BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.1980.p (432 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_004230820.1| major facilitator superfamily protein [Burkh... 603 e-170 ref|YP_003592845.1| major facilitator superfamily protein [Caulo... 498 e-138 ref|YP_003487179.1| hypothetical protein SCAB_14651 [Streptomyce... 261 2e-67 ref|ZP_08867931.1| major facilitator superfamily MFS_1 [Azospiri... 244 2e-62 ref|YP_004230844.1| major facilitator superfamily protein [Burkh... 188 2e-45 >ref|YP_004230820.1| major facilitator superfamily protein [Burkholderia sp. CCGE1001] gb|ADX57760.1| major facilitator superfamily MFS_1 [Burkholderia sp. CCGE1001] Length = 423 Score = 603 bits (1556), Expect = e-170, Method: Composition-based stats. Identities = 381/423 (90%), Positives = 398/423 (94%) Query: 10 MKHKAVPITTAAGLPLASDPPARTEMSREGIVVAIACSICMGLSFAPVFMGTFPLFLEPV 69 MKH VPI TA GLPLAS+ ARTEMSRE IVVAIACSICMGLSFAPVFMGTFPLFLEPV Sbjct: 1 MKHTVVPIPTAEGLPLASERSARTEMSREAIVVAIACSICMGLSFAPVFMGTFPLFLEPV 60 Query: 70 SRQFHWSAATFPQAMLISGLTGALSGPLVGRLIDTMGVRKVLLPGLLAWAAILAAMSLLN 129 SRQF WSAA FPQAMLISGLTGALSGPLVGRLIDTMGVRKVLLPGLLAWAA LAAMSLLN Sbjct: 61 SRQFQWSAAIFPQAMLISGLTGALSGPLVGRLIDTMGVRKVLLPGLLAWAATLAAMSLLN 120 Query: 130 GSIILLYVISALMGPLAATCGPVALAKVVSSWFDRSRGIALTAVLGGSVAIFTSLMLVLT 189 GSII+LYV+SALMGPLAATCGPVALAKVVSSWFDRSRGIALTAVLGGSVAIFTSLMLVL Sbjct: 121 GSIIVLYVVSALMGPLAATCGPVALAKVVSSWFDRSRGIALTAVLGGSVAIFTSLMLVLA 180 Query: 190 RVLILDIGWQGAYLALAGLIVAIAMPVSYFFLKEAPQSSPSVIMGKPDIASMDLKPMTAF 249 RVL+ +IGW+G YLALAGLIV IA+P+SYFFLKEAPQSSP ++ GKP +D+KPM AF Sbjct: 181 RVLMFNIGWRGTYLALAGLIVLIALPISYFFLKEAPQSSPPLMEGKPGPVGLDVKPMAAF 240 Query: 250 TSRAFWTVILASTLVCASCSGLSSHFVPWAGERGVSSATATFALTLYSLAGPFSSLLAGG 309 TSRAFWTVILASTLVCASCSGLSSHF+PW GERGVSSATATFALTLYSLAGPFSSL+AGG Sbjct: 241 TSRAFWTVILASTLVCASCSGLSSHFLPWGGERGVSSATATFALTLYSLAGPFSSLIAGG 300 Query: 310 AADRLPRPGMLAIVFALPLLGFLAMLTSQEWAVVIGLALMGSGFAAVAGLLPFFTSRYFG 369 AADRLPRPGMLAIVFALPLLGFLAMLTS EWAVVIGLALMG+GFAAVAGLLPFFTSRYFG Sbjct: 301 AADRLPRPGMLAIVFALPLLGFLAMLTSHEWAVVIGLALMGAGFAAVAGLLPFFTSRYFG 360 Query: 370 LANASTIFGVAVGLTTLSLGVGPVVLGILRDKWGAYTPASPVLAGALSLAIILALTLPRY 429 LANASTIFGVAVGLTTLSLGVGPVVLGILRDKWGAYTPASPV+AGALSLA+IL LTLPRY Sbjct: 361 LANASTIFGVAVGLTTLSLGVGPVVLGILRDKWGAYTPASPVVAGALSLAVILTLTLPRY 