BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.1931.p (273 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003777827.1| D-3-phosphoglycerate dehydrogenase [Herbaspi... 403 e-110 ref|YP_004230890.1| D-isomer specific 2-hydroxyacid dehydrogenas... 329 2e-88 ref|ZP_03585348.1| D-isomer specific 2-hydroxyacid dehydrogenase... 325 6e-87 ref|YP_001584674.1| D-isomer specific 2-hydroxyacid dehydrogenas... 322 4e-86 ref|ZP_03573558.1| D-isomer specific 2-hydroxyacid dehydrogenase... 313 2e-83 >ref|YP_003777827.1| D-3-phosphoglycerate dehydrogenase [Herbaspirillum seropedicae SmR1] gb|ADJ65919.1| D-3-phosphoglycerate dehydrogenase protein [Herbaspirillum seropedicae SmR1] Length = 308 Score = 403 bits (1036), Expect = e-110, Method: Composition-based stats. Identities = 240/266 (90%), Positives = 252/266 (94%) Query: 1 MVRLVQQHLPVAIIVRYGGVSARVMDAAPGLRVISKHGTGIDSIDTEAAKARGIVVKAAA 60 MV+L Q H PVAIIVRYGGVSARVMDAAP LRVISKHGTGIDSID++AA+ RGI VKAAA Sbjct: 41 MVKLAQLHQPVAIIVRYGGVSARVMDAAPILRVISKHGTGIDSIDSQAAQQRGIAVKAAA 100 Query: 61 GANAPAVAEHTWALIMACAKNVVGLDQRMREGHWDKSTHKSLELRGRTLGLVGIGAIARR 120 GANAPAVAEHTWALIMACAKNVVGLDQRMREGHWDKSTHKSLEL+GRTLGLVG+GAI RR Sbjct: 101 GANAPAVAEHTWALIMACAKNVVGLDQRMREGHWDKSTHKSLELQGRTLGLVGLGAIGRR 160 Query: 121 VAAVAVALEMPVLAYDPYAKDAPQGVRLVDLDTLFAQSDVVSLHCPLTADNKHMINAQSL 180 VAAVA AL MPVLA+DPYAK+APQGV+LVDLDTLFAQSDVVSLHCPLTA+NKHMINAQSL Sbjct: 161 VAAVAAALGMPVLAHDPYAKEAPQGVQLVDLDTLFAQSDVVSLHCPLTAENKHMINAQSL 220 Query: 181 ARMKDGTILVNTARGGLIDEAALIAALASGKLRAAGLDSFEKEPFTAPHPLQDVSNAILS 240 ARMKDG ILVNTARGGLIDE ALIAAL SGKLRAAGLDSFEKEPFTAPHPLQ V NA+LS Sbjct: 221 ARMKDGAILVNTARGGLIDEQALIAALDSGKLRAAGLDSFEKEPFTAPHPLQRVGNAVLS 280 Query: 241 PHIGGVTADAYIAMGTGAASNVLAVL 266 PHIGGVT+DAYIAMGTGAASNVLAVL Sbjct: 281 PHIGGVTSDAYIAMGTGAASNVLAVL 306 >ref|YP_004230890.1| D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding protein [Burkholderia sp. CCGE1001] gb|ADX57830.1| D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding protein protein [Burkholderia sp. CCGE1001] Length = 315 Score = 329 bits (844), Expect = 2e-88, Method: Composition-based stats. Identities = 189/269 (70%), Positives = 221/269 (82%) Query: 1 MVRLVQQHLPVAIIVRYGGVSARVMDAAPGLRVISKHGTGIDSIDTEAAKARGIVVKAAA 60 +V L QH PVAIIVRYG ++AR+MDA L+VISKHG+GID ID AA RGI V+AA Sbjct: 42 LVALSAQHNPVAIIVRYGKINARIMDAGHKLQVISKHGSGIDVIDQAAAAERGIAVRAAV 101 Query: 61 GANAPAVAEHTWALIMACAKNVVGLDQRMREGHWDKSTHKSLELRGRTLGLVGIGAIARR 120 GANA AV+EH WALI+ACAK+V LD RMR+GHWDKSTHKS+EL GRTLGLVG+GAI