR Y+ AA Sbjct: 119 TVIPQILIPIISGLAAPEHRGRIIGTLQTGLILGILLSRTVSGAVAQATDTWRSPYLLAA 178 Query: 221 ACTVVLLLTLPRALPKRELPPVNISYFALIGSLPGLVSKWTHLRLSALLGALVFGAFSAF 280 T +LLL +PR +P RE + Y + + ++ LRLS LG LVFGAFSAF Sbjct: 179 ILTGLLLLIVPRLIPDRERGATHTGY-LNLLRSLPPLLRYRPLRLSMTLGFLVFGAFSAF 237 Query: 281 WATLAFHLAEQPFGYGSAEAGLFGLWGAAGALIAPLFGKIADRHGPSILNVISITAAALA 340 WATLAF LA FG G A AGLFGL+GA GA++AP+ G+++DR G +N++S+ A A+A Sbjct: 238 WATLAFELASPAFGLGPAAAGLFGLYGAPGAILAPMAGRLSDRIGSFKVNLLSLAAVAVA 297 Query: 341 FACFAGYGNIYVAAIVIGVNFLDFANQAGQIANQTRIFKLDPTARARLNTVYMVATFGGG 400 FA G I + A+V+ VN LDF Q+GQIANQTRIF L RAR+NT+YMVATFGGG Sbjct: 298 FALGGWVGGISLLALVVAVNLLDFGLQSGQIANQTRIFALGNDIRARVNTLYMVATFGGG 357 Query: 401 AVGSTLGSYAWSKHQWS 417 A GS G+ AWS W+ Sbjct: 358 AAGSFAGTLAWSLGGWT 374 >ref|ZP_06975051.1| major facilitator superfamily MFS_1 [Ktedonobacter racemifer DSM 44963] gb|EFH79708.1| major facilitator superfamily MFS_1 [Ktedonobacter racemifer DSM 44963] Length = 398 Score = 263 bits (671), Expect = 5e-68, Method: Composition-based stats. Identities = 171/375 (45%), Positives = 225/375 (60%), Gaps = 17/375 (4%) Query: 63 MMTAACGIAVANVYLCQPLLPAISASFSITSEQAGRVATAAQIGYALGILLVVPLADRVS 122 MM AC +++AN+Y QPLL + SF ++ Q G VAT Q G+A G+LLVVPL DR + Sbjct: 21 MMAVACTLSIANLYYVQPLLAEMGQSFRVSVNQIGFVATLMQFGFAAGLLLVVPLGDRYN 80 Query: 123 VTKLIRVLLALSAFFLLSAAAAPNIEILTLASLAITVATVIPQILIPIAVSTSSPEKAGS 182 LI +L + L+ A AP + +LTLA + ATV+PQI++P+A S ++P + G Sbjct: 81 RRTLIVSMLGVVTLALILVALAPTVTVLTLAGFLLGFATVVPQIIVPLAASLAAPFERGK 140 Query: 183 VVGTLQSGLIAGILLSRTFSGLIAQYSGSWRFSYVAAAACTVVLLLTLPRALPKRELPPV 242 V+GT+ SGL+ GILL+RTFSGLI Y G WR Y +AA +VL L L LPK + Sbjct: 141 VIGTVMSGLLIGILLARTFSGLIGAYLG-WRAVYWSAAVIMIVLALALRFLLPKDQ-QRS 198 Query: 243 NISYFALIGSLPGLVSKWTHLRLSALLGALVFGAFSAFWATLAFHLAEQPFGYGSAEAGL 302 +SY L+ SL L+ LR + + AL FGAFSAFW TL F L P+ YGS AGL Sbjct: 199 TMSYGQLLRSLGSLIRTEPILREAGVFYALSFGAFSAFWVTLTFMLQSPPYHYGSEVAGL 258 Query: 303 FGLWGAAGALIAPLFGKIADRHGPSILNVISITAAAL-----AFAC--FAGYGNIYVAAI 355 FGL G GA A G++ADR LN+ +T AL +F C GY ++ + Sbjct: 259 FGLVGVVGAFAASFVGRLADR-----LNIRHVTGLALLIVLCSFICMWLTGY---WLWGL 310 Query: 356 VIGVNFLDFANQAGQIANQTRIFKLDPTARARLNTVYMVATFGGGAVGSTLGSYAWSKHQ 415 ++GV LD Q I+NQTR++ LDP AR RLNTVYMV F GGA+GS LG++ WS Sbjct: 311 IVGVILLDLGVQGTHISNQTRVYSLDPEARNRLNTVYMVTGFIGGALGSYLGTWGWSLAG 370 Query: 416 WSGVWMTGFALIGGA 430 W+GV G L+ A Sbjct: 371 WNGVCSVGCILLVAA 385 >ref|YP_430703.1| major facilitator transporter [Moorella thermoacetica ATCC 39073] gb|ABC20160.1| Major facilitator superfamily MFS_1 [Moorella thermoacetica ATCC 39073] Length = 403 Score = 254 bits (649), Expect = 2e-65, Method: Composition-based stats. Identities = 161/377 (42%), Positives = 225/377 (59%), Gaps = 5/377 (1%) Query: 61 ISMMTAACGIAVANVYLCQPLLPAISASFSITSEQAGRVATAAQIGYALGILLVVPLADR 