BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.1943.p (416 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003777809.1| cobalt-zinc-cadmium resistance cation efflux... 506 e-141 ref|YP_001099898.1| putative RND divalent metal cation efflux me... 416 e-114 ref|YP_546673.1| secretion protein HlyD [Methylobacillus flagell... 405 e-111 ref|YP_004124894.1| efflux transporter, rnd family, mfp subunit ... 399 e-109 ref|YP_001353483.1| CzcB family cobalt/zinc/cadmium efflux trans... 397 e-108 >ref|YP_003777809.1| cobalt-zinc-cadmium resistance cation efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] gb|ADJ65901.1| cobalt-zinc-cadmium resistance cation efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 396 Score = 506 bits (1303), Expect = e-141, Method: Composition-based stats. Identities = 303/383 (79%), Positives = 332/383 (86%), Gaps = 13/383 (3%) Query: 46 HREEGGHKDKTAHDDDKDDEHK------EGEH------KEEGKIALSKEQMAAAGIRVDT 93 H+EEGGHKD H ++K EHK EGEH EEGKIAL+ EQ+A AGI V T Sbjct: 15 HQEEGGHKDNEHHGEEKGSEHKDAAEHGEGEHHDAEPSSEEGKIALNPEQIATAGIEVKT 74 Query: 94 AGPLQIRSTVQLPGEIRFNEDRTAHVVPQVAGIVESVSANLGQKVKKGQVLAVISSSAVS 153 AGP+ + S+VQLPGEIRFNEDRTAHVVPQVAGIVESVSA LGQKV+KGQVLAVI+SSAVS Sbjct: 75 AGPMILNSSVQLPGEIRFNEDRTAHVVPQVAGIVESVSAKLGQKVRKGQVLAVINSSAVS 134 Query: 154 EQRSDLLNAEQRLSLARSAYQREKHLWEQKISAEQDYLQAQAALREAEVAVRNAQQKLRA 213 EQRS++LNAEQRL+LAR+AYQREK LWEQKISAEQDYLQAQAALREAEVAVRNAQQKLRA Sbjct: 135 EQRSEMLNAEQRLALARTAYQREKQLWEQKISAEQDYLQAQAALREAEVAVRNAQQKLRA 194 Query: 214 IGATGTSSGNLNRYEIRAPFDGIVVEKHLGLGEAVREDANAFLISDLSSVWAEIIVSPKD 273 IGAT SSG LNRYEIRAPFDG+VVEKHLGLGEAV+EDANAFLISDLSSVWAEIIVSPKD Sbjct: 195 IGATA-SSGGLNRYEIRAPFDGVVVEKHLGLGEAVKEDANAFLISDLSSVWAEIIVSPKD 253 Query: 274 IQVVRVGEKVVVRATAFDASAEGKISYVGSLIGEQSRTAKAHVVLPNTGDLWRPGLFVNV 333 +Q VR GEKV VRATAFDASA GKISYVGSLIGEQSRTAKAHV+LPN D WRPGLFVNV Sbjct: 254 MQFVRTGEKVTVRATAFDASASGKISYVGSLIGEQSRTAKAHVILPNPDDSWRPGLFVNV 313 Query: 334 EVIAGETQVPVAVAADAIQTVEGENVVFVRDADGFVAKAVSLGRSDGKSVEITSGLDAGT 393 EV++ E QVPVAV+A+A+QTVE ++V+FVR GF A+ V GRS+GK VEI SGL+AG Sbjct: 314 EVVSDEAQVPVAVSAEALQTVEEKSVIFVRVPGGFAAQQVVTGRSNGKFVEIRSGLNAGD 373 Query: 394 GYAATGSFAIKAELGKGSAEHSH 416 YAATGSFAIKAELGKGSAEHSH Sbjct: 374 EYAATGSFAIKAELGKGSAEHSH 396 >ref|YP_001099898.1| putative RND divalent metal cation