BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.1968.p (356 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_08275620.1| Poly(3-hydroxybutyrate) depolymerase [Oxaloba... 242 5e-62 ref|YP_001860567.1| PHB depolymerase family esterase [Burkholder... 224 2e-56 ref|YP_001584846.1| PHB depolymerase family esterase [Burkholder... 216 4e-54 ref|ZP_03585303.1| esterase, PHB depolymerase family [Burkholder... 213 4e-53 ref|ZP_03570127.1| esterase, PHB depolymerase family [Burkholder... 213 5e-53 >ref|ZP_08275620.1| Poly(3-hydroxybutyrate) depolymerase [Oxalobacteraceae bacterium IMCC9480] gb|EGF30913.1| Poly(3-hydroxybutyrate) depolymerase [Oxalobacteraceae bacterium IMCC9480] Length = 268 Score = 242 bits (618), Expect = 5e-62, Method: Composition-based stats. Identities = 134/268 (50%), Positives = 172/268 (64%), Gaps = 12/268 (4%) Query: 89 IVMLHGCQQNAADFAQGTRMNAHAAKHGFAVLYPEQSLRAHPHRCWRWYAQDAQKGEGDV 148 +VMLHGC+Q A DFAQGTRMNA AA GF VLYP+QS RA +RCW W+ + Q GEG++ Sbjct: 1 MVMLHGCEQGAVDFAQGTRMNALAASKGFGVLYPQQSTRAQRNRCWPWFRNEVQAGEGEI 60 Query: 149 ALVASLIQEILKTHPIDPQRIYVCGISAGAALAHLLALTHPELIAAVALHAAPVSGVCSN 208 L+A++I+ ++ DP+R+YV G+SAGAA+AH+LAL HP+LIAAV LH+APV G S Sbjct: 61 LLIAAIIRRVVARQGFDPERVYVAGLSAGAAMAHILALRHPQLIAAVGLHSAPVFGAAST 120 Query: 209 AAGAYSVMQHGSLRAAAAAKRSLSR-----FPRTGALPAILIGGTDDKIVRPVNQRQLLE 263 A GAY VMQ G L AA ++++ FP A+PAILI G D +VRP+N QL + Sbjct: 121 ALGAYGVMQRGGLTRVGAAIQAVTDTGPVPFP---AMPAILIQGQQDSVVRPINFHQLEQ 177 Query: 264 QFLLLNHTQSLTEQPPRETPYGRTSKKQPRRRIVATRDYVRGRKLIIRSVLIEGLGHAWS 323 QF LN LT Q +E Y + T DY G+K ++R I L HAWS Sbjct: 178 QFCELN---GLTAQQRQEPTYRAAGRSARAAHGFNTLDYRVGKKTLLRLCEIHQLKHAWS 234 Query: 324 GGDTTLSYNA-AGPDASRLVVDFFSRHR 350 GGD L +N GPDASR++ FF+ HR Sbjct: 235 GGDPALPFNERKGPDASRMMWTFFAPHR 262 >ref|YP_001860567.1| PHB depolymerase family esterase [Burkholderia phymatum STM815] gb|ACC73521.1| esterase, PHB depolymerase family [Burkholderia phymatum STM815] Length = 398 Score = 224 bits (571), Expect = 2e-56, Method: Composition-based stats. Identities = 149/384 (38%), Positives = 203/384 (52%), Gaps = 43/384 (11%) Query: 2 KKLSRLWLSAMKKAGKVQRKQFLDFLS--PASPRSKTSSIRKP----------------- 42 K L+++WL +K+ +Q + L P R+ T S +P Sbjct: 3 KSLTKIWLGGLKRLFAIQSEHVQATLKRKPKPKRTSTRSATRPAQTVAKPSAKIRPTVTR 62 Query: 43 ----------KTAPRGASASTSEVTVTSAVHHVPATTHSL----QYRLCLPKPTP-SELP 87 + PR A+ ++ + T + H PA SL QY L LP P P Sbjct: 63 DVPQRGTRESRVRPRAAAWASG--SWTRSFHSAPAAPGSLVNHLQYGLYLPAGKPVKRAP 120 Query: 88 LIVMLHGCQQNAADFAQGTRMNAHAAKHGFAVLYPEQSLRAHPHRCWRWYAQDAQKGEGD 147 LIVMLHGC Q+ +FA+GTRMN A ++GFAV+YPEQS AH HRCW WY + G + Sbjct: 121 LIVMLHGCTQSIDEFAEGTRMNLLADRYGFAVVYPEQSKHAHAHRCWHWYDDTDRAGRAE 180 Query: 148 VALVASLIQEILKTHPIDPQRIYVCGISAGAALAHLLALTHPELIAAVALHAAPVSGVCS 207 V SL+ +++TH