BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.1852.p (308 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003777873.1| metal-dependent hydrolase [Herbaspirillum se... 485 e-135 ref|YP_003774477.1| metal-dependent hydrolase [Herbaspirillum se... 373 e-101 ref|ZP_02381127.1| amidohydrolase 2 [Burkholderia ubonensis Bu] 350 1e-94 ref|YP_001115832.1| amidohydrolase 2 [Burkholderia vietnamiensis... 348 5e-94 ref|ZP_04947769.1| hypothetical protein BDAG_03751 [Burkholderia... 347 2e-93 >ref|YP_003777873.1| metal-dependent hydrolase [Herbaspirillum seropedicae SmR1] gb|ADJ65965.1| metal-dependent hydrolase of the TIM-barrel fold protein [Herbaspirillum seropedicae SmR1] Length = 299 Score = 485 bits (1249), Expect = e-135, Method: Composition-based stats. Identities = 241/298 (80%), Positives = 264/298 (88%) Query: 10 MPPAPVRRIKGPMRIDAHQHFWRYRSEDYPWIGTGMDLLAQDRLPYQFQPLLDAQGLHAS 69 MP P R K P+RIDAHQHFWRYRSEDYPWIG GMDLLAQDRLPYQFQPLL+AQGLHAS Sbjct: 1 MPTPPAGRCKTPLRIDAHQHFWRYRSEDYPWIGAGMDLLAQDRLPYQFQPLLEAQGLHAS 60 Query: 70 ITVQARPGHEETGFLLEMARRDARIAGVIAWDDIASPQLAERMEQWGRPKMLGLRHPMQD 129 ITVQAR G +ET FLLEMARRD RIAG+I W+D+++P LAE+ME+WGR K+LGLRH +Q+ Sbjct: 61 ITVQARQGRDETAFLLEMARRDKRIAGIIGWEDLSAPDLAEQMERWGRHKLLGLRHQLQE 120 Query: 130 EDDAAQALVQPQFNHGVRWLQERDYVLEVLTHERHLPQLRQLCALHDQHWVVLNHLGKPA 189 DDA L + GVRWLQER Y L+VL HERHLPQLRQ CA HDQHWVVLNHLGKPA Sbjct: 121 HDDANTLLTPAPLDQGVRWLQERSYTLDVLAHERHLPQLRQFCARHDQHWVVLNHLGKPA 180 Query: 190 LKEFRKGRAAYERWRQQLRELAAMPHVACKISGLLTEADWLRGLCTRDFDHIVQCLDTAL 249 LKEFRKGRAA+ERWRQ+LREL AMPHVACKISGLLTEADWLRGLCTRDF+HIVQCLDTAL Sbjct: 181 LKEFRKGRAAFERWRQELRELTAMPHVACKISGLLTEADWLRGLCTRDFEHIVQCLDTAL 240 Query: 250 ELFGPQRLMFGSDWPVCLLAASYARVVAVVREWAARRLSPADQDALWGESSARIYGLM 307 +LFGPQRLMFGSDWPVCLLAASY+RVV VVREW+A RL P+DQDALWGESSARIYGL+ Sbjct: 241 DLFGPQRLMFGSDWPVCLLAASYSRVVTVVREWSATRLCPSDQDALWGESSARIYGLL 298 >ref|YP_003774477.1| metal-dependent hydrolase [Herbaspirillum seropedicae SmR1] gb|ADJ62569.1| metal-dependent hydrolase of the TIM-barrel fold protein [Herbaspirillum seropedicae SmR1] Length = 289 Score = 373 bits (958), Expect = e-101, Method: Composition-based stats. Identities = 184/286 (64%), Positives = 223/286 (77%) Query: 21 PMRIDAHQHFWRYRSEDYPWIGTGMDLLAQDRLPYQFQPLLDAQGLHASITVQARPGHEE 80 P RIDAHQHFW Y+ + YPWIG GM+LLA DR P Q