BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.1172.p (484 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003774796.1| major facilitator superfamily permease [Herb... 466 e-129 ref|YP_004152850.1| major facilitator superfamily protein [Vario... 333 5e-89 ref|YP_439474.1| major facilitator superfamily protein [Burkhold... 332 6e-89 ref|ZP_02493729.1| transporter, major facilitator family protein... 325 9e-87 ref|YP_004350468.1| major facilitator superfamily protein superf... 325 9e-87 >ref|YP_003774796.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] gb|ADJ62888.1| permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 485 Score = 466 bits (1198), Expect = e-129, Method: Composition-based stats. Identities = 362/481 (75%), Positives = 401/481 (83%), Gaps = 1/481 (0%) Query: 1 MTTSSSAPPAQRQAQPQPASVASWGELLGRRHGWCAVALTGGVALYAINVHIVTTILPSV 60 MTTSSSAPPAQRQAQPQPASVASWGELLGRRHGWCAVALTGGVA++AINVHIVTTILPSV Sbjct: 1 MTTSSSAPPAQRQAQPQPASVASWGELLGRRHGWCAVALTGGVAMHAINVHIVTTILPSV 60 Query: 61 VRDIGGLAYYAWNVTLFVAASIVGSALTGKLMGAMGARATYLMALALFGSGALLCGSAGS 120 VRDIGGL YYAWNVTLFV SI+GS LTGKL+ +GARA YL+ALA+FG+GAL+C AGS Sbjct: 61 VRDIGGLDYYAWNVTLFVVTSIIGSTLTGKLLARLGARAAYLLALAVFGAGALVCAGAGS 120 Query: 121 MGWMLAGRAVQGIGGGLLAALAYALIPLVFEPRMWPRAIALVSGMWGVSTLLGPAVGGLF 180 MGWMLAGRAVQG+GGGLLA+L YALIPLVFE R+W RAIALVSGMWGVSTLLGPAVGGLF Sbjct: 121 MGWMLAGRAVQGVGGGLLASLGYALIPLVFEQRLWSRAIALVSGMWGVSTLLGPAVGGLF 180 Query: 181 AASGHWRVAFFALLPLLAAQAWLVWRQLGPATVRSASGPAQAVPLGRIALLASSVLLVAL 240 AA GHWR+AFFALLPLL Q+ LV RQLGP VR+ S QA+PLGRI LLA SVLLVAL Sbjct: 181 AAGGHWRLAFFALLPLLLLQSVLVVRQLGPGRVRAQSDAVQAMPLGRIFLLALSVLLVAL 240 Query: 241 AAQADDDWLASACVLAGVLLAVWMARIDLRHRANLLPHAAYRPRTVMGGVMACIALLVMG 300 AAQ W A ACVL G L W+ARIDLRHR +LLP YR TVMG V ACI+LLVM Sbjct: 241 AAQTGQAWSALACVLGGAGLGAWVARIDLRHRISLLPRDGYRLSTVMGCVFACISLLVMA 300 Query: 301 SCVEIFLPYFLQGLHGYDPLGAGYATAAMAAGWSLGSLGGSGLSGRAALRMIRLGPCMMT 360 SCVEIF+PYFLQ LHGY PL AGYA AAMA GWS+GSL GSG S +AA RMIR+GP +M Sbjct: 301 SCVEIFVPYFLQSLHGYAPLAAGYAAAAMAGGWSVGSLVGSGRSAQAAARMIRMGPWLML 360 Query: 361 LGMALLAFLMPAQWLPAGALADGLIVLALGVTGLGIGMGWPHLVARALHAAQPGEENLTS 420 LG+ LA L+PA L AG +AD LIVLAL GLGIG+GWPHLVARA+ AA+PGEENLTS Sbjct: 361 LGLLALAALLPASLLAAGVVADVLIVLALAAAGLGIGVGWPHLVARAMQAARPGEENLTS 420 Query: 421 SAITTVQLFAMALGAALAGLVANQAGLG-AGGAEATARAAWVLLALFTLAPALAIFVARS 479 SAITTVQL+AMA+GAALAGL+ANQAGLG GG + +AA VL A+F +APALAI + R Sbjct: 421 SAITTVQLYAMAMGAALAGLIANQAGLGMPGGVDGARQAAGVLFAVFAVAPALAIMLTRR 480 Query: 480 V 480 + Sbjct: 481 L 481 >ref|YP_004152850.1| major