BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.1962.p (527 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_08869891.1| major facilitator superfamily protein [Azospi... 457 e-126 ref|YP_001260959.1| hypothetical protein Swit_0453 [Sphingomonas... 341 2e-91 ref|ZP_08388178.1| major Facilitator Superfamily protein [Sphing... 334 3e-89 ref|ZP_05032323.1| transporter, major facilitator family [Brevun... 327 3e-87 ref|ZP_07250713.1| major facilitator transporter [Pseudomonas sy... 324 3e-86 >ref|ZP_08869891.1| major facilitator superfamily protein [Azospirillum amazonense Y2] gb|EGY00517.1| major facilitator superfamily protein [Azospirillum amazonense Y2] Length = 484 Score = 457 bits (1176), Expect = e-126, Method: Composition-based stats. Identities = 287/484 (59%), Positives = 354/484 (73%), Gaps = 5/484 (1%) Query: 41 VLAGIGATFGNGMVNVNIASLAGGQGLYTAEASLLPAVYVGFNACANLILVKARAQFGIP 100 +L G+ AT GN +VNVN+ASL+G G+Y +AS L A+YV FNA ANL+LVKAR QFGIP Sbjct: 1 MLVGVAATLGNALVNVNLASLSGALGVYVDQASWLLALYVAFNAGANLMLVKARVQFGIP 60 Query: 101 QVTLTLLATYALAALLHLCVPGLAAAIVVRAACGLAAAALTTTTIYHLLQVFSLKARPLA 160 VT TLL YALA LL VPG AA ++RA G+ AA LTT TIY+LLQVF + RPLA Sbjct: 61 AVTCTLLIAYALAGLLQFVVPGFTAAAIIRAVSGMTAAGLTTMTIYNLLQVFPGRLRPLA 120 Query: 161 LVVGVSLPQFGPPLARLMSLDFLAESQWQGLLLTELCIALVLLGAMTLVPLPPSERSQAF 220 LV+G+ L Q G PLARL+ ++ LA WQGL L E+ IAL +L A+T +PLPPS+R+ AF Sbjct: 121 LVIGIGLTQLGTPLARLVPVEMLALHDWQGLHLMEIGIALAVLAAITALPLPPSDRTPAF 180 Query: 221 EGMDLVTISLLVPAGLLLSAVLGLGRILWWTDTPWLGLALAGALVLASAAWMIERQRARP 280 + +D I L++P +L+ VLG GR+LWW DTPWLG ALA A+ L + AW+IER+R RP Sbjct: 181 QPLDFAVIGLMLPCLVLVCGVLGQGRLLWWVDTPWLGWALAAAVPLFTLAWLIERRRDRP 240 Query: 281 LLQLRWMLTGDIVRFAMVAFLVRIALAEQTYGAVGLLSAGGLNNDQLHGLFLAVTAAMFL 340 L+++ W+ T D+ RFA A LVR++LAEQTYGAVGLL+AGGL NDQLH LF V AM Sbjct: 241 LIRVHWLTTLDMARFAAAALLVRVSLAEQTYGAVGLLTAGGLINDQLHLLFAIVALAMLG 300 Query: 341 GIIVAALTLSERNLPWQVAVAALCIALAAWLDSGSNSLTRPPQLMLSQSLVAFGTTLFVG 400 G + AALTLSER LP+QV A+L IA AWLDS + +L RPPQL LSQ+L+AFGTTLF+G Sbjct: 301 GTLTAALTLSERRLPYQVIAASLLIAAGAWLDSDATNLVRPPQLYLSQALIAFGTTLFIG 360 Query: 401 PTLVFGFLRMSARGGDHLVSFIVLFSVSQNIGGLAGSALLGSVQTIMAKVHAQVMSERLL 