D +RIYV GISAGA L LLAL PE AAVALH+ P G Sbjct: 181 ARAVVSLVDALVETHGFDRERIYVAGISAGAGLTSLLALHFPEHFAAVALHSGPAFGEAH 240 Query: 208 NAAGAYSVMQHGSLRAAAAAKRSLSRFPRTGALPAILIGGTDDKIVRPVNQRQLLEQFLL 267 + A VM+ G+ + A +++ +PAI++ G D++V PVN QL EQFL Sbjct: 241 SGITAMDVMRRGTRQDPVALVDAVADVTAYPGMPAIILQGEADRVVAPVNAEQLTEQFLR 300 Query: 268 LNHTQSLTEQPPRETPYGRTSKKQPRRRIVATRDYVRGRKLIIRSVLIEGLGHAWSGGDT 327 LN + Q R+ K+ R+ V TRDY RG + I+R + GLGHAWSGGD Sbjct: 301 LNGI--VDAQGVRKV----GDVKEDRKATVTTRDYTRGGRRIVRLCRVHGLGHAWSGGDD 354 Query: 328 TLSYNAA-GPDASRLVVDFFSRHR 350 + ++++ GPDAS L+ DFF R Sbjct: 355 AVPFHSSKGPDASSLLWDFFRHQR 378 >ref|YP_001584846.1| PHB depolymerase family esterase [Burkholderia multivorans ATCC 17616] ref|YP_001948040.1| poly(3-hydroxybutyrate) depolymerase [Burkholderia multivorans ATCC 17616] gb|ABX18554.1| esterase, PHB depolymerase family [Burkholderia multivorans ATCC 17616] dbj|BAG45504.1| poly(3-hydroxybutyrate) depolymerase [Burkholderia multivorans ATCC 17616] Length = 369 Score = 216 bits (551), Expect = 4e-54, Method: Composition-based stats. Identities = 149/364 (40%), Positives = 197/364 (54%), Gaps = 22/364 (6%) Query: 2 KKLSRLWLSAMKK--------AGKVQRKQFLDFL-SPASPR-SKTSSIRKPKTAPRGASA 51 K L+++WL MK+ A + R+ D L + ASP S+ S R+ + PR A+ Sbjct: 3 KSLTKMWLGGMKRVLSPAAKRAARDARRLTRDVLDAAASPAASEVSMPRESRVRPRAAAW 62 Query: 52 STSEVTVTSAVHHVPAT---THSLQYRLCLPKPTPSE-LPLIVMLHGCQQNAADFAQGTR 107 + E T PA L Y L +P E +PL+VMLHGCQQ A FAQGTR Sbjct: 63 AAGEWT-RGEHPMAPALGRLVQQLAYALYVPPGHRREAMPLVVMLHGCQQTADAFAQGTR 121 Query: 108 MNAHAAKHGFAVLYPEQSLRAHPHRCWRWYAQDAQKGEGDVALVASLIQEILKTHPIDPQ 167 MN A KHGFAVLYPEQS+RAH H CW WY + G G+ VASL+ ++ H D Sbjct: 122 MNLLADKHGFAVLYPEQSMRAHSHGCWHWYEDTDRAGRGEADAVASLVDALVDEHGFDAS 181 Query: 168 RIYVCGISAGAALAHLLALTHPELIAAVALHAAPVSGVCSNAAGAYSVMQHGSLRAAAAA 227 R+YV G+SAGA LA LLAL HP+ AAVALH+ P G ++ A VM+ G AA Sbjct: 182 RVYVAGLSAGAGLASLLALHHPDRFAAVALHSGPALGEANSGISAMDVMRRGLRENPAAV 241 Query: 228 KRSLSRFPRTGALPAILIGGTDDKIVRPVNQRQLLEQFLLLNHTQSLTEQPPRETPYGRT 287 +L +PA+++ G D++V P N +L QF+ LN L + R Sbjct: 242 VDALVDADSYPGMPALIVQGDGDRVVAPKNADELAVQFMRLN---GLADSRGALRGGERV 298 Query: 288 SKKQPRRRIVATRDYVRGRKLIIRSVLIEGLGHAWSGGDTTLSYNAA-GPDASRLVVDFF 346 +Q RIV R R + ++R ++GL HAWSGGD + ++AA GPDAS +V FF Sbjct: 299 ETRQAGARIVDVR---RDGESVVRLCHVKGLDHAWSGGDEAVPFHAAVGPDASAMVWSFF 355 Query: 347 SRHR 350 HR Sbjct: 356 ETHR 359 >ref|ZP_03585303.1| esterase, PHB depolymerase family [Burkholderia multivorans CGD1] gb|EED99894.1| esterase, PHB depolymerase family [Burkholderia multivorans CGD1] Length = 357 Score = 213 bits (542), Expect = 4e-53, Method: Composition-based stats. Identities = 139/328 (42%), Positives = 181/328 (55%), Gaps = 14/328 (4%) Query: 28 SPASPRSKTSSIRKPKTAPRGASASTSEVTVTSAVHHVPAT---THSLQYRLCLPKPTPS 