BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.1946.p (195 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003777806.1| small-conductance mechanosensitive channel p... 300 8e-80 ref|YP_001899317.1| small-conductance mechanosensitive channel-l... 236 1e-60 ref|YP_004127770.1| mscs mechanosensitive ion channel [Alicyclip... 188 4e-46 ref|YP_987451.1| small-conductance mechanosensitive channel-like... 179 2e-43 ref|YP_002554040.1| small-conductance mechanosensitive channel-l... 178 3e-43 >ref|YP_003777806.1| small-conductance mechanosensitive channel protein [Herbaspirillum seropedicae SmR1] gb|ADJ65898.1| small-conductance mechanosensitive channel protein [Herbaspirillum seropedicae SmR1] Length = 193 Score = 300 bits (768), Expect = 8e-80, Method: Composition-based stats. Identities = 172/195 (88%), Positives = 181/195 (92%), Gaps = 2/195 (1%) Query: 1 MSKFLQSIHDGLPQWLADWFDVLVPTAEVILIVLGALAIMRVARMLIGRLALTYGFPNKA 60 MSKFLQSIHDGLPQWLADWFDVLVPTAEVILIVLGALAIMRVARMLIGRLALTYGFPNKA Sbjct: 1 MSKFLQSIHDGLPQWLADWFDVLVPTAEVILIVLGALAIMRVARMLIGRLALTYGFPNKA 60 Query: 61 AALTQRVLGFFVYGGAMLWALERMGVSGAVLWSAFTGFAAVGAVAFFAVWSVLSNLFCAI 120 AALTQRVLGFFVYGGA+LWALERMGVS A LWSAFTGFAAVGAVAFFA WSVLSNLFCAI Sbjct: 61 AALTQRVLGFFVYGGALLWALERMGVSSAALWSAFTGFAAVGAVAFFAAWSVLSNLFCAI 120 Query: 121 LIFTTSPFRVGDLIEVLEGGDKPGVKGRVLDINLVYTTLLEEGPEQEAKTTLQLPNSMFF 180 LI+TT PFRVGD+IEVLEGGDKPG+KGRVLDINLVYTTL EE + +TTLQLPNS+FF Sbjct: 121 LIYTTQPFRVGDVIEVLEGGDKPGLKGRVLDINLVYTTLAEE--HEGGETTLQLPNSLFF 178 Query: 181 QRIVRRWNEEGGVEF 195 QRIVRRW++ G F Sbjct: 179 QRIVRRWSDGSGSLF 193 >ref|YP_001899317.1| small-conductance mechanosensitive channel-like protein [Ralstonia pickettii 12J] gb|ACD26885.1| small-conductance mechanosensitive channel-like protein [Ralstonia pickettii 12J] Length = 187 Score = 236 bits (602), Expect = 1e-60, Method: Composition-based stats. Identities = 122/187 (65%), Positives = 148/187 (79%), Gaps = 1/187 (0%) Query: 1 MSKFLQSIHDGLPQWLADWFDVLVPTAEVILIVLGALAIMRVARMLIGRLALTYGFPNKA 60 M+K LQ+IH+ LPQWLADWFD+LVP EV+LI GA +R+AR L+ +L TY P K Sbjct: 1 MNKLLQAIHESLPQWLADWFDILVPAIEVVLIAAGAWLAVRIARRLLRKLTNTYSLPGKV 60 Query: 61 AALTQRVLGFFVYGGAMLWALERMGVSGAVLWSAFTGFAAVGAVAFFAVWSVLSNLFCAI 120 AAL+ RV+ VY A LWALERMGVSG VLW+AFTGFA VGAVAFFA WSVLSNLFCA+ Sbjct: 61 AALSLRVVATIVYVAATLWALERMGVSGTVLWTAFTGFATVGAVAFFAAWSVLSNLFCAL 120 Query: 121 LIFTTSPFRVGDLIEVLEGGDKPGVKGRVLDINLVYTTLLEEGPEQEAKTTLQLPNSMFF 180 LI+ T FR+GD++E+LE G+KPG+KGRVLD+NLVYTTLLE E + + LQLPNS+ F Sbjct: 121 LIYVTGAFRIGDVVELLENGEKPGIKGRVLDVNLVYTTLLETTVEGQ-EVVLQLPNSLVF 179 Query: 181 QRIVRRW 187 QR++RRW Sbjct: 180 QRVLRRW 186 >ref|YP_004127770.1| mscs mechanosensitive ion channel [Alicycliphilus denitrificans BC] ref|YP_004387206.1| MscS Mechanosensitive ion channel [Alicycliphilus denitrificans K601] gb|ADV00883.1| MscS Mechanosensitive ion channel [Alicycliphilus denitrificans BC] gb|AEB83690.1| MscS