BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.1681.p (396 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003775689.1| transmembrane protein [Herbaspirillum serope... 688 0.0 ref|YP_001778378.1| AcnD-accessory protein PrpF [Burkholderia ce... 615 e-174 ref|YP_625194.1| PrpF, AcnD-accessory [Burkholderia cenocepacia ... 615 e-174 ref|ZP_02377283.1| hypothetical protein BuboB_06141 [Burkholderi... 614 e-173 ref|YP_370905.1| methylitaconate delta2-delta3-isomerase [Burkho... 612 e-173 >ref|YP_003775689.1| transmembrane protein [Herbaspirillum seropedicae SmR1] gb|ADJ63781.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 396 Score = 688 bits (1776), Expect = 0.0, Method: Composition-based stats. Identities = 383/396 (96%), Positives = 392/396 (98%) Query: 1 MSQIPQIRIAATYLRGGTSKGVFFKLDDLPEAARVPGPARDQLLQRVIGSPDPYGKQIDG 60 MSQ+PQIRI ATYLRGGTSKGVFFKLDDLPEAARVPGPARD+LLQRVIGSPDPYGKQIDG Sbjct: 1 MSQVPQIRIPATYLRGGTSKGVFFKLDDLPEAARVPGPARDKLLQRVIGSPDPYGKQIDG 60 Query: 61 MGGATSSTSKTVIISRSERPGHDVDYLFGQVSIDKDFVDWSGNCGNLSAAVGPFAISAGL 120 MGGATSSTSKTVIISRS RPGHDVDYLFGQVSID+DFVDWSGNCGNLSAAVGPFAI+AGL Sbjct: 61 MGGATSSTSKTVIISRSVRPGHDVDYLFGQVSIDQDFVDWSGNCGNLSAAVGPFAIAAGL 120 Query: 121 VDPSKLPQDGVAVITIWQANIGKTIVAHVPIRGGQVQETGDFELDGVTFPAAEVQLEFMD 180 VDPS+LPQDGVAVITIWQANIGKTIVAHVP+RGGQVQETGDFELDGVTFPAAEVQLEFMD Sbjct: 121 VDPSRLPQDGVAVITIWQANIGKTIVAHVPMRGGQVQETGDFELDGVTFPAAEVQLEFMD 180 Query: 181 PAAEEEGAGGAMFPTGNVVDTLEVPGVGSFQATMINAGIPTIFLNADEIGYTGTELQDAI 240 PAAEEEGAGGAMFPTGNVVDTLEVPGVGSFQATMINAGIPTIFL A+EIGYTGTELQDAI Sbjct: 181 PAAEEEGAGGAMFPTGNVVDTLEVPGVGSFQATMINAGIPTIFLKAEEIGYTGTELQDAI 240 Query: 241 NSDPAALARFETIRAHGAIRMGLISKVEEAATRQHTPKVAFVAPPKEYVSSSGKTVTVQD 300 NSDPAALARFETIRAHGA+RMGLISKVEEAATRQHTPKVAFVA P+EYVSSSGKTVTVQD Sbjct: 241 NSDPAALARFETIRAHGAMRMGLISKVEEAATRQHTPKVAFVAAPREYVSSSGKTVTVQD 300 Query: 301 TDVLVRAMSMGKLHHAMMGTAAVAIGTAAAIPGTLVNLAAGGGERNSVRFGHPSGTLRVG 360 TDVLVRAMSMGKLHHAMMGTAAVAIGTAAAIPGTLVNLAAGGGERNSVRFGHPSGTLRVG Sbjct: 301 TDVLVRAMSMGKLHHAMMGTAAVAIGTAAAIPGTLVNLAAGGGERNSVRFGHPSGTLRVG 360 Query: 361 AEAKLEDGQWVVTKAIMSRSARVLMEGWVRVPGDAF 396 AEAKLEDGQWVVTKAIMSRSARVLMEGWVRVPGDAF Sbjct: 361 AEAKLEDGQWVVTKAIMSRSARVLMEGWVRVPGDAF 396 >ref|YP_001778378.1| AcnD-accessory protein PrpF [Burkholderia cenocepacia