BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.1757.p (519 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003777809.1| cobalt-zinc-cadmium resistance cation efflux... 517 e-144 ref|YP_986110.1| RND family efflux transporter MFP subunit [Acid... 507 e-141 ref|YP_004124894.1| efflux transporter, rnd family, mfp subunit ... 488 e-135 ref|YP_004753173.1| putative Co/Zn/Cd efflux system membrane fus... 484 e-134 ref|ZP_07673471.1| cation efflux system protein CzcB [Ralstonia ... 484 e-134 >ref|YP_003777809.1| cobalt-zinc-cadmium resistance cation efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] gb|ADJ65901.1| cobalt-zinc-cadmium resistance cation efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 396 Score = 517 bits (1331), Expect = e-144, Method: Composition-based stats. Identities = 293/345 (84%), Positives = 322/345 (93%) Query: 175 SKEEGKIELNKEQMASAGIQVKTAGAMKMNSSLQLPGEIRFNEDRTAHVVPQVAGIVESV 234 S EEGKI LN EQ+A+AGI+VKTAG M +NSS+QLPGEIRFNEDRTAHVVPQVAGIVESV Sbjct: 52 SSEEGKIALNPEQIATAGIEVKTAGPMILNSSVQLPGEIRFNEDRTAHVVPQVAGIVESV 111 Query: 235 SANLGQKVKKGQVLAVVISSAISEQRSELLNSEQRLALAKTAYQREKGLWEQKISAEQDY 294 SA LGQKV+KGQVLAV+ SSA+SEQRSE+LN+EQRLALA+TAYQREK LWEQKISAEQDY Sbjct: 112 SAKLGQKVRKGQVLAVINSSAVSEQRSEMLNAEQRLALARTAYQREKQLWEQKISAEQDY 171 Query: 295 LQAQTTLREAEVAVRNAQQKLRAIGASPTSGGLNRYEIRAPFDGTVVEKHLGLGEAVRED 354 LQAQ LREAEVAVRNAQQKLRAIGA+ +SGGLNRYEIRAPFDG VVEKHLGLGEAV+ED Sbjct: 172 LQAQAALREAEVAVRNAQQKLRAIGATASSGGLNRYEIRAPFDGVVVEKHLGLGEAVKED 231 Query: 355 ANAFLISDLSSVWAEIIVTPKDMQIVRVGEKVVVRATAFDSSTSGQISYVGSLIGEQSRT 414 ANAFLISDLSSVWAEIIV+PKDMQ VR GEKV VRATAFD+S SG+ISYVGSLIGEQSRT Sbjct: 232 ANAFLISDLSSVWAEIIVSPKDMQFVRTGEKVTVRATAFDASASGKISYVGSLIGEQSRT 291 Query: 415 AKAHVVLPNKDDNWRPGLFVNVEVVSDEGQVPVAVAADAIQTVDEKSVVFVRMSGGFAAQ 474 AKAHV+LPN DD+WRPGLFVNVEVVSDE QVPVAV+A+A+QTV+EKSV+FVR+ GGFAAQ Sbjct: 292 AKAHVILPNPDDSWRPGLFVNVEVVSDEAQVPVAVSAEALQTVEEKSVIFVRVPGGFAAQ 351 Query: 475 EVTLGRSNGKFVEIKSGLDAGVEYAATGSFAIKAELGKGTAEHGH 519 +V GRSNGKFVEI+SGL+AG EYAATGSFAIKAELGKG+AEH H Sbjct: 352 QVVTGRSNGKFVEIRSGLNAGDEYAATGSFAIKAELGKGSAEHSH 396 Score = 72.4 bits (176), Expect = 2e-10, Method: Composition-based stats. Identities = 36/53 (67%), Positives = 42/53 (79%), Gaps = 1/53 (1%) Query: 25 MKGGSSAGGGEKSSAHQEDGSHKDNEHHGEDKGSGHKDSAEHADGEHHSTAPS 77 M+ G+ AG EKS AHQE+G HKDNEHHGE+KGS HKD+AEH +GEHH PS Sbjct: 1 MQAGTPAGA-EKSPAHQEEGGHKDNEHHGEEKGSEHKDAAEHGEGEHHDAEPS 52 >ref|YP_986110.1| RND family efflux transporter MFP subunit [Acidovorax sp. JS42] gb|ABM42034.1| efflux transporter, RND family, MFP subunit [Acidovorax sp. JS42] Length = 538 Score = 507 bits (1306), Expect = e-141, Method: Composition-based stats. Identities = 295/527 (55%), Positives = 366/527 (69%), Gaps = 8/527 (1%) Query: 1 MTKKQKLAIAAIIAIAALMSGVTLMKGG-----SSAGGGEKSSAHQEDGSHKDNEHHGED 55 ++KK +AIA ++A+ L GG ++ G G +H E H D EHHG+ Sbjct: 12 ISKKHLIAIAVVLALGVGAGTFILQGGGKPKAATAEGDGHGHGSHTEAKGHGDGEHHGKG 71 Query: 56 KGSGHKDSAEHADGEHHSTAPSKGPHGGSLFKTGASSFEVLLSESDGA--LVVYPYQSDA 113 GH D HADGEHH + +KGPHGG++FK G S E LLSE G L ++ D Sbjct: 72 GEKGHDDDKGHADGEHHEKSEAKGPHGGTVFKEGDFSLEALLSEDGGEPRLRIWLSDKDK 131 Query: 114 PLSPSVVKLAATLNRPGKASEKLEFQVEKDSLKATTPVEEPHLFDIKFDASTPAGSQQFT 173 PL + + AT+ RP +KL F EKDSL + V EPH FDI+ A T F Sbjct: 132 PLPLNAATVTATVTRPTDEKQKLTFAAEKDSLVSREIVAEPHAFDIEIIAQTATEPFMFV 191 Query: 174 LSKEEGKIELNKEQMASAGIQVKTAGAMKMNSSLQLPGEIRFNEDRTAHVVPQVAGIVES 233 +SKEEGKIEL Q+ +A I + A + ++L LPGEIR NEDRT+HVVP++AG+VES Sbjct: 192 MSKEEGKIELTDAQIKAASISLDNAVKANIKTALLLPGEIRLNEDRTSHVVPRLAGVVES 251 Query: 234 VSANLGQKVKKGQVLAVVISSAISEQRSELLNSEQRLALAKTAYQREKGLWEQKISAEQD 293 V+A+LGQ VKKGQVLAV+ S SEQRSEL +++RL LAKT Y+REK LWEQK+SAEQD Sbjct: 252 VNASLGQVVKKGQVLAVIASPTASEQRSELQTAQKRLTLAKTTYEREKKLWEQKVSAEQD 311 Query: 294 YLQAQTTLREAEVAVRNAQQKLRAIGASPTS-GGLNRYEIRAPFDGTVVEKHLGLGEAVR 352 YLQA+ L EAEVAV NA QKL A+G S +S GLNR E+RAPFDG V+EKHL GEAV+ Sbjct: 312 YLQAKQALSEAEVAVANANQKLSAMGLSASSVAGLNRIELRAPFDGIVIEKHLSFGEAVK 371 Query: 353 EDANAFLISDLSSVWAEIIVTPKDMQIVRVGEKVVVRATAFDSSTSGQISYVGSLIGEQS 412 EDA F ISDLS VWAEI V KD+ VRVGEKV ++ATAFD+S +G +++VG+LIGEQ+ Sbjct: 372 EDAAVFTISDLSQVWAEINVPAKDLPAVRVGEKVTIKATAFDASATGTVAFVGALIGEQT 431 Query: 413 RTAKAHVVLPNKDDNWRPGLFVNVEVVSDEGQVPVAVAADAIQTVDEKSVVFVRMSGGFA 472 RTAKA VVL N WRPGLFVNVEVVS+E PV ++ADA+Q V EK VVF+++ GGF Sbjct: 432 RTAKARVVLDNPKGAWRPGLFVNVEVVSEETVAPVTISADAVQNVGEKPVVFLKVDGGFI 491 Query: 473 