120 I +M CG++VAN+Y QPL IS +F ++ AG A Q+GYA G+LL VPL D Sbjct: 17 ILLMAVVCGVSVANLYYIQPLEGQISTTFHVSQSAAGIAAMLTQVGYAFGLLLFVPLGDM 76 Query: 121 VSVTKLIRVLLALSAFFLLSAAAAPNIEILTLASLAITVATVIPQILIPIAVSTSSPEKA 180 LI +L L A LL+A +P +L +A A+ + T++PQ+++P A S PE+ Sbjct: 77 CERRSLILHMLLLVAISLLTAGLSPCYPVLLIAMFAVGITTIVPQLIVPYAAHLSRPEEQ 136 Query: 181 GSVVGTLQSGLIAGILLSRTFSGLIAQYSGSWRFSYVAAAACTVVLLLTLPRALPKRELP 240 G ++G + SGL+ GILLSRTFSGL+ + +WR Y+ AA ++LL+ + P+ + P Sbjct: 137 GEIIGYVMSGLLIGILLSRTFSGLVGA-ALNWRAVYLFAAGFIIILLVLIRCFFPESQ-P 194 Query: 241 PVNISYFALIGSLPGLVSKWTHLRLSALLGALVFGAFSAFWATLAFHLAEQPFGYGSAEA 300 ISY L+ S+PGLV + LR +AL G +FG+FSAFW +L F L + + EA Sbjct: 195 SSKISYQELLKSIPGLVKRERPLREAALNGFFMFGSFSAFWTSLIFLLETPIYRMSTREA 254 Query: 301 GLFGLWGAAGALIAPLFGKIADRHGPSILNVISITAAALAFACFAGYG-NIYVAAIVIGV 359 GLFGL G AGAL APL GK AD P I + + LA+ CF+ +G NI+ ++IGV Sbjct: 255 GLFGLAGVAGALAAPLIGKAADTKSPRFTVGIGVILSTLAYLCFSLFGYNIW--GLIIGV 312 Query: 360 NFLDFANQAGQIANQTRIFKLDPTARARLNTVYMVATFGGGAVGSTLGSYAWSKHQWSGV 419 LD NQ GQ++NQ R+ L + R+R NTV+M + F GGA GS LG++ W + W GV Sbjct: 313 IVLDLGNQCGQVSNQARVQALGDSTRSRNNTVFMFSYFIGGAAGSFLGTFCWQHYGWYGV 372 Query: 420 WMTGFALIGGAAFLLFI 436 M G A A F+ Sbjct: 373 CMVGLAFQFAALITHFL 389 >ref|YP_002471090.1| hypothetical protein CKR_0625 [Clostridium kluyveri NBRC 12016] dbj|BAH05676.1| hypothetical protein [Clostridium kluyveri NBRC 12016] Length = 398 Score = 249 bits (635), Expect = 8e-64, Method: Composition-based stats. Identities = 151/365 (41%), Positives = 220/365 (60%), Gaps = 3/365 (0%) Query: 63 MMTAACGIAVANVYLCQPLLPAISASFSITSEQAGRVATAAQIGYALGILLVVPLADRVS 122 +M CG+ VAN+Y QPLL +S SF+++++ G V+T QIGY +G+ ++PL D + Sbjct: 23 IMAIICGVTVANLYYNQPLLGELSRSFNVSTKDIGLVSTFTQIGYGIGMFFIIPLGDIIE 82 Query: 123 VTKLIRVLLALSAFFLLSAAAAPNIEILTLASLAITVATVIPQILIPIAVSTSSPEKAGS 182 +I LL SA LL A+ +I+ + + S + TVIPQ+++P A S PE+ G Sbjct: 83 RKGMILKLLLASAISLLLFASCKDIKAVIVCSFLVGFTTVIPQLIVPFAAELSLPEERGR 142 Query: 183 VVGTLQSGLIAGILLSRTFSGLIAQYSGSWRFSYVAAAACTVVLLLTLPRALPKRELPPV 242 V+GT+ SGL+AGILL+RT SG I + G WR Y AA ++L + + R LPK E P Sbjct: 143 VLGTVLSGLLAGILLARTISGFIGTFLG-WRSMYYIAAVLMIILYIAVVRFLPKSE-PLS 200 Query: 243 NISYFALIGSLPGLVSKWTHLRLSALLGALVFGAFSAFWATLAFHLAEQPFGYGSAEAGL 302 SY ++ SL ++ K + LR ++L GA++FGAFS FW T+ F L + GS AGL Sbjct: 201 RESYKNILISLKDIIKKESLLREASLSGAMMFGAFSIFWTTIIFFLESPSYNMGSKTAGL 260 Query: 303 FGLWGAAGALIAPLFGKIADRHGPSILNVISITAAALAFACFAGYGNIYVAAIVIGVNFL 362 F L G +G + AP+ G +D+ G ISI AA F F +G+ V I+ + Sbjct: 261 FSLIGISGVVAAPIIGSFSDKKGTKFTVGISIFFAASGFVVFLIFGHNIVGLIIGLILLD 320 Query: 363 DFANQAGQIANQTRIFKLDPTARARLNTVYMVATFGGGAVGSTLGSYAWSKHQWSGVWMT 422 Q+GQ++NQTR+ L P AR RLNTV+MVA F GGA+GS +G+Y+W+ + W GV ++ Sbjct: 321 -LGTQSGQVSNQTRVHSLLPEARNRLNTVFMVAYFIGGALGSFIGTYSWNSYGWMGVCIS 379 Query: 423 GFALI 427 G + Sbjct: 380 GLTFL 384