420 Query: 430 PRR 432 P R Sbjct: 421 PGR 423 >ref|YP_003592845.1| major facilitator superfamily protein [Caulobacter segnis ATCC 21756] gb|ADG10227.1| major facilitator superfamily MFS_1 [Caulobacter segnis ATCC 21756] Length = 435 Score = 498 bits (1281), Expect = e-138, Method: Composition-based stats. Identities = 302/427 (70%), Positives = 354/427 (82%) Query: 5 LEEHLMKHKAVPITTAAGLPLASDPPARTEMSREGIVVAIACSICMGLSFAPVFMGTFPL 64 +E M + PI G + + P R+E++R+ + VA+AC+ICMG FAP+F GTFP Sbjct: 1 MEGQRMTGTSGPIPADEGPGIIAAEPVRSEVTRQAVAVAVACAICMGFGFAPIFFGTFPQ 60 Query: 65 FLEPVSRQFHWSAATFPQAMLISGLTGALSGPLVGRLIDTMGVRKVLLPGLLAWAAILAA 124 FLEPVSRQF WSAA FPQAMLISG+TGALSGPLVGRLID +GVRKVLLPGL+AWAA L A Sbjct: 61 FLEPVSRQFQWSAAIFPQAMLISGVTGALSGPLVGRLIDKLGVRKVLLPGLVAWAATLVA 120 Query: 125 MSLLNGSIILLYVISALMGPLAATCGPVALAKVVSSWFDRSRGIALTAVLGGSVAIFTSL 184 MS LNGS+++LYV+SALMGPLAATCGPV LAKVVSSWFDRSRG+AL+A+LGGSVAIFT+L Sbjct: 121 MSFLNGSLVMLYVVSALMGPLAATCGPVGLAKVVSSWFDRSRGVALSAILGGSVAIFTAL 180 Query: 185 MLVLTRVLILDIGWQGAYLALAGLIVAIAMPVSYFFLKEAPQSSPSVIMGKPDIASMDLK 244 MLV+ R LI++IGW+ YL LAG IV IA+PVSYF L+EAP S + P A +K Sbjct: 181 MLVVARALIIEIGWRETYLVLAGSIVLIALPVSYFLLREAPHSVSPSMAMMPGSAVEGVK 240 Query: 245 PMTAFTSRAFWTVILASTLVCASCSGLSSHFVPWAGERGVSSATATFALTLYSLAGPFSS 304 P AFTSRAFWTV+L+STLVCASCSG+ SHFVPW+ ER VS ATFALTLYSL GPF++ Sbjct: 241 PTAAFTSRAFWTVMLSSTLVCASCSGVVSHFVPWSAERAVSPGIATFALTLYSLVGPFAA 300 Query: 305 LLAGGAADRLPRPGMLAIVFALPLLGFLAMLTSQEWAVVIGLALMGSGFAAVAGLLPFFT 364 L+AG AADRL RPGMLAIVF +PL+GFLAMLTSQ+WAV+IGL +MG+GFAAVAGLLPFFT Sbjct: 301 LIAGAAADRLRRPGMLAIVFMVPLIGFLAMLTSQDWAVIIGLTMMGAGFAAVAGLLPFFT 360 Query: 365 SRYFGLANASTIFGVAVGLTTLSLGVGPVVLGILRDKWGAYTPASPVLAGALSLAIILAL 424 SRYFGLANASTIFGVA+GLTTLSLG+GPV+LG+LRDKWGAY P SP++ GAL L +LA Sbjct: 361 SRYFGLANASTIFGVAIGLTTLSLGIGPVLLGVLRDKWGAYAPGSPIVVGALILGAVLAA 420 Query: 425 TLPRYPR 431 + P YPR Sbjct: 421 SFPPYPR 427 >ref|YP_003487179.1| hypothetical protein SCAB_14651 [Streptomyces scabiei 87.22] emb|CBG68613.1| putative transmembrane protein [Streptomyces scabiei 87.22] Length = 550 Score = 261 bits (666), Expect = 2e-67, Method: Composition-based stats. Identities = 163/416 (39%), Positives = 240/416 (57%), Gaps = 10/416 (2%) Query: 14 AVPITTAAGLPLASDPPARTEMSREGIVVAIACSICMGLSFAPVFMGTFPLFLEPVSRQF 73 VP++T A P +R V AI C IC+G