MC0-3] gb|ACA93888.1| AcnD-accessory protein PrpF [Burkholderia cenocepacia MC0-3] Length = 396 Score = 615 bits (1587), Expect = e-174, Method: Composition-based stats. Identities = 327/396 (82%), Positives = 362/396 (91%) Query: 1 MSQIPQIRIAATYLRGGTSKGVFFKLDDLPEAARVPGPARDQLLQRVIGSPDPYGKQIDG 60 M+ IPQIRI ATY+RGGTSKGVFF+L DLP+AA+ PG ARD LL RVIGSPDPYGKQIDG Sbjct: 1 MAHIPQIRIPATYMRGGTSKGVFFRLQDLPDAAQAPGAARDALLLRVIGSPDPYGKQIDG 60 Query: 61 MGGATSSTSKTVIISRSERPGHDVDYLFGQVSIDKDFVDWSGNCGNLSAAVGPFAISAGL 120 MGGATSSTSKTVI+S+S RPGHDVDYLFGQV+IDK FVDW+GNCGNLSAAVGPFAIS GL Sbjct: 61 MGGATSSTSKTVIVSKSTRPGHDVDYLFGQVAIDKAFVDWTGNCGNLSAAVGPFAISGGL 120 Query: 121 VDPSKLPQDGVAVITIWQANIGKTIVAHVPIRGGQVQETGDFELDGVTFPAAEVQLEFMD 180 VDP+++P+DGVA + IWQANIGKTI+AHVPI G VQETGDFELDGVTFPAAEVQLEFMD Sbjct: 121 VDPARVPRDGVATVRIWQANIGKTIIAHVPITNGAVQETGDFELDGVTFPAAEVQLEFMD 180 Query: 181 PAAEEEGAGGAMFPTGNVVDTLEVPGVGSFQATMINAGIPTIFLNADEIGYTGTELQDAI 240 PAAEEEGAGG+MFPTGN+VD L VPG+G+ +ATMINAGIPTIF++A+ IGYTGTELQDAI Sbjct: 181 PAAEEEGAGGSMFPTGNLVDDLAVPGIGTLKATMINAGIPTIFVDAEAIGYTGTELQDAI 240 Query: 241 NSDPAALARFETIRAHGAIRMGLISKVEEAATRQHTPKVAFVAPPKEYVSSSGKTVTVQD 300 N DP AL +FETIRAHGA+RMGLI ++E ATRQHTPK+AFVA P +YV+SSGK V D Sbjct: 241 NGDPKALEKFETIRAHGALRMGLIDTLDEIATRQHTPKIAFVAKPADYVASSGKRVNAGD 300 Query: 301 TDVLVRAMSMGKLHHAMMGTAAVAIGTAAAIPGTLVNLAAGGGERNSVRFGHPSGTLRVG 360 D+LVRAMSMGKLHHAMMGTAAVAIGTAAAIPGTLVNLAAGGGERN+VRFGHPSGTLRVG Sbjct: 301 VDLLVRAMSMGKLHHAMMGTAAVAIGTAAAIPGTLVNLAAGGGERNAVRFGHPSGTLRVG 360 Query: 361 AEAKLEDGQWVVTKAIMSRSARVLMEGWVRVPGDAF 396 AEA+ E+G+W VTKAIMSRSARVLMEGWVRVPGDAF Sbjct: 361 AEARRENGEWTVTKAIMSRSARVLMEGWVRVPGDAF 396 >ref|YP_625194.1| PrpF, AcnD-accessory [Burkholderia cenocepacia AU 1054] ref|YP_839140.1| putative AcnD-accessory protein PrpF [Burkholderia cenocepacia HI2424] gb|ABF80221.1| 2-methylaconitate cis-trans isomerase [Burkholderia cenocepacia AU 1054] gb|ABK12247.1| 2-methylaconitate cis-trans isomerase [Burkholderia cenocepacia HI2424] Length = 396 Score = 615 bits (1586), Expect = e-174, Method: Composition-based stats. Identities = 327/396 (82%), Positives = 362/396 (91%) Query: 1 MSQIPQIRIAATYLRGGTSKGVFFKLDDLPEAARVPGPARDQLLQRVIGSPDPYGKQIDG 60 M+ IPQIRI ATY+RGGTSKGVFF+L DLP+AA+ PG ARD LL RVIGSPDPYGKQIDG Sbjct: 1 MAHIPQIRIPATYIRGGTSKGVFFRLQDLPDAAQAPGAARDALLLRVIGSPDPYGKQIDG 