AQEVTLGRSNGKFVEIKSGLDAGVEYAATGSFAIKAELGKGTAEHGH 519 AQ V LGRS+GK VE+ SGL AG+ YA+TGSF +K+ELGKG+AEH H Sbjct: 492 AQPVKLGRSDGKRVEVLSGLKAGMPYASTGSFVVKSELGKGSAEHTH 538 >ref|YP_004124894.1| efflux transporter, rnd family, mfp subunit [Alicycliphilus denitrificans BC] gb|ADU98006.1| efflux transporter, RND family, MFP subunit [Alicycliphilus denitrificans BC] Length = 530 Score = 488 bits (1256), Expect = e-135, Method: Composition-based stats. Identities = 292/520 (56%), Positives = 366/520 (70%), Gaps = 4/520 (0%) Query: 3 KKQKLAIAAIIAIAALMSGVTLMKGGSSAGGGEKSSAHQEDGSHKDNEHHGEDKGSGHKD 62 KKQ +AI I A+ + L + GE +H E +H D EHHG+ H+D Sbjct: 12 KKQWIAIVVIAAVGIGLGSAILGGKTGKSAEGEGHGSHVEAKAHSDGEHHGKTGADSHED 71 Query: 63 SAEHADGEHHSTAPSKGPHGGSLFKTGASSFEVLLSESDGA--LVVYPYQSDAPLSPSVV 120 HADGEHH + SKGPHGG LFK G E LL+E G L ++ Y+ + L+ S Sbjct: 72 DKGHADGEHHEGS-SKGPHGGKLFKEGDFGLEALLAEDGGEPRLRIWLYEKEKALASSAA 130 Query: 121 KLAATLNRPGKASEKLEFQVEKDSLKATTPVEEPHLFDIKFDASTPAGSQQFTLSKEEGK 180 K++AT+ R + L F +KDSL + V EPH F+I A T F+ S+EEGK Sbjct: 131 KVSATVTRLTGEKQALNFTPDKDSLLSREIVPEPHAFEINIVAQTANAPFTFSFSQEEGK 190 Query: 181 IELNKEQMASAGIQVKTAGAMKMNSSLQLPGEIRFNEDRTAHVVPQVAGIVESVSANLGQ 240 +EL+ Q+ +A I + TAGA + S+LQLPGEIR NEDRT+HVVP++AG+VESV A+LGQ Sbjct: 191 VELSDAQIKAASIGIDTAGAASIKSALQLPGEIRLNEDRTSHVVPRIAGVVESVQASLGQ 250 Query: 241 KVKKGQVLAVVISSAISEQRSELLNSEQRLALAKTAYQREKGLWEQKISAEQDYLQAQTT 300 VKKGQVLAV+ S A SEQRSEL +++RLALA+T ++REK LWEQKISAEQDYLQA Sbjct: 251 TVKKGQVLAVIASPAASEQRSELQTAQKRLALAQTTFEREKRLWEQKISAEQDYLQAGQA 310 Query: 301 LREAEVAVRNAQQKLRAIGASP-TSGGLNRYEIRAPFDGTVVEKHLGLGEAVREDANAFL 359 L EA+VAV NAQQKL A+G +P +SGGLNR+E+RAPFDG V+EKHL LGEAV+EDA F Sbjct: 311 LNEAQVAVANAQQKLSAVGLAPGSSGGLNRFELRAPFDGVVIEKHLSLGEAVKEDAAVFT 370 Query: 360 ISDLSSVWAEIIVTPKDMQIVRVGEKVVVRATAFDSSTSGQISYVGSLIGEQSRTAKAHV 419 +SDL VWAEI V KD+ +VRVGEKV ++ATAFD+S G I++VG+LIGEQ+R AKA V Sbjct: 371 VSDLGQVWAEINVPAKDLPLVRVGEKVSIKATAFDASAQGTITFVGALIGEQTRMAKARV 430 Query: 420 VLPNKDDNWRPGLFVNVEVVSDEGQVPVAVAADAIQTVDEKSVVFVRMSGGFAAQEVTLG 479 VL N WRPGLFVNVEV++ E VPV+VA+DAIQ+V +K VVFV++ GF AQ V LG Sbjct: 431 VLANPQGAWRPGLFVNVEVIASETAVPVSVASDAIQSVGDKPVVFVKVKDGFVAQPVQLG 490 Query: 480 RSNGKFVEIKSGLDAGVEYAATGSFAIKAELGKGTAEHGH 