BLASTP 2.2.17 [Aug-26-2007]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for composition-based statistics:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= 0001.1496.p         (578 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           15,459,386 sequences; 5,307,515,862 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|YP_003775527.1| chloride channel protein [Herbaspirillum ser...   954   0.0  
ref|YP_004752221.1| putative chloride channel family protein [Co...   703   0.0  
ref|ZP_06842894.1| Cl- channel voltage-gated family protein [Bur...   675   0.0  
ref|YP_559043.1| putative voltage-gated ClC-type chloride channe...   672   0.0  
ref|ZP_02355528.1| putative voltage-gated ClC-type chloride chan...   657   0.0  

>ref|YP_003775527.1| chloride channel protein [Herbaspirillum seropedicae SmR1]
 gb|ADJ63619.1| chloride channel protein [Herbaspirillum seropedicae SmR1]
          Length = 555

 Score =  954 bits (2466), Expect = 0.0,   Method: Composition-based stats.
 Identities = 523/555 (94%), Positives = 541/555 (97%)

Query: 24  MLLWGALAGFVGALATIAFRECIALLQIWLTGHDGSFVDIAKGLSWPVRVLLPTCGGVIA 83
           MLLWGALAGFVGALATIAFRECIALLQIW+TGHDGSFVDIAKGL W  RVLLPTCGGV+A
Sbjct: 1   MLLWGALAGFVGALATIAFRECIALLQIWMTGHDGSFVDIAKGLPWYTRVLLPTCGGVVA 60

Query: 84  GLFLVWAKRTPAGNGGDYMEAVAIGDGAIPVRNTLLRSISSLASIASGGSIGREGPMVQL 143
           GLFLVWAKRTPAGNGGDYMEAVAIGDGAIPVRNTLLRSISSLASIASGGSIGREGPMVQL
Sbjct: 61  GLFLVWAKRTPAGNGGDYMEAVAIGDGAIPVRNTLLRSISSLASIASGGSIGREGPMVQL 120

Query: 144 SALCASLVGKFSHFPSSRLRLLVACGAAAGITSAYNAPIAGAFFITEIVLGSLVMESFGP 203
           SALCASLVGKFSHFPSSRLRLLVACGAAAGITSAYNAPIAGAFFITEIVLGSLVMESFGP
Sbjct: 121 SALCASLVGKFSHFPSSRLRLLVACGAAAGITSAYNAPIAGAFFITEIVLGSLVMESFGP 180

Query: 204 VVVASVVANITMRELPGYRAAYEMPYFPEIGGWEVLLFLVLGVLAGILAPQFLRVLELGK 263
           VVVASVVANITMRELPGYRAAYEMPYFPEIGGWEV+LFLVLGVLAG+LAPQFLRVLELGK
Sbjct: 181 VVVASVVANITMRELPGYRAAYEMPYFPEIGGWEVMLFLVLGVLAGVLAPQFLRVLELGK 240

Query: 264 QGFGKFKLPLPVRLGVGGLLVGLISIEVPEVWGNGYSLVNSLLHTTWLWQAVLMVLVIKV 323
            GFGK +LPLPVRLGVGGLLVGLIS++VPEVWGNGYSLVNSLLHT+W+WQAVLMVLV+KV
Sbjct: 241 HGFGKLRLPLPVRLGVGGLLVGLISVQVPEVWGNGYSLVNSLLHTSWVWQAVLMVLVVKV 300

Query: 324 LATAITVGSGAVGGIFTPTLFVGAAVGYLFGDTAQALLPFHMSQPFAYAMVGMGAFLAAA 383
           LAT+ITVGSGAVGGIFTPTLFVGAAVGYLFGD AQALLP H+SQPFAYAMVGMGAFLAAA
Sbjct: 301 LATSITVGSGAVGGIFTPTLFVGAAVGYLFGDAAQALLPVHISQPFAYAMVGMGAFLAAA 360

Query: 384 SYAPLMAILMIFEMTLSYQVVLPLMLSCVVAYVVARSTDGRSMYEITLKRHRDNEERLRL 443
           SYAPLMAILMIFEMTLSYQVVLPLMLSCVVAYVVARS DGRSMYEITLKRHRDNEERLRL
Sbjct: 361 SYAPLMAILMIFEMTLSYQVVLPLMLSCVVAYVVARSVDGRSMYEITLKRHRDNEERLRL 420

