BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.1501.p (309 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003775531.1| methylglyoxal synthase [Herbaspirillum serop... 556 e-156 ref|YP_004752235.1| methylglyoxal synthase [Collimonas fungivora... 448 e-124 ref|YP_001352759.1| hypothetical protein mma_1069 [Janthinobacte... 442 e-122 ref|YP_001156407.1| hypothetical protein Pnuc_1629 [Polynucleoba... 397 e-108 ref|YP_786041.1| methylglyoxal synthase [Bordetella avium 197N] ... 309 4e-82 >ref|YP_003775531.1| methylglyoxal synthase [Herbaspirillum seropedicae SmR1] gb|ADJ63623.1| methylglyoxal synthase protein [Herbaspirillum seropedicae SmR1] Length = 295 Score = 556 bits (1432), Expect = e-156, Method: Composition-based stats. Identities = 276/294 (93%), Positives = 283/294 (96%) Query: 15 MSSIAPRYRIGLIANRAHQDASDSALVQLLTGSRHRIESLQPELIVVGRTLDAIGQMNLL 74 MSSIAPRYRIGLIANRAHQDA DSALVQLLTGSRHRIESLQPELIVVGRTLDAIGQMNLL Sbjct: 1 MSSIAPRYRIGLIANRAHQDAPDSALVQLLTGSRHRIESLQPELIVVGRTLDAIGQMNLL 60 Query: 75 PGYEHLVRFPYGRHGGLMKLVSRTVDPDPARTLDAVIYLIDPVDPSSTFPEAVALKRQCV 134 PGYEHLVRFPYGRHGGLMKLVSRTVD DP RTLDAVIYLIDPVDPSSTFPEAVALKRQCV Sbjct: 61 PGYEHLVRFPYGRHGGLMKLVSRTVDEDPERTLDAVIYLIDPVDPSSTFPEAVALKRQCV 120 Query: 135 IHGKPFLSTLAGAQEWFELEALANGAAPDSFLDDRFHLKDEGIALIAHDAMKTRMLELAE 194 IHGKPFLSTLAGAQEWFELEALANGAAPD+ LDDRF L EGIALIAHDAMK+RMLELAE Sbjct: 121 IHGKPFLSTLAGAQEWFELEALANGAAPDASLDDRFRLGQEGIALIAHDAMKSRMLELAE 180 Query: 195 KHFDVLDQFAFRCATGTTGGLLNQLAVKMKGEAGRNWVKPFLSGPLGGDAQIAELILERQ 254 KHF VLDQFAFRCATGTTGGLLNQLA+K+KGEAGR+WVKPFLSGPLGGDAQIAELILE+Q Sbjct: 181 KHFAVLDQFAFRCATGTTGGLLNQLAMKIKGEAGRHWVKPFLSGPLGGDAQIAELILEQQ 240 Query: 255 CRRVLFLEDPHVARQHEADIQLLERAARTVTGYASCMSDVKWGDRWLSLIKARA 308 CRRVLFLEDPHVARQHEADIQLLERAARTVT YASCMSDVKWGDRWL+LI RA Sbjct: 241 CRRVLFLEDPHVARQHEADIQLLERAARTVTAYASCMSDVKWGDRWLTLIGQRA 294 >ref|YP_004752235.1| methylglyoxal synthase [Collimonas fungivorans Ter331] gb|AEK61412.1| Methylglyoxal synthase [Collimonas fungivorans Ter331] Length = 333 Score = 448 bits (1152), Expect = e-124, Method: Composition-based stats. Identities = 231/296 (78%), Positives = 257/296 (86%), Gaps = 4/296 (1%) Query: 16 SSIAPRYRIGLIANRAHQDASDSALVQLLTGSRHRIES-LQPELIVVGRTLDAIGQMNLL 74 +S AP +RIGL ANR+HQ DSAL QLLTGSRH IE L+P+L+VVGRTLDA+ Q+ LL Sbjct: 29 ASSAP-FRIGLAANRSHQQGPDSALAQLLTGSRHSIEQQLKPQLVVVGRTLDAMQQLGLL 87 Query: 75 PGYEHLVRFPYGRHGGLMKLVSRTVDPDPARTLDAVIYLIDPVDPSSTFPEAVALKRQCV 