BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.0809.p (446 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_05135936.1| diguanylate cyclase/phosphodiesterase [Stenot... 253 4e-65 ref|YP_004314624.1| EAL domain protein [Marinomonas mediterranea... 201 1e-49 ref|YP_050186.1| putative signaling membrane protein [Pectobacte... 200 5e-49 gb|AAS20372.1| unknown [Pectobacterium atrosepticum] 199 6e-49 ref|ZP_06685421.1| EAL-containing signal transduction protein [A... 195 2e-47 >ref|ZP_05135936.1| diguanylate cyclase/phosphodiesterase [Stenotrophomonas sp. SKA14] gb|EED39997.1| diguanylate cyclase/phosphodiesterase [Stenotrophomonas sp. SKA14] Length = 484 Score = 253 bits (647), Expect = 4e-65, Method: Composition-based stats. Identities = 157/439 (35%), Positives = 246/439 (56%), Gaps = 30/439 (6%) Query: 2 CSEGDLTILRNITYANSYVADLGRIEQGVVRCTALWGKLDKLTRLPEPHYHARGGFSVWS 61 CS+ D+ L+ I + +SY++D+GRI++G + C+ALWG++ +LP PHY + G +W+ Sbjct: 42 CSDEDVRALKEIAFNSSYMSDVGRIQEGHLLCSALWGRIVPF-KLPAPHY-SSGETKLWN 99 Query: 62 HQEVLQTHYQ-SNLVALNRSLAIASPSTFDLNRFYQDVSLRITNGNGTHTYAQRDGVRYH 120 +++ + Y +NL+A ++ ++SPS+FD + + + + ++ + + Sbjct: 100 TSDLVGSPYGGTNLIAQGQTFTVSSPSSFDGLDPARTSTFTVETRDRSYVFRSVE----- 154 Query: 121 PAGTTDLLPS--IDIKECRRAMNLCAQASAHLHWYALAHPLAFLALLASSLL---IGALA 175 PA S I + C N+CA S P + LA SLL IG A Sbjct: 155 PATDASEAASSRIRLSRCSNKANVCAFVS---------RPRRGIWDLAPSLLYTIIGIGA 205 Query: 176 CL----LYLRLRRNQGVVEKLRE----GIANDEIVLLYQPIVCVRDLSLKGYEALARWRP 227 L YL +RRN+ + L++ + +EI L+YQP+ V D L G+E L+RW P Sbjct: 206 ALGITFSYLLIRRNRTARQTLQQRFSLAMRRNEIELVYQPLYTVVDRRLVGFEVLSRWSP 265 Query: 228 ADEPEIGPDIFVPLAAQNRLSTALAKVVLARALREMQDLLQADPMLVLAVNTEVADIEDA 287 A E EI P IFVP+A Q LS+ L + VL++ALREM L + L++N E D+ Sbjct: 266 AGEGEISPGIFVPMARQLGLSSDLFRYVLSKALREMAPALAGENAPYLSINAEPVDMASE 325 Query: 288 DFIEFALELTAATPGLRPQLHLEVTERADIRSATFTQNIELLRQAGFAIWIDDFGTGSAN 347 D + F + +T L Q+ +E+TER ++ T ++ L GF IDDFGTGS+N Sbjct: 326 DTVRFVVSVTQQAGVLPDQVRIEITEREELVCPGVTAHMAALAALGFRFLIDDFGTGSSN 385 Query: 348 LSHLSYAQFDGIKIDRLFTSALSTGSPLRSVVPNLLRLATELELKVVIEGIETEEQLQLI 407 SH + + F GIKIDR+F SA++ SPLR V+P + +A +L L+V++EG+ET EQ L+ Sbjct: 386 FSHFAQSPFIGIKIDRMFVSAITEESPLRPVLPGMYLIARQLGLEVIVEGVETAEQDALL 445 Query: 408 RSIAPQIHAQGWLFSKAME 426 + IAP QGWL+ ++ Sbjct: 446 QQIAPSAVGQGWLYGTPVQ 464 >ref|YP_004314624.1| EAL domain protein [Marinomonas mediterranea MMB-1] gb|ADZ92788.1| EAL domain protein [Marinomonas mediterranea MMB-1] Length = 542 Score = 201 bits (512), Expect = 1e-49, Method: Composition-based stats. Identities = 129/445 (28%), Positives = 232/445 (52%), Gaps = 20/445 (4%) Query: 1 MCSEGDLTILRNITYANSYVADLGRIEQGVVRCTALWGKLDKLTRLPEPHYHARGGFSVW 60 +CS+ D+T LR +++ Y+ D+GRI V CTA WGKL +LP P G W Sbjct: 84 LCSDDDITFLREYLWSHKYIKDVGRIVNDDVACTAGWGKLTTPVQLPTPTKSWPSGSKHW 143 Query: 61 SH-QEVLQTHYQSNLVALNRSLAIASPSTFDLNRFYQ-DVSLRITNGNGTHTYAQRDGVR 118 + ++ + + + NL+A ++ + +P++++ N L I +G + + + Sbjct: 144 RNVNDMFRDNVRVNLLAYKNTIVVLAPTSYEQNLLKPPHTDLAIATADGQYVFQVVGDL- 202 Query: 119 YHPAGTTDLLPSID--------IKECRRAMNLCAQASAHLH--WYALAHPLAFLALLASS 168 + +L+ + I C + ++C A W L FL+++A Sbjct: 203 ----SSNELMEKVKSTNFHQLAIHTCLKDNSICVWAFKKKSGLWAQSDFFLGFLSVIAV- 257 Query: 169 LLIGALACLLYLRLRRNQGVVEKLREGIANDEIVLLYQPIVCVRDLSLKGYEALARWRPA 228 L+ G L LL + +L+ + + + +++QP V ++D + G EAL RW+ Sbjct: 258 LISGGLFQLLNTIYNTRNSMQSRLKRALLHKSLFVVFQPKVNLQDERITGIEALVRWQDE 317 Query: 229 DEPEIGPDIFVPLAAQNRLSTALAKVVLARALREMQDLLQADPMLVLAVNTEVADIEDAD 288 + PD FV +A Q +L L+++V+ ++L+E +LL+ D L L++N +AD+ D Sbjct: 318 HFGTVPPDEFVTVAEQYQLMPLLSRLVIEKSLQEAGELLKNDSSLHLSINLSMADLSDES 377 Query: 289 FIEFALELTAATPGL-RPQLHLEVTERADIRSATFTQNIELLRQAGFAIWIDDFGTGSAN 347 +EF ++L + G+ + +L E+TER+ + Q+++ G+ I +DDFGTG AN Sbjct: 378 LLEF-IDLQRSQQGIAKHRLIFEITERSAVGFDQIEQSVDRFVSHGYRISLDDFGTGYAN 436 Query: 348 LSHLSYAQFDGIKIDRLFTSALSTGSPLRSVVPNLLRLATELELKVVIEGIETEEQLQLI 407 LS LS + IK+D++FT ++ + ++++ + +L LEL+ V EGIE++EQ I Sbjct: 437 LSWLSKIKAAEIKVDKIFTQSIGKNTTNQNMLSAIFKLVENLELQTVFEGIESKEQADYI 496 Query: 408 RSIAPQIHAQGWLFSKAMELREVLQ 432 S QGWLF+K M++ E+ Q Sbjct: 497 VSQNKNAIGQGWLFAKPMKIEELQQ 521 >ref|YP_050186.1| putative signaling membrane protein [Pectobacterium atrosepticum SCRI1043] emb|CAG74993.1| putative signaling membrane protein [Pectobacterium atrosepticum SCRI1043] Length = 539 Score = 200 bits (508), Expect = 5e-49, Method: Composition-based stats. Identities = 137/438 (31%), Positives = 219/438 (50%), Gaps = 14/438 (3%) Query: 2 CSEGDLTILRNITYANSYVADLGRIEQGVVRCTALWGKLDKLTRLPEPHYHARGGFSVWS 61 CS DL LR I++ + Y+ D+GRI+ + C+A WG L+ L P+ G +W+ Sbjct: 87 CSSADLKELRLISFYSVYLRDIGRIKNNYLICSAGWGMLNPPIYLLPPNLTTPDGVQLWT 146 Query: 62 -HQEVLQTHYQSNLVALNRSLAIASPSTFDLNRFYQ---DVSLRITNGNGTHTYAQRDGV 