Query: 444 RGTHMSDLIRPADTVLSETASLEEVSALFLKYPVKYVYIVDAHERYQGVVALQDITSWLL 503
           RGTHMS+LIRPA+TVL E ASLEE+SALFLKYPVKYVYIVD  +RY GVVALQDITSWLL
Sbjct: 421 RGTHMSELIRPAETVLHEEASLEEISALFLKYPVKYVYIVDGRQRYLGVVALQDITSWLL 480

Query: 504 DKKEPAGRVARDFLRPHFLHEVTPDMSLGEALQLFLDHQGERLPVIASAEDPRLLGVVFK 563
           DKKE AGRVARDFLRPHFLHEVTPDMSLGEALQLFLDHQGERLPVIASA+DP+LLGVVFK
Sbjct: 481 DKKEAAGRVARDFLRPHFLHEVTPDMSLGEALQLFLDHQGERLPVIASADDPQLLGVVFK 540

Query: 564 TSLLDAYFRLDRAGN 578
           TSLLDAYFRLDRAGN
Sbjct: 541 TSLLDAYFRLDRAGN 555


>ref|YP_004752221.1| putative chloride channel family protein [Collimonas fungivorans
           Ter331]
 gb|AEK61398.1| Putative chloride channel family protein [Collimonas fungivorans
           Ter331]
          Length = 587

 Score =  703 bits (1815), Expect = 0.0,   Method: Composition-based stats.
 Identities = 416/571 (72%), Positives = 478/571 (83%)

Query: 6   IKYRLRVLNACRVSENHSMLLWGALAGFVGALATIAFRECIALLQIWLTGHDGSFVDIAK 65
           ++YR+R+      SE+HSMLLW A+ GF+GALAT+ FRECI  LQ  LTGH GSFV +AK
Sbjct: 12  LRYRIRLQRLFHFSESHSMLLWAAVVGFLGALATVLFRECIYGLQQLLTGHSGSFVAMAK 71

Query: 66  GLSWPVRVLLPTCGGVIAGLFLVWAKRTPAGNGGDYMEAVAIGDGAIPVRNTLLRSISSL 125
            L W +R+LLP  GG++AG  LV AKR P+    DYMEAVAIGDG IPVR +LLRS+SSL
Sbjct: 72  SLPWSMRLLLPAAGGLLAGAILVLAKRVPSSATSDYMEAVAIGDGNIPVRQSLLRSLSSL 131

Query: 126 ASIASGGSIGREGPMVQLSALCASLVGKFSHFPSSRLRLLVACGAAAGITSAYNAPIAGA 185
           A++ SGGSIGREGPMVQL+AL ASLVG+F+HF  +RLRLLVACGAAAGITSAYNAPIAGA
Sbjct: 132 ATVVSGGSIGREGPMVQLAALAASLVGRFTHFSPARLRLLVACGAAAGITSAYNAPIAGA 191

Query: 186 FFITEIVLGSLVMESFGPVVVASVVANITMRELPGYRAAYEMPYFPEIGGWEVLLFLVLG 245
           FF+TEIVLG++VMESFGPVVVASVVANITMRELPGY  AYEMP FP + G E+L F++LG
Sbjct: 192 FFVTEIVLGAIVMESFGPVVVASVVANITMRELPGYHPAYEMPPFPPVAGIEILWFVLLG 251

Query: 246 VLAGILAPQFLRVLELGKQGFGKFKLPLPVRLGVGGLLVGLISIEVPEVWGNGYSLVNSL 305
           ++AGI APQFLR+L   K  F +  LPLP+RLG+GGLLVGLIS+ +PEVWGNGYS+VNSL
Sbjct: 252 IMAGIAAPQFLRLLAFSKHMFTRTALPLPLRLGLGGLLVGLISVWMPEVWGNGYSVVNSL 311