efflux membrane fusion protein (czcBlike) [Herminiimonas arsenicoxydans] emb|CAL61773.1| putative Secretion protein HlyD (partial Cobalt-zinc-cadmium resistance protein CzcB) [Herminiimonas arsenicoxydans] Length = 404 Score = 416 bits (1069), Expect = e-114, Method: Composition-based stats. Identities = 244/419 (58%), Positives = 316/419 (75%), Gaps = 23/419 (5%) Query: 1 MTKKQTFTIAAIIAIAALVSGIILMKDNSAQGSNARSDAHQEDGEHREEGGHKDKTAHDD 60 + KKQ + AII +++G+IL G+H G D+ HD Sbjct: 6 LNKKQGIAVMAIITAGLVLAGLILFS-----------------GQHAAVG---DEHGHDS 45 Query: 61 D-KDDEHKEGEHKEE--GKIALSKEQMAAAGIRVDTAGPLQIRSTVQLPGEIRFNEDRTA 117 K + H+ GE KEE KI LS Q+ AAG+ + G IR+T+ LPGEI+FNEDRTA Sbjct: 46 RAKGERHEHGESKEERRDKIVLSDAQIKAAGVTMQITGAANIRTTLILPGEIQFNEDRTA 105 Query: 118 HVVPQVAGIVESVSANLGQKVKKGQVLAVISSSAVSEQRSDLLNAEQRLSLARSAYQREK 177 HVVP+++G+V+SV+A+LGQ+VKKGQVLAVI+S+ +SEQRS+LL+A++R++LAR ++REK Sbjct: 106 HVVPRLSGVVQSVAADLGQQVKKGQVLAVIASTGLSEQRSELLSAQKRMALARLTFEREK 165 Query: 178 HLWEQKISAEQDYLQAQAALREAEVAVRNAQQKLRAIGATGTSSGNLNRYEIRAPFDGIV 237 LWE+KISAEQDYLQAQ LRE+E+AV+NA QKL A+GA SSG NRYEIRAPFDG+V Sbjct: 166 KLWEEKISAEQDYLQAQQTLRESEIAVQNASQKLAALGAGSGSSGAFNRYEIRAPFDGMV 225 Query: 238 VEKHLGLGEAVREDANAFLISDLSSVWAEIIVSPKDIQVVRVGEKVVVRATAFDASAEGK 297 V KH+ LGEAV+EDA+ F ISDLSSVWAEI VS KD+ VRVGEKV+V+A+AFD+SA G Sbjct: 226 VAKHIALGEAVKEDADIFTISDLSSVWAEIAVSAKDLNAVRVGEKVIVKASAFDSSASGT 285 Query: 298 ISYVGSLIGEQSRTAKAHVVLPNTGDLWRPGLFVNVEVIAGETQVPVAVAADAIQTVEGE 357 I+YVGSL+GEQ+RTA A V L N WRPGLFV++E++AGET VPV+V+A+A+ ++ + Sbjct: 286 IAYVGSLLGEQTRTATARVTLANPQGTWRPGLFVSIEIVAGETSVPVSVSAEALHSINDK 345 Query: 358 NVVFVRDADGFVAKAVSLGRSDGKSVEITSGLDAGTGYAATGSFAIKAELGKGSAEHSH 416 VVFV+ ADGF+A+ V+ GRSDGKSVEI G+ AG+ YA+TGSF IK+ELGK SAEH+H Sbjct: 346 PVVFVKVADGFIAQQVTTGRSDGKSVEIVKGMQAGSEYASTGSFVIKSELGKASAEHAH 404 >ref|YP_546673.1| secretion protein HlyD [Methylobacillus flagellatus KT] gb|ABE50832.1| secretion protein HlyD [Methylobacillus flagellatus KT] Length = 401 Score = 405 bits (1040), Expect = e-111, Method: Composition-based stats. Identities = 228/416 (54%), Positives = 299/416 (71%), Gaps = 15/416 (3%) Query: 1 MTKKQTFTIAAIIAIAALVSGIILMKDNSAQGSNARSDAHQEDGEHREEGGHKDKTAHDD 60 M KQ + AII + AL++ ++ + A+ + DG H+ + A+ Sbjct: 1 MNHKQNIILFAIIIVTALLAWRMVADTDRAR---------ENDGAE-----HRVQAANSH 46 Query: 