BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.1126.p (435 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_585850.1| protocatechuate 4,5-dioxygenase [Cupriavidus me... 667 0.0 emb|CCA82162.1| protocatechuate 4,5-dioxygenase (ligAB fusion pr... 653 0.0 ref|YP_003750357.1| protocatechuate 4,5-dioxygenase (ligab fusio... 653 0.0 emb|CCA86983.1| protocatechuate 4,5-dioxygenase (ligAB fusion pr... 652 0.0 ref|ZP_00944630.1| Hypothetical protein RRSL_02092 [Ralstonia so... 652 0.0 >ref|YP_585850.1| protocatechuate 4,5-dioxygenase [Cupriavidus metallidurans CH34] gb|ABF10581.1| protocatechuate 4,5-dioxygenase (ligAB fusion protein) [Cupriavidus metallidurans CH34] Length = 430 Score = 667 bits (1722), Expect = 0.0, Method: Composition-based stats. Identities = 317/430 (73%), Positives = 357/430 (83%), Gaps = 3/430 (0%) Query: 1 MANIVGAIATSHTPTIGFALDANKQHDAVWAPIFKAYEPVQQWLQEKQPDVLLVVYNDHV 60 MA I+GAIA+SHTPTIGFALDA KQ D WAPIF AY PV +WL K+PDVLLVVYNDHV Sbjct: 1 MATIIGAIASSHTPTIGFALDAGKQQDPAWAPIFAAYAPVSEWLARKKPDVLLVVYNDHV 60 Query: 61 TSFFFDHYSAFALGVGSEYQVADEGGGARKLPPVGGHAALARHIGSSLMADEFDMAFFQN 120 TSFFFDHYSAFALG+G Y ADEGGG R LPP GH LARH+G+SL+ADEFDM+FFQ+ Sbjct: 61 TSFFFDHYSAFALGIGDTYHAADEGGGPRNLPPARGHVELARHVGASLVADEFDMSFFQD 120 Query: 121 KPLDHGCFSPLSMLWPHEKEWPGQIIPLQVGVLQFPIPTARRCYKLGQGLRRAIESYPED 180 KPLDHGCFSP+S++ PHE EWP +I+PLQVGVLQFP P+ARRCYKLGQ +RRA+ESYPED Sbjct: 121 KPLDHGCFSPMSVMLPHEGEWPVKILPLQVGVLQFPAPSARRCYKLGQAIRRAVESYPED 180 Query: 181 LDVVIVATGGLSHQVHGERAGFNNTPWDHQFLDLLQHDPEALTRLTHAELATLGGLESAE 240 L V IVATGGLSHQVHGERAGFNNT WDHQFLDL + DPE+L +THAE A GG ESAE Sbjct: 181 LKVAIVATGGLSHQVHGERAGFNNTAWDHQFLDLFESDPESLLDITHAEYAKRGGFESAE 240 Query: 241 VIMWLIMRGAMPAGVNCRHREYYLPSMTGIAVAVYEPAVPVDAALQSAEVARHREHVAQQ 300 V+MW++MRGAM + V C HR+YYLPSMTGIAVAVYE AL+ A R REH+ Q Sbjct: 241 VVMWMVMRGAMSSRVRCLHRDYYLPSMTGIAVAVYENESADAPALREARAERQREHIGHQ 300 Query: 301 LKGIERLQGTYPFTLETANARYRINRYLYQLIQPAFRERFREDPQATYEEAGLSQEECDM 360 L GIE+L GTYPFTLET+ YRINRYL+QLI