Query: 306 LHTTWLWQAVLMVLVIKVLATAITVGSGAVGGIFTPTLFVGAAVGYLFGDTAQALLPFHM 365
           LHT WLW +VL+VLV+KVLATAIT GSGAVGGIFTPTLFVGAA+GYLFG     +LP   
Sbjct: 312 LHTPWLWWSVLLVLVLKVLATAITTGSGAVGGIFTPTLFVGAAIGYLFGQLLHIVLPDLT 371

Query: 366 SQPFAYAMVGMGAFLAAASYAPLMAILMIFEMTLSYQVVLPLMLSCVVAYVVARSTDGRS 425
           S PFAYAMVGMGAFLAAA+ APLMAILMIFEMTLSYQVVLPLMLSCVVAY +ARS +G S
Sbjct: 372 SAPFAYAMVGMGAFLAAATSAPLMAILMIFEMTLSYQVVLPLMLSCVVAYFIARSINGAS 431

Query: 426 MYEITLKRHRDNEERLRLRGTHMSDLIRPADTVLSETASLEEVSALFLKYPVKYVYIVDA 485
           MY+IT+KR+RD +ER+RLRG HM++LIRPADTVL  TA   E+S LFLKYPVKY+YIVD 
Sbjct: 432 MYDITIKRNRDAQERVRLRGIHMNELIRPADTVLPLTAPFAEISGLFLKYPVKYIYIVDQ 491

Query: 486 HERYQGVVALQDITSWLLDKKEPAGRVARDFLRPHFLHEVTPDMSLGEALQLFLDHQGER 545
             RY GVVALQDITS LL K E  G+VA DF+R  FLH VTP+MSLG+ALQ FLDHQGER
Sbjct: 492 RNRYCGVVALQDITSCLLAKSETQGKVAADFIRREFLHVVTPEMSLGDALQSFLDHQGER 551

Query: 546 LPVIASAEDPRLLGVVFKTSLLDAYFRLDRA 576
           LPV+ S EDP LLG V+KTSLLDAYFRLDR+
Sbjct: 552 LPVVRSHEDPELLGAVYKTSLLDAYFRLDRS 582


>ref|ZP_06842894.1| Cl- channel voltage-gated family protein [Burkholderia sp. Ch1-1]
 gb|EFG69348.1| Cl- channel voltage-gated family protein [Burkholderia sp. Ch1-1]
          Length = 577

 Score =  675 bits (1741), Expect = 0.0,   Method: Composition-based stats.
 Identities = 365/575 (63%), Positives = 448/575 (77%), Gaps = 2/575 (0%)

Query: 1   MHELWIKYRLRVLNACRVSENHSMLLWGALAGFVGALATIAFRECIALLQIWLTGHDGSF 60
           M    +K R R  +  R+SE H+ML+W  + G  GA ATIAFRE IALLQ  + G  GSF
Sbjct: 1   MLSFLLKLRTRAQDLFRLSEAHTMLVWSVVVGVAGAFATIAFREGIALLQFAVVGKAGSF 60

Query: 61  VDIAKGLSWPVRVLLPTCGGVIAGLFLVWAKR-TPAGNGGDYMEAVAIGDGAIPVRNTLL 119
           V++A+ L W VR+ LP  GG+IAG FL+ A+R    GN  DYMEAVAIGDG +PV+ +L 
Sbjct: 61  VEMARSLPWTVRIWLPAAGGLIAGFFLLIARRYEDKGNHVDYMEAVAIGDGVVPVKLSLW 120

Query: 120 RSISSLASIASGGSIGREGPMVQLSALCASLVGKFSHFPSSRLRLLVACGAAAGITSAYN 179
           RS+SSL +I+SGGSIGREGPMVQL+AL ASL+G++ HF  SRLRLLVACGAAAGITSAY+
Sbjct: 121 RSVSSLFTISSGGSIGREGPMVQLAALAASLIGRWVHFDPSRLRLLVACGAAAGITSAYS 180

Query: 180 APIAGAFFITEIVLGSLVMESFGPVVVASVVANITMRELPGYRAAYEMPYFPEIGGWEVL 239
           APIAGAFF+TEIVLGS+ MESFGPVVV++VVANITMRE  GYR  YEMP FP + G EVL
Sbjct: 181 APIAGAFFVTEIVLGSIAMESFGPVVVSAVVANITMREFAGYRPPYEMPVFPPVAGLEVL 240