61 DKDDEHKEGEHKEEGKIALSKEQMAAAGIRVDTAGPLQIRSTVQLPGEIRFNEDRTAHVV 120 + E + GEH++ + LS Q++AAG+ + AGP +I +T+ LPGEIRFNEDRTAH+V Sbjct: 47 GRQAEERGGEHEDGAIVQLSSAQISAAGLVIAQAGPARILTTLTLPGEIRFNEDRTAHIV 106 Query: 121 PQVAGIVESVSANLGQKVKKGQVLAVISSSAVSEQRSDLLNAEQRLSLARSAYQREKHLW 180 P+V GIVESV+ +LGQ+VKKGQVLA ++S +SE RS+ + A++RL+LAR Y+REK LW Sbjct: 107 PRVDGIVESVAVDLGQQVKKGQVLATLASPGISELRSNAMAAQKRLALARKVYEREKELW 166 Query: 181 EQKISAEQDYLQAQAALREAEVAVRNAQQKLRAIGATGTSSGNLNRYEIRAPFDGIVVEK 240 E +ISAEQDYLQA AL+EAE+ V+NA QKLRAIGA G GNLNRYE+RAPFDG++VE+ Sbjct: 167 EDRISAEQDYLQALQALQEAEITVQNASQKLRAIGA-GDGGGNLNRYELRAPFDGMIVER 225 Query: 241 HLGLGEAVREDANAFLISDLSSVWAEIIVSPKDIQVVRVGEKVVVRATAFDASAEGKISY 300 HL LGE+V+ + N ++DLSSVWAEIIVS KD+ V+VG V VRA AF++ A+G ++Y Sbjct: 226 HLALGESVQANTNVMTLADLSSVWAEIIVSAKDLDTVKVGSTVTVRAAAFESQAQGVVAY 285 Query: 301 VGSLIGEQSRTAKAHVVLPNTGDLWRPGLFVNVEVIAGETQVPVAVAADAIQTVEGENVV 360 VGSL+GEQ+RTA A V LPN WRPGLFVNV ++AGE +VP+AV A A+QT+EG VV Sbjct: 286 VGSLLGEQTRTATARVTLPNPARAWRPGLFVNVAIVAGEAEVPLAVGAQAVQTLEGSQVV 345 Query: 361 FVRDADGFVAKAVSLGRSDGKSVEITSGLDAGTGYAATGSFAIKAELGKGSAEHSH 416 FVR DGF A V GR+DGK VEI SGLD GT Y GSF +K+ELGK A+H+H Sbjct: 346 FVRHGDGFRATPVETGRTDGKLVEIVSGLDVGTAYVLDGSFVLKSELGKAMADHAH 401 >ref|YP_004124894.1| efflux transporter, rnd family, mfp subunit [Alicycliphilus denitrificans BC] gb|ADU98006.1| efflux transporter, RND family, MFP subunit [Alicycliphilus denitrificans BC] Length = 530 Score = 399 bits (1026), Expect = e-109, Method: Composition-based stats. Identities = 232/345 (67%), Positives = 279/345 (80%) Query: 72 KEEGKIALSKEQMAAAGIRVDTAGPLQIRSTVQLPGEIRFNEDRTAHVVPQVAGIVESVS 131 +EEGK+ LS Q+ AA I +DTAG I+S +QLPGEIR NEDRT+HVVP++AG+VESV Sbjct: 186 QEEGKVELSDAQIKAASIGIDTAGAASIKSALQLPGEIRLNEDRTSHVVPRIAGVVESVQ 245 Query: 132 ANLGQKVKKGQVLAVISSSAVSEQRSDLLNAEQRLSLARSAYQREKHLWEQKISAEQDYL 191 A+LGQ VKKGQVLAVI+S A SEQRS+L A++RL+LA++ ++REK LWEQKISAEQDYL Sbjct: 246 ASLGQTVKKGQVLAVIASPAASEQRSELQTAQKRLALAQTTFEREKRLWEQKISAEQDYL 305 Query: 192 QAQAALREAEVAVRNAQQKLRAIGATGTSSGNLNRYEIRAPFDGIVVEKHLGLGEAVRED 251 QA AL EA+VAV NAQQKL A+G SSG LNR+E+RAPFDG+V+EKHL LGEAV+ED Sbjct: 306 QAGQALNEAQVAVANAQQKLSAVGLAPGSSGGLNRFELRAPFDGVVIEKHLSLGEAVKED 365 Query: 252 ANAFLISDLSSVWAEIIVSPKDIQVVRVGEKVVVRATAFDASAEGKISYVGSLIGEQSRT 311 A F +SDL VWAEI V