BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.0644.p (328 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003773889.1| carboxymethylenebutenolidase [Herbaspirillum... 588 e-166 ref|YP_001098627.1| putative carboxymethylenebutenolidase [Hermi... 429 e-118 ref|YP_004751384.1| putative carboxymethylenebutenolidase (Diene... 422 e-116 ref|YP_001352014.1| carboxymethylenebutenolidase [Janthinobacter... 422 e-116 ref|ZP_08275791.1| putative carboxymethylenebutenolidase (Dienel... 387 e-105 >ref|YP_003773889.1| carboxymethylenebutenolidase [Herbaspirillum seropedicae SmR1] gb|ADJ61981.1| carboxymethylenebutenolidase (dienelactone hydrolase) protein [Herbaspirillum seropedicae SmR1] Length = 294 Score = 588 bits (1517), Expect = e-166, Method: Composition-based stats. Identities = 284/294 (96%), Positives = 291/294 (98%) Query: 35 MNDLRNDFNSLLGQSNLSGNADRRTFLKTALGTGFAAAVLPVCAQTMIKTDTQGLTAGEV 94 MNDLRNDFNSLLGQS LSGNADRR+FLKTALGTGFAAAVLPVCAQTMIKTDTQGLTAGEV Sbjct: 1 MNDLRNDFNSLLGQSTLSGNADRRSFLKTALGTGFAAAVLPVCAQTMIKTDTQGLTAGEV 60 Query: 95 SIEVNGQRVPAYRAQPEGKTGLPVIIVVSEIFGVHEHIADIARRFAKLGYLAIAPDFFVR 154 SIEVNGQRVPAYRAQPE KTGLPVIIVVSEIFGVHEHIADIARRFAKLGYLAIAPDFFVR Sbjct: 61 SIEVNGQRVPAYRAQPERKTGLPVIIVVSEIFGVHEHIADIARRFAKLGYLAIAPDFFVR 120 Query: 155 QGNPMAYTSIAELQKEIISKVPDAQVMGDIDAYVKWAGENGGDIKRLGINGFCWGGRIVW 214 QGNPMAYTSIAELQK+IISKVPDAQVMGDIDAYVKWAGE+GGDI+RLGINGFCWGGRIVW Sbjct: 121 QGNPMAYTSIAELQKDIISKVPDAQVMGDIDAYVKWAGEHGGDIRRLGINGFCWGGRIVW 180 Query: 215 LYSAHNPQVKAGAAWYGRLVGDKTALFPQQPIDIAPTLKTPVIGLYGGKDQGIPVSTVEQ 274 LYSAHNP+VKAGAAWYGRLVG+K+ LFPQQPIDIAPTLKTPVIGLYGGKDQGIPVSTVEQ Sbjct: 181 LYSAHNPKVKAGAAWYGRLVGEKSELFPQQPIDIAPTLKTPVIGLYGGKDQGIPVSTVEQ 240 Query: 275 MKEALQKAGNKSEFHVYMNSGHAFNADYRPSYVEADAKDAWSKVVAFFKQHGVA 328 MKEALQKAGNKSEFHVYMNSGHAFNADYRPSYVEADAKDAWSKVVAFFKQHGVA Sbjct: 241 MKEALQKAGNKSEFHVYMNSGHAFNADYRPSYVEADAKDAWSKVVAFFKQHGVA 294 >ref|YP_001098627.1| putative carboxymethylenebutenolidase [Herminiimonas arsenicoxydans] emb|CAL60498.1| putative carboxymethylenebutenolidase [Herminiimonas arsenicoxydans] Length = 294 Score = 429 bits (1103), Expect = e-118, Method: Composition-based stats. Identities = 206/294 (70%), Positives = 242/294 (82%) Query: 35 MNDLRNDFNSLLGQSNLSGNADRRTFLKTALGTGFAAAVLPVCAQTMIKTDTQGLTAGEV 94 M +L+ DF+SL+ ++ LS +RR F+KTALGTGFAAAVLPV AQ +IKTD GLTAGE+ Sbjct: 1 MKNLQEDFDSLVAKTPLSDGVNRRGFIKTALGTGFAAAVLPVSAQHVIKTDDAGLTAGEI 60 Query: 95 SIEVNGQRVPAYRAQPEGKTGLPVIIVVSEIFGVHEHIADIARRFAKLGYLAIAPDFFVR 154 ++ VNGQ VP YRAQP+GKT LPVI+V+SEIFGVHE+IAD+ARRFAKLGYLA+AP+ FVR Sbjct: 61 TVNVNGQAVPVYRAQPKGKTDLPVILVISEIFGVHEYIADVARRFAKLGYLALAPELFVR 120 Query: 155 QGNPMAYTSIAELQKEIISKVPDAQVMGDIDAYVKWAGENGGDIKRLGINGFCWGGRIVW 214 QG+P +Y +IAELQKEIISKVPDAQVM D+DA V WA NGG+ +LGI GFCWGGRI W Sbjct: 121 QGDPGSYGTIAELQKEIISKVPDAQVMTDLDAVVAWAKANGGNTGKLGITGFCWGGRITW 180 Query: 215 LYSAHNPQVKAGAAWYGRLVGDKTALFPQQPIDIAPTLKTPVIGLYGGKDQGIPVSTVEQ 274 LY+AHNPQ+KAG AWYGRLVGD T L P+ PIDIA LK PV+GLYG +D GIPVSTV Q Sbjct: 181 LYAAHNPQIKAGVAWYGRLVGDSTPLTPKNPIDIAAKLKAPVLGLYGAQDGGIPVSTVVQ 240 Query: 275 MKEALQKAGNKSEFHVYMNSGHAFNADYRPSYVEADAKDAWSKVVAFFKQHGVA 328 MKEAL K +KSEF V+ NSGHAF+ADYR SYVEADAK+ W + +FK HGV Sbjct: 241 MKEALAKGSSKSEFIVFKNSGHAFHADYRASYVEADAKEGWQHAITWFKTHGVG 294 >ref|YP_004751384.1| putative carboxymethylenebutenolidase (Dienelactone hydrolase) [Collimonas fungivorans Ter331] gb|AEK60561.1| putative carboxymethylenebutenolidase (Dienelactone hydrolase) [Collimonas fungivorans Ter331] Length = 305 Score = 422 bits (1085), Expect = e-116, Method: Composition-based stats. Identities = 206/304 (67%), Positives = 242/304 (79%), Gaps = 10/304 (3%) Query: 35 MNDLRNDFNSLLGQSNLSGNADRRTFLKTALGTGFAAAVLPVCAQTMIKTDTQGLTAGEV 94 M +L+ DF+SL+ +++LS DRRTF+KTALGTGFAAAVLPV AQT+IKTDT+GLTAG + Sbjct: 2 MPNLKEDFDSLVAKTSLSDGVDRRTFMKTALGTGFAAAVLPVTAQTVIKTDTEGLTAGAL 61 Query: 95 SIEVNGQRVPAYRAQPEGKTGLPVIIVVSEIFGVHEHIADIARRFAKLGYLAIAPDFFVR 154 IE NG VP YRAQPEGKT LPV++VVSEIFGVHEHIADIARRFAKLGYLAIAP+ F R Sbjct: 62 DIEENGLSVPIYRAQPEGKTNLPVVLVVSEIFGVHEHIADIARRFAKLGYLAIAPNLFKR 121 Query: 155 QGNPMAYTSIAELQKEIISKVPDAQVMGDIDAYVKWAGENGGDIKRLGINGFCWGGRIVW 214 QG+P A+ SI EL +ISKVPD QV+GD+DA V WA +NGG++ +L + GFCWGGRI W Sbjct: 122 QGDPAAFASIPELYVNVISKVPDDQVLGDLDACVAWAKKNGGNVNKLAVTGFCWGGRITW 181 Query: 215 LYSAHNPQVKAGAAWYGRLVGDK----------TALFPQQPIDIAPTLKTPVIGLYGGKD 264 LY++HNP+VKA AAWYG LV K AL P+QP+DIAPTLK PV+GLYG KD Sbjct: 182 LYASHNPKVKAAAAWYGSLVRGKDPEGKTEAEIKALTPKQPVDIAPTLKVPVLGLYGAKD 241 Query: 265 QGIPVSTVEQMKEALQKAGNKSEFHVYMNSGHAFNADYRPSYVEADAKDAWSKVVAFFKQ 324 QGI V QMK AL K +KSE VY NSGHAFNADYRPSYVEADAKD W +V+A+FK+ Sbjct: 242 QGITQDHVAQMKAALAKGSSKSEIVVYPNSGHAFNADYRPSYVEADAKDGWKRVLAWFKE 301 