Query: 240 LFLVLGVLAGILAPQFLRVLELGKQGFGKFKLPLPVRLGVGGLLVGLISIEVPEVWGNGY 299
           LF+ LG+L G  APQFLR+L++ K+ F K  +PLPVRL +GGL+VG++S+  PEVWGNGY
Sbjct: 241 LFVALGLLCGAAAPQFLRLLDVSKKSFRKLPVPLPVRLALGGLVVGILSVRTPEVWGNGY 300

Query: 300 SLVNSLLHTTWLWQAVLMVLVIKVLATAITVGSGAVGGIFTPTLFVGAAVGYLFGDTAQA 359
           S+VNS+LH+ W W A+++VLV K++ATA T GSGAVGG+FTPTLFVGA VG LFG    A
Sbjct: 301 SVVNSILHSPWTWTALVLVLVFKLVATAATSGSGAVGGVFTPTLFVGAVVGSLFGQGMHA 360

Query: 360 LLPFHMSQPFAYAMVGMGAFLAAASYAPLMAILMIFEMTLSYQVVLPLMLSCVVAYVVAR 419
           L P   S PFAYAMVGMGAFLA A+ APLMAILMIFEMTLSYQVVLPLMLSCVVAY V+R
Sbjct: 361 LWPHGTSAPFAYAMVGMGAFLAGATQAPLMAILMIFEMTLSYQVVLPLMLSCVVAYFVSR 420

Query: 420 STDGRSMYEITLKRHRDNEERLRLRGTHMSDLIRPADTVLSETASLEEVSALFLKYPVKY 479
           +    SMYEITL+R+   +ER RLR T M +LIRPA+TV+   A++ +++ +FL+YPVKY
Sbjct: 421 AIGKTSMYEITLRRNHQEQERSRLRATQMRELIRPAETVVPPNATVHDMTRVFLEYPVKY 480

Query: 480 VYIVDAHERYQGVVALQDITSWLLDKKEPAGRVARDFLRPHFLHEVTPDMSLGEALQLFL 539
           +Y+ +    + GVVAL+DITS LLD ++ + + A D+L+PHF   +TPDM LG ALQ FL
Sbjct: 481 LYVANESGAFLGVVALKDITSDLLDGRDTSTKTAADYLQPHF-DVLTPDMPLGVALQHFL 539

Query: 540 DHQGERLPVIASAEDPRLLGVVFKTSLLDAYFRLD 574
             QGERLPV+ SA  P L GVV+KTSLLDAYFR++
Sbjct: 540 AFQGERLPVVESAAHPTLAGVVYKTSLLDAYFRMN 574


>ref|YP_559043.1| putative voltage-gated ClC-type chloride channel ClcB [Burkholderia
           xenovorans LB400]
 gb|ABE30991.1| Putative chloride channel family protein, clcB- like protein
           [Burkholderia xenovorans LB400]
          Length = 591

 Score =  672 bits (1733), Expect = 0.0,   Method: Composition-based stats.
 Identities = 363/570 (63%), Positives = 445/570 (78%), Gaps = 2/570 (0%)

Query: 6   IKYRLRVLNACRVSENHSMLLWGALAGFVGALATIAFRECIALLQIWLTGHDGSFVDIAK 65
           +K R R  +  R+SE H+ML+W  + G  GA ATIAFRE IALLQ  + G  GSFV++A+
Sbjct: 20  LKLRTRAQDLFRLSEAHTMLVWSVVVGVAGAFATIAFREGIALLQTAVVGKAGSFVEMAR 79

Query: 66  GLSWPVRVLLPTCGGVIAGLFLVWAKR-TPAGNGGDYMEAVAIGDGAIPVRNTLLRSISS 124
            L W VR+ LP  GG+IAG FL+ A+R     N  DYMEAVAIGDG +PV+ +  RS+SS
Sbjct: 80  SLPWTVRIWLPAAGGLIAGFFLLIARRHEDKSNHVDYMEAVAIGDGVVPVKLSFWRSVSS 139

