BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.1417.p (620 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003773480.1| periplasmic binding protein [Herbaspirillum ... 645 0.0 ref|YP_004752060.1| small-conductance mechanosensitive ion chann... 489 e-136 ref|YP_558764.1| small-conductance mechanosensitive ion channel ... 348 2e-93 ref|YP_001895577.1| mechanosensitive ion channel MscS [Burkholde... 346 6e-93 ref|ZP_03265867.1| MscS Mechanosensitive ion channel [Burkholder... 345 1e-92 >ref|YP_003773480.1| periplasmic binding protein [Herbaspirillum seropedicae SmR1] gb|ADJ61572.1| periplasmic binding protein [Herbaspirillum seropedicae SmR1] Length = 374 Score = 645 bits (1663), Expect = 0.0, Method: Composition-based stats. Identities = 335/373 (89%), Positives = 355/373 (95%) Query: 246 MNLDLDFLYNMNEGPLRSGLIVLGVALVAVVVGIVLHRMGIALVRRLARGRPFTTTATTV 305 M++DLDFLYNMNEGPLRSGLIVLGVALVAVVV + LHR+GIA+VRRLARGRPFTTTAT V Sbjct: 1 MSIDLDFLYNMNEGPLRSGLIVLGVALVAVVVAVFLHRVGIAVVRRLARGRPFTTTATEV 60 Query: 306 TFNASRTCVILFMLRLVLAGAPDNTPGLTRISYLTSVAFILALTWFLMRCVKSVSTTVVN 365 TF+ASRTCVILFMLRLVL GAPDNTPGLT ISYLTS+ ILALTWFL+RCVKSVS TV+N Sbjct: 61 TFSASRTCVILFMLRLVLTGAPDNTPGLTSISYLTSIGLILALTWFLIRCVKSVSLTVIN 120 Query: 366 LNPYDVADNLKARRILTQTRVLSRSAYFIIALLGLAFVLLTLPGARQFGASLLASAGVAG 425 LNPYDVADNLKARRILTQTRVLSRSAYFIIA+LGL+FVLLTLPGARQFGASLLASAGVAG Sbjct: 121 LNPYDVADNLKARRILTQTRVLSRSAYFIIAMLGLSFVLLTLPGARQFGASLLASAGVAG 180 Query: 426 IVAGIAAKPVLGNFFAGLQIAFSQPIRIDDVLIVKGEWGRVEEITGTFVVVRIWDERRMI 485 IVAGIAAKPVLGNFFAGLQIAFSQPIRIDDVLIVKGEWGRVEEITGTFVVVRIWDERRMI Sbjct: 181 IVAGIAAKPVLGNFFAGLQIAFSQPIRIDDVLIVKGEWGRVEEITGTFVVVRIWDERRMI 240 Query: 486 VPLQWFIENPFENWTHTSSTILGTVFLWLDFAVPTNAIRAEFERVCQTLPLWDKRVCVMH 545 VPLQWFIENPFENWTHTSSTILGTVFLWLDF+VPT AIR EFERVC++LPLWDKRVCVMH Sbjct: 241 VPLQWFIENPFENWTHTSSTILGTVFLWLDFSVPTQAIRDEFERVCKSLPLWDKRVCVMH 300 Query: 546 VTDTSERNMQIRLLVSARDSGSAFDLRCQIREHMIGFIASNYPEALPRLRAEVSSRNRPE 605 VTD SER MQ+RLLVSA+DSGSAFDLRCQIREHMIGFIA NYP ALPRLRAEV +RN PE Sbjct: 301 