460 P LV GFLRM A+G D+LV+F+VLFS++QN+GGLAGSALLGS+Q + HAQ +SE L+ Sbjct: 361 PALVLGFLRMVAKGPDYLVTFMVLFSITQNVGGLAGSALLGSLQVASSHWHAQTLSEHLV 420 Query: 461 ASDPQTAARLAQ-GSGALLQSLMTEANTLAYNDVFRLVA----LAALAVAAYLACETWSR 515 ASDPQ ARLA G GAL Q+L EA LA+NDVF VA L AL V + T R Sbjct: 421 ASDPQVVARLAMGGGGALSQALGREAAVLAFNDVFLFVAGLALLNALYVGYLVLFNTLRR 480 Query: 516 RRAA 519 RR + Sbjct: 481 RRGS 484 >ref|YP_001260959.1| hypothetical protein Swit_0453 [Sphingomonas wittichii RW1] gb|ABQ66821.1| hypothetical protein Swit_0453 [Sphingomonas wittichii RW1] Length = 561 Score = 341 bits (874), Expect = 2e-91, Method: Composition-based stats. Identities = 212/521 (40%), Positives = 300/521 (57%), Gaps = 18/521 (3%) Query: 15 EAPAFPGSPYIATHAPGRRAAYAAIAVLAGIGATFGNGMVNVNIASLAGGQGLYTAEASL 74 E P PGSP H RR Y AI L G+ GN +V VN+ G GL + EA+ Sbjct: 23 ERPTLPGSPANPDHPSRRRMGYFAIGCLIGVTGGLGNALVTVNLNFAQGTLGLNSDEATW 82 Query: 75 LPAVYVGFNACANLILVKARAQFGIPQVTLTLLATYALAALLHLCVPGLAAAIVVRAACG 134 L A Y N ANL+LVK R QFG+ +LA YA+ AL HL V ++ VRAA G Sbjct: 83 LTAAYFMTNVTANLLLVKFRQQFGLQMFIRYMLAAYAVTALAHLLVHDFWTSVAVRAASG 142 Query: 135 LAAAALTTTTIYHLLQVFSLKARPLALVVGVSLPQFGPPLARLMSLDFLAESQWQGLLLT 194 +AA+ L+T T+ +L Q S R +++G+S+PQ PLAR++S L W L Sbjct: 143 IAASGLSTLTVLYLSQGMSAPKRLAGIMIGISIPQLATPLARVLSPGLLIWGDWHMLYWF 202 Query: 195 ELCIALVLLGAMTLVPLPPSERSQAFEGMDLVTISLLVPAGLLLSAVLGLGRILWWTDTP 254 EL + L +L A+ +PLPPSER AFE +D +T +LL P LL AVL GRI WW + P Sbjct: 203 ELGMTLAMLAAVMSLPLPPSERVHAFEKLDFLTFALLAPGLWLLVAVLSEGRIQWWAERP 262 Query: 255 WLGLALAGALVLASAAWMIERQRARPLLQLRWMLTGDIVRFAMVAFLVRIALAEQTYGAV 314 W+G +LAG++ L +AA ++E R PL+ RW+ T ++VR VA +RI L+EQ +G+V Sbjct: 263 WIGWSLAGSIALIAAALVVEHHRVNPLINTRWLGTREMVRLIAVASSIRILLSEQAFGSV 322 Query: 315 GLLSAGGLNNDQLHGLFLAVTAAMFLGIIVAALTLSERNLPWQVAVAALCIALAAWLDSG 374 GLL+ G+ NDQ+ L L + A GI A LT N+ + +A + IA+ +++D+ Sbjct: 323 GLLTTLGMLNDQMVTLNLVIIVASVAGIAAAVLTFRPANVARPLTIAVILIAIGSFMDAD 382 Query: 375 SNSLTRPPQLMLSQSLVAFGTTLFVGPTLVFGFLRMSARGGDHLVSFIVLFSVSQNIGGL 434 + +LTRP +SQ+++ F + LF+ +V G R G H +SFIVLFS+SQ+IGGL Sbjct: 383 TTNLTRPSSFYVSQAIIGFASLLFMAQAIVIGIARTLLAGTKHFISFIVLFSLSQSIGGL 442 Query: 435 