BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.1903.p (341 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value emb|CBJ38030.1| putative aliphatic nitrilase (nitA) [Ralstonia s... 576 e-162 ref|NP_519944.1| nitrilase [Ralstonia solanacearum GMI1000] >gi|... 576 e-162 ref|YP_004235808.1| nitrilase [Acidovorax avenae subsp. avenae A... 569 e-160 ref|YP_998929.1| Nitrilase/cyanide hydratase and apolipoprotein ... 558 e-157 ref|ZP_05974243.1| nitrilase family protein [Providencia rustigi... 556 e-156 >emb|CBJ38030.1| putative aliphatic nitrilase (nitA) [Ralstonia solanacearum CMR15] Length = 343 Score = 576 bits (1485), Expect = e-162, Method: Composition-based stats. Identities = 278/321 (86%), Positives = 304/321 (94%) Query: 1 MLELQRFKAAAVQAAPVFLDTDATVDKVVALIAEAAGHGAKLVAFPEVFVAGYPYWSWIM 60 MLEL +FKAAAVQAAPVFLDTDATVDKV LI EAA +GA+LVAFPEVFVAGYPYWSWIM Sbjct: 1 MLELPQFKAAAVQAAPVFLDTDATVDKVCRLIQEAAENGARLVAFPEVFVAGYPYWSWIM 60 Query: 61 NPIEGSSWFERLVRSAIEIPGPEISKIAQAAARHQINVVVGVNERNRHGIGTIYNTVVTI 120 NPIEGS WFE+L RSAIE+PGPEI+KIAQAAARH+INVV+GVNER+R+GIGT+YNT+VTI Sbjct: 61 NPIEGSPWFEKLARSAIELPGPEIAKIAQAAARHRINVVIGVNERSRYGIGTLYNTIVTI 120 Query: 121 SDDGRIIGRHRKLVPTWAEKLTWAPGDASGLRVHQTSIGPLGALACGENTNTLARFSLLA 180 +DDGRI+GRHRKLVPTWAEKLTWAPGDAS LRVH TSIGPLGALACGENTNTLARF+LLA Sbjct: 121 ADDGRILGRHRKLVPTWAEKLTWAPGDASALRVHDTSIGPLGALACGENTNTLARFALLA 180 Query: 181 QGELVHVASYIALPVAPADYDMAEAIKVRAAAHCFEGKVFTIVSCSTVSEEIIQAMEASH 240 QGELVHVASYI+LPVAP DYDMAEAIKVRAAAHCFEGKVFT+VSCSTVSEEII+AM A+H Sbjct: 181 QGELVHVASYISLPVAPPDYDMAEAIKVRAAAHCFEGKVFTVVSCSTVSEEIIEAMAATH 240 Query: 241 PQARALLSRRNSAFSGVLGPDGRVIGSPLIDEEGIVYADIDLSRCIQPRQMHDITGHYNR 300 PQ+R LL+RRNSAFSG+LGPDGRVIG PLID+EGIVYADIDLSRCIQPRQMHDITGHYNR Sbjct: 241 PQSRELLARRNSAFSGILGPDGRVIGQPLIDQEGIVYADIDLSRCIQPRQMHDITGHYNR 300 Query: 301 FDVFNLNVNRQPLQAAHFSDG 321 FDVF+L VNR+PL+AA F D Sbjct: 301 FDVFDLRVNRKPLRAAQFDDA 321 >ref|NP_519944.1| nitrilase [Ralstonia solanacearum GMI1000] emb|CAD15525.1| hypothetical protein RSc1823 [Ralstonia solanacearum GMI1000] Length = 343 Score = 576 bits (1484), Expect = e-162, Method: Composition-based stats. Identities = 277/321 (86%), Positives = 305/321 (95%) Query: 1 MLELQRFKAAAVQAAPVFLDTDATVDKVVALIAEAAGHGAKLVAFPEVFVAGYPYWSWIM 60 MLEL +FKAAAVQAAPVFLDTDATVDKV LI EAA +GA+LVAFPEVFVAGYPYWSWIM Sbjct: 1 MLELPQFKAAAVQAAPVFLDTDATVDKVCRLIQEAAENGARLVAFPEVFVAGYPYWSWIM 60 Query: 61 NPIEGSSWFERLVRSAIEIPGPEISKIAQAAARHQINVVVGVNERNRHGIGTIYNTVVTI 120 NP+EGS WFE+L RSAIE+PGPEI+KIAQAAARH+INVV+GVNER+R+GIGT+YNT+VTI Sbjct: 61 NPVEGSPWFEKLARSAIELPGPEIAKIAQAAARHRINVVIGVNERSRYGIGTLYNTIVTI 120 Query: 121 