BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.0751.p (431 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003774066.1| poly-beta-hydroxyalkanoate depolymerase [Her... 721 0.0 ref|YP_004754476.1| polyhydroxyalkanoate depolymerase [Collimona... 606 e-171 ref|YP_001242104.1| hypothetical protein BBta_6279 [Bradyrhizobi... 502 e-140 ref|ZP_06841365.1| polyhydroxyalkanoate depolymerase, intracellu... 499 e-139 ref|YP_552404.1| polyhydroxyalkanoate depolymerase [Burkholderia... 495 e-138 >ref|YP_003774066.1| poly-beta-hydroxyalkanoate depolymerase [Herbaspirillum seropedicae SmR1] gb|ADJ62158.1| poly-beta-hydroxyalkanoate depolymerase protein [Herbaspirillum seropedicae SmR1] Length = 408 Score = 721 bits (1861), Expect = 0.0, Method: Composition-based stats. Identities = 359/408 (87%), Positives = 382/408 (93%) Query: 24 MINTYQLYQNYADATDPLRACARMMAPLLGATWPGVPVNPALRKMASACEVFARTQLTHA 83 MI TYQLYQNYADATDPLRACARMMA LGATWPG+PV+P RKMASACEVFARTQLTHA Sbjct: 1 MIPTYQLYQNYADATDPLRACARMMAQALGATWPGIPVHPYWRKMASACEVFARTQLTHA 60 Query: 84 RPPFEIGEVLDGGRTIAVHEEEVSATPFCGLLHFRKESSAVQPKVLVVAPMSGHFATLLR 143 RPPF I V + G TI+VHEEE+ ATPFCGLLHFRK+S+ VQPKVLVVAPMSGHFATLLR Sbjct: 61 RPPFGIATVEEEGCTISVHEEEIHATPFCGLLHFRKDSATVQPKVLVVAPMSGHFATLLR 120 Query: 144 GTVKTLLRDHDVYITDWRNARDVAVEHGRFGLDEYVSHVIDFLDMLGPGAHLLAVCQPTV 203 GTV+TLLRDHDVYITDWRNARDVA HGRFGLDEYVSH+IDFL +LGPGAHLLAVCQPTV Sbjct: 121 GTVRTLLRDHDVYITDWRNARDVATAHGRFGLDEYVSHIIDFLGVLGPGAHLLAVCQPTV 180 Query: 204 AALTAASVMAEDNHPAQPRTLTLMAGPLDTRVNPTAVNALAKSKPISWFEKNMISTVPAR 263 AALTAA+VMA D HPAQPR++TLMAGP+DTRVNPTAVNALAKSKPI+WFEKNMISTVPAR Sbjct: 181 AALTAAAVMAADGHPAQPRSMTLMAGPIDTRVNPTAVNALAKSKPIAWFEKNMISTVPAR 240 Query: 264 HGGAGRRVYPGFVQLSAFMNMNLNRHVDAFRKLYHHLVEGEEAQAAQIKDFYEEYFAMAD 323 H GAGRRVYPGFVQL+AFMNMNL+RHV+AF KLYHHLV+GE A+A QIKDFYEEYFAMAD Sbjct: 241 HAGAGRRVYPGFVQLAAFMNMNLSRHVEAFGKLYHHLVDGEHARADQIKDFYEEYFAMAD 300 Query: 324 LPAEFYLETVRTVFQEHALPLGKLTYRERVVEPRAIRRTALFTIEGEKDDICAVGQTVAA 383 LPAEFYLETVRTVFQEHALPLGKL+Y R VEPRAIRRTALFTIEGEKDDICAVGQT+AA Sbjct: 301 LPAEFYLETVRTVFQEHALPLGKLSYAGRPVEPRAIRRTALFTIEGEKDDICAVGQTLAA 360 Query: 384 QEMCSNIRPYMRLHHVQTAVGHYGVFNGRRWDNEIYPRLRDFINMHHR 431 QE+CS IRPYMRLHHVQTAVGHYGVFNGRRWDNEIYPRLRDFINMHHR Sbjct: 361 QELCSGIRPYMRLHHVQTAVGHYGVFNGRRWDNEIYPRLRDFINMHHR 408 >ref|YP_004754476.1| polyhydroxyalkanoate