KD+ +VRVGEKV ++ATAFDASA+G I++VG+LIGEQ+R Sbjct: 366 AAVFTVSDLGQVWAEINVPAKDLPLVRVGEKVSIKATAFDASAQGTITFVGALIGEQTRM 425 Query: 312 AKAHVVLPNTGDLWRPGLFVNVEVIAGETQVPVAVAADAIQTVEGENVVFVRDADGFVAK 371 AKA VVL N WRPGLFVNVEVIA ET VPV+VA+DAIQ+V + VVFV+ DGFVA+ Sbjct: 426 AKARVVLANPQGAWRPGLFVNVEVIASETAVPVSVASDAIQSVGDKPVVFVKVKDGFVAQ 485 Query: 372 AVSLGRSDGKSVEITSGLDAGTGYAATGSFAIKAELGKGSAEHSH 416 V LGRSDGK VE+ GL AG YAA GSF +K+ELGK SAEH+H Sbjct: 486 PVQLGRSDGKRVEVLQGLQAGAAYAAAGSFVVKSELGKASAEHTH 530 >ref|YP_001353483.1| CzcB family cobalt/zinc/cadmium efflux transporter membrane fusion protein [Janthinobacterium sp. Marseille] gb|ABR90340.1| cobalt/zinc/cadmium efflux RND transporter, membrane fusion protein, CzcB family [Janthinobacterium sp. Marseille] Length = 422 Score = 397 bits (1020), Expect = e-108, Method: Composition-based stats. Identities = 238/399 (59%), Positives = 300/399 (75%), Gaps = 11/399 (2%) Query: 28 NSAQGSNARSDAHQEDGEHREEGGHKDKTAHDDDK--DDEHKEGEHKE--------EGKI 77 +SA S+ +D+H DGEH E K DD K D EH E K+ E KI Sbjct: 25 SSAGESHGHADSHG-DGEHHAENKSAAKDHSDDHKHADGEHHAEEGKKADAGKEAAENKI 83 Query: 78 ALSKEQMAAAGIRVDTAGPLQIRSTVQLPGEIRFNEDRTAHVVPQVAGIVESVSANLGQK 137 LS Q+ AAG+ + A I++ + LPGEI+FNEDRTAHVVP++AG+V+SV ANLGQ+ Sbjct: 84 LLSDAQIKAAGVSLQVADAAHIKTALVLPGEIQFNEDRTAHVVPRLAGVVQSVPANLGQQ 143 Query: 138 VKKGQVLAVISSSAVSEQRSDLLNAEQRLSLARSAYQREKHLWEQKISAEQDYLQAQAAL 197 VKKGQVLAVI+S+ +SEQRS+L A++RL+LA+ Y+REK LW++KISAEQDYLQAQ L Sbjct: 144 VKKGQVLAVIASTGLSEQRSELFAAQKRLTLAKLTYEREKKLWQEKISAEQDYLQAQQVL 203 Query: 198 REAEVAVRNAQQKLRAIGATGTSSGNLNRYEIRAPFDGIVVEKHLGLGEAVREDANAFLI 257 E E+AV+NA+QKL A+GA ++G NRYEIRAPFDGIVVEKH+ LGEAV+EDAN F + Sbjct: 204 HETEIAVQNARQKLSALGADAGAAGAFNRYEIRAPFDGIVVEKHISLGEAVKEDANIFTL 263 Query: 258 SDLSSVWAEIIVSPKDIQVVRVGEKVVVRATAFDASAEGKISYVGSLIGEQSRTAKAHVV 317 SDLS+VWA+I V KD+ VRVGEKV V+A+AFD++A G ++YVGSL+G Q+RTA A V Sbjct: 264 SDLSTVWADIAVPAKDLNAVRVGEKVTVKASAFDSTATGTVAYVGSLLGVQTRTANARVT 323 Query: 318 LPNTGDLWRPGLFVNVEVIAGETQVPVAVAADAIQTVEGENVVFVRDADGFVAKAVSLGR 377 L N WRPGLFVNVE+++ E +VPVAVAADAIQT+ + V+FV+ ADGF+A+ VS GR Sbjct: 324 LANPQGTWRPGLFVNVEIVSNEVEVPVAVAADAIQTINDKPVIFVKVADGFIAQEVSTGR 383 Query: 378 SDGKSVEITSGLDAGTGYAATGSFAIKAELGKGSAEHSH 416 +DGK VEI GL AG+ YAA GSF IK+ELGK SAEH+H Sbjct: 384 TDGKRVEIVKGLKAGSEYAAGGSFVIKSELGKASAEHTH 422