SDDGRIIGRHRKLVPTWAEKLTWAPGDASGLRVHQTSIGPLGALACGENTNTLARFSLLA 180 +DDGRI+GRHRKLVPTWAEKLTWAPGDAS LRVH TSIGPLGALACGENTNTLARF+LLA Sbjct: 121 ADDGRILGRHRKLVPTWAEKLTWAPGDASALRVHDTSIGPLGALACGENTNTLARFALLA 180 Query: 181 QGELVHVASYIALPVAPADYDMAEAIKVRAAAHCFEGKVFTIVSCSTVSEEIIQAMEASH 240 QGELVHVASYI+LPVAP DYDMAEAIKVRAAAHCFEGKVFT+VSCSTVSEEII+AM A+H Sbjct: 181 QGELVHVASYISLPVAPPDYDMAEAIKVRAAAHCFEGKVFTVVSCSTVSEEIIEAMAATH 240 Query: 241 PQARALLSRRNSAFSGVLGPDGRVIGSPLIDEEGIVYADIDLSRCIQPRQMHDITGHYNR 300 PQ+RALL+RRNSAFSG+LGPDGRVIG PLID+EGIVYADIDLSRCIQPRQMHDITGHYNR Sbjct: 241 PQSRALLARRNSAFSGILGPDGRVIGQPLIDQEGIVYADIDLSRCIQPRQMHDITGHYNR 300 Query: 301 FDVFNLNVNRQPLQAAHFSDG 321 FD+F+L VNR+PL+AA F D Sbjct: 301 FDIFDLRVNRKPLRAAQFDDA 321 >ref|YP_004235808.1| nitrilase [Acidovorax avenae subsp. avenae ATCC 19860] gb|ADX47241.1| Nitrilase [Acidovorax avenae subsp. avenae ATCC 19860] Length = 344 Score = 569 bits (1467), Expect = e-160, Method: Composition-based stats. Identities = 277/326 (84%), Positives = 302/326 (92%) Query: 1 MLELQRFKAAAVQAAPVFLDTDATVDKVVALIAEAAGHGAKLVAFPEVFVAGYPYWSWIM 60 MLEL +FKAAAVQAAPVFLDTDATVDKV LIAEAAG+GA+LVAFPEVFVAGYPYWSWI Sbjct: 1 MLELPKFKAAAVQAAPVFLDTDATVDKVCRLIAEAAGNGAQLVAFPEVFVAGYPYWSWIA 60 Query: 61 NPIEGSSWFERLVRSAIEIPGPEISKIAQAAARHQINVVVGVNERNRHGIGTIYNTVVTI 120 NPI+GS WFERL RSAIEIPGPEI+KIAQAAARH+ +VV+GVNER+RHGIGTIYNT+VTI Sbjct: 61 NPIDGSPWFERLARSAIEIPGPEIAKIAQAAARHKTHVVIGVNERSRHGIGTIYNTLVTI 120 Query: 121 SDDGRIIGRHRKLVPTWAEKLTWAPGDASGLRVHQTSIGPLGALACGENTNTLARFSLLA 180 DDGRI+GRHRKLVPTWAEKLTWAPGDAS LRVH+TSIGPLG+LACGENTNTLARF+LL+ Sbjct: 121 GDDGRILGRHRKLVPTWAEKLTWAPGDASALRVHRTSIGPLGSLACGENTNTLARFALLS 180 Query: 181 QGELVHVASYIALPVAPADYDMAEAIKVRAAAHCFEGKVFTIVSCSTVSEEIIQAMEASH 240 QGELVHVASYI+LPVAP DYDMAEAIKVRAAAHCFEGK+FTIVSCSTVSEEII AME + Sbjct: 181 QGELVHVASYISLPVAPPDYDMAEAIKVRAAAHCFEGKLFTIVSCSTVSEEIITAMEPLN 240 Query: 241 PQARALLSRRNSAFSGVLGPDGRVIGSPLIDEEGIVYADIDLSRCIQPRQMHDITGHYNR 300 P+AR LL+R+NSAFSG LGPDGRVIG PLID+EGI YADIDL+RCIQPRQMHDITGHYNR Sbjct: 241 PKARELLTRKNSAFSGFLGPDGRVIGQPLIDDEGITYADIDLARCIQPRQMHDITGHYNR 300 Query: 301 FDVFNLNVNRQPLQAAHFSDGDAVMD 326 FDVF+L VNR+PLQAA F D A D Sbjct: 301 FDVFDLRVNRRPLQAARFDDEPADND 326 >ref|YP_998929.1| Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase [Verminephrobacter eiseniae EF01-2] gb|ABM59911.1| Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase [Verminephrobacter eiseniae EF01-2] Length = 339 Score = 558 bits (1439), Expect = e-157, Method: Composition-based stats. Identities = 276/335 (82%), Positives = 301/335 (89%), Gaps = 3/335 (0%) Query: 1 MLELQRFKAAAVQAAPVFLDTDATVDKVVALIAEAAGHGAKLVAFPEVFVAGYPYWSWIM 