P+F+GTFP+FL+PVS +F Sbjct: 126 GVPVSTPAS-------PKEGRNARTAAVAAIGCGICIGFGIIPLFIGTFPVFLKPVSDEF 178 Query: 74 HWSAATFPQAMLISGLTGALSGPLVGRLIDTMGVRKVLLPGLLAWAAILAAMSLLNGSII 133 WS + +PQA L+ GL GA+ GP VGRLID GVR+ L GL+ W A ++SL+ G Sbjct: 179 GWSLSLYPQASLLVGLAGAVVGPFVGRLIDRYGVRRTLPFGLVLWCAGFFSLSLIGGQPA 238 Query: 134 LLYVISALMGPLAATCGPVALAKVVSSWFDRSRGIALTAVLGGSVAIFTSLMLVLTRVLI 193 +Y+++ LM A GP++ AKV+S WFD++RG+AL VL G AI T++ + ++ LI Sbjct: 239 AMYLVALLMALGATIAGPISYAKVISGWFDKNRGLALGLVLSGVPAIATAVAVPVSAGLI 298 Query: 194 LDIGWQGAYLALAGLIVAIAMPVSYFFLKEAPQSSPSVIMGKPDIASMDLKPMTAFTSRA 253 GW+ AY LA + A+ +PVS ++EAP + G L A SRA Sbjct: 299 EADGWRAAYRMLAVVAAAVTIPVSILLIREAPARHGA---GPALSRDEGLTGSQALRSRA 355 Query: 254 FWTVILASTLVCASCSGLSSHFVPWAGERGVSSATATFALTLYSLAGPFSSLLAGGAADR 313 F VI ++ LV A +++H V W +GVS TATFAL+LYSL GP L+ G DR Sbjct: 356 FLMVIGSNCLVIAGLVAVTNHIVAWTTGQGVSPDTATFALSLYSLVGPVGPLVGGFVMDR 415 Query: 314 LPRPGMLAIVFALPLLGFLAMLTSQEWAVVIGLALMGSGFAAVAGLLPFFTSRYFGLANA 373 +P P ++A L +G + ++ G+AL+G ++++ GL+P +RY G+ + Sbjct: 416 VPSPKIVAAFLLLSPIGMALLFLGGTPGLLTGMALLGLTYSSITGLVPLLVTRYCGMKAS 475 Query: 374 STIFGVAVGLTTLSLGVGPVVLGILRDKWGAYTPASPVLAGALSLAIILALTLPRY 429 S I G A+ T+S+G+GPV+LG+ D G Y ++AG +++ I +L LP Y Sbjct: 476 SEILGGALAALTISMGIGPVLLGLGHDLAGGYAVPMAIVAGVITVGFIGSLFLPTY 531 >ref|ZP_08867931.1| major facilitator superfamily MFS_1 [Azospirillum amazonense Y2] gb|EGY02486.1| major facilitator superfamily MFS_1 [Azospirillum amazonense Y2] Length = 416 Score = 244 bits (623), Expect = 2e-62, Method: Composition-based stats. Identities = 153/398 (38%), Positives = 229/398 (57%), Gaps = 3/398 (0%) Query: 32 RTEMSREGIVVAIACSICMGLSFAPVFMGTFPLFLEPVSRQFHWSAATFPQAMLISGLTG 91 + +S + AI C+IC+ LS P+FMG FP+FLEPVS +F W + FPQA +I GLT Sbjct: 14 KRSLSAPALTAAIGCAICLALSLIPLFMGIFPVFLEPVSTEFGWGRSLFPQAPMIVGLTA 73 Query: 92 ALSGPLVGRLIDTMGVRKVLLPGLLAWAAILAAMSLLNGSIILLYVISALMGPLAATCGP 151 A++GP +GR++D GVR +L G W L ++SL++GS+ LY+++ ++G GP Sbjct: 74 AVAGPFIGRVLDRKGVRGILPIGFAIWYIGLFSLSLMDGSVAALYLVAVVIGIGGTIAGP 133 Query: 152 VALAKVVSSWFDRSRGIALTAVLGGSVAIFTSLMLVLTRVLILDIGWQGAYLALAGLIVA 211 +A AK+++ WFDR+RG+AL V+ G AI T++ + + + LI GW+ AY LA + Sbjct: 134 IAFAKIINGWFDRNRGLALGLVMSGVPAIATAIAIQVAQGLIDLQGWRVAYRILAIVAAV 193 Query: 212 IAMPVSYFFLKEAPQSSPSVIMGKPDIASMDLKPMTAFTSRAFWTVILASTLVCASCSGL 