60 Query: 61 MGGATSSTSKTVIISRSERPGHDVDYLFGQVSIDKDFVDWSGNCGNLSAAVGPFAISAGL 120 MGGATSSTSKTVI+S+S RPGHDVDYLFGQV+IDK FVDW+GNCGNLSAAVGPFAIS GL Sbjct: 61 MGGATSSTSKTVIVSKSTRPGHDVDYLFGQVAIDKAFVDWTGNCGNLSAAVGPFAISGGL 120 Query: 121 VDPSKLPQDGVAVITIWQANIGKTIVAHVPIRGGQVQETGDFELDGVTFPAAEVQLEFMD 180 VDP+++P+DGVA + IWQANIGKTI+AHVPI G VQETGDFELDGVTFPAAEVQLEFMD Sbjct: 121 VDPARVPRDGVATVRIWQANIGKTIIAHVPITNGAVQETGDFELDGVTFPAAEVQLEFMD 180 Query: 181 PAAEEEGAGGAMFPTGNVVDTLEVPGVGSFQATMINAGIPTIFLNADEIGYTGTELQDAI 240 PAAEEEGAGG+MFPTGN+VD L VPG+G+ +ATMINAGIPTIF++A+ IGYTGTELQDAI Sbjct: 181 PAAEEEGAGGSMFPTGNLVDDLAVPGIGTLKATMINAGIPTIFVDAEAIGYTGTELQDAI 240 Query: 241 NSDPAALARFETIRAHGAIRMGLISKVEEAATRQHTPKVAFVAPPKEYVSSSGKTVTVQD 300 N DP AL +FETIRAHGA+RMGLI ++E ATRQHTPK+AFVA P +YV+SSGK V D Sbjct: 241 NGDPKALEKFETIRAHGALRMGLIDTLDEIATRQHTPKIAFVAKPADYVASSGKRVNAGD 300 Query: 301 TDVLVRAMSMGKLHHAMMGTAAVAIGTAAAIPGTLVNLAAGGGERNSVRFGHPSGTLRVG 360 D+LVRAMSMGKLHHAMMGTAAVAIGTAAAIPGTLVNLAAGGGERN+VRFGHPSGTLRVG Sbjct: 301 VDLLVRAMSMGKLHHAMMGTAAVAIGTAAAIPGTLVNLAAGGGERNAVRFGHPSGTLRVG 360 Query: 361 AEAKLEDGQWVVTKAIMSRSARVLMEGWVRVPGDAF 396 AEA+ E+G+W VTKAIMSRSARVLMEGWVRVPGDAF Sbjct: 361 AEARRENGEWTVTKAIMSRSARVLMEGWVRVPGDAF 396 >ref|ZP_02377283.1| hypothetical protein BuboB_06141 [Burkholderia ubonensis Bu] Length = 396 Score = 614 bits (1583), Expect = e-173, Method: Composition-based stats. Identities = 329/396 (83%), Positives = 359/396 (90%) Query: 1 MSQIPQIRIAATYLRGGTSKGVFFKLDDLPEAARVPGPARDQLLQRVIGSPDPYGKQIDG 60 M+ IPQIRI ATYLRGGTSKGVFF+L DLPEAA+ PG ARD LL RVIGSPDPYGKQIDG Sbjct: 1 MAHIPQIRIPATYLRGGTSKGVFFRLQDLPEAAQAPGAARDALLLRVIGSPDPYGKQIDG 60 Query: 61 MGGATSSTSKTVIISRSERPGHDVDYLFGQVSIDKDFVDWSGNCGNLSAAVGPFAISAGL 120 MGGATSSTSKTVI+S+S RPGHDVDYLFGQVSIDK FVDW+GNCGNLSAAVGPFAIS GL Sbjct: 61 MGGATSSTSKTVIVSKSTRPGHDVDYLFGQVSIDKAFVDWTGNCGNLSAAVGPFAISGGL 120 Query: 121 VDPSKLPQDGVAVITIWQANIGKTIVAHVPIRGGQVQETGDFELDGVTFPAAEVQLEFMD 180 VDPS++P+DG+A + IWQANIGKTIVAHVPI G VQETGDFELDGVTFPAAEVQLEFMD Sbjct: 121 VDPSRVPRDGIATVRIWQANIGKTIVAHVPITNGAVQETGDFELDGVTFPAAEVQLEFMD 180 Query: 181 PAAEEEGAGGAMFPTGNVVDTLEVPGVGSFQATMINAGIPTIFLNADEIGYTGTELQDAI 240 PAA+EEGAGGAMFPTGN+VD L VPG+G+ +ATMINAGIPTIF+ A+ IGYTGTELQDAI Sbjct: 181 PAADEEGAGGAMFPTGNLVDDLAVPGIGTLKATMINAGIPTIFVEAEAIGYTGTELQDAI 