QPLLDA+GL SI VQAR G EE Sbjct: 3 PPRIDAHQHFWFYQPQAYPWIGAGMELLACDRTPGQLQPLLDAEGLGQSIAVQARAGREE 62 Query: 81 TGFLLEMARRDARIAGVIAWDDIASPQLAERMEQWGRPKMLGLRHPMQDEDDAAQALVQP 140 TGFLLE+AR + RIAGV+ W+D+ASP LA +E+WGR K++G RH +QDE D + P Sbjct: 63 TGFLLELARGNRRIAGVVGWEDLASPHLAANVERWGRDKLVGFRHQLQDEADVGGCVDAP 122 Query: 141 QFNHGVRWLQERDYVLEVLTHERHLPQLRQLCALHDQHWVVLNHLGKPALKEFRKGRAAY 200 FN GV WLQ + V +VL + R + +++ CA HD+HW+VL+HLGKPAL EF +G A+ Sbjct: 123 AFNAGVAWLQRQGMVYDVLVYARQMAEVQAFCARHDRHWLVLDHLGKPALTEFGRGDTAF 182 Query: 201 ERWRQQLRELAAMPHVACKISGLLTEADWLRGLCTRDFDHIVQCLDTALELFGPQRLMFG 260 E W++QLR LAA+PHVACK+SGL+TEADW RGL +DFD+I CLDTALE FGPQRLMFG Sbjct: 183 EGWQRQLRALAALPHVACKLSGLVTEADWQRGLRQKDFDNISLCLDTALEAFGPQRLMFG 242 Query: 261 SDWPVCLLAASYARVVAVVREWAARRLSPADQDALWGESSARIYGL 306 SDWPVCLLAASYA+V VVREWAA RLS A+Q+ LWG ++AR YGL Sbjct: 243 SDWPVCLLAASYAKVAGVVREWAATRLSAAEQELLWGATAARCYGL 288 >ref|ZP_02381127.1| amidohydrolase 2 [Burkholderia ubonensis Bu] Length = 285 Score = 350 bits (899), Expect = 1e-94, Method: Composition-based stats. Identities = 174/281 (61%), Positives = 211/281 (75%) Query: 22 MRIDAHQHFWRYRSEDYPWIGTGMDLLAQDRLPYQFQPLLDAQGLHASITVQARPGHEET 81 +RIDAHQHFWRYR+E YPWIG GMD LA+DRLP + PLL A L SI VQAR G +ET Sbjct: 4 LRIDAHQHFWRYRAEHYPWIGAGMDALARDRLPAELHPLLRAHALDRSIAVQARAGRDET 63 Query: 82 GFLLEMARRDARIAGVIAWDDIASPQLAERMEQWGRPKMLGLRHPMQDEDDAAQALVQPQ 141 FLL +AR DARIAGV+ W+DI SP+LA+ + +WGR K+LG RH +QDE D A + P Sbjct: 64 AFLLGLARGDARIAGVVGWEDIGSPRLADHVAEWGRYKLLGFRHQVQDEGDVAAFVSLPA 123 Query: 142 FNHGVRWLQERDYVLEVLTHERHLPQLRQLCALHDQHWVVLNHLGKPALKEFRKGRAAYE 201 F G+ WLQ + YV +VL +R LP +R CA HD HW+VL+HLGKPAL EF + A+ Sbjct: 124 FERGIAWLQAQGYVYDVLVFQRQLPDVRAFCARHDAHWLVLDHLGKPALAEFDRDETAFP 183 Query: 202 RWRQQLRELAAMPHVACKISGLLTEADWLRGLCTRDFDHIVQCLDTALELFGPQRLMFGS 261 RWR LR L A+PHVACK+SGL+TEADW GL +DF HI +CLD ALE+FGPQRLMFGS Sbjct: 184 RWRDALRALGALPHVACKLSGLVTEADWAHGLRPQDFSHIERCLDAALEVFGPQRLMFGS 243 Query: 262 DWPVCLLAASYARVVAVVREWAARRLSPADQDALWGESSAR 302 DWPVCLLAASY +VVA++ WAA RLS +Q A+WG ++AR Sbjct: 244 DWPVCLLAASYDQVVALIERWAATRLSEDEQRAVWGGTAAR 284 >ref|YP_001115832.1| amidohydrolase 2 [Burkholderia vietnamiensis G4] gb|ABO56367.1| amidohydrolase 2 [Burkholderia vietnamiensis G4] Length = 296 Score = 348 bits (894), Expect = 5e-94, Method: Composition-based stats. Identities = 