PAFRERFR DP ATY+EA L+ EE DM Sbjct: 301 LHGIEQLDGTYPFTLETSVRAYRINRYLHQLIDPAFRERFRADPLATYDEAALTPEERDM 360 Query: 361 ISRRDWQAMIRYGVIFFLLEKLGAVVGVSNLHIYAAMRGQSLEEFQQTRNAPGALYSVAG 420 I+RRDW+ MI YGVIFF+LEKLGAV GVSNLHIYAAMRGQSLE+FQ+TRNAPGALYSVAG Sbjct: 361 ITRRDWRGMIHYGVIFFMLEKLGAVCGVSNLHIYAAMRGQSLEDFQKTRNAPGALYSVAG 420 Query: 421 AASDKAADWN 430 + S A W+ Sbjct: 421 SGS---ASWS 427 >emb|CCA82162.1| protocatechuate 4,5-dioxygenase (ligAB fusion protein) [blood disease bacterium R229] Length = 441 Score = 653 bits (1685), Expect = 0.0, Method: Composition-based stats. Identities = 317/434 (73%), Positives = 361/434 (83%), Gaps = 1/434 (0%) Query: 1 MANIVGAIATSHTPTIGFALDANKQHDAVWAPIFKAYEPVQQWLQEKQPDVLLVVYNDHV 60 MA IVGAIA+SHTPTIGFALDA KQ D VWAPIF AY PV WL +PDVLLV+YNDHV Sbjct: 1 MATIVGAIASSHTPTIGFALDAAKQQDPVWAPIFAAYAPVSDWLARARPDVLLVIYNDHV 60 Query: 61 TSFFFDHYSAFALGVGSEYQVADEGGGARKLPPVGGHAALARHIGSSLMADEFDMAFFQN 120 TSFFFDHYSAFALG+G Y ADEGGGAR+LPP+ GHA LARH+G+SL+ADEFDMAFFQ+ Sbjct: 61 TSFFFDHYSAFALGIGQSYAPADEGGGARQLPPIAGHAGLARHVGASLVADEFDMAFFQD 120 Query: 121 KPLDHGCFSPLSMLWPHEKEWPGQIIPLQVGVLQFPIPTARRCYKLGQGLRRAIESYPED 180 KPLDHGCFSPLS++ PH+ WP +I+PLQVGVLQFP P+ARRCYKLGQ +RRA+ESYPED Sbjct: 121 KPLDHGCFSPLSVMLPHQDGWPVKILPLQVGVLQFPAPSARRCYKLGQAIRRAVESYPED 180 Query: 181 LDVVIVATGGLSHQVHGERAGFNNTPWDHQFLDLLQHDPEALTRLTHAELATLGGLESAE 240 L V IVATGGLSHQVHGERAGFNNT WD FLDL + DPE L LTHA+ A LGG ESAE Sbjct: 181 LKVAIVATGGLSHQVHGERAGFNNTAWDQTFLDLFEADPEPLLELTHADYARLGGFESAE 240 Query: 241 VIMWLIMRGAMPAGVNCRHREYYLPSMTGIAVAVYEPAVPVDAALQSAEVARHREHVAQQ 300 V+MW++MRGAM + + C HR+YYLPSMTGIAVAVYE DA +Q+ R RE + Q Sbjct: 241 VVMWMVMRGAMASRIRCLHRDYYLPSMTGIAVAVYENEAAPDAGMQAERARRQRERIGHQ 300 Query: 301 LKGIERLQGTYPFTLETANARYRINRYLYQLIQPAFRERFREDPQATYEEAGLSQEECDM 360 L+GIE L+GTYPFTLET+ YRINRYL+QLIQPAFR RF DP ATY+EAGLS +E D+ Sbjct: 301 LRGIEALEGTYPFTLETSVRAYRINRYLHQLIQPAFRARFLADPVATYDEAGLSAQERDL 360 Query: 361 ISRRDWQAMIRYGVIFFLLEKLGAVVGVSNLHIYAAMRGQSLEEFQQTRNAPGALYSVAG 420 I+RRDW+ +I