RR Sbjct: 102 GANAAAVSEHAWALILACAKSVPQLDLRMRDGHWDKSTHKSVELDGRTLGLVGLGAIGRR 161 Query: 121 VAAVAVALEMPVLAYDPYAKDAPQGVRLVDLDTLFAQSDVVSLHCPLTADNKHMINAQSL 180 VA+V +A M V+A+DPYAKDAPQG +LV LD L+AQSDVVSLHCPLTA+N+ M+N ++L Sbjct: 162 VASVGIAFGMNVIAFDPYAKDAPQGTQLVSLDELYAQSDVVSLHCPLTAENRQMLNRETL 221 Query: 181 ARMKDGTILVNTARGGLIDEAALIAALASGKLRAAGLDSFEKEPFTAPHPLQDVSNAILS 240 AR+K G ILVNTARGGLIDEAAL ALASG++RAAGLDSF+ EP PHP Q + N ILS Sbjct: 222 ARLKQGAILVNTARGGLIDEAALAEALASGQIRAAGLDSFDVEPMATPHPFQRIPNVILS 281 Query: 241 PHIGGVTADAYIAMGTGAASNVLAVLNEQ 269 PHIGGV+ AY+ MG GAA+NVLAVL EQ Sbjct: 282 PHIGGVSDAAYVNMGRGAAANVLAVLEEQ 310 >ref|ZP_03585348.1| D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding [Burkholderia multivorans CGD1] gb|EED99939.1| D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding [Burkholderia multivorans CGD1] Length = 315 Score = 325 bits (832), Expect = 6e-87, Method: Composition-based stats. Identities = 192/269 (71%), Positives = 223/269 (82%) Query: 1 MVRLVQQHLPVAIIVRYGGVSARVMDAAPGLRVISKHGTGIDSIDTEAAKARGIVVKAAA 60 +V L +H PVAIIVRYG ++ARVMDAA L+VISKHG+GID ID +AA ARGI V+AA Sbjct: 42 IVALCAKHKPVAIIVRYGKINARVMDAAENLQVISKHGSGIDVIDQDAAAARGIAVRAAV 101 Query: 61 GANAPAVAEHTWALIMACAKNVVGLDQRMREGHWDKSTHKSLELRGRTLGLVGIGAIARR 120 GANA AVAEH WALI+ACAK+V LD RMREGHWDK+THKS+EL GRTLGLVG+GAI RR Sbjct: 102 GANAAAVAEHAWALILACAKSVPHLDMRMREGHWDKATHKSVELDGRTLGLVGLGAIGRR 161 Query: 121 VAAVAVALEMPVLAYDPYAKDAPQGVRLVDLDTLFAQSDVVSLHCPLTADNKHMINAQSL 180 VAA+ VA M VLA+DP+AK+AP GVRLV LDTL+A+SDVVS+HCPLTADN+ M+N +L Sbjct: 162 VAAIGVAFGMKVLAFDPFAKEAPAGVRLVPLDTLYAESDVVSMHCPLTADNRRMLNRDTL 221 Query: 181 ARMKDGTILVNTARGGLIDEAALIAALASGKLRAAGLDSFEKEPFTAPHPLQDVSNAILS 240 AR K G ILVNTARGGLIDEAAL AL SG LRAA LDSF+ EP T+PHP Q + N ILS Sbjct: 222 ARFKRGAILVNTARGGLIDEAALAEALTSGPLRAAALDSFDVEPMTSPHPFQQLPNVILS 281 Query: 241 PHIGGVTADAYIAMGTGAASNVLAVLNEQ 269 PHIGGV+ AY+ MG GAA+NVLAVL+E+ Sbjct: 282 PHIGGVSDAAYVNMGKGAAANVLAVLDER 310 >ref|YP_001584674.1| D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding [Burkholderia multivorans ATCC 17616] ref|YP_001948207.1| D-3-phosphoglycerate dehydrogenase [Burkholderia multivorans ATCC 17616] gb|ABX18382.1| D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding [Burkholderia multivorans ATCC 17616] dbj|BAG45671.1| D-3-phosphoglycerate dehydrogenase [Burkholderia multivorans ATCC 17616] Length = 315 Score = 322 bits (825), Expect = 4e-86, Method: Composition-based stats. Identities = 191/269 (71%), Positives = 222/269 (82%) Query: 1 MVRLVQQHLPVAIIVRYGGVSARVMDAAPGLRVISKHGTGIDSIDTEAAKARGIVVKAAA 60 +V L +H PVAIIVRYG ++ARVMDAA L+VISKHG+GID ID +AA ARGI V+AA Sbjct: 42 IVALCVKHKPVAIIVRYGKINARVMDAAENLQVISKHGSGIDVIDQDAAAARGIAVRAAV 101 Query: 61 GANAPAVAEHTWALIMACAKNVVGLDQRMREGHWDKSTHKSLELRGRTLGLVGIGAIARR 120 GANA AVAEH WALI+ACAK+V LD RMREGHWDK+THKS+EL GRTLGLVG+GAI RR Sbjct: 102 GANAAAVAEHAWALILACAKSVPQLDMRMREGHWDKATHKSVELDGRTLGLVGLGAIGRR 161 Query: 121 VAAVAVALEMPVLAYDPYAKDAPQGVRLVDLDTLFAQSDVVSLHCPLTADNKHMINAQSL 180 VAA+ VA M VLA+DP+AK+AP GV LV LDTL+A+SDVVS+HCPLTADN+ M+N +L Sbjct: 162 VAAIGVAFGMKVLAFDPFAKEAPAGVTLVPLDTLYAESDVVSMHCPLTADNRRMLNRDTL 221 Query: 181 ARMKDGTILVNTARGGLIDEAALIAALASGKLRAAGLDSFEKEPFTAPHPLQDVSNAILS 240 AR K G ILVNTARGGLIDEAAL AL SG LRAA LDSF+ EP T+PHP Q + N ILS Sbjct: 222 ARFKRGAILVNTARGGLIDEAALAEALTSGPLRAAALDSFDVEPMTSPHPFQQLPNVILS 281 Query: 241 PHIGGVTADAYIAMGTGAASNVLAVLNEQ 269 PHIGGV+ AY+ MG GAA+NVLAVL+E+ Sbjct: 282 PHIGGVSDAAYVNMGKGAAANVLAVLDER 310 >ref|ZP_03573558.1| D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding [Burkholderia multivorans CGD2M] ref|ZP_03579211.1| D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding [Burkholderia multivorans CGD2] gb|EEE06642.1| D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding [Burkholderia multivorans CGD2] gb|EEE12262.1| D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding [Burkholderia multivorans CGD2M] Length = 315 Score = 313 bits (802), Expect = 2e-83, Method: Composition-based stats. Identities = 192/269 (71%), Positives = 224/269 (83%) Query: 1 MVRLVQQHLPVAIIVRYGGVSARVMDAAPGLRVISKHGTGIDSIDTEAAKARGIVVKAAA 60 +V L QH PVAIIVRYG ++AR+MDAA L+VISKHG+GID ID +AA ARGI V+AA Sbjct: 42 IVALCTQHKPVAIIVRYGKINARIMDAAENLQVISKHGSGIDVIDQDAAAARGIAVRAAV 101 Query: 61 GANAPAVAEHTWALIMACAKNVVGLDQRMREGHWDKSTHKSLELRGRTLGLVGIGAIARR 120 GANA AVAEH WAL++ACAK+V LD RMR GHWDK+THKS+EL GRTLGLVG+GAI RR Sbjct: 102 GANAAAVAEHAWALMLACAKSVPQLDSRMRAGHWDKATHKSVELDGRTLGLVGLGAIGRR 161 Query: 121 VAAVAVALEMPVLAYDPYAKDAPQGVRLVDLDTLFAQSDVVSLHCPLTADNKHMINAQSL 180 VAA+ A M VLA+DP+AK+AP GV+LV LDTL+A+SDVVSLHCPLTADN+ M+N +L Sbjct: 162 VAAIGAAFGMKVLAFDPFAKEAPAGVKLVPLDTLYAESDVVSLHCPLTADNRRMLNRDAL 221 Query: 181 ARMKDGTILVNTARGGLIDEAALIAALASGKLRAAGLDSFEKEPFTAPHPLQDVSNAILS 240 AR K G ILVNTARGGLIDEAAL ALASG+LRAAGLDSF+ EP TAPHP Q + N ILS Sbjct: 222 ARFKRGAILVNTARGGLIDEAALADALASGQLRAAGLDSFDVEPMTAPHPFQQIPNVILS 281 Query: 241 PHIGGVTADAYIAMGTGAASNVLAVLNEQ 269 PH+GGV+ AY+ MG GAA+NVLAVLNE+ Sbjct: 282 PHVGGVSDAAYVNMGKGAAANVLAVLNER 310