Mechanosensitive ion channel [Alicycliphilus denitrificans K601] Length = 220 Score = 188 bits (477), Expect = 4e-46, Method: Composition-based stats. Identities = 106/187 (56%), Positives = 130/187 (69%), Gaps = 1/187 (0%) Query: 1 MSKFLQSIHDGLPQWLADWFDVLVPTAEVILIVLGALAIMRVARMLIGRLALTYGFPNKA 60 MS L+ + LP W DW DV+VP ++ LIVL AL + + R LI R + Y FP++ Sbjct: 1 MSPMLERLKTILPPWAHDWLDVIVPGMQIALIVLAALLLHYIVRRLIRRASDHYQFPHEL 60 Query: 61 AALTQRVLGFFVYGGAMLWALERMGVSGAVLWSAFTGFAAVGAVAFFAVWSVLSNLFCAI 120 V+ + + GGA L ALER+GVS AVLW+AFTGFA VGAVAFFA WSVLSNLFCA Sbjct: 61 LTPVNAVVRWLILGGAALLALERLGVSAAVLWTAFTGFATVGAVAFFAAWSVLSNLFCAF 120 Query: 121 LIFTTSPFRVGDLIEVLEGGDKPGVKGRVLDINLVYTTLLEEGPEQEAKTTLQLPNSMFF 180 LI T PFRVGD IE+L+ +KPG GRVLDINL+YTT LE+ E T LQ+PN++ F Sbjct: 121 LILTVGPFRVGDHIELLDTAEKPGALGRVLDINLLYTT-LEDATAPEPGTLLQIPNALIF 179 Query: 181 QRIVRRW 187 QR+VRRW Sbjct: 180 QRVVRRW 186 >ref|YP_987451.1| small-conductance mechanosensitive channel-like protein [Acidovorax sp. JS42] gb|ABM43375.1| Small-conductance mechanosensitive channel-like protein [Acidovorax sp. JS42] Length = 222 Score = 179 bits (454), Expect = 2e-43, Method: Composition-based stats. Identities = 100/183 (54%), Positives = 127/183 (69%), Gaps = 1/183 (0%) Query: 5 LQSIHDGLPQWLADWFDVLVPTAEVILIVLGALAIMRVARMLIGRLALTYGFPNKAAALT 64 L + LP W+ DW D++VP +++LIVL AL + R+ +I R + Y FP++ Sbjct: 2 LPRLKTALPVWMHDWLDIIVPGVQIVLIVLAALLLQRLLLRIIMRASDHYQFPHELLTPI 61 Query: 65 QRVLGFFVYGGAMLWALERMGVSGAVLWSAFTGFAAVGAVAFFAVWSVLSNLFCAILIFT 124 V+ + + G A L LER+GVS AVLW+AFTGFA VGAVAFFA WSVLSNLFCA LIFT Sbjct: 62 NAVVRWLILGSAGLLVLERLGVSAAVLWTAFTGFATVGAVAFFAAWSVLSNLFCAFLIFT 121 Query: 125 TSPFRVGDLIEVLEGGDKPGVKGRVLDINLVYTTLLEEGPEQEAKTTLQLPNSMFFQRIV 184 PFRVGD IE+L+ DKPG GRV+DINL+Y T LE+ E T LQ+PN++ FQR+V Sbjct: 122 VGPFRVGDHIELLDTADKPGALGRVMDINLLYIT-LEDATAPEPGTLLQIPNTLIFQRVV 180 Query: 185 RRW 187 RRW Sbjct: 181 RRW 183 >ref|YP_002554040.1| small-conductance mechanosensitive channel-like protein [Acidovorax ebreus TPSY] gb|ACM34040.1| small-conductance mechanosensitive channel-like protein [Acidovorax ebreus TPSY] Length = 222 Score = 178 bits (452), Expect = 3e-43, Method: Composition-based stats. Identities = 100/183 (54%), Positives = 127/183 (69%), Gaps = 1/183 (0%) Query: 5 LQSIHDGLPQWLADWFDVLVPTAEVILIVLGALAIMRVARMLIGRLALTYGFPNKAAALT 64 L + LP W+ DW D++VP +++LIVL AL + R+ +I R + Y FP++ Sbjct: 2 LPRLKTALPVWMHDWLDIIVPGVQIVLIVLAALLLQRLLLRIIMRASDHYQFPHELLIPI 61 Query: 65 QRVLGFFVYGGAMLWALERMGVSGAVLWSAFTGFAAVGAVAFFAVWSVLSNLFCAILIFT 124 V+ + + G A L LER+GVS AVLW+AFTGFA VGAVAFFA WSVLSNLFCA LIFT Sbjct: 62 NAVVRWLILGSAGLLVLERLGVSAAVLWTAFTGFATVGAVAFFAAWSVLSNLFCAFLIFT 121 Query: 125 TSPFRVGDLIEVLEGGDKPGVKGRVLDINLVYTTLLEEGPEQEAKTTLQLPNSMFFQRIV 184 PFRVGD IE+L+ DKPG GRV+DINL+Y T LE+ E T LQ+PN++ FQR+V Sbjct: 122 VGPFRVGDHIELLDTADKPGALGRVMDINLLYIT-LEDATAPEPGTLLQIPNTLIFQRVV 180 Query: 185 RRW 187 RRW Sbjct: 181 RRW 183