519 RS+GK VE+ GL AG YAA GSF +K+ELGK +AEH H Sbjct: 491 RSDGKRVEVLQGLQAGAAYAAAGSFVVKSELGKASAEHTH 530 >ref|YP_004753173.1| putative Co/Zn/Cd efflux system membrane fusion protein [Collimonas fungivorans Ter331] gb|AEK62350.1| putative Co/Zn/Cd efflux system membrane fusion protein [Collimonas fungivorans Ter331] Length = 526 Score = 484 bits (1246), Expect = e-134, Method: Composition-based stats. Identities = 271/521 (52%), Positives = 361/521 (69%), Gaps = 6/521 (1%) Query: 4 KQKLAIAAIIAIAALMSGVTLMKGGSSAGGGEKSSAHQEDGSHKDNEHHGEDKGSGHKDS 63 K K A I I AL++ + GS+ G + S H+E +H D EHHGE H Sbjct: 7 KSKAIAAGIATIVALVAAGMYLSAGSNGGDKPEESMHREAAAHADTEHHGEKPSEKHGHD 66 Query: 64 AEHADGEHHSTAPSKGPHGGSLFKTGASSFEVLLSESDGA--LVVYPYQSDAPLSPSVVK 121 ++HADGEHH A S GPHGG ++ + EV + E+ G L V+ + PL+P + Sbjct: 67 SQHADGEHHPAA-STGPHGGKMYAKDKLALEVKMLEAAGEARLQVWLFNDGKPLAPEAAR 125 Query: 122 LAATLNRPGKASEKLEFQVEKDSLKATTPVEEPHLFDIKFDASTPAGSQQFTLSKEEGKI 181 L L RP +K+ F EKD LK+T P+ EPH+FD K S Q ++EEGK+ Sbjct: 126 LELVLTRPDGEVQKIGFAPEKDFLKSTLPIAEPHVFDAKLQLQYGNQSMQAGFAQEEGKL 185 Query: 182 ELNKEQMASAGIQVKTAGAMKMNSSLQLPGEIRFNEDRTAHVVPQVAGIVESVSANLGQK 241 EL++ Q+A++G++++ AG K+ +S+ LPGEIRFNED+TAHVVP++ G+VE V+ +LGQ+ Sbjct: 186 ELDQAQVAASGVKLENAGPAKVKTSVTLPGEIRFNEDKTAHVVPRLEGVVEQVAVSLGQQ 245 Query: 242 VKKGQVLAVVISSAISEQRSELLNSEQRLALAKTAYQREKGLWEQKISAEQDYLQAQTTL 301 VKKGQ+LAVV S+ ++EQRSELL +++R A+T Y+REK LWE+KISAEQDY QA+ + Sbjct: 246 VKKGQLLAVVASTGLAEQRSELLAAQKRFTFARTTYEREKKLWEEKISAEQDYQQARQVM 305 Query: 302 REAEVAVRNAQQKLRAIG---ASPTSGGLNRYEIRAPFDGTVVEKHLGLGEAVREDANAF 358 EAE+A++NA+QKL +G + G LNRYEIRAPF+G V EKH+ LGEAV +A+ F Sbjct: 306 SEAEIALQNARQKLNVLGTGVGAGNPGALNRYEIRAPFNGLVTEKHIALGEAVAANASIF 365 Query: 359 LISDLSSVWAEIIVTPKDMQIVRVGEKVVVRATAFDSSTSGQISYVGSLIGEQSRTAKAH 418 ISDLS+VWAEIIV KD+ +VR+GEKV + ATAF+S+ SG ISYVG+L+GEQ+RTAKA Sbjct: 366 TISDLSTVWAEIIVPAKDLNLVRLGEKVKISATAFESTASGSISYVGALLGEQTRTAKAR 425 Query: 419 VVLPNKDDNWRPGLFVNVEVVSDEGQVPVAVAADAIQTVDEKSVVFVRMSGGFAAQEVTL 478 V L N D WRPGLFVNVEVVS + +V VAV AIQ+VD+K VVFVR++ GF Q V+ Sbjct: 426 VTLANPDMAWRPGLFVNVEVVSSDAEVAVAVQPQAIQSVDDKQVVFVRIADGFMVQPVST 485 Query: 479 GRSNGKFVEIKSGLDAGVEYAATGSFAIKAELGKGTAEHGH 519 GR++ + VEI GL G