VTDASERTMQVRLLVSAKDSGSAFDLRCQIREHMIGFIARNYPGALPRLRAEVEARNDPE 360 Query: 606 ALDTIIDGAPGEK 618 A+D+IIDG P +K Sbjct: 361 AIDSIIDGPPTQK 373 >ref|YP_004752060.1| small-conductance mechanosensitive ion channel [Collimonas fungivorans Ter331] gb|AEK61237.1| small-conductance mechanosensitive ion channel [Collimonas fungivorans Ter331] Length = 351 Score = 489 bits (1259), Expect = e-136, Method: Composition-based stats. Identities = 244/319 (76%), Positives = 279/319 (87%) Query: 282 HRMGIALVRRLARGRPFTTTATTVTFNASRTCVILFMLRLVLAGAPDNTPGLTRISYLTS 341 HR+GI L+RRL R RP+ T A+ + + AS+ C++LF +RLVL GAPD+TP L +SYLTS Sbjct: 33 HRVGIMLLRRLVRNRPYITNASFIAYRASQVCLMLFAVRLVLTGAPDDTPWLVPLSYLTS 92 Query: 342 VAFILALTWFLMRCVKSVSTTVVNLNPYDVADNLKARRILTQTRVLSRSAYFIIALLGLA 401 VA I+ALTW MRC++++S TVV LNP ADNLKARRILTQTRVL+RSAYFI ALLGL Sbjct: 93 VALIVALTWLAMRCIRALSATVVQLNPVSAADNLKARRILTQTRVLTRSAYFIAALLGLG 152 Query: 402 FVLLTLPGARQFGASLLASAGVAGIVAGIAAKPVLGNFFAGLQIAFSQPIRIDDVLIVKG 461 FVLLTLPGARQFGASLLASAGVAG+VAGIAA+PVLGNF AG+QIAFSQPIRIDDVLIV+G Sbjct: 153 FVLLTLPGARQFGASLLASAGVAGLVAGIAARPVLGNFIAGMQIAFSQPIRIDDVLIVQG 212 Query: 462 EWGRVEEITGTFVVVRIWDERRMIVPLQWFIENPFENWTHTSSTILGTVFLWLDFAVPTN 521 EWGRVEEITGTFVVVRIWDERR+IVPLQWFIENPFENWTHTSSTILGTVFLWLDF VP Sbjct: 213 EWGRVEEITGTFVVVRIWDERRLIVPLQWFIENPFENWTHTSSTILGTVFLWLDFGVPVP 272 Query: 522 AIRAEFERVCQTLPLWDKRVCVMHVTDTSERNMQIRLLVSARDSGSAFDLRCQIREHMIG 581 A+RAEFER+C+ LWD RVCV+HVTD +ER MQ+RLLVSARDSG+AFDLRC IRE M+ Sbjct: 273 AVRAEFERICKQSSLWDGRVCVLHVTDANERAMQLRLLVSARDSGTAFDLRCAIREAMLA 332 Query: 582 FIASNYPEALPRLRAEVSS 600 FIA NYP++LPRLRA + + Sbjct: 333 FIAGNYPDSLPRLRAALDA 351 >ref|YP_558764.1| small-conductance mechanosensitive ion channel [Burkholderia xenovorans LB400] gb|ABE30712.1| small-conductance mechanosensitive ion channel [Burkholderia xenovorans LB400] Length = 407 Score = 348 bits (892), Expect = 2e-93, Method: Composition-based stats. Identities = 177/351 (50%), Positives = 245/351 (69%) Query: 250 LDFLYNMNEGPLRSGLIVLGVALVAVVVGIVLHRMGIALVRRLARGRPFTTTATTVTFNA 309 LD L + E PL + L L V+++AV++ + +HR+G + R AR P T+ Sbjct: 4 LDDLSRIAEAPLHTWLGALIVSVIAVLLAVGIHRIGARMAARFARPYPLTSVVLRYIDKP 63 Query: 310 SRTCVILFMLRLVLAGAPDNTPGLTRISYLTSVAFILALTWFLMRCVKSVSTTVVNLNPY 369 S +++ L V APD +T + ++ I A+TW +R ++ ++ +P Sbjct: 64 SLFVLVILALEAVWWEAPDTLTFITPLRDAAAIGLIGAITWLSVRSAAAIGEAIIQAHPL 123 Query: 370 DVADNLKARRILTQTRVLSRSAYFIIALLGLAFVLLTLPGARQFGASLLASAGVAGIVAG 429 D ADNL+ARRI TQ RVL+RS F+I ++G+ L+T P RQ GASLLASAGVAG+VAG Sbjct: 124 DTADNLQARRIHTQARVLARSVMFVIVIVGVGGALMTFPNVRQIGASLLASAGVAGLVAG 183 Query: 430 IAAKPVLGNFFAGLQIAFSQPIRIDDVLIVKGEWGRVEEITGTFVVVRIWDERRMIVPLQ 489 IAA+PVLGN AGLQIA SQPIR+DDV++++GEWGR+EEITGT+V VR+WD+RR+IVPLQ Sbjct: 184 IAARPVLGNLIAGLQIALSQPIRLDDVVVIQGEWGRIEEITGTYVSVRLWDQRRLIVPLQ 243 Query: 490 WFIENPFENWTHTSSTILGTVFLWLDFAVPTNAIRAEFERVCQTLPLWDKRVCVMHVTDT 549 WFIENPF NWT +SS I+GTVFL++D+ +P +R E R+ + P WD+RV V+ VTD Sbjct: 244 WFIENPFSNWTRSSSQIIGTVFLYVDYRMPLAPLREELARIVEDAPEWDRRVQVLQVTDG 303 Query: 550 SERNMQIRLLVSARDSGSAFDLRCQIREHMIGFIASNYPEALPRLRAEVSS 600 +ER+MQ+R LVS+ DSG +DLRC++RE ++ FI +YP+ LPR RAEVS+ Sbjct: 304 TERSMQLRALVSSLDSGLNWDLRCRVREGLLDFIQKHYPQYLPRARAEVSA 354 >ref|YP_001895577.1| mechanosensitive ion channel MscS [Burkholderia phytofirmans PsJN] gb|ACD16353.1| MscS Mechanosensitive ion channel [Burkholderia phytofirmans PsJN] Length = 407 Score = 346 bits (888), Expect = 6e-93, Method: Composition-based stats. Identities = 176/351 (50%), Positives = 245/351 (69%) Query: 250 LDFLYNMNEGPLRSGLIVLGVALVAVVVGIVLHRMGIALVRRLARGRPFTTTATTVTFNA 309 LD L M E PL + L V+++A+++ + +H++G +V RLAR P T+ Sbjct: 4 LDDLSRMAEAPLHTWPGALIVSVIAMMLAVGIHQIGARIVTRLARPYPLTSVVLRYIDKP 63 Query: 310 SRTCVILFMLRLVLAGAPDNTPGLTRISYLTSVAFILALTWFLMRCVKSVSTTVVNLNPY 369 S +++ L V APD +T + ++ I +TW +R ++ ++ +P Sbjct: 64 SLFVLVILALEAVWWEAPDTLTFITPLRDAAAITLIGGITWLSVRSAAAIGEAIIQAHPL 123 Query: 370 DVADNLKARRILTQTRVLSRSAYFIIALLGLAFVLLTLPGARQFGASLLASAGVAGIVAG 429 D ADNL+ARRI TQ RVL+RS F+I ++G+ L+T P RQ GASLLASAGVAG+VAG Sbjct: 124 DTADNLQARRIHTQARVLARSVMFVIVIVGVGGALMTFPSVRQIGASLLASAGVAGLVAG 183 Query: 430 IAAKPVLGNFFAGLQIAFSQPIRIDDVLIVKGEWGRVEEITGTFVVVRIWDERRMIVPLQ 