facilitator superfamily protein [Variovorax paradoxus EPS] gb|ADU34739.1| major facilitator superfamily MFS_1 [Variovorax paradoxus EPS] Length = 487 Score = 333 bits (854), Expect = 5e-89, Method: Composition-based stats. Identities = 248/463 (53%), Positives = 315/463 (68%), Gaps = 7/463 (1%) Query: 8 PPAQRQAQPQPASV-----ASWGELLGRRHGWCAVALTGGVALYAINVHIVTTILPSVVR 62 PPA + P+S+ A+WGEL R+GW A+ALTGGVAL+A+NVHIVTT+LPSVVR Sbjct: 2 PPAASSSVADPSSISPPATAAWGELFTGRNGWRALALTGGVALHAVNVHIVTTVLPSVVR 61 Query: 63 DIGGLAYYAWNVTLFVAASIVGSALTGKLMGAMGARATYLMALALFGSGALLCGSAGSMG 122 +IGGL +YAW+ TLFV SI+G+ L+ +L+ +G R L ALA+F +G++ C A +M Sbjct: 62 EIGGLDWYAWSTTLFVVGSILGATLSVRLLAVLGPRGACLAALAVFTAGSVGCALAPAMH 121 Query: 123 WMLAGRAVQGIGGGLLAALAYALIPLVFEPRMWPRAIALVSGMWGVSTLLGPAVGGLFAA 182 W+LAGR VQG+GGG+LAAL+Y LI LVF R+WPRA+ALVSGMWGV+TL GPAVGGLFA Sbjct: 122 WLLAGRTVQGLGGGMLAALSYGLIQLVFAQRLWPRAVALVSGMWGVATLCGPAVGGLFAQ 181 Query: 183 SGHWRVAFFALLPLLAAQAWLVWRQLGPATVRSASGPAQA--VPLGRIALLASSVLLVAL 240 SGHWR AF+ LLP+ AAQA LV QL P + PA+ +P +IALLA SVL++A Sbjct: 182 SGHWRWAFWFLLPVAAAQALLVTVQLRPGAGVHHARPAKMPRIPALQIALLAISVLVIAA 241 Query: 241 AAQADDDWLASACVLAGVLLAVWMARIDLRHRANLLPHAAYRPRTVMGGVMACIALLVMG 300 + D L +A V+ G+ + V R+D LLP AY +G + A +ALL++G Sbjct: 242 SGLLADLALQAAGVVLGLGVGVVATRVDREATVRLLPTGAYMLSAPLGAIYASVALLLIG 301 Query: 301 SCVEIFLPYFLQGLHGYDPLGAGYATAAMAAGWSLGSLGGSGLSGRAALRMIRLGPCMMT 360 + EIF+PYFLQ LHG+ PL AGY TAAMA GWS GSL SG SG A RM+R GP T Sbjct: 302 TTTEIFVPYFLQLLHGHSPLAAGYLTAAMAGGWSAGSLLSSGRSGAGADRMLRAGPVACT 361 Query: 361 LGMALLAFLMPAQWLPAGALADGLIVLALGVTGLGIGMGWPHLVARALHAAQPGEENLTS 420 LG+ LA L+P L+ +AL V G G+G+GWPHLV R L A GEE L S Sbjct: 362 LGLVALALLLPFPGHLGAGFETALVAMALAVVGAGVGIGWPHLVTRVLSLAPKGEEGLAS 421 Query: 421 SAITTVQLFAMALGAALAGLVANQAGLGAGGAEATARAAWVLL 463 ++ITT+QL+ MA+GAA+AGLVAN AGL G A++A V L Sbjct: 422 ASITTIQLYGMAVGAAVAGLVANAAGLTLPGGVEGAQSAAVWL 464 >ref|YP_439474.1| major facilitator superfamily protein [Burkholderia thailandensis E264] gb|ABC33954.1| major facilitator superfamily protein superfamily [Burkholderia thailandensis E264] Length = 467 Score = 332 bits (852), Expect = 6e-89, Method: Composition-based stats. Identities = 237/446 (53%), Positives = 311/446 (69%), Gaps = 6/446 (1%) Query: 20 SVASWGELLGRRHGWCAVALTGGVALYAINVHIVTTILPSVVRDIGGLAYYAWNVTLFVA 79 + SWGELL R+G ++AL GGVAL+AIN++I TTILPSVVRDIGGL YYAWN TLF+A Sbjct: 5 TAPSWGELLSGRNGLRSIALAGGVALHAINIYIATTILPSVVRDIGGLEYYAWNTTLFMA 64 Query: 80 ASIVGSALTGKLMGAMGARATYLMALALFGSGALLCGSAGSMGWMLAGRAVQGIGGGLLA 139 ASIVG+ L+ ++ G RA YL+AL +F +G L C A M WML GRA QG GGG+L Sbjct: 65 ASIVGAPLSANVLSRFGPRAAYLVALVVFCAGTLACALARDMPWMLVGRAAQGFGGGILF 124 Query: 140 ALAYALIPLVFEPRMWPRAIALVSGMWGVSTLLGPAVGGLFAASGHWRVAFFALLPLLAA 199 AL+YALI +VF+ R+W RA+A+VSGMWGV+TL GPA+GG+FA SG WR+AF AL+P+ A Sbjct: 125 ALSYALIRIVFDERLWSRAMAMVSGMWGVATLCGPAIGGVFAQSGTWRLAFVALVPVAAV 184 Query: 200 QAWLVWRQLGPATVRSASGPAQAVP-LGRIALLASSVLLVALAAQADDDWLASACVLAGV 258 A +V QL R ASG A P +G+I LLA+SVL+V++A+ + + A V AG+ Sbjct: 185 LALIVIVQL---PAREASGARAARPAIGKILLLAASVLVVSVASLSKEIVANVAGVAAGL 241 Query: 259 LLAVWMARIDLRHRANLLPHAAYRPRTVMGGVMACIALLVMGSCVEIFLPYFLQGLHGYD 318 +A+ +AR + LLP AY R +G + AC++LLV+G EIF+PYFLQ +HGY Sbjct: 242 AIALLIARFERGAARRLLPTGAYDVRAPLGAIYACMSLLVIGMTTEIFVPYFLQIIHGYP 301 Query: 319 PLGAGYATAAMAAGWSLGSLGGSGLSGRAALRMIRLGPCMMTLGMALLAFLMPAQWLPAG 378 PL AGY TA MAAGW++GSL SG SG AA ++R GP ++ + + LA ++P Q L AG Sbjct: 302 PLLAGYLTALMAAGWTVGSLFSSGRSGAAARALVRGGPLVVVIALVALALVVPPQHLLAG 361 Query: 379 ALADGLIVLALGVTGLGIGMGWPHLVARALHAAQPGEENLTSSAITTVQLFAMALGAALA 438 + AL G+GIG+GWPHL+ + L A G+E+L S++ITTVQL+A A+G+ALA Sbjct: 362 GPGLAALCAALAAVGVGIGVGWPHLLTQVLTNAPAGQEDLASTSITTVQLYATAIGSALA 421 Query: 439 GLVANQAGLGAGGAEATAR--AAWVL 462 GLVAN AG A G A A+ AAW+ Sbjct: 422 GLVANLAGFSAPGGLAGAQHAAAWLF 447 >ref|ZP_02493729.1| transporter, major facilitator family protein [Burkholderia pseudomallei NCTC 13177] Length = 467 Score = 325 bits (834), Expect = 9e-87, Method: Composition-based stats. Identities = 247/463 (53%), Positives = 319/463 (68%), Gaps = 5/463 (1%) Query: 20 SVASWGELLGRRHGWCAVALTGGVALYAINVHIVTTILPSVVRDIGGLAYYAWNVTLFVA 79 + SWGELL R+G ++AL GGVAL+AIN++I TTILPSVVRDIGGL YYAWN TLF+A Sbjct: 5 TAPSWGELLSGRNGLRSIALAGGVALHAINIYIATTILPSVVRDIGGLEYYAWNTTLFMA 64 Query: 80 ASIVGSALTGKLMGAMGARATYLMALALFGSGALLCGSAGSMGWMLAGRAVQGIGGGLLA 139 ASIVG+ L+ ++ G RA YL+AL +F +G L C A M WML GRA QG GGG+L Sbjct: 65 ASIVGAPLSANVLSRFGPRAAYLVALVVFCAGTLACAGAKDMPWMLVGRAAQGFGGGILF 124 Query: 140 ALAYALIPLVFEPRMWPRAIALVSGMWGVSTLLGPAVGGLFAASGHWRVAFFALLPLLAA 199 AL+YALI +VF+ R+W RA+A+VSGMWGV+TL GPA+GG+FA SG WR+AF AL+P+ A Sbjct: 125 ALSYALIRIVFDERLWSRAMAMVSGMWGVATLCGPAIGGVFAQSGTWRLAFVALVPVAAV 184 Query: 200 QAWLVWRQLGPATVRSASGPAQAVP-LGRIALLASSVLLVALAAQADDDWLASACVLAGV 258 A +V QL R ASG A P +G+I LLA SVL+V++A+ + A V AG+ Sbjct: 185 LALIVIVQL---PAREASGARAARPAIGKILLLAVSVLVVSVASLSKAIVANVAGVAAGL 241 Query: 259 LLAVWMARIDLRHRANLLPHAAYRPRTVMGGVMACIALLVMGSCVEIFLPYFLQGLHGYD 318 +A+ +AR++ LLP AY R +G + AC++LLV+G EIF+PYFLQ +HGY Sbjct: 242 AIALLIARLERGATRRLLPTGAYDVRAPLGAIYACMSLLVIGMTTEIFVPYFLQIIHGYP 