240 Query: 241 NSDPAALARFETIRAHGAIRMGLISKVEEAATRQHTPKVAFVAPPKEYVSSSGKTVTVQD 300 N D AL +FETIRAHGA+RMGLI ++E ATRQHTPK+AFVA P +YV+SSGK V D Sbjct: 241 NGDAKALEKFETIRAHGALRMGLIDTLDEIATRQHTPKIAFVAKPADYVASSGKRVAAAD 300 Query: 301 TDVLVRAMSMGKLHHAMMGTAAVAIGTAAAIPGTLVNLAAGGGERNSVRFGHPSGTLRVG 360 D+LVRAMSMGKLHHAMMGTAAVAIGTAAAIPGTLVNLAAGG ER +VRFGHPSGTLRVG Sbjct: 301 VDLLVRAMSMGKLHHAMMGTAAVAIGTAAAIPGTLVNLAAGGSERQAVRFGHPSGTLRVG 360 Query: 361 AEAKLEDGQWVVTKAIMSRSARVLMEGWVRVPGDAF 396 A+AKLE+G+W VTKAIMSRSARVLMEGWVRVPGDAF Sbjct: 361 AQAKLENGEWTVTKAIMSRSARVLMEGWVRVPGDAF 396 >ref|YP_370905.1| methylitaconate delta2-delta3-isomerase [Burkholderia sp. 383] gb|ABB10261.1| 2-methylaconitate cis-trans isomerase [Burkholderia sp. 383] Length = 396 Score = 612 bits (1578), Expect = e-173, Method: Composition-based stats. Identities = 328/396 (82%), Positives = 360/396 (90%) Query: 1 MSQIPQIRIAATYLRGGTSKGVFFKLDDLPEAARVPGPARDQLLQRVIGSPDPYGKQIDG 60 M+ PQIRI ATY+RGGTSKGVFF+L DLP+AA+ PG ARD LL RVIGSPDPYGKQIDG Sbjct: 1 MAHTPQIRIPATYIRGGTSKGVFFRLQDLPDAAQAPGAARDALLLRVIGSPDPYGKQIDG 60 Query: 61 MGGATSSTSKTVIISRSERPGHDVDYLFGQVSIDKDFVDWSGNCGNLSAAVGPFAISAGL 120 MGGATSSTSKTVI+S+S RPGHDVDYLFGQVSIDK FVDW+GNCGNLSAAVGPFAIS GL Sbjct: 61 MGGATSSTSKTVIVSKSTRPGHDVDYLFGQVSIDKAFVDWTGNCGNLSAAVGPFAISGGL 120 Query: 121 VDPSKLPQDGVAVITIWQANIGKTIVAHVPIRGGQVQETGDFELDGVTFPAAEVQLEFMD 180 VDP+++P+DGVA + IWQANIGKTIVAHVP+ G VQETGDFELDGVTFPAAEVQLEFMD Sbjct: 121 VDPARVPRDGVATVRIWQANIGKTIVAHVPVTNGAVQETGDFELDGVTFPAAEVQLEFMD 180 Query: 181 PAAEEEGAGGAMFPTGNVVDTLEVPGVGSFQATMINAGIPTIFLNADEIGYTGTELQDAI 240 PAAEEEGAGG+MFPTGN+VD L VPGVG+ +ATMINAGIPTIF+ A+ IGYTGTELQDAI Sbjct: 181 PAAEEEGAGGSMFPTGNLVDDLAVPGVGTLKATMINAGIPTIFVEAEAIGYTGTELQDAI 240 Query: 241 NSDPAALARFETIRAHGAIRMGLISKVEEAATRQHTPKVAFVAPPKEYVSSSGKTVTVQD 300 N D AL +FETIRAHGA+RMGLI ++E ATRQHTPK+AFVA P +YV+SSGK V D Sbjct: 241 NGDAKALEKFETIRAHGALRMGLIDTLDEIATRQHTPKIAFVAKPADYVASSGKRVNAGD 300 Query: 301 TDVLVRAMSMGKLHHAMMGTAAVAIGTAAAIPGTLVNLAAGGGERNSVRFGHPSGTLRVG 360 D+LVRAMSMGKLHHAMMGTAAVAIGTAAAIPGTLVNLAAGGGERN+VRFGHPSGTLRVG Sbjct: 301 VDLLVRAMSMGKLHHAMMGTAAVAIGTAAAIPGTLVNLAAGGGERNAVRFGHPSGTLRVG 360 Query: 361 AEAKLEDGQWVVTKAIMSRSARVLMEGWVRVPGDAF 396 AEA+LE+G+W VTKAIMSRSARVLMEGWVRVPGDAF Sbjct: 361 AEAQLENGEWTVTKAIMSRSARVLMEGWVRVPGDAF 396