AGSALLGSVQTIMAKVHAQVMSERLLASDPQTAARL------------------AQGSGA 476 GSALLG+ Q + K H+ + + ++ +DPQ AAR+ AQG+ Sbjct: 443 VGSALLGTFQVVREKYHSHELVQSIVMNDPQVAARIGAGARSLGGVIGDPVLQGAQGAAL 502 Query: 477 LLQSLMTEANTLAYNDVFRLVALAALAVAAYLACETWSRRR 517 L +++ EAN LAYNDVF L+ + A+A + S RR Sbjct: 503 LARTVTREANILAYNDVFLLIGVLAVATVIWSYGIRLSMRR 543 >ref|ZP_08388178.1| major Facilitator Superfamily protein [Sphingomonas sp. S17] gb|EGI55478.1| major Facilitator Superfamily protein [Sphingomonas sp. S17] Length = 548 Score = 334 bits (856), Expect = 3e-89, Method: Composition-based stats. Identities = 227/520 (43%), Positives = 303/520 (58%), Gaps = 18/520 (3%) Query: 12 AAPEAPAFPGSPYIATHAPGRRAAYAAIAVLAGIGATFGNGMVNVNIASLAGGQGLYTAE 71 A E P PGSP H RR AY IAVL G+ GN +V VN L G GL E Sbjct: 17 APHERPTIPGSPGNFDHPLRRRFAYGLIAVLIGLTGGLGNALVQVNTVQLQGALGLDPTE 76 Query: 72 ASLLPAVYVGFNACANLILVKARAQFGIPQVTLTLLATYALAALLHLCVPGLAAAIVVRA 131 + LP YV NA NL+L+K R QFG+ L + Y + A HL V AI VRA Sbjct: 77 IAWLPIAYVMTNASINLLLIKFRQQFGLRPFALLFVGLYTILAFAHLFVRDFGTAIAVRA 136 Query: 132 ACGLAAAALTTTTIYHLLQVFSLKARPLALVVGVSLPQFGPPLARLMSLDFLAESQWQGL 191 A G+AAAAL++ +Y+++Q K R A+V+G+ +PQ PLARL S + LA SQW+ L Sbjct: 137 ASGMAAAALSSLGLYYMMQALPAKWRLKAIVLGIGVPQCATPLARLFSPELLAMSQWRTL 196 Query: 192 LLTELCIALVLLGAMTLVPLPPSERSQAFEGMDLVTISLLVPAGLLLSAVLGLGRILWWT 251 + E +A + L A+ + LPP++R +AFE +D +T +L PA L AVLG GR++WWT Sbjct: 197 YMFEFGLAAISLAAIIALRLPPTQRLKAFEPLDFLTFALYAPAIGLFCAVLGQGRLVWWT 256 Query: 252 DTPWLGLALAGALVLASAAWMIERQRARPLLQLRWMLTGDIVRFAMVAFLVRIALAEQTY 311 W+G AL A+ L +AA IE RA PLL RW+ + DIVRF +V + RI L+EQ + Sbjct: 257 QAAWIGWALVIAIPLLAAALWIEHHRANPLLNTRWLGSLDIVRFTIVTLMARIVLSEQNF 316 Query: 312 GAVGLLSAGGLNNDQLHGLFLAVTAAMFLGIIVAALTLSERNLPWQVAVAALCIALAAWL 371 GAVGLL+ G NNDQ LF V A G++ +A+TL L + A +A+AA+ Sbjct: 317 GAVGLLTTLGQNNDQFRTLFTIVFIASVAGVVASAVTLDVTRLTHPIMFAIGLVAIAAFA 376 Query: 372 DSGSNSLTRPPQLMLSQSLVAFGTTLFVGPTLVFGFLRMSARGGDHLVSFIVLFSVSQNI 431 DS S +LTR PQL +Q+L+AF TT F+GP+L+FG R +G H++SFI LF + ++ Sbjct: 377 DSFSTNLTRAPQLYATQALIAFSTTFFLGPSLLFGMTRALQQGAGHVISFIALFGMLNSV 436 Query: 432 GGLAGSALLGSVQTIMAKVHAQVMSERLLASDPQTAARL------------------AQG 473 G L G+ALLG+ Q