134 Y H+ RFPYGRHGGLMKLVSR VD DPARTLDAVIYL+DPVDPSSTFPEAVALKRQCV Sbjct: 88 EHYPHIERFPYGRHGGLMKLVSRVVDADPARTLDAVIYLMDPVDPSSTFPEAVALKRQCV 147 Query: 135 IHGKPFLSTLAGAQEWFELEALANGAAPDSFLDDRFHLKDEGIALIAHDAMKTRMLELAE 194 IHGKPFLSTLAGA+EW ELEA+A GAAP+ LD F L EGIALIAHDAMK RML++AE Sbjct: 148 IHGKPFLSTLAGAREWLELEAMAAGAAPNPALDPTFELAQEGIALIAHDAMKDRMLDIAE 207 Query: 195 KHFDVLDQFAFRCATGTTGGLLNQLAVKMKGE-AGRNWVKPFLSGPLGGDAQIAELILER 253 +HF +LDQFAFRCATGTTG LLN+LA K+KGE AGRNWVKPFLSGPLGGDAQIAELIL+R Sbjct: 208 RHFALLDQFAFRCATGTTGSLLNKLAQKIKGEQAGRNWVKPFLSGPLGGDAQIAELILDR 267 Query: 254 -QCRRVLFLEDPHVARQHEADIQLLERAARTVTGYASCMSDVKWGDRWLSLIKARA 308 QCRRVLFLEDPHVARQHEADIQLLERAARTV YA C+SD + G+RWL+L++ RA Sbjct: 268 QQCRRVLFLEDPHVARQHEADIQLLERAARTVPDYALCISDAECGERWLTLLQQRA 323 >ref|YP_001352759.1| hypothetical protein mma_1069 [Janthinobacterium sp. Marseille] gb|ABR88830.1| Uncharacterized conserved protein [Janthinobacterium sp. Marseille] Length = 315 Score = 442 bits (1136), Expect = e-122, Method: Composition-based stats. Identities = 220/303 (72%), Positives = 254/303 (83%), Gaps = 3/303 (0%) Query: 8 PTRLLAPMSSIAPRYRIGLIANRAHQDASDSALVQLLTGSRHRIES-LQPELIVVGRTLD 66 P PM++ ++RIGL +R HQDA +S L +LL GSR IE LQP+LIVVGRTLD Sbjct: 7 PVFTFIPMTTTPHQFRIGLATSRTHQDAPNSVLARLLNGSRIAIEQHLQPQLIVVGRTLD 66 Query: 67 AIGQMNLLPGYEHLVRFPYGRHGGLMKLVSRTVDPDPARTLDAVIYLIDPVDPSSTFPEA 126 A+ + LL Y H+ RFPYGRHGGLMKLVSR VD DPAR LDAV+YL+DPVDPSS FPEA Sbjct: 67 AMDHLGLLKNYPHIERFPYGRHGGLMKLVSRVVDTDPARRLDAVMYLMDPVDPSSIFPEA 126 Query: 127 VALKRQCVIHGKPFLSTLAGAQEWFELEALANGAAPDSFLDDRFHLKDEGIALIAHDAMK 186 VALKRQCVIHGKPFLSTLAGA+EW ELE++A GAAP+ LD+ F LK+EGIA+IAHDAMK Sbjct: 127 VALKRQCVIHGKPFLSTLAGAREWLELESIAIGAAPNPELDETFDLKNEGIAMIAHDAMK 186 Query: 187 TRMLELAEKHFDVLDQFAFRCATGTTGGLLNQLAVKMKG-EAGRNWVKPFLSGPLGGDAQ 245 RMLE+AE+HFD+LDQF FRCATGTTG LLN+LA K+KG EAGRNWVKP+LSGP+GGDAQ Sbjct: 187 GRMLEIAERHFDLLDQFGFRCATGTTGSLLNKLAQKIKGEEAGRNWVKPYLSGPMGGDAQ 246 Query: 246 IAELILER-QCRRVLFLEDPHVARQHEADIQLLERAARTVTGYASCMSDVKWGDRWLSLI 304 IAELIL++ QCRRVLFLEDPHVARQHEADIQLLERAARTVT YA C+S+ G+RWL L+ Sbjct: 247 IAELILDKDQCRRVLFLEDPHVARQHEADIQLLERAARTVTDYALCISNENCGERWLDLL 306 Query: 305 KAR 307 + R Sbjct: 307 QKR 309 >ref|YP_001156407.1| hypothetical protein Pnuc_1629 [Polynucleobacter necessarius subsp. asymbioticus QLW-P1DMWA-1] gb|ABP34843.1| conserved hypothetical protein [Polynucleobacter necessarius subsp. asymbioticus QLW-P1DMWA-1] Length = 296 Score = 397 bits (1020), Expect = e-108, Method: Composition-based stats. Identities = 197/296 (66%), Positives = 232/296 (78%), Gaps = 2/296 (0%) Query: 15 MSSIAPRYRIGLIANRAHQDASDSALVQLLTGSRHRI-ESLQPELIVVGRTLDAIGQMNL 73 M + P YRIGL+ANRAHQD+ D+ L+QLL GS+ I E+L+P VVGRT+DA+ +L Sbjct: 1 MVNEVPNYRIGLVANRAHQDSEDATLIQLLNGSKASITETLKPHFSVVGRTMDAMKIYDL 60 Query: 74 LPGYEHLVRFPYGRHGGLMKLVSRTVDPDPARTLDAVIYLIDPVDPSSTFPEAVALKRQC 133 LP Y + R PYG GGLM+LVSR VDPDP + L+A+IYL+DPVDPSS FPEAVALKRQC Sbjct: 61 LPDYADIERLPYGHDGGLMRLVSRVVDPDPNKALNAIIYLMDPVDPSSAFPEAVALKRQC 120 Query: 134 VIHGKPFLSTLAGAQEWFELEALANGAAPDSFLDDRFHLKDEGIALIAHDAMKTRMLELA 193 VIHGKPFLSTLAGA+EW ELE++ LD F +++GIALIAHDAMK ML LA Sbjct: 121 VIHGKPFLSTLAGAREWIELESMNLTGKKTKDLDPYFAYENQGIALIAHDAMKPEMLRLA 180 Query: 194 EKHFDVLDQFAFRCATGTTGGLLNQLAVKMKGEA-GRNWVKPFLSGPLGGDAQIAELILE 252 EKHFD+LD+F R ATGTTGGLLN+LA ++KG G WV P LSGP+GGDAQIAEL+L+ Sbjct: 181 EKHFDLLDRFKVRYATGTTGGLLNELAKQIKGSKDGEGWVTPCLSGPMGGDAQIAELVLD 240 Query: 253 RQCRRVLFLEDPHVARQHEADIQLLERAARTVTGYASCMSDVKWGDRWLSLIKARA 308 R+C R+LFLEDPHVARQHEADIQLLERAAR VT +ASC SD K DRWLSL+ RA Sbjct: 241 RKCGRILFLEDPHVARQHEADIQLLERAARVVTDFASCNSDNKSADRWLSLLSQRA 296 >ref|YP_786041.1| methylglyoxal synthase [Bordetella avium 197N] emb|CAJ49128.1| putative methylglyoxal synthase [Bordetella avium 197N] Length = 298 Score = 309 bits (791), Expect = 4e-82, Method: Composition-based stats. Identities = 161/287 (56%), Positives = 194/287 (67%), Gaps = 1/287 (0%) Query: 20 PRYRIGLIANRAHQDASDSALVQLLTGSRHRIESLQPELIVVGRTLDAIGQMNLLPGYEH 79 P R GL ANR H + D+AL L S I + EL VGRT DAI + +L GY Sbjct: 4 PSLRFGLAANRLHHETPDAALFNWLRASGASIREMGIELHTVGRTHDAIVREGMLQGYRG 63 Query: 80 LVRFPYGRHGGLMKLVSR-TVDPDPARTLDAVIYLIDPVDPSSTFPEAVALKRQCVIHGK 138 L+R+PYGR GGLMKLV+R T D D IYLIDPVDPSS FPEA+ALKRQC+ HG+ Sbjct: 64 LIRYPYGREGGLMKLVARVTQGRDGQAPFDGAIYLIDPVDPSSIFPEALALKRQCITHGR 123 Query: 139 PFLSTLAGAQEWFELEALANGAAPDSFLDDRFHLKDEGIALIAHDAMKTRMLELAEKHFD 198 PF+STLAGA EW E+E + G APD L+ F + + +A+IAHDA+K M+ A+ +FD Sbjct: 124 PFISTLAGAIEWIEIERVLAGLAPDPALNRLFRFEGQTLAMIAHDALKDEMMAFADANFD 183 Query: 199 VLDQFAFRCATGTTGGLLNQLAVKMKGEAGRNWVKPFLSGPLGGDAQIAELILERQCRRV 258 VL QFA R ATGTTGG LN+LA + WV +LSGPLGGDAQIAEL+LE QC+RV Sbjct: 184 VLSQFARRVATGTTGGRLNELAWSRGWPKDQPWVTRYLSGPLGGDAQIAELVLEHQCQRV 243 Query: 259 LFLEDPHVARQHEADIQLLERAARTVTGYASCMSDVKWGDRWLSLIK 305 +F EDPHVARQHEADIQLLERA R VT A+C + RW ++ Sbjct: 244 IFFEDPHVARQHEADIQLLERAVRVVTESATCATSPSVARRWADAVR 290