489 IAA+PVLGN AGLQIA SQPIR+DDV++++GEWGR+EEITGT+V VR+WD+RR+IVPLQ Sbjct: 184 IAARPVLGNLIAGLQIALSQPIRLDDVVVIQGEWGRIEEITGTYVSVRLWDQRRLIVPLQ 243 Query: 490 WFIENPFENWTHTSSTILGTVFLWLDFAVPTNAIRAEFERVCQTLPLWDKRVCVMHVTDT 549 WFIENPF NWT +SS I+GTVFL++D+ +P +R E R+ + P WD+RV V+ VTD Sbjct: 244 WFIENPFSNWTRSSSQIIGTVFLYVDYRMPLAPLREELARIVEDAPEWDRRVQVLQVTDG 303 Query: 550 SERNMQIRLLVSARDSGSAFDLRCQIREHMIGFIASNYPEALPRLRAEVSS 600 +ER+MQ+R LVS+ DSG +DLRC++RE ++ FI +YP+ LPR RAEVS+ Sbjct: 304 TERSMQLRALVSSLDSGLNWDLRCRVREGLLDFIQQHYPQYLPRARAEVSA 354 >ref|ZP_03265867.1| MscS Mechanosensitive ion channel [Burkholderia sp. H160] gb|EEA02558.1| MscS Mechanosensitive ion channel [Burkholderia sp. H160] Length = 407 Score = 345 bits (885), Expect = 1e-92, Method: Composition-based stats. Identities = 179/351 (50%), Positives = 239/351 (68%) Query: 250 LDFLYNMNEGPLRSGLIVLGVALVAVVVGIVLHRMGIALVRRLARGRPFTTTATTVTFNA 309 +D Y + PL + L V L+ +V I +HR G +V RLA+ P T+ Sbjct: 4 IDDFYRVAAAPLHTWFGALIVTLIVTIVAIGIHRFGARIVMRLAQPHPLTSVVLRYIDKP 63 Query: 310 SRTCVILFMLRLVLAGAPDNTPGLTRISYLTSVAFILALTWFLMRCVKSVSTTVVNLNPY 369 S + + + V + APD + + +T VA I ALTW +R + ++ +P Sbjct: 64 SLFALEILVFAWVWSEAPDTLNFIEPLRDVTGVALIAALTWLSVRLAAGIGEAIIRAHPL 123 Query: 370 DVADNLKARRILTQTRVLSRSAYFIIALLGLAFVLLTLPGARQFGASLLASAGVAGIVAG 429 D ADNL+ARRI TQ RVL+RS +I +G+ L+TLP RQ GASLLASAGVAG+VAG Sbjct: 124 DTADNLQARRIHTQARVLARSVMIVIVFIGVGGALMTLPNVRQIGASLLASAGVAGLVAG 183 Query: 430 IAAKPVLGNFFAGLQIAFSQPIRIDDVLIVKGEWGRVEEITGTFVVVRIWDERRMIVPLQ 489 IAA+PVLGN AGLQIA SQPIR+DDV++++ EWGRVEEITGT+V VR+WD+RR+IVPLQ Sbjct: 184 IAARPVLGNLIAGLQIALSQPIRLDDVVVIQNEWGRVEEITGTYVSVRLWDQRRLIVPLQ 243 Query: 490 WFIENPFENWTHTSSTILGTVFLWLDFAVPTNAIRAEFERVCQTLPLWDKRVCVMHVTDT 549 WFIENPF NWT +SS I+G+VFL++D+ +P +R E R+ + P WD+RV V+ VTD Sbjct: 244 WFIENPFLNWTRSSSQIIGSVFLFVDYRMPLAPLREELARIVENAPEWDRRVQVLQVTDC 303 Query: 550 SERNMQIRLLVSARDSGSAFDLRCQIREHMIGFIASNYPEALPRLRAEVSS 600 +ER MQ+R LVSA DSG A+DLRC++RE ++ F+ +YPE LPR RAEVS+ Sbjct: 304 TERAMQLRALVSAPDSGLAWDLRCRVREGLLDFVHRHYPEYLPRARAEVST 354