depolymerase [Collimonas fungivorans Ter331] gb|AEK63653.1| Polyhydroxyalkanoate depolymerase [Collimonas fungivorans Ter331] Length = 499 Score = 606 bits (1563), Expect = e-171, Method: Composition-based stats. Identities = 284/416 (68%), Positives = 341/416 (81%) Query: 15 KRDNRGRRFMINTYQLYQNYADATDPLRACARMMAPLLGATWPGVPVNPALRKMASACEV 74 + N+ M YQ YQ YAD +DP+RA A AP+LG +PG P + ALRK+A+ACE+ Sbjct: 84 RHSNKQGSPMTKIYQAYQGYADFSDPMRAWAGAAAPMLGYRFPGWPDSLALRKLAAACEL 143 Query: 75 FARTQLTHARPPFEIGEVLDGGRTIAVHEEEVSATPFCGLLHFRKESSAVQPKVLVVAPM 134 F+ TQLTH RP F I V + GRT++VHEE TPFC LL FRKE+ AVQP++L+VAPM Sbjct: 144 FSCTQLTHQRPAFGIDSVEENGRTVSVHEEISDQTPFCTLLRFRKETKAVQPRILLVAPM 203 Query: 135 SGHFATLLRGTVKTLLRDHDVYITDWRNARDVAVEHGRFGLDEYVSHVIDFLDMLGPGAH 194 SGHFATLLRGTV TLLRDHDVYITDW NARDVA+ GRFG DEYV+H+I F++ +GPG H Sbjct: 204 SGHFATLLRGTVNTLLRDHDVYITDWHNARDVALSDGRFGFDEYVAHLIRFIEKIGPGTH 263 Query: 195 LLAVCQPTVAALTAASVMAEDNHPAQPRTLTLMAGPLDTRVNPTAVNALAKSKPISWFEK 254 ++AVCQPTVAAL A S+MA D HPAQP ++TLMAGP+DTR+NPTAVN LAKSKPI WF+K Sbjct: 264 VVAVCQPTVAALVAVSLMAADKHPAQPSSMTLMAGPIDTRINPTAVNQLAKSKPIEWFQK 323 Query: 255 NMISTVPARHGGAGRRVYPGFVQLSAFMNMNLNRHVDAFRKLYHHLVEGEEAQAAQIKDF 314 N++STVP R G+ R+VYPGF+QL+AFM+MNL RH+ AF+ LY++LV+GE+ +A IK F Sbjct: 324 NLVSTVPGRFAGSQRQVYPGFLQLNAFMSMNLERHLKAFQNLYNYLVDGEDEKANTIKTF 383 Query: 315 YEEYFAMADLPAEFYLETVRTVFQEHALPLGKLTYRERVVEPRAIRRTALFTIEGEKDDI 374 Y EYFAM+DL AEFYLETV++VFQ+HALPLG++ R +EP AIRRTALFT+EGEKDDI Sbjct: 384 YAEYFAMSDLAAEFYLETVQSVFQDHALPLGEMQAFGRTIEPSAIRRTALFTVEGEKDDI 443 Query: 375 CAVGQTVAAQEMCSNIRPYMRLHHVQTAVGHYGVFNGRRWDNEIYPRLRDFINMHH 430 CAVGQTVAAQ+MCS IRPYM+ HHVQT VGHYGVFNGRRWDNEIYPRLRDFI+ H Sbjct: 444 CAVGQTVAAQDMCSGIRPYMKSHHVQTGVGHYGVFNGRRWDNEIYPRLRDFIHSHQ 499 >ref|YP_001242104.1| hypothetical protein BBta_6279 [Bradyrhizobium sp. BTAi1] gb|ABQ38198.1| hypothetical protein BBta_6279 [Bradyrhizobium sp. BTAi1] Length = 406 Score = 502 bits (1293), Expect = e-140, Method: Composition-based stats. Identities = 236/402 (58%), Positives = 308/402 (76%), Gaps = 1/402 (0%) Query: 28 YQLYQNYADATDPLRACARMMAPLLGATWPGVPVNPALRKMASACEVFARTQLTHARPPF 87 YQ YQN+ D T+P R A L G+ + A+R++++A E+ +R+ LT+ARP + Sbjct: 5 YQAYQNHMDLTEPWRQGAAAALRYLNLIPSGLS-SRAMRRLSAALELISRSALTYARPAY 63 Query: 88 EIGEVLDGGRTIAVHEEEVSATPFCGLLHFRKESSAVQPKVLVVAPMSGHFATLLRGTVK 