84 SPA+ S+ S R+ + PR A+ + E T PA L Y L +P Sbjct: 29 SPAA--SEVSMPRESRVRPRAAAWAAGEWT-RGEHPMAPALGRLVQQLAYALYVPPGHRR 85 Query: 85 E-LPLIVMLHGCQQNAADFAQGTRMNAHAAKHGFAVLYPEQSLRAHPHRCWRWYAQDAQK 143 E +PL+VMLHGCQQ A FAQGTRMN A KHGFAVLYPEQS+RAH H CW WY + Sbjct: 86 EAMPLVVMLHGCQQTADAFAQGTRMNLLADKHGFAVLYPEQSMRAHSHGCWHWYEDTDRA 145 Query: 144 GEGDVALVASLIQEILKTHPIDPQRIYVCGISAGAALAHLLALTHPELIAAVALHAAPVS 203 G G+ VASL+ ++ H D R+YV G+SAGA LA LLAL HP+ AAVALH+ P Sbjct: 146 GRGEADAVASLVDALVDEHGFDASRVYVAGLSAGAGLASLLALHHPDRFAAVALHSGPAL 205 Query: 204 GVCSNAAGAYSVMQHGSLRAAAAAKRSLSRFPRTGALPAILIGGTDDKIVRPVNQRQLLE 263 G ++ A VM+ G AA +L +PA+++ G D++V P N +L Sbjct: 206 GEANSGISAMDVMRRGLRENPAAVVDALVDADSYPGMPALIVQGDGDRVVAPKNADELAV 265 Query: 264 QFLLLNHTQSLTEQPPRETPYGRTSKKQPRRRIVATRDYVRGRKLIIRSVLIEGLGHAWS 323 QF+ LN L + R +Q RIV R R + ++R ++GL HAWS Sbjct: 266 QFMRLN---GLADSRGALRGGERVETRQAGARIVDVR---RDGESVVRLCHVKGLDHAWS 319 Query: 324 GGDTTLSYNAA-GPDASRLVVDFFSRHR 350 GGD + ++AA GPDAS +V FF HR Sbjct: 320 GGDEAVPFHAAVGPDASAMVWSFFETHR 347 >ref|ZP_03570127.1| esterase, PHB depolymerase family [Burkholderia multivorans CGD2M] ref|ZP_03576770.1| esterase, PHB depolymerase family [Burkholderia multivorans CGD2] gb|EEE08347.1| esterase, PHB depolymerase family [Burkholderia multivorans CGD2] gb|EEE16034.1| esterase, PHB depolymerase family [Burkholderia multivorans CGD2M] Length = 369 Score = 213 bits (541), Expect = 5e-53, Method: Composition-based stats. Identities = 148/364 (40%), Positives = 196/364 (53%), Gaps = 22/364 (6%) Query: 2 KKLSRLWLSAMKK--------AGKVQRKQFLDFL-SPASP-RSKTSSIRKPKTAPRGASA 51 K L+++WL MK+ A + R+ D L + ASP S+ S R+ + PR A+ Sbjct: 3 KSLTKMWLGGMKRVLSPAAKRAARDARRLTRDVLDAAASPVASEVSMPRESRVRPRAAAW 62 Query: 52 STSEVTVTSAVHHVPAT---THSLQYRLCLPKPTPSE-LPLIVMLHGCQQNAADFAQGTR 107 + E T PA L Y L +P E +PL+VMLHGCQQ A FAQGTR Sbjct: 63 AAGEWT-RGEHPMAPALGRLVQQLAYALYVPPGHRREAMPLVVMLHGCQQTADAFAQGTR 121 Query: 108 MNAHAAKHGFAVLYPEQSLRAHPHRCWRWYAQDAQKGEGDVALVASLIQEILKTHPIDPQ 167 MN A KHGFAVLYPEQS+RAH H CW WY + G G+ VASL+ ++ H D Sbjct: 122 MNLLADKHGFAVLYPEQSMRAHSHGCWHWYEDTDRAGRGEADAVASLVDALVDEHGFDAS 181 Query: 168 RIYVCGISAGAALAHLLALTHPELIAAVALHAAPVSGVCSNAAGAYSVMQHGSLRAAAAA 227 R+YV G+SAGA LA LLAL H + AAVALH+ P G ++ A VM+ G AA Sbjct: 182 RVYVAGLSAGAGLASLLALHHADRFAAVALHSGPALGEANSGISAMDVMRRGLRENPAAV 241 Query: 228 KRSLSRFPRTGALPAILIGGTDDKIVRPVNQRQLLEQFLLLNHTQSLTEQPPRETPYGRT 287 +L +PA+++ G D++V P N +L QF+ LN L + R Sbjct: 242 VDALIDADSYPGMPALILQGDGDRVVAPKNADELAVQFMRLN---GLADSRGALRGGERV 298 Query: 288 SKKQPRRRIVATRDYVRGRKLIIRSVLIEGLGHAWSGGDTTLSYNAA-GPDASRLVVDFF 346 +Q RIV R R + ++R ++GL HAWSGGD + ++AA GPDAS +V FF Sbjct: 299 ETRQAGARIVDVR---RDGESVVRLCHVKGLEHAWSGGDEAVPFHAAVGPDASAMVWSFF 355 Query: 347 SRHR 350 HR Sbjct: 356 ETHR 359