175/285 (61%), Positives = 208/285 (72%) Query: 22 MRIDAHQHFWRYRSEDYPWIGTGMDLLAQDRLPYQFQPLLDAQGLHASITVQARPGHEET 81 +RID+HQHFWRYR+ DYPWIG MD LA+D LP P L A L ASI VQAR G +ET Sbjct: 4 VRIDSHQHFWRYRAADYPWIGPAMDALARDYLPAALWPQLHAHALAASIAVQARAGRDET 63 Query: 82 GFLLEMARRDARIAGVIAWDDIASPQLAERMEQWGRPKMLGLRHPMQDEDDAAQALVQPQ 141 FLLE+AR DARIA V+ W+D+A+P+LAER+ W PK+ G RH +QDE D A + QP Sbjct: 64 AFLLELARDDARIAAVVGWEDLAAPELAERVAAWRSPKLRGFRHQLQDEADVAAFVGQPA 123 Query: 142 FNHGVRWLQERDYVLEVLTHERHLPQLRQLCALHDQHWVVLNHLGKPALKEFRKGRAAYE 201 FN GV WLQ YV +VL ER L +R C HD HW+VL+H GKPAL EF + A+ Sbjct: 124 FNRGVAWLQRHGYVYDVLVFERQLRDVRAFCTRHDAHWLVLDHAGKPALAEFDRDATAFA 183 Query: 202 RWRQQLRELAAMPHVACKISGLLTEADWLRGLCTRDFDHIVQCLDTALELFGPQRLMFGS 261 RWR +LRELAA+PHV CK+SGL+TEADW RGLC D H+ QCLD AL FGPQRLMFGS Sbjct: 184 RWRAELRELAALPHVVCKLSGLVTEADWRRGLCAPDLRHVEQCLDAALAAFGPQRLMFGS 243 Query: 262 DWPVCLLAASYARVVAVVREWAARRLSPADQDALWGESSARIYGL 306 DWPVCLLAASY V ++V WAA RLSPA++DALWG ++AR Y + Sbjct: 244 DWPVCLLAASYDEVASLVERWAAARLSPAERDALWGGTAARCYAV 288 >ref|ZP_04947769.1| hypothetical protein BDAG_03751 [Burkholderia dolosa AUO158] gb|EAY70940.1| hypothetical protein BDAG_03751 [Burkholderia dolosa AUO158] Length = 296 Score = 347 bits (890), Expect = 2e-93, Method: Composition-based stats. Identities = 173/283 (61%), Positives = 207/283 (73%) Query: 22 MRIDAHQHFWRYRSEDYPWIGTGMDLLAQDRLPYQFQPLLDAQGLHASITVQARPGHEET 81 +RID+HQHFWRYR+ DYPWIG GMD+LA+D LP +PL+ AQ L ASI VQAR G +ET Sbjct: 4 LRIDSHQHFWRYRAADYPWIGAGMDVLARDYLPDALRPLMHAQALGASIAVQARAGRDET 63 Query: 82 GFLLEMARRDARIAGVIAWDDIASPQLAERMEQWGRPKMLGLRHPMQDEDDAAQALVQPQ 141 FLLE+AR DARIA V+ W+DI +P LAER+ +W PK+ G RH +QDE D + P Sbjct: 64 AFLLELARDDARIAAVVGWEDIGAPDLAERVAEWRSPKLRGFRHQLQDEADVRAFVADPG 123 Query: 142 FNHGVRWLQERDYVLEVLTHERHLPQLRQLCALHDQHWVVLNHLGKPALKEFRKGRAAYE 201 F GV WLQ YV +VL ER LP +R+ CA HD HW+VL+H GKPAL F + A Sbjct: 124 FERGVAWLQANGYVYDVLVFERQLPDVREFCARHDAHWLVLDHAGKPALAAFDRDDTALA 183 Query: 202 RWRQQLRELAAMPHVACKISGLLTEADWLRGLCTRDFDHIVQCLDTALELFGPQRLMFGS 261 RWR QLREL AMPHVACK+SGL+TEADW RGL +D H+ QCLD AL+ FGPQRLMFGS Sbjct: 184 RWRTQLRELGAMPHVACKLSGLVTEADWRRGLRAQDVRHVEQCLDAALDAFGPQRLMFGS 243 Query: 262 DWPVCLLAASYARVVAVVREWAARRLSPADQDALWGESSARIY 304 DWPVCLLAASY V ++V WA RLS A++ ALWG ++AR Y Sbjct: 244 DWPVCLLAASYDEVASIVERWAESRLSAAERGALWGGTAARCY 286