147 I V+ G R +AV EE TPF LLHF+K+++ QP+VL+VAPMSGHFATLLRGTVK Sbjct: 64 GIDSVMVGNREVAVTEEVTFKTPFGSLLHFKKDAAPEQPRVLLVAPMSGHFATLLRGTVK 123 Query: 148 TLLRDHDVYITDWRNARDVAVEHGRFGLDEYVSHVIDFLDMLGPGAHLLAVCQPTVAALT 207 TLL+DHDVYITDW N RD+ +HGRFGL++Y H+I FL LGP H++A+CQP+V+AL Sbjct: 124 TLLQDHDVYITDWHNPRDIPRDHGRFGLEDYTDHLISFLGQLGPRPHMIAICQPSVSALA 183 Query: 208 AASVMAEDNHPAQPRTLTLMAGPLDTRVNPTAVNALAKSKPISWFEKNMISTVPARHGGA 267 A +VM EDNHP++P TLTLMAGP+DTR+ PT VN AKSKPI WFE N+I VP + GA Sbjct: 184 ATAVMCEDNHPSRPATLTLMAGPIDTRIQPTKVNEFAKSKPIGWFEDNLIHYVPFQCRGA 243 Query: 268 GRRVYPGFVQLSAFMNMNLNRHVDAFRKLYHHLVEGEEAQAAQIKDFYEEYFAMADLPAE 327 R+VYPGFVQL+AF++MNL RHV L +HL +GE +A IK FY+EYFA+ DLPAE Sbjct: 244 FRKVYPGFVQLTAFVSMNLERHVKQHMDLANHLAKGEVEKAEIIKTFYDEYFAVMDLPAE 303 Query: 328 FYLETVRTVFQEHALPLGKLTYRERVVEPRAIRRTALFTIEGEKDDICAVGQTVAAQEMC 387 FY+ETVR VFQEH LP GKL +R R V+P+A+RR L T+EGE+DDICA+GQT+AAQ++C Sbjct: 304 FYIETVRDVFQEHLLPQGKLMHRGRPVDPKAVRRIGLMTVEGERDDICAIGQTLAAQDIC 363 Query: 388 SNIRPYMRLHHVQTAVGHYGVFNGRRWDNEIYPRLRDFINMH 429 +N+R Y R+HH+Q VGHYGVF+G++W+NEIYP LRDF++++ Sbjct: 364 TNVRAYRRIHHMQAGVGHYGVFSGKKWNNEIYPLLRDFVHVN 405 >ref|ZP_06841365.1| polyhydroxyalkanoate depolymerase, intracellular [Burkholderia sp. Ch1-1] gb|EFG70891.1| polyhydroxyalkanoate depolymerase, intracellular [Burkholderia sp. Ch1-1] Length = 503 Score = 499 bits (1285), Expect = e-139, Method: Composition-based stats. Identities = 252/410 (61%), Positives = 305/410 (74%), Gaps = 3/410 (0%) Query: 19 RGRRFMINTYQLYQNYADATDPLRACARMMAPLLGATWPGVPVNPALRKMASACEVFART 78 R + + Y YQ +AD P+R A +M L A WP P R + +ACE+ Sbjct: 14 RAKVMNLFAYPTYQAFADMMLPMRHGAALMNHSLDA-WPAFGETPHGRSIRAACELLTLA 72 Query: 79 QLTHARPPFEIGEVLDGGRTIAVHEEEVSATPFCGLLHFRKES--SAVQPKVLVVAPMSG 136 LT RPPF I V+ G+++A+ EE + TPFC LLHFRKE+ S QP+VLV+APMSG Sbjct: 73 GLTPVRPPFGIDSVVTEGKSVAIVEEVAAHTPFCSLLHFRKETQPSVPQPRVLVIAPMSG 132 Query: 137 HFATLLRGTVKTLLRDHDVYITDWRNARDVAVEHGRFGLDEYVSHVIDFLDMLGPGAHLL 196 HFATLLRGTV+T+L +HDVYITDW N RDV + GRFG DE+V HVIDF + +GPGAHLL Sbjct: 133 HFATLLRGTVRTMLAEHDVYITDWHNPRDVPLSQGRFGFDEFVQHVIDFTETMGPGAHLL 192 Query: 197 AVCQPTVAALTAASVMAEDNHPAQPRTLTLMAGPLDTRVNPTAVNALAKSKPISWFEKNM 256 AVCQPTVAAL A ++MA D+HPAQP ++TLMAGPLDTR+NPT VN LAKSKPI WFE+N+ Sbjct: 193 AVCQPTVAALAAVALMAADDHPAQPASMTLMAGPLDTRINPTRVNDLAKSKPIEWFEQNL 252 