Query: 125 LASIASGGSIGREGPMVQLSALCASLVGKFSHFPSSRLRLLVACGAAAGITSAYNAPIAG 184
           L +I+SGGSIGREGPMVQL+AL ASL+G++ HF  +RLRLLVACGAAAGITSAY+APIAG
Sbjct: 140 LFTISSGGSIGREGPMVQLAALAASLIGRWVHFDPARLRLLVACGAAAGITSAYSAPIAG 199

Query: 185 AFFITEIVLGSLVMESFGPVVVASVVANITMRELPGYRAAYEMPYFPEIGGWEVLLFLVL 244
           AFF+TEIVLGS+ MESFGPVVV++VVANITMRE  GYR  YEMP FP + G EVLLF+ L
Sbjct: 200 AFFVTEIVLGSIAMESFGPVVVSAVVANITMREFAGYRPPYEMPVFPPVAGLEVLLFVAL 259

Query: 245 GVLAGILAPQFLRVLELGKQGFGKFKLPLPVRLGVGGLLVGLISIEVPEVWGNGYSLVNS 304
           G+L G  APQFLR+L++ K+ F K  +PLPVRL +GGL+VG++S+  PEVWGNGYS+VNS
Sbjct: 260 GLLCGAAAPQFLRLLDVSKKSFRKLPVPLPVRLALGGLVVGILSVWTPEVWGNGYSVVNS 319

Query: 305 LLHTTWLWQAVLMVLVIKVLATAITVGSGAVGGIFTPTLFVGAAVGYLFGDTAQALLPFH 364
           +LH+ W W A+++VLV K++ATA T GSGAVGG+FTPTLFVGA VG LFG    AL P  
Sbjct: 320 ILHSPWTWTALVLVLVFKLVATAATSGSGAVGGVFTPTLFVGAVVGSLFGQGMHALWPHG 379

Query: 365 MSQPFAYAMVGMGAFLAAASYAPLMAILMIFEMTLSYQVVLPLMLSCVVAYVVARSTDGR 424
            S PFAYAMVGMGAFLA A+ APLMAILMIFEMTLSYQVVLPLMLSCVVAY V+R+    
Sbjct: 380 TSAPFAYAMVGMGAFLAGATQAPLMAILMIFEMTLSYQVVLPLMLSCVVAYFVSRAIGKT 439

Query: 425 SMYEITLKRHRDNEERLRLRGTHMSDLIRPADTVLSETASLEEVSALFLKYPVKYVYIVD 484
           SMYEITL+R+ + +ER RLR T M +LIRPA+TV+   A++ E++ +FL+YPVKY+Y+  
Sbjct: 440 SMYEITLRRNHEEQERSRLRATQMRELIRPAETVVRPNATVHEMTRVFLEYPVKYLYVAS 499

Query: 485 AHERYQGVVALQDITSWLLDKKEPAGRVARDFLRPHFLHEVTPDMSLGEALQLFLDHQGE 544
               + GVVAL+DITS LLD ++ A + A D+L+PHF   +TPDM LG ALQ FL  QGE
Sbjct: 500 ESGAFLGVVALKDITSDLLDGRDTAAKTAADYLQPHF-DVLTPDMPLGVALQHFLAFQGE 558

Query: 545 RLPVIASAEDPRLLGVVFKTSLLDAYFRLD 574
           RLPV+ SA  P L GVV+KTSLLDAYFR++
Sbjct: 559 RLPVVESAAHPTLAGVVYKTSLLDAYFRMN 588


>ref|ZP_02355528.1| putative voltage-gated ClC-type chloride channel ClcB [Burkholderia
           oklahomensis EO147]
          Length = 577

 Score =  657 bits (1694), Expect = 0.0,   Method: Composition-based stats.
 Identities = 360/575 (62%), Positives = 439/575 (76%), Gaps = 2/575 (0%)

Query: 1   MHELWIKYRLRVLNACRVSENHSMLLWGALAGFVGALATIAFRECIALLQIWLTGHDGSF 60
           M    +K R R     R+S+ H+ML+W  + G  GALAT+AFRE I L+Q  ++G  GSF
Sbjct: 1   MLSFLLKLRTRAQTLFRLSDAHTMLVWSVIVGIGGALATVAFREGIELIQRAVSGTSGSF 60

