BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.1936.p (323 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003777825.1| metal-dependent hydrolase [Herbaspirillum se... 532 e-149 ref|YP_003451010.1| amidohydrolase [Azospirillum sp. B510] >gi|2... 449 e-124 ref|YP_001205853.1| putative amidohydrolase [Bradyrhizobium sp. ... 444 e-123 ref|NP_768921.1| 2-pyrone-4,6-dicarbaxylate hydrolase [Bradyrhiz... 359 3e-97 ref|YP_001772348.1| amidohydrolase 2 [Methylobacterium sp. 4-46]... 314 1e-83 >ref|YP_003777825.1| metal-dependent hydrolase [Herbaspirillum seropedicae SmR1] gb|ADJ65917.1| metal-dependent hydrolase of the TIM-barrel fold protein [Herbaspirillum seropedicae SmR1] Length = 309 Score = 532 bits (1371), Expect = e-149, Method: Composition-based stats. Identities = 271/308 (87%), Positives = 289/308 (93%) Query: 15 MVNRRQFLAGSLLACTAARSAIAQSIPNSAGDQPPRLKAPALACDSHHHIYDARFPVSPH 74 M++RR F+AG++LAC RSA AQS+PNSAG QPPRLKAP LACDSHHHIYDARFPVSPH Sbjct: 1 MLDRRSFIAGAMLACAGVRSATAQSVPNSAGSQPPRLKAPMLACDSHHHIYDARFPVSPH 60 Query: 75 WRGGRPDGATVADYRRLMAKLGIARNVVVQPSTYGSDNRCLLDALTQFGAQARGIVVIDD 134 WRGGRPDGATVADYR LMAKLGIAR+VVVQPSTYGSDNRCLLDALTQFGAQARGIVVIDD Sbjct: 61 WRGGRPDGATVADYRLLMAKLGIARHVVVQPSTYGSDNRCLLDALTQFGAQARGIVVIDD 120 Query: 135 DVELAQLKEMDQLGVRGVRVNFLSPQSWGITTVDRLLRTAKRVAPLGWHVQVLMSGAQIA 194 DV+L QLKEMD+LGVRGVRVNFLS QSWG+TTV+RL RTA+R+APLGWHVQVLMSGAQIA Sbjct: 121 DVDLPQLKEMDRLGVRGVRVNFLSAQSWGVTTVERLRRTAERIAPLGWHVQVLMSGAQIA 180 Query: 195 EHEAVLAGLPTPVVIDHLGRIPQPQGLDHPGAQAILRLLAKGNTWIKLSEAYADTLQGPP 254 +HEAVLA LPT VVIDHLGRIPQP GLDHPGAQA+LRLLAKGNTWIKLSE YADTLQGPP Sbjct: 181 QHEAVLAALPTQVVIDHLGRIPQPDGLDHPGAQAVLRLLAKGNTWIKLSEPYADTLQGPP 240 Query: 255 DYPDTSLLARAYVQAAPQRALWGSDWPHPTEKIKPDDAVLFDLLAAWAPDAATREQILVH 314 DYPDTSLLA+AY+QAAPQR LWGSDWPHPTEKIKPDDAVLFDLLAAWAPD A R+QILV Sbjct: 241 DYPDTSLLAKAYLQAAPQRTLWGSDWPHPTEKIKPDDAVLFDLLAAWAPDPALRQQILVS 300 Query: 315 NPAALFGF 322 NPAALFGF Sbjct: 301 NPAALFGF 308 >ref|YP_003451010.1| amidohydrolase [Azospirillum sp. B510] dbj|BAI74466.1| amidohydrolase [Azospirillum sp. B510] Length = 314 Score = 449 bits (1156), Expect = e-124, Method: Composition-based stats. Identities = 229/314 (72%), Positives = 256/314 (81%), Gaps = 5/314 (1%) Query: 15 MVNRRQFLAGSLL--ACTAA---RSAIAQSIPNSAGDQPPRLKAPALACDSHHHIYDARF 69 M+ RR + G + AC A SA+AQ++PNSAG +PP LKAPA ACDSHHHIYDARF Sbjct: 1 MITRRTLIVGGVAGGACAFAATRSSALAQTVPNSAGTEPPALKAPANACDSHHHIYDARF 60 Query: 70 PVSPHWRGGRPDGATVADYRRLMAKLGIARNVVVQPSTYGSDNRCLLDALTQFGAQARGI 