I K H+ + + + +DPQ R+ A+G Sbjct: 437 GSLGGTALLGTYQVIREKAHSAAIVQNIDPTDPQVTQRIQAGGARVSGVVSDPTLARAEG 496 Query: 474 SGALLQSLMTEANTLAYNDVFRLVALAALAVAAYLACETW 513 S L Q+ EAN AYNDVFRLVA A AV +LA TW Sbjct: 497 SALLSQAATREANIRAYNDVFRLVAALAAAVTMFLALLTW 536 >ref|ZP_05032323.1| transporter, major facilitator family [Brevundimonas sp. BAL3] gb|EDX79752.1| transporter, major facilitator family [Brevundimonas sp. BAL3] Length = 570 Score = 327 bits (839), Expect = 3e-87, Method: Composition-based stats. Identities = 212/505 (41%), Positives = 297/505 (58%), Gaps = 18/505 (3%) Query: 15 EAPAFPGSPYIATHAPGRRAAYAAIAVLAGIGATFGNGMVNVNIASLAGGQGLYTAEASL 74 E P PGSP H R YA VL + + GN V N+ SL G G+ EA+ Sbjct: 24 ERPTLPGSPATPDHTAWLRLQYALTGVLVALVGSLGNAAVTANLTSLQGSLGITITEAAW 83 Query: 75 LPAVYVGFNACANLILVKARAQFGIPQVTLTLLATYALAALLHLCVPGLAAAIVVRAACG 134 LP V+V NAC NLIL K R Q+G+ T L + + HL V + + VRA G Sbjct: 84 LPVVFVMTNACMNLILFKFRQQYGLRLFTQICLTAFVVTCAAHLAVDNYESTLFVRAVAG 143 Query: 135 LAAAALTTTTIYHLLQVFSLKARPLALVVGVSLPQFGPPLARLMSLDFLAESQWQGLLLT 194 +AA L+T +++Q F + R LV+G+ L F P+ARL+ L + W+ Sbjct: 144 MAAVGLSTLGFLYMIQAFPAQHRLKGLVIGIGLSSFAVPIARLIMPSLLEMNDWRAFYTF 203 Query: 195 ELCIALVLLGAMTLVPLPPSERSQAFEGMDLVTISLLVPAGLLLSAVLGLGRILWWTDTP 254 EL +AL+ A+ ++ LPPSER + FE +D VT +L P LL+AVLGLGRI+WWT+ Sbjct: 204 ELGMALLAWAAVQVLRLPPSERLKLFEPLDFVTFALFAPGVALLAAVLGLGRIVWWTEAA 263 Query: 255 WLGLALAGALVLASAAWMIERQRARPLLQLRWMLTGDIVRFAMVAFLVRIALAEQTYGAV 314 W+G ALAGA++L +AA MIE R PL+ +RW+ DI+R L+RI L+EQT GAV Sbjct: 264 WIGWALAGAILLLTAALMIEYDRRNPLINVRWLTGPDILRLFGAILLIRITLSEQTSGAV 323 Query: 315 GLLSAGGLNNDQLHGLFLAVTAAMFLGIIVAALTLSERNLPWQVAVAALCIALAAWLDSG 374 G L+ GL DQLHGLFL + AM G V+ALTL+ L +A+A IA+ A++DS Sbjct: 324 GFLTVVGLGPDQLHGLFLLILLAMIAGTAVSALTLNLTKLNKPIAIALGLIAVGAFIDSH 383 Query: 375 SNSLTRPPQLMLSQSLVAFGTTLFVGPTLVFGFLRMSARGGDHLVSFIVLFSVSQNIGGL 434 + LTRP QL +SQ+++AF + +FVGP L+ G ++ +G +++SFIV+FSV QN+G L Sbjct: 384 ATVLTRPAQLYVSQAMIAFASAMFVGPALLIGVGKVLQQGARNIISFIVMFSVLQNLGAL 443 Query: 435 AGSALLGSVQTIMAKVHAQVMSERLLASDPQTAARLAQGSGA------------------ 476 AGSAL+G++Q + K H+ +S + + +P+ RL Q SGA Sbjct: 444 AGSALIGTIQVMREKFHSNQLSADITSLNPEVVLRLRQLSGAYASTITDPALLNAEGVAM 