117 ++V+ +++ +LN + I + + F RF Q + S + N H Y + + Sbjct: 147 AMKDVVDPRITADMASLNGVVTITAAAAF--RRFTQPPAEHSAMLLTRNLDHVYQKFGDI 204 Query: 118 RYHPAGTTDLLPSIDIKECRRAMNLCAQAS-----AHLHWYA-LAHPLAFLA-LLASSLL 170 T + + +R CAQ A L L HP +A LL LL Sbjct: 205 DLPAFRQTRQQNNAWLTMGKRQTFFCAQNRDICVLAQLDSAGILYHPWYVIASLLFLGLL 264 Query: 171 IGALACLLY-LRLRRNQGVVEKLREGIANDEIVLLYQPIVCVRDLSLKGYEALARWRPAD 229 IGA + Y L R+Q + +L+ I + + YQP++ + ++ G EALARWR Sbjct: 265 IGASSTFSYQLYYDRHQALPSQLKRAIKHQRFQVHYQPLISLPQRAIIGVEALARWRNEH 324 Query: 230 EPEIGPDIFVPLAAQNRLSTALAKVVLARALREMQDLLQADPMLVLAVNTEVADIEDADF 289 + P+ F+ +A + L +++ +AL +MQ L +P +L++N V DI D+ Sbjct: 325 GDNVSPEYFINIAEEMGYLPELTRIITRQALHDMQPYLTQEPPFLLSINLSVTDIVSPDY 384 Query: 290 IEFALELTAATPGLRPQLHLEVTERADIRSATFTQNIELLRQAGFAIWIDDFGTGSANLS 349 F ++ R ++ LE+TER+ T +E LR+ G+ + +DDFGTG +NL Sbjct: 385 HHFLQKMCDELGIDRARIMLELTERSSTSHQTLANGLEALRREGYKVALDDFGTGYSNLD 444 Query: 350 HLSYAQFDGIKIDRLFTSALSTGSPLRSVVPNLLRLATELELKVVIEGIETEEQLQLIRS 409 +LS+ FD IKID++F A+ T S ++ L L +L++ V+IEG+ET EQ I Sbjct: 445 YLSHLPFDMIKIDKVFVGAIGTDSVNAAMADLLFTLVKKLDVPVIIEGVETREQAAYILQ 504 Query: 410 IAPQIHAQGWLFSKAMEL 427 P QGW FSKA+ L Sbjct: 505 QCPSAMIQGWYFSKAVAL 522 >gb|AAS20372.1| unknown [Pectobacterium atrosepticum] Length = 526 Score = 199 bits (507), Expect = 6e-49, Method: Composition-based stats. Identities = 137/438 (31%), Positives = 219/438 (50%), Gaps = 14/438 (3%) Query: 2 CSEGDLTILRNITYANSYVADLGRIEQGVVRCTALWGKLDKLTRLPEPHYHARGGFSVWS 61 CS DL LR I++ + Y+ D+GRI+ + C+A WG L+ L P+ G +W+ Sbjct: 74 CSSADLKELRLISFYSVYLRDIGRIKNNYLICSAGWGMLNPPIYLLPPNLTTPDGVQLWT 133 Query: 62 -HQEVLQTHYQSNLVALNRSLAIASPSTFDLNRFYQ---DVSLRITNGNGTHTYAQRDGV 117 ++V+ +++ +LN + I + + F RF Q + S + N H Y + + Sbjct: 134 AMKDVVDPRITADMASLNGVVTITAAAAF--RRFTQPPAEHSAMLLTRNLDHVYQKFGDI 191 Query: 118 RYHPAGTTDLLPSIDIKECRRAMNLCAQAS-----AHLHWYA-LAHPLAFLA-LLASSLL 170 T + + +R CAQ A L L HP +A LL LL Sbjct: 192 DLPAFRQTRQQNNAWLTMGKRQTFFCAQNRDICVLAQLDSAGILYHPWYVIASLLFLGLL 251 Query: 171 IGALACLLY-LRLRRNQGVVEKLREGIANDEIVLLYQPIVCVRDLSLKGYEALARWRPAD 229 IGA + Y L R+Q + +L+ I + + YQP++ + ++ G EALARWR Sbjct: 252 IGASSTFSYQLYYDRHQALPSQLKRAIKHQRFQVHYQPLISLPQRAIIGVEALARWRNEH 311 Query: 230 EPEIGPDIFVPLAAQNRLSTALAKVVLARALREMQDLLQADPMLVLAVNTEVADIEDADF 289 + P+ F+ +A + L +++ +AL +MQ L +P +L++N V DI D+ Sbjct: 312 