129 PVSPHWRGGRP GATVADYR L KL I R+V+VQPSTYG DNRCLLDAL QFG++ARGI Sbjct: 61 PVSPHWRGGRPAGATVADYRLLQKKLSITRHVIVQPSTYGVDNRCLLDALEQFGSEARGI 120 Query: 130 VVIDDDVELAQLKEMDQLGVRGVRVNFLSPQSWGITTVDRLLRTAKRVAPLGWHVQVLMS 189 VVID++ A+L+ M+ LGVRGVRVNFL+PQSWG+TT +RL+ TA R+APLGWH Q+LMS Sbjct: 121 VVIDENTTDAELRRMNDLGVRGVRVNFLTPQSWGVTTPERLVETAARIAPLGWHAQLLMS 180 Query: 190 GAQIAEHEAVLAGLPTPVVIDHLGRIPQPQGLDHPGAQAILRLLAKGNTWIKLSEAYADT 249 G QIA E VL GLP PVV DHLGRIPQP GL HPGAQAILR++ KG WIKLSE YADT Sbjct: 181 GDQIARFEDVLTGLPVPVVFDHLGRIPQPDGLSHPGAQAILRIVGKGRGWIKLSEPYADT 240 Query: 250 LQGPPDYPDTSLLARAYVQAAPQRALWGSDWPHPTEKIKPDDAVLFDLLAAWAPDAATRE 309 GPP Y DTS LARAYVQAAP+R +WGSDWPHPTEK KPDDAVLFDLL WAPDAATRE Sbjct: 241 KLGPPAYADTSALARAYVQAAPERVIWGSDWPHPTEKEKPDDAVLFDLLTGWAPDAATRE 300 Query: 310 QILVHNPAALFGFT 323 +ILV NPA LFGFT Sbjct: 301 RILVANPATLFGFT 314 >ref|YP_001205853.1| putative amidohydrolase [Bradyrhizobium sp. ORS 278] emb|CAL77628.1| conserved hypothetical protein; putative amidohydrolase; putative exported protein [Bradyrhizobium sp. ORS 278] Length = 309 Score = 444 bits (1142), Expect = e-123, Method: Composition-based stats. Identities = 226/308 (73%), Positives = 255/308 (82%) Query: 15 MVNRRQFLAGSLLACTAARSAIAQSIPNSAGDQPPRLKAPALACDSHHHIYDARFPVSPH 74 M+ RR LA A R A A S+PNSAG +PP+LKAPA ACDSHHHIYD+RFPVSPH Sbjct: 1 MITRRNLLAAGAAALIGTRPAAADSVPNSAGSEPPKLKAPANACDSHHHIYDSRFPVSPH 60 Query: 75 WRGGRPDGATVADYRRLMAKLGIARNVVVQPSTYGSDNRCLLDALTQFGAQARGIVVIDD 134 WRGGRPDGATVA+YR L +LG+ R+V+VQPSTYG DNRCLLDAL QFG +ARGIVVID+ Sbjct: 61 WRGGRPDGATVAEYRLLQKRLGVVRHVIVQPSTYGVDNRCLLDALDQFGREARGIVVIDE 120 Query: 135 DVELAQLKEMDQLGVRGVRVNFLSPQSWGITTVDRLLRTAKRVAPLGWHVQVLMSGAQIA 194 ++ A+LK MDQ GVRGVRVNFL+PQSWG+TT +RL +TAKR+APLGWHVQVLMSG QIA Sbjct: 121 SIDDAELKRMDQRGVRGVRVNFLTPQSWGVTTAERLEQTAKRIAPLGWHVQVLMSGDQIA 180 Query: 195 EHEAVLAGLPTPVVIDHLGRIPQPQGLDHPGAQAILRLLAKGNTWIKLSEAYADTLQGPP 254 +HEAVLA LP PVV DHLGRIPQP GL HPGAQA+LRL KG WIKLSE YADT GPP Sbjct: 181 QHEAVLAALPVPVVFDHLGRIPQPIGLAHPGAQAMLRLADKGRGWIKLSEPYADTRLGPP 240 Query: 255 DYPDTSLLARAYVQAAPQRALWGSDWPHPTEKIKPDDAVLFDLLAAWAPDAATREQILVH 314 DY DTS +ARA VQA P+R +WGSDWPHPTEK KPDDA+LFDLL+ WAPD A R++ILV Sbjct: 241 DYADTSAVARACVQAVPERVIWGSDWPHPTEKEKPDDALLFDLLSDWAPDDAIRDKILVG 300 Query: 315 NPAALFGF 322 NPA LFGF Sbjct: 301 NPAKLFGF 308 >ref|NP_768921.1| 2-pyrone-4,6-dicarbaxylate hydrolase [Bradyrhizobium japonicum USDA 110] dbj|BAC47546.1| blr2281 [Bradyrhizobium japonicum USDA 110] Length = 350 Score = 359 bits (921), Expect = 3e-97, Method: Composition-based stats. Identities = 187/315 (59%), Positives = 227/315 (72%), Gaps = 10/315 (3%) Query: 16 VNRRQFLAGSLLACTAARSA-------IAQSIPNSAGDQPPRLKAPALACDSHHHIYDAR 68 + RR + G +LAC+ A + Q++PNS G PRL A A ACD HHHIYD R Sbjct: 38 ITRRGVVMG-MLACSTGALAAGAMAAGVEQAVPNSTGSAAPRLDARAGACDCHHHIYDER 96 Query: 69 FPVSPHWRGGRPDGATVADYRRLMAKLGIARNVVVQPSTYGSDNRCLLDALTQFGAQARG 128 FPVSPHWR G P GATVADYR L +LG R+VVVQPSTYG DNRCL+DAL Q GA +RG Sbjct: 97 FPVSPHWRQGFPPGATVADYRLLQQRLGTTRSVVVQPSTYGIDNRCLVDALGQLGASSRG 156 Query: 129 IVVIDDDVELAQLKEMDQLGVRGVRVNFLSPQSWGITTVDRLLRTAKRVAPLGWHVQVLM 188 + V+D DV+ A+L+ + GVR +RVNF+SPQ+WG TT L A RV+PLGWHVQ+LM Sbjct: 157 VAVVDTDVKDAELRALADAGVRAIRVNFVSPQTWGTTTPQMLTTLAARVSPLGWHVQILM 216 Query: 189 SGAQIAEHEAVLAGLPTPVVIDHLGRIPQPQGLDHPGAQAILRLLAKGNTWIKLSEAYAD 248 +G Q+A HE+V+ LPT VVIDHLGRIPQP+G+ HP AI R+L G TW+K++E Y D Sbjct: 217 TGDQVAAHESVIRSLPTRVVIDHLGRIPQPEGVKHPAFAAIRRMLDGGRTWVKVTEPYED 276 Query: 249 TLQGPPDYPDTSLLARAYVQAAPQRALWGSDWPHPTEK-IKPDDAVLFDLLAAWAPDAAT 307 + GPP Y D+ +ARAYV AAP+R LWG+DWPHPT++ KPDDA L DLLA W PD T Sbjct: 277 SKLGPP-YADSGDVARAYVHAAPERILWGTDWPHPTQRGTKPDDAQLLDLLADWVPDEPT 335 Query: 308 REQILVHNPAALFGF 322 R +ILV NPA LFGF Sbjct: 336 RRRILVENPAELFGF 350 >ref|YP_001772348.1| amidohydrolase 2 [Methylobacterium sp. 4-46] gb|ACA19914.1| amidohydrolase 2 [Methylobacterium sp. 4-46] Length = 321 Score = 314 bits (805), Expect = 1e-83, Method: Composition-based stats. Identities = 155/284 (54%), Positives = 197/284 (69%) Query: 39 SIPNSAGDQPPRLKAPALACDSHHHIYDARFPVSPHWRGGRPDGATVADYRRLMAKLGIA 98 ++P SAG + P+ + PA ACDSH HI+ RFP SPHW+G + VA YR ++G + Sbjct: 35 AVPFSAGTEQPKTEVPAQACDSHIHIFSTRFPASPHWKGQPVSDSDVAAYRLFQKRIGTS 94 Query: 99 RNVVVQPSTYGSDNRCLLDALTQFGAQARGIVVIDDDVELAQLKEMDQLGVRGVRVNFLS 158 R VVV PSTYG DNR LD + Q GA AR +VV+D DV A+L++M G G+RVNF++ Sbjct: 95 RTVVVTPSTYGIDNRATLDGVAQLGASARAVVVVDLDVTEAELRDMAARGTVGIRVNFVT 154 Query: 159 PQSWGITTVDRLLRTAKRVAPLGWHVQVLMSGAQIAEHEAVLAGLPTPVVIDHLGRIPQP 218 PQSWG+TT +RL A++V P GWHVQV M+G QIA+H VLA LPTP+VIDHL R+ Sbjct: 155 PQSWGVTTAERLEAMARKVQPFGWHVQVYMTGDQIADHAEVLARLPTPLVIDHLARLLPG 214 Query: 219 QGLDHPGAQAILRLLAKGNTWIKLSEAYADTLQGPPDYPDTSLLARAYVQAAPQRALWGS 278 QGL+H + LL G TW+KLS AY +T GPP Y D + +A+ + +AAP+R +WGS Sbjct: 215 QGLNHRAVPVVRNLLDGGRTWMKLSGAYLNTTSGPPTYADATDVAKLFAKAAPERMVWGS 274 Query: 279 DWPHPTEKIKPDDAVLFDLLAAWAPDAATREQILVHNPAALFGF 322 DWPH EK PDDA LFDLLA WAP A R +IL+ NPAAL+GF Sbjct: 275 DWPHRGEKHMPDDAGLFDLLAEWAPSEAARRRILIDNPAALYGF 318