YGVIFFLLEKLGAV+GVSNLHIYAAMRGQSL +FQ+TRNAPGALYSV+G Sbjct: 361 ITRRDWRGLIHYGVIFFLLEKLGAVIGVSNLHIYAAMRGQSLADFQKTRNAPGALYSVSG 420 Query: 421 AASDKAADWNKNGT 434 AA DK A W+ T Sbjct: 421 AA-DKGAAWSGAST 433 >ref|YP_003750357.1| protocatechuate 4,5-dioxygenase (ligab fusion protein) [Ralstonia solanacearum PSI07] emb|CBJ35737.1| protocatechuate 4,5-dioxygenase (ligAB fusion protein) [Ralstonia solanacearum PSI07] Length = 441 Score = 653 bits (1685), Expect = 0.0, Method: Composition-based stats. Identities = 317/434 (73%), Positives = 361/434 (83%), Gaps = 1/434 (0%) Query: 1 MANIVGAIATSHTPTIGFALDANKQHDAVWAPIFKAYEPVQQWLQEKQPDVLLVVYNDHV 60 MA IVGAIA+SHTPTIGFALDA KQ D VWAPIF AY PV WL +PDVLLV+YNDHV Sbjct: 1 MATIVGAIASSHTPTIGFALDAAKQQDPVWAPIFAAYAPVSDWLARARPDVLLVIYNDHV 60 Query: 61 TSFFFDHYSAFALGVGSEYQVADEGGGARKLPPVGGHAALARHIGSSLMADEFDMAFFQN 120 TSFFFDHYSAFALG+G Y ADEGGGAR+LPP+ GHA LARH+G+SL+ADEFDMAFFQ+ Sbjct: 61 TSFFFDHYSAFALGIGQSYAPADEGGGARQLPPIAGHAGLARHVGASLVADEFDMAFFQD 120 Query: 121 KPLDHGCFSPLSMLWPHEKEWPGQIIPLQVGVLQFPIPTARRCYKLGQGLRRAIESYPED 180 KPLDHGCFSPLS++ PH+ WP +I+PLQVGVLQFP P+ARRCYKLGQ +RRA+ESYPED Sbjct: 121 KPLDHGCFSPLSVMLPHQDGWPVKILPLQVGVLQFPAPSARRCYKLGQAIRRAVESYPED 180 Query: 181 LDVVIVATGGLSHQVHGERAGFNNTPWDHQFLDLLQHDPEALTRLTHAELATLGGLESAE 240 L V IVATGGLSHQVHGERAGFNNT WD FLDL + DPE L LTHA+ A LGG ESAE Sbjct: 181 LKVAIVATGGLSHQVHGERAGFNNTAWDQTFLDLFEADPEPLLELTHADYARLGGFESAE 240 Query: 241 VIMWLIMRGAMPAGVNCRHREYYLPSMTGIAVAVYEPAVPVDAALQSAEVARHREHVAQQ 300 V+MW++MRGAM + + C HR+YYLPSMTGIAVAVYE DA +Q+ R RE + Q Sbjct: 241 VVMWMVMRGAMASRIRCLHRDYYLPSMTGIAVAVYENEAAPDAGMQAERARRQRERIGHQ 300 Query: 301 LKGIERLQGTYPFTLETANARYRINRYLYQLIQPAFRERFREDPQATYEEAGLSQEECDM 360 L+GIE L+GTYPFTLET+ YRINRYL+QLIQPAFR RF DP ATY+EAGLS +E D+ Sbjct: 301 LRGIEALEGTYPFTLETSVRAYRINRYLHQLIQPAFRARFLADPVATYDEAGLSAQERDL 360 Query: 361 ISRRDWQAMIRYGVIFFLLEKLGAVVGVSNLHIYAAMRGQSLEEFQQTRNAPGALYSVAG 420 I+RRDW+ +I YGVIFFLLEKLGAV+GVSNLHIYAAMRGQSL +FQ+TRNAPGALYSV+G Sbjct: 361 