271 IA+P + ++EA G I + L AF SR F I AS + + G+ Sbjct: 194 IAIPTALLLVREAQHRDAP---GHARIIADGLTGSEAFRSRDFLLTIGASCIAVGALMGV 250 Query: 272 SSHFVPWAGERGVSSATATFALTLYSLAGPFSSLLAGGAADRLPRPGMLAIVFALPLLGF 331 ++HF+ W ERGV AT AL+L+SLAGP ++ G DR+ P ++A FA+ +G Sbjct: 251 TNHFLGWMTERGVDKDMATLALSLFSLAGPLGPIVGGFLVDRVSSPKVVASFFAMGPMGM 310 Query: 332 LAMLTSQEWAVVIGLALMGSGFAAVAGLLPFFTSRYFGLANASTIFGVAVGLTTLSLGVG 391 +L AVV G+ G GF+++ GL P+ SRYFG+ S I V T+ +GVG Sbjct: 311 GLLLFGGNAAVVPGMVFFGLGFSSINGLAPYLISRYFGMKAGSEILAVTFAALTIGMGVG 370 Query: 392 PVVLGILRDKWGAYTPASPVLAGALSLAIILALTLPRY 429 PV++G+ D +G Y + GA+ +I AL Y Sbjct: 371 PVLIGLGHDIYGGYGQTMAMAGGAMLFTLIAALFFQPY 408 >ref|YP_004230844.1| major facilitator superfamily protein [Burkholderia sp. CCGE1001] gb|ADX57784.1| major facilitator superfamily MFS_1 [Burkholderia sp. CCGE1001] Length = 415 Score = 188 bits (477), Expect = 2e-45, Method: Composition-based stats. Identities = 133/388 (34%), Positives = 212/388 (54%), Gaps = 5/388 (1%) Query: 42 VAIACSICMGLSFAPVFMGTFPLFLEPVSRQFHWSAATFPQAMLISGLTGALSGPLVGRL 101 V +A +CM L + +FMG+FP+FL PVSR+ W A + Q + IS + AL P GRL Sbjct: 9 VTVAAVLCMALDMSAIFMGSFPVFLGPVSREMGWGHAVYSQVITISSVCAALLMPFAGRL 68 Query: 102 IDTMGVRKVLLPGLLAWAAILAAMSLLNGSIILLYVISALMGPLAATCGPVALAKVVSSW 161 ID +GVR + GL + +S + G+ + + +G A+ GP + +VSSW Sbjct: 69 IDRIGVRWPVAIGLTLIGLSMVLLSFMRGTDTFFWAAALSLGIGASLAGPPSFVALVSSW 128 Query: 162 FDRSRGIALTAVLGGSVAIFTSLMLVLTRVLILDIGWQGAYLALAGLIVAIAMPVSYFFL 221 +DR R +AL+ VL + +++ + + LI+ +GW+ Y LAG+++ + + S L Sbjct: 129 YDRHRALALSVVLSVTPMCAQAIVAPVVQRLIMGVGWRETYQVLAGIVLFVGLAASLTSL 188 Query: 222 KEAPQSSPSVIMGKPDIASMDLKPMTAFTSRAFWTVILASTLVCASCSGLSSHFVPWAGE 281 + S+PS I KP A A T+ FW ++ A+ L AS G S H V W Sbjct: 189 R----SNPSGICMKPLPAVTRQVSSHAATTGTFWLLVAANCLSSASLIGFSVHTVGWLTS 244 Query: 282 RGVSSATATFALTLYSLAGPFSSLLAGGAADRLPRPGMLAIVFALPLLGFLAMLTSQEWA 341 R VS A+ +++ L+G +LL+ DR ++ + F++PLLG M + Sbjct: 245 RSVSPEGASAVMSVLFLSGVAGALLSASLVDRTSSLRIVQLFFSIPLLGLAIMAATTSLP 304 Query: 342 VV-IGLALMGSGFAAVAGLLPFFTSRYFGLANASTIFGVAVGLTTLSLGVGPVVLGILRD 400 VV +G L+G+G +A + L PF +RYFGL A+ IFG+ +GLT +++G+ PV++G+ D Sbjct: 305 VVMLGAVLLGAGMSATSALTPFLLTRYFGLKGAAEIFGILLGLTMIAIGLAPVLIGLGID 364 Query: 401 KWGAYTPASPVLAGALSLAIILALTLPR 428 + G YT +AGA+ +A + L R Sbjct: 365 RTGGYTIPLFFVAGAVVVATLCIDLLDR 392