301 Query: 319 PLGAGYATAAMAAGWSLGSLGGSGLSGRAALRMIRLGPCMMTLGMALLAFLMPAQWLPAG 378 PL AGY TA MAAGW+ GSL SG SG AA ++R GP ++ + + LA ++P Q L AG Sbjct: 302 PLLAGYLTALMAAGWTAGSLLSSGRSGAAAQALVRGGPLVVVIALVALALVVPPQHLLAG 361 Query: 379 ALADGLIVLALGVTGLGIGMGWPHLVARALHAAQPGEENLTSSAITTVQLFAMALGAALA 438 + AL G+GIG+GWPHL+ + L A G+E+L S++ITTVQL+A A+G+ALA Sbjct: 362 GAGLAALCAALAAVGVGIGVGWPHLLTQVLTNAPAGQEDLASTSITTVQLYATAIGSALA 421 Query: 439 GLVANQAGLGAGGAEATAR-AAWVLLALFTLAPALAIFVARSV 480 GLVAN AG A G A A+ AA L A+F AP LA VAR V Sbjct: 422 GLVANLAGFSAPGGLAGAQHAAAWLFAVFAAAPVLAAIVARRV 464 >ref|YP_004350468.1| major facilitator superfamily protein superfamily [Burkholderia gladioli BSR3] gb|AEA64956.1| major facilitator superfamily protein superfamily [Burkholderia gladioli BSR3] Length = 484 Score = 325 bits (834), Expect = 9e-87, Method: Composition-based stats. Identities = 240/463 (51%), Positives = 314/463 (67%), Gaps = 3/463 (0%) Query: 20 SVASWGELLGRRHGWCAVALTGGVALYAINVHIVTTILPSVVRDIGGLAYYAWNVTLFVA 79 + +SWGELL R+G ++AL GGVAL+AIN+++ TTILPSVV +IGGL YYAWN TLF+A Sbjct: 6 TTSSWGELLSGRNGLRSIALAGGVALHAINIYLATTILPSVVHEIGGLEYYAWNTTLFMA 65 Query: 80 ASIVGSALTGKLMGAMGARATYLMALALFGSGALLCGSAGSMGWMLAGRAVQGIGGGLLA 139 ASIVG+ L+ L+ +G RA YL+ALA+F +G L C +A M WMLAGR QG GGG+L Sbjct: 66 ASIVGAPLSTNLLARVGPRAAYLVALAVFCAGTLACAAARDMPWMLAGRVAQGFGGGILV 125 Query: 140 ALAYALIPLVFEPRMWPRAIALVSGMWGVSTLLGPAVGGLFAASGHWRVAFFALLPLLAA 199 AL+YALI +VF PR+W R + +VSGMWG++TL GPA+GGLFA SGHWR+AF ALLP+ A Sbjct: 126 ALSYALIRIVFAPRLWSRGMGMVSGMWGIATLCGPAIGGLFAESGHWRLAFLALLPIAAL 185 Query: 200 QAWLVWRQLGPATVRSASGPAQAVPLGRIALLASSVLLVALAAQADDDWLASACVLAGVL 259 A +V QL + A PA LGRI LLA+SVL+V++A+ + A + GV Sbjct: 186 LALIVIAQLPARQTQRA--PAAPAALGRILLLAASVLVVSVASLSRSAAANVAGIAVGVA 243 Query: 260 LAVWMARIDLRHRANLLPHAAYRPRTVMGGVMACIALLVMGSCVEIFLPYFLQGLHGYDP 319 L +W+AR++ R LLP AY R +G + AC++LL +G EIF+PYFLQ +H P Sbjct: 244 LTLWIARLERDPRVRLLPSGAYSLRQPLGAIYACMSLLGIGVTTEIFVPYFLQTIHAQPP 303 Query: 320 LGAGYATAAMAAGWSLGSLGGSGLSGRAALRMIRLGPCMMTLGMALLAFLMPAQWLPAGA 379 L AGY +A MAAGWS+GSL GSG RAA ++R GP ++ L + LA LMP L A Sbjct: 304 LLAGYLSALMAAGWSIGSLVGSGRGPRAARVLMRGGPVLVALALLALALLMPVPGLLAAG 363 Query: 380 LADGLIVLALGVTGLGIGMGWPHLVARALHAAQPGEENLTSSAITTVQLFAMALGAALAG 439 + AL GLG+GMGWPHL+ + L A G+E+L S+++TTVQL+A A+G+ALAG Sbjct: 364 AGMAALCGALVAVGLGVGMGWPHLLTQVLTRAPAGQEDLASASLTTVQLYATAIGSALAG 423 Query: 440 LVANQAGLG-AGGAEATARAAWVLLALFTLAPALAIFVARSVT 481 LVAN G GG + +AA L A F +AP LA + R V+ Sbjct: 424 LVANLGGFTEPGGLDGARQAAMWLFAAFAIAPLLAAILVRRVS 466