Query: 257 ISTVPARHGGAGRRVYPGFVQLSAFMNMNLNRHVDAFRKLYHHLVEGEEAQAAQIKDFYE 316 IS VP GA RRVYPGFVQL+AFM MNLNRH+D+F +Y+ +G+ A+A I FYE Sbjct: 253 ISAVPFGFAGAHRRVYPGFVQLTAFMAMNLNRHLDSFETMYYERAKGDPAKADTIHTFYE 312 Query: 317 EYFAMADLPAEFYLETVRTVFQEHALPLGKLTYRERVVEPRAIRRTALFTIEGEKDDICA 376 EYFA DL A+FYLETV TVFQ HALPL L + R+VEP IRRTAL T+EGEKDDICA Sbjct: 313 EYFATMDLTADFYLETVDTVFQRHALPLHALEVKGRLVEPSRIRRTALLTVEGEKDDICA 372 Query: 377 VGQTVAAQEMCSNIRPYMRLHHVQTAVGHYGVFNGRRWDNEIYPRLRDFI 426 VGQT+AAQ+MC +RPY++ HHVQT VGHYGVFNG RW+ ++YPR+R I Sbjct: 373 VGQTLAAQDMCDKLRPYLKTHHVQTGVGHYGVFNGHRWERQVYPRVRAVI 422 >ref|YP_552404.1| polyhydroxyalkanoate depolymerase [Burkholderia xenovorans LB400] gb|ABE33054.1| Polyhydroxyalkanoate depolymerase [Burkholderia xenovorans LB400] Length = 492 Score = 495 bits (1275), Expect = e-138, Method: Composition-based stats. Identities = 250/401 (62%), Positives = 302/401 (75%), Gaps = 3/401 (0%) Query: 28 YQLYQNYADATDPLRACARMMAPLLGATWPGVPVNPALRKMASACEVFARTQLTHARPPF 87 Y YQ +AD P+R A +M L A WP P R + +ACE+ LT RPPF Sbjct: 6 YPTYQAFADMMLPMRHGAALMNHSLDA-WPAFGETPHGRSIRAACELLTLAGLTPVRPPF 64 Query: 88 EIGEVLDGGRTIAVHEEEVSATPFCGLLHFRKES--SAVQPKVLVVAPMSGHFATLLRGT 145 + V+ G+++A+ EE + TPFC LLHFRKE+ S QP+VLV+APMSGHFATLLRGT Sbjct: 65 GLDSVVTEGKSVAIVEEVAAHTPFCSLLHFRKETPPSVPQPRVLVIAPMSGHFATLLRGT 124 Query: 146 VKTLLRDHDVYITDWRNARDVAVEHGRFGLDEYVSHVIDFLDMLGPGAHLLAVCQPTVAA 205 V+T+L +HDVYITDW N RDV + GRFG DE+V HVIDF + +GPGAHLLAVCQPTVAA Sbjct: 125 VRTMLAEHDVYITDWHNPRDVPLSQGRFGFDEFVQHVIDFTETIGPGAHLLAVCQPTVAA 184 Query: 206 LTAASVMAEDNHPAQPRTLTLMAGPLDTRVNPTAVNALAKSKPISWFEKNMISTVPARHG 265 L A ++MA D+HPAQP ++TLMAGPLDTR+NPT VN LAKSKPI WFE+N+IS VP Sbjct: 185 LAAVALMAADDHPAQPASMTLMAGPLDTRINPTRVNDLAKSKPIEWFEQNLISAVPFGFA 244 Query: 266 GAGRRVYPGFVQLSAFMNMNLNRHVDAFRKLYHHLVEGEEAQAAQIKDFYEEYFAMADLP 325 GA RRVYPGFVQL+AFM MNLNRH+D+F +Y+ +G+ A+A I FYEEYFA DL Sbjct: 245 GAHRRVYPGFVQLTAFMAMNLNRHLDSFETMYYERAKGDPAKADTIHTFYEEYFATMDLT 304 Query: 326 AEFYLETVRTVFQEHALPLGKLTYRERVVEPRAIRRTALFTIEGEKDDICAVGQTVAAQE 385 A+FYLETV TVFQ HALPL L + R+VEP IRRTAL T+EGEKDDICAVGQT+AAQ+ Sbjct: 305 ADFYLETVDTVFQRHALPLHALEVKGRLVEPSRIRRTALLTVEGEKDDICAVGQTLAAQD 364 Query: 386 MCSNIRPYMRLHHVQTAVGHYGVFNGRRWDNEIYPRLRDFI 426 MC +RPY++ HHVQT VGHYGVFNG RW+ ++YPR+R I Sbjct: 365 MCDKLRPYLKTHHVQTGVGHYGVFNGHRWERQVYPRVRAVI 405