GDNVSPEYFINIAEEMGYLPELTRIITRQALHDMQPYLTQEPPFLLSINLSVTDIVSPDY 371 Query: 290 IEFALELTAATPGLRPQLHLEVTERADIRSATFTQNIELLRQAGFAIWIDDFGTGSANLS 349 F ++ R ++ LE+TER+ T +E LR+ G+ + +DDFGTG +NL Sbjct: 372 HHFLQKMCDELGIDRARIMLELTERSSTSHQTLANGLEALRREGYKVALDDFGTGYSNLD 431 Query: 350 HLSYAQFDGIKIDRLFTSALSTGSPLRSVVPNLLRLATELELKVVIEGIETEEQLQLIRS 409 +LS+ FD IKID++F A+ T S ++ L L +L++ V+IEG+ET EQ I Sbjct: 432 YLSHLPFDMIKIDKVFVGAIGTDSVNAAMADLLFTLVKKLDVPVIIEGVETREQAAYILQ 491 Query: 410 IAPQIHAQGWLFSKAMEL 427 P QGW FSKA+ L Sbjct: 492 QCPSAMIQGWYFSKAVAL 509 >ref|ZP_06685421.1| EAL-containing signal transduction protein [Achromobacter piechaudii ATCC 43553] gb|EFF77646.1| EAL-containing signal transduction protein [Achromobacter piechaudii ATCC 43553] Length = 497 Score = 195 bits (495), Expect = 2e-47, Method: Composition-based stats. Identities = 134/444 (30%), Positives = 220/444 (49%), Gaps = 19/444 (4%) Query: 1 MCSEGDLTILRNITYANSYVADLGRIEQGVVRCTALWGKLDKLTRLPEPHYHARGGFSVW 60 +CS D+ LR + + +++ D+GR+ G++ C+A WG L LP + R G +W Sbjct: 56 LCSPSDVATLRVLLFNATFLRDVGRLRDGMLVCSAAWGVLQTPHALPPAAFATRNGVRLW 115 Query: 61 -SHQEVLQTHYQSNLVALNRSLAIASPSTFD-LNRFYQDVSLRITNGNGTHTY---AQRD 115 ++ ++ A N + +P FD + ++ +G H Y + D Sbjct: 116 VGAANIMDRRIIGDMAAQNNVVVFTAPGAFDGFPEPGAGIDAKLITRDGRHVYRRFGRID 175 Query: 116 GVRYH----PAGTTDLLPSIDIKECRR---AMNLCAQASAHLH-WYALAHPLAFLALLAS 167 G+ PA + L I EC + ++CA + H W+ L A + Sbjct: 176 GLDLQDGQAPANYS-LRAERVIAECADEPGSPDICAVSRTFAHGWFGLP---ALILACTG 231 Query: 168 SLLIGALACLLYLRLRRNQGVVEKLREGIANDEIVLLYQPIVCVRDLSLKGYEALARWRP 227 +L G LA L L ++++ LR I ++ + YQP+ + L G EALARWR Sbjct: 232 ALTGGLLAGLCVLWRQQSKSDRSALRRAIRRGDLQVCYQPLRELATRRLVGVEALARWRT 291 Query: 228 ADEPEIGPDIFVPLAAQNRLSTALAKVVLARALREMQDLLQADPMLVLAVNTEVADIEDA 287 D ++ P FVP+A L L++ V +ALRE+ L+AD +++N D++D Sbjct: 292 CDGQDVPPASFVPMAEDIGLGRELSRRVTEKALRELSARLRADTEFYVSINVSAQDLQDD 351 Query: 288 DFIEFALELTAATPGLRPQ-LHLEVTERADIRSATFTQNIELLRQAGFAIWIDDFGTGSA 346 + +F L + G+ P+ + LE+TE + A I LR+ GF I IDDFGTG++ Sbjct: 352 AYSDFLLRMVN-DQGIAPRRVALEITEGTPLSDAPVLSTIRALRRQGFRILIDDFGTGNS 410 Query: 347 NLSHLSYAQFDGIKIDRLFTSALSTGSPLRSVVPNLLRLATELELKVVIEGIETEEQLQL 406 NL++L+ Q D IK+DR FT A+ T ++ +++ ++ EL L +++EGIETE Q Q Sbjct: 411 NLNYLAEIQADAIKLDRRFTQAIGTDQAGTLIIDHVVDISRELGLGLIVEGIETEAQAQY 470 Query: 407 IRSIAPQIHAQGWLFSKAMELREV 430 + P QGWL +A+ +V Sbjct: 471 LAQRQPATIGQGWLLGRAVPAEDV 494