60 MLEL FKAAAVQ APVFLDTDATVDKVV LI EAAG+GA+LVAFPEVFV+ YPYWSWI Sbjct: 1 MLELPHFKAAAVQTAPVFLDTDATVDKVVRLIGEAAGNGARLVAFPEVFVSAYPYWSWIG 60 Query: 61 NPIEGSSWFERLVRSAIEIPGPEISKIAQAAARHQINVVVGVNERNRHGIGTIYNTVVTI 120 NP+EGS+WFE+LVRSAIEIPGPEI+KIAQAAARH+INVVVG NERNRHGIGTIYNT+VTI Sbjct: 61 NPVEGSAWFEKLVRSAIEIPGPEIAKIAQAAARHRINVVVGANERNRHGIGTIYNTLVTI 120 Query: 121 SDDGRIIGRHRKLVPTWAEKLTWAPGDASGLRVHQTSIGPLGALACGENTNTLARFSLLA 180 ++DGRI+GRHRKLVPTWAEKLTWAPGDAS LRVH TSIGPLG+LACGENTNTLARFSL+A Sbjct: 121 AEDGRILGRHRKLVPTWAEKLTWAPGDASALRVHDTSIGPLGSLACGENTNTLARFSLIA 180 Query: 181 QGELVHVASYIALPVAPADYDMAEAIKVRAAAHCFEGKVFTIVSCSTVSEEIIQAMEASH 240 QGELVHVASYI+LPVAP DYDMAEAIKVRAAAH FEGK+FTIVSCST+SEEII A+E Sbjct: 181 QGELVHVASYISLPVAPPDYDMAEAIKVRAAAHSFEGKLFTIVSCSTISEEIIAALEGDF 240 Query: 241 PQARALLSRRNSAFSGVLGPDGRVIGSPLIDEEGIVYADIDLSRCIQPRQMHDITGHYNR 300 P+AR LL R+NSAFS VLGPDGRVIG PLIDEEGIVYADIDLSRCIQPRQMHDI+GHYNR Sbjct: 241 PKARELLLRKNSAFSAVLGPDGRVIGQPLIDEEGIVYADIDLSRCIQPRQMHDISGHYNR 300 Query: 301 FDVFNLNVNRQPLQAAHFSDGDAVMDEVPVEAVAE 335 FDVF+L VNR+ LQA F D + P+E V E Sbjct: 301 FDVFDLRVNRRSLQAVRFDDA---AEPAPIEPVTE 332 >ref|ZP_05974243.1| nitrilase family protein [Providencia rustigianii DSM 4541] gb|EFB70839.1| nitrilase family protein [Providencia rustigianii DSM 4541] Length = 343 Score = 556 bits (1434), Expect = e-156, Method: Composition-based stats. Identities = 264/337 (78%), Positives = 303/337 (89%) Query: 1 MLELQRFKAAAVQAAPVFLDTDATVDKVVALIAEAAGHGAKLVAFPEVFVAGYPYWSWIM 60 ML L +FKAAAVQAAPVFLDT+ATVDKV LI EAA +GAKLVAFPEVFV+GYPYWSW+M Sbjct: 1 MLNLPQFKAAAVQAAPVFLDTNATVDKVCRLIEEAADNGAKLVAFPEVFVSGYPYWSWVM 60 Query: 61 NPIEGSSWFERLVRSAIEIPGPEISKIAQAAARHQINVVVGVNERNRHGIGTIYNTVVTI 120 NPI+GS WFE+L +SAIE+PGPEI +IAQAAARH INVV+GVNERN +GI T+YNT+VTI Sbjct: 61 NPIDGSPWFEKLCKSAIEVPGPEIKRIAQAAARHHINVVIGVNERNPNGIATLYNTLVTI 120 Query: 121 SDDGRIIGRHRKLVPTWAEKLTWAPGDASGLRVHQTSIGPLGALACGENTNTLARFSLLA 180 SD+GRI+GRHRKLVPTWAEKLTWA GDAS L+VHQTSIGPLGALACGENTNTLARFSLLA Sbjct: 121 SDEGRILGRHRKLVPTWAEKLTWANGDASSLKVHQTSIGPLGALACGENTNTLARFSLLA 180 Query: 181 QGELVHVASYIALPVAPADYDMAEAIKVRAAAHCFEGKVFTIVSCSTVSEEIIQAMEASH 240 QGELVH+ASYIALPVAP DYDMAEAI++RA+AHCFEGKVFT+VSCSTVS+EII+AM SH Sbjct: 181 QGELVHIASYIALPVAPKDYDMAEAIRLRASAHCFEGKVFTVVSCSTVSDEIIEAMADSH 240 Query: 241 PQARALLSRRNSAFSGVLGPDGRVIGSPLIDEEGIVYADIDLSRCIQPRQMHDITGHYNR 300 P++R LL+R NSAFSG++GPDGRV+G PLID+EGIVY +IDL+RCIQPRQMHDITGHYNR Sbjct: 241 PESRELLARPNSAFSGIIGPDGRVVGEPLIDKEGIVYGEIDLNRCIQPRQMHDITGHYNR 300 Query: 301 FDVFNLNVNRQPLQAAHFSDGDAVMDEVPVEAVAEIQ 337 FD+F+L VNR+PL AA F D +DE+ A A+ Q Sbjct: 301 FDIFDLQVNRRPLTAARFHDSGQNVDELNQFAEADEQ 337