Query: 61  VDIAKGLSWPVRVLLPTCGGVIAGLFLVWAKRTPAGNG-GDYMEAVAIGDGAIPVRNTLL 119
            ++AK L W VR  LP  GG +AG  L+ A+R        DYMEAVA+GDG +PVR +L 
Sbjct: 61  TEMAKRLPWQVRFWLPAAGGFLAGCVLLVAQRGARKESKSDYMEAVALGDGIVPVRQSLW 120

Query: 120 RSISSLASIASGGSIGREGPMVQLSALCASLVGKFSHFPSSRLRLLVACGAAAGITSAYN 179
           RS SSL +I SGGSIGREGPMVQL+AL ASLVG+F+HF   RLRLLVACGAAAGITSAYN
Sbjct: 121 RSASSLLTIGSGGSIGREGPMVQLAALAASLVGRFAHFDPPRLRLLVACGAAAGITSAYN 180

Query: 180 APIAGAFFITEIVLGSLVMESFGPVVVASVVANITMRELPGYRAAYEMPYFPEIGGWEVL 239
           APIAGAFF+ EIVLGS+ MESFGPVVVASVVANI MRE  GY+  YEMP FP + GWEVL
Sbjct: 181 APIAGAFFVCEIVLGSIAMESFGPVVVASVVANIVMREFAGYKPPYEMPVFPAVAGWEVL 240

Query: 240 LFLVLGVLAGILAPQFLRVLELGKQGFGKFKLPLPVRLGVGGLLVGLISIEVPEVWGNGY 299
            F+ LG+L G  APQFLR+L+L K  F +   PLPVRL +GGL+VG+IS+ VP+VWGNGY
Sbjct: 241 FFVALGLLCGAAAPQFLRLLDLSKTQFKRLPAPLPVRLALGGLVVGVISVWVPDVWGNGY 300

Query: 300 SLVNSLLHTTWLWQAVLMVLVIKVLATAITVGSGAVGGIFTPTLFVGAAVGYLFGDTAQA 359
           S+VN++LHT W WQA++ VLV K++ATA T GSGAVGGIFTPTLFVGA VG LFG    A
Sbjct: 301 SVVNAILHTPWTWQALVAVLVFKLIATAATAGSGAVGGIFTPTLFVGAVVGSLFGIALHA 360

Query: 360 LLPFHMSQPFAYAMVGMGAFLAAASYAPLMAILMIFEMTLSYQVVLPLMLSCVVAYVVAR 419
           L+P H+S  +AYAMVGMGAFLA A+ APLMAILMIFEMTLSYQVVLPLM+SCVVAY  AR
Sbjct: 361 LMPAHVSAWYAYAMVGMGAFLAGATQAPLMAILMIFEMTLSYQVVLPLMMSCVVAYFAAR 420

Query: 420 STDGRSMYEITLKRHRDNEERLRLRGTHMSDLIRPADTVLSETASLEEVSALFLKYPVKY 479
           +    SMYEITL+RHRD+E R+RLR T M DLI+PA TV+  TAS+ +++ +FL+YPVKY
Sbjct: 421 AIGKTSMYEITLRRHRDDEARMRLRTTQMRDLIQPAATVVPLTASVADMTRVFLEYPVKY 480

Query: 480 VYIVDAHERYQGVVALQDITSWLLDKKEPAGRVARDFLRPHFLHEVTPDMSLGEALQLFL 539
           +Y+ D   R++G VAL+DITS LL+K++   + A ++L   F   +TPDM +G AL+ F+
Sbjct: 481 LYVTDDAGRFRGAVALKDITSDLLEKRDTTHKRAANYLHTPF-PLLTPDMPIGAALEHFM 539

Query: 540 DHQGERLPVIASAEDPRLLGVVFKTSLLDAYFRLD 574
             QGERLPV+  A+DP L GVV+KTSLLDAY RL+
Sbjct: 540 QFQGERLPVVERADDPTLAGVVYKTSLLDAYRRLN 574