ITRRDWRGLIHYGVIFFLLEKLGAVIGVSNLHIYAAMRGQSLADFQKTRNAPGALYSVSG 420 Query: 421 AASDKAADWNKNGT 434 AA DK A W+ T Sbjct: 421 AA-DKGAAWSGAST 433 >emb|CCA86983.1| protocatechuate 4,5-dioxygenase (ligAB fusion protein) [Ralstonia syzygii R24] Length = 441 Score = 652 bits (1683), Expect = 0.0, Method: Composition-based stats. Identities = 316/434 (72%), Positives = 361/434 (83%), Gaps = 1/434 (0%) Query: 1 MANIVGAIATSHTPTIGFALDANKQHDAVWAPIFKAYEPVQQWLQEKQPDVLLVVYNDHV 60 MA IVGAIA+SHTPTIGFALDA KQ D VWAPIF AY PV WL +PDVLLV+YNDHV Sbjct: 1 MATIVGAIASSHTPTIGFALDAAKQQDPVWAPIFAAYAPVSDWLARARPDVLLVIYNDHV 60 Query: 61 TSFFFDHYSAFALGVGSEYQVADEGGGARKLPPVGGHAALARHIGSSLMADEFDMAFFQN 120 TSFFFDHYSAFALG+G Y ADEGGGAR+LPP+ GHA LARH+G+SL+ADEFDMAFFQ+ Sbjct: 61 TSFFFDHYSAFALGIGQSYAPADEGGGARQLPPIAGHAGLARHVGASLVADEFDMAFFQD 120 Query: 121 KPLDHGCFSPLSMLWPHEKEWPGQIIPLQVGVLQFPIPTARRCYKLGQGLRRAIESYPED 180 KPLDHGCFSPLS++ PH+ WP +I+PLQVGVLQFP P+ARRCYKLGQ +RRA+ESYPED Sbjct: 121 KPLDHGCFSPLSVMLPHQDGWPVKILPLQVGVLQFPAPSARRCYKLGQAIRRAVESYPED 180 Query: 181 LDVVIVATGGLSHQVHGERAGFNNTPWDHQFLDLLQHDPEALTRLTHAELATLGGLESAE 240 L V +VATGGLSHQVHGERAGFNNT WD FLDL + DPE L LTHA+ A LGG ESAE Sbjct: 181 LKVALVATGGLSHQVHGERAGFNNTAWDQTFLDLFEADPEPLLELTHADYARLGGFESAE 240 Query: 241 VIMWLIMRGAMPAGVNCRHREYYLPSMTGIAVAVYEPAVPVDAALQSAEVARHREHVAQQ 300 V+MW++MRGAM + + C HR+YYLPSMTGIAVAVYE DA +Q+ R RE + Q Sbjct: 241 VVMWMVMRGAMASRIRCLHRDYYLPSMTGIAVAVYENEAAPDAGMQAERARRQRERIGHQ 300 Query: 301 LKGIERLQGTYPFTLETANARYRINRYLYQLIQPAFRERFREDPQATYEEAGLSQEECDM 360 L+GIE L+GTYPFTLET+ YRINRYL+QLIQPAFR RF DP ATY+EAGLS +E D+ Sbjct: 301 LRGIEALEGTYPFTLETSVRAYRINRYLHQLIQPAFRARFLADPVATYDEAGLSAQERDL 360 Query: 361 ISRRDWQAMIRYGVIFFLLEKLGAVVGVSNLHIYAAMRGQSLEEFQQTRNAPGALYSVAG 420 I+RRDW+ +I YGVIFFLLEKLGAV+GVSNLHIYAAMRGQSL +FQ+TRNAPGALYSV+G Sbjct: 361 ITRRDWRGLIHYGVIFFLLEKLGAVIGVSNLHIYAAMRGQSLADFQKTRNAPGALYSVSG 420 Query: 421 AASDKAADWNKNGT 434 AA DK A W+ T Sbjct: 421 AA-DKGAAWSGAST 433 >ref|ZP_00944630.1| Hypothetical protein RRSL_02092 [Ralstonia solanacearum