503 Query: 477 LLQSLMTEANTLAYNDVFRLVALAA 501 L + + +AN LAYNDVF ++A AA Sbjct: 504 LTRQVTQQANILAYNDVFLIIAAAA 528 >ref|ZP_07250713.1| major facilitator transporter [Pseudomonas syringae pv. tomato K40] Length = 559 Score = 324 bits (830), Expect = 3e-86, Method: Composition-based stats. Identities = 221/526 (42%), Positives = 304/526 (57%), Gaps = 22/526 (4%) Query: 5 LPDRPAP---AAPEAPAFPGSPYIATHAPGRRAAYAAIAVLAGIGATFGNGMVNVNIASL 61 + D+ AP AA E P PGSP H+ RR A+A + V+ + GN +V N+ L Sbjct: 1 MTDKYAPRDWAAHERPTMPGSPSTPLHSDARRWAFALVGVIVALTGGLGNALVVANLPYL 60 Query: 62 AGGQGLYTAEASLLPAVYVGFNACANLILVKARAQFGIPQVTLTLLATYALAALLHLCVP 121 G G + + + LPA Y+ N NL+LVK R QFG+ T L YAL L HL V Sbjct: 61 QGALGATSQDIAWLPAAYIMTNVSMNLLLVKFRQQFGLRAFTELFLVLYALVTLAHLFVN 120 Query: 122 GLAAAIVVRAACGLAAAALTTTTIYHLLQVFSLKARPLALVVGVSLPQFGPPLARLMSLD 181 + +AI VRAA G+ AAL+T +Y+++Q F K R ALV+G+ Q PLARL+S D Sbjct: 121 DIDSAIAVRAAHGMVGAALSTLGLYYMIQAFPQKWRLKALVLGLGTAQLATPLARLVSED 180 Query: 182 FLAESQWQGLLLTELCIALVLLGAMTLVPLPPSERSQAFEGMDLVTISLLVPAGLLLSAV 241 L +QW+GL L EL +AL+ LG + L+ LPP +R +AFE +D +T LL LL AV Sbjct: 181 LLQIAQWRGLYLFELGMALLSLGCVLLLKLPPGDRFKAFEKLDFLTFGLLASGFALLCAV 240 Query: 242 LGLGRILWWTDTPWLGLALAGALVLASAAWMIERQRARPLLQLRWMLTGDIVRFAMVAFL 301 L LGRI WW + PW+G A A ++VL A IE R+ PLL RW+ +G I+R + L Sbjct: 241 LSLGRIEWWLEAPWIGAASAASIVLICAGLAIEHNRSNPLLITRWLGSGAILRLGLAVIL 300 Query: 302 VRIALAEQTYGAVGLLSAGGLNNDQLHGLFLAVTAAMFLGIIVAALTLSERNLPWQVAVA 361 RI L+EQ+ GAVG L A + + QLH L+L + G+ V+ALT++ +L + ++ Sbjct: 301 FRIVLSEQSVGAVGFLQALNMGSQQLHTLYLVMLLGSVAGLAVSALTINPAHLIKPLLIS 360 Query: 362 ALCIALAAWLDSGSNSLTRPPQLMLSQSLVAFGTTLFVGPTLVFGFLRMSARGGDHLVSF 421 +AL AW+DS S +LTR + LSQ L+AFG T F+GPTLV G + A ++VSF Sbjct: 361 LAMMALGAWMDSHSTNLTRQSNMYLSQFLLAFGGTFFLGPTLVLGISGVLA-NPRNMVSF 419 Query: 422 IVLFSVSQNIGGLAGSALLGSVQTIMAKVHAQVMSERLLASDPQTAARL----------- 470 VLF ++QNIGGL GSA LG+ Q + K H+ + E L DP +RL Sbjct: 420 SVLFGITQNIGGLIGSAALGTFQIVREKFHSSHIVEHLTLIDPLVQSRLQSYSASYSSVI 479 Query: 471 -------AQGSGALLQSLMTEANTLAYNDVFRLVALAALAVAAYLA 509 +QG A+ + EAN LAYNDVF L+AL A+ +++ Sbjct: 480 ADPLLRNSQGIRAMATAATREANVLAYNDVFMLIALIAVLTMIWIS 525