YAA GSF +K+ELGK +AEH H Sbjct: 486 GRADSQHVEIVKGLKPGARYAADGSFVLKSELGKSSAEHAH 526 >ref|ZP_07673471.1| cation efflux system protein CzcB [Ralstonia sp. 5_7_47FAA] gb|EFP68170.1| cation efflux system protein CzcB [Ralstonia sp. 5_7_47FAA] Length = 525 Score = 484 bits (1246), Expect = e-134, Method: Composition-based stats. Identities = 283/525 (53%), Positives = 365/525 (69%), Gaps = 8/525 (1%) Query: 1 MTKKQKLAIAAIIAIAALMSGVTLMKGGSSAG--GGEKSSAHQEDGSHKDNEHHGEDKGS 58 +T KQK AIAA++A + +G L G S+ G +++ H E H+D EHHGE Sbjct: 3 ITNKQKTAIAALVAAGLIATGTVLFLGRSTEGNESAREAAEHAESAGHQDREHHGEPSQG 62 Query: 59 GHKDSAEHADGEHHSTAPSKGPHGGSLFKTGASSFEVLLSESDG--ALVVYPYQSDAPLS 116 H+D+ H D EHH AP KGPHGG+++ G ++ E ++E G L ++ + PL Sbjct: 63 KHEDAVAHGDQEHHD-APKKGPHGGNVYAAGDATVEFGMAEDGGEPKLKLWVVRDGKPLV 121 Query: 117 PSVVKLAATLNRPGKASEKLEFQVEKDSLKATTPVEEPHLFDIKFDASTPAGSQQFTL-- 174 ++ + + R S + F++ KDSL++ + EPH+F P + T+ Sbjct: 122 ANI-SASGAIKRVTGESMPIGFKITKDSLESEAAIAEPHVFGATASVMLPGVQKPMTVAF 180 Query: 175 SKEEGKIELNKEQMASAGIQVKTAGAMKMNSSLQLPGEIRFNEDRTAHVVPQVAGIVESV 234 SKEEGKIEL +Q+A AG+ V+ AGA + S +Q PGEIRFNEDRTAHVVP++AG+VE V Sbjct: 181 SKEEGKIELTVDQIAKAGVGVEVAGAAAVQSGVQFPGEIRFNEDRTAHVVPRLAGVVERV 240 Query: 235 SANLGQKVKKGQVLAVVISSAISEQRSELLNSEQRLALAKTAYQREKGLWEQKISAEQDY 294 AN+G++VK GQVLAV+ S+A+SEQRSELL +++RL+LA+T Y REK LWE+KISAEQDY Sbjct: 241 PANIGEQVKSGQVLAVIASTALSEQRSELLAAQKRLSLAQTTYAREKRLWEEKISAEQDY 300 Query: 295 LQAQTTLREAEVAVRNAQQKLRAIGASPTSGGLNRYEIRAPFDGTVVEKHLGLGEAVRED 354 LQAQT L+EA++AV+NAQQKL AIGA+P + LNR+E+RAPFDG VVEKHL +GEAV+ED Sbjct: 301 LQAQTALQEAQIAVQNAQQKLVAIGAAPATTALNRFELRAPFDGMVVEKHLAIGEAVKED 360 Query: 355 ANAFLISDLSSVWAEIIVTPKDMQIVRVGEKVVVRATAFDSSTSGQISYVGSLIGEQSRT 414 AN F ISDLSSVWAE +V KD+ VRVG++V + +TAFDS +G +SYVGSL+GEQ+RT Sbjct: 361 ANVFTISDLSSVWAEFVVAAKDLGNVRVGQQVTIGSTAFDSKATGSVSYVGSLLGEQTRT 420 Query: 415 AKAHVVLPNKDDNWRPGLFVNVEVVSDEGQVPVAVAADAIQTVDEKSVVFVRMSGGFAAQ 474 AKA V L N WRPGLFV V+V+ QVPVAV ADAIQ V+ K VFV + GGF AQ Sbjct: 421 AKARVTLANPGMAWRPGLFVTVQVLGPAVQVPVAVRADAIQEVNGKPAVFVAVPGGFVAQ 480 Query: 475 EVTLGRSNGKFVEIKSGLDAGVEYAATGSFAIKAELGKGTAEHGH 519 V GRS GK VEI GL AG YA SF +KAELGK +AEH H Sbjct: 481 PVKTGRSTGKVVEITEGLSAGARYATANSFVLKAELGKASAEHEH 525