UW551] ref|YP_002257029.1| protocatechuate 4,5-dioxygenase beta chain subunit b protein [Ralstonia solanacearum IPO1609] gb|EAP72914.1| Hypothetical protein RRSL_02092 [Ralstonia solanacearum UW551] emb|CAQ58910.1| protocatechuate 4,5-dioxygenase beta chain subunit b protein [Ralstonia solanacearum IPO1609] Length = 446 Score = 652 bits (1681), Expect = 0.0, Method: Composition-based stats. Identities = 320/430 (74%), Positives = 361/430 (83%), Gaps = 1/430 (0%) Query: 1 MANIVGAIATSHTPTIGFALDANKQHDAVWAPIFKAYEPVQQWLQEKQPDVLLVVYNDHV 60 MA IVGAIA+SHTPTIGFALDA KQ D VWAPIF AY PV WL + +PDVLLV+YNDHV Sbjct: 1 MATIVGAIASSHTPTIGFALDAEKQQDPVWAPIFAAYAPVSAWLAQARPDVLLVIYNDHV 60 Query: 61 TSFFFDHYSAFALGVGSEYQVADEGGGARKLPPVGGHAALARHIGSSLMADEFDMAFFQN 120 TSFFFDHYSAFALG+G Y ADEGGGAR LPP+ GH LARH+G+SL+ADEFDMAFFQ+ Sbjct: 61 TSFFFDHYSAFALGIGESYAPADEGGGARPLPPIAGHVGLARHVGASLVADEFDMAFFQD 120 Query: 121 KPLDHGCFSPLSMLWPHEKEWPGQIIPLQVGVLQFPIPTARRCYKLGQGLRRAIESYPED 180 KPLDHGCFSPLS++ PH+ WP +I+PLQVGVLQFP P+ARRCYKLGQ +RRA+ESYPED Sbjct: 121 KPLDHGCFSPLSVMLPHQDGWPVKILPLQVGVLQFPAPSARRCYKLGQAIRRAVESYPED 180 Query: 181 LDVVIVATGGLSHQVHGERAGFNNTPWDHQFLDLLQHDPEALTRLTHAELATLGGLESAE 240 L V IVATGGLSHQVHGERAGFNNT WD FLDL + DPE L LTHA+ A LGG ESAE Sbjct: 181 LRVAIVATGGLSHQVHGERAGFNNTAWDKAFLDLFESDPEQLLELTHADYARLGGFESAE 240 Query: 241 VIMWLIMRGAMPAGVNCRHREYYLPSMTGIAVAVYEPAVPVDAALQSAEVARHREHVAQQ 300 V+MW++MRGAM + + C HR+YYLPSMTGIAVAVYE P DAALQ+A R REH+ Q Sbjct: 241 VVMWMVMRGAMASCIRCLHRDYYLPSMTGIAVAVYENDTPADAALQAARAQRQREHIGHQ 300 Query: 301 LKGIERLQGTYPFTLETANARYRINRYLYQLIQPAFRERFREDPQATYEEAGLSQEECDM 360 L+GIE L+GTYPFTLET+ YRINRYL+QLI PAFR RF DP ATY+EAGLS +E D+ Sbjct: 301 LRGIEALEGTYPFTLETSVRAYRINRYLHQLIGPAFRARFLADPAATYDEAGLSAQERDL 360 Query: 361 ISRRDWQAMIRYGVIFFLLEKLGAVVGVSNLHIYAAMRGQSLEEFQQTRNAPGALYSVAG 420 I+RRDW+ +I YGVIFFLLEKLGAVVGVSNLHIYAAMRGQSL EFQ+TRNAPGALYSV+G Sbjct: 361 ITRRDWRGLIHYGVIFFLLEKLGAVVGVSNLHIYAAMRGQSLAEFQKTRNAPGALYSVSG 420 Query: 421 AASDKAADWN 430 AA K A W+ Sbjct: 421 AAG-KGAAWS 429