Query: 325 HGVA 328 HGV+ Sbjct: 302 HGVS 305 >ref|YP_001352014.1| carboxymethylenebutenolidase [Janthinobacterium sp. Marseille] gb|ABR88829.1| carboxymethylenebutenolidase [Janthinobacterium sp. Marseille] Length = 294 Score = 422 bits (1084), Expect = e-116, Method: Composition-based stats. Identities = 193/294 (65%), Positives = 242/294 (82%) Query: 35 MNDLRNDFNSLLGQSNLSGNADRRTFLKTALGTGFAAAVLPVCAQTMIKTDTQGLTAGEV 94 M++L+ DF+SL+ ++ +S DRR FLKTALG+GFAAAVLP+ A + + TD++GLT GE+ Sbjct: 1 MDNLKEDFDSLVAKTPISDGVDRRGFLKTALGSGFAAAVLPIAAHSAVTTDSKGLTVGEI 60 Query: 95 SIEVNGQRVPAYRAQPEGKTGLPVIIVVSEIFGVHEHIADIARRFAKLGYLAIAPDFFVR 154 ++ VNGQ VP YRAQP GKT LPV++V+SEIFGVHE+IAD+ARRFAKLGYLA+AP+ FVR Sbjct: 61 TVNVNGQAVPVYRAQPAGKTNLPVVLVISEIFGVHEYIADVARRFAKLGYLALAPELFVR 120 Query: 155 QGNPMAYTSIAELQKEIISKVPDAQVMGDIDAYVKWAGENGGDIKRLGINGFCWGGRIVW 214 QG+P +Y +IAELQKEIISKVPDAQVM D+DA V WA NGG+ +LGI GFCWGGR+ W Sbjct: 121 QGDPGSYGTIAELQKEIISKVPDAQVMTDLDAVVAWAKANGGNTDKLGITGFCWGGRVTW 180 Query: 215 LYSAHNPQVKAGAAWYGRLVGDKTALFPQQPIDIAPTLKTPVIGLYGGKDQGIPVSTVEQ 274 LYSAHNP++KAG AWYGRLVG+ L P P+DIA LKTPV+GLYG +D GIPV+TV Q Sbjct: 181 LYSAHNPKIKAGVAWYGRLVGNNNELTPNNPVDIAAKLKTPVLGLYGAQDGGIPVTTVVQ 240 Query: 275 MKEALQKAGNKSEFHVYMNSGHAFNADYRPSYVEADAKDAWSKVVAFFKQHGVA 328 M++AL K +KSEF ++ NSGHAF+ADYR SYVEADAK+ W + + +FK HGV+ Sbjct: 241 MQDALSKGKSKSEFVLFKNSGHAFHADYRSSYVEADAKEGWQRCIDWFKTHGVS 294 >ref|ZP_08275791.1| putative carboxymethylenebutenolidase (Dienelactone hydrolase) [Oxalobacteraceae bacterium IMCC9480] gb|EGF30734.1| putative carboxymethylenebutenolidase (Dienelactone hydrolase) [Oxalobacteraceae bacterium IMCC9480] Length = 295 Score = 387 bits (993), Expect = e-105, Method: Composition-based stats. Identities = 186/290 (64%), Positives = 224/290 (77%) Query: 39 RNDFNSLLGQSNLSGNADRRTFLKTALGTGFAAAVLPVCAQTMIKTDTQGLTAGEVSIEV 98 + D SL+G S DRRTF+K ++GTGFAAAV+PV AQTM+ TDT GLTAG +S+ V Sbjct: 6 KQDAASLIGASAFPDGFDRRTFMKVSIGTGFAAAVMPVSAQTMVMTDTDGLTAGTISLTV 65 Query: 99 NGQRVPAYRAQPEGKTGLPVIIVVSEIFGVHEHIADIARRFAKLGYLAIAPDFFVRQGNP 158 NG++VP YRAQP GKT LPVI+V+SEIFGVHEHIAD+ARRFAKLGYLA+APD FVRQG+ Sbjct: 66 NGEQVPVYRAQPAGKTKLPVILVISEIFGVHEHIADVARRFAKLGYLALAPDLFVRQGDA 125 Query: 159 MAYTSIAELQKEIISKVPDAQVMGDIDAYVKWAGENGGDIKRLGINGFCWGGRIVWLYSA 218 A SI EL K ++SKVPD QVM D+DA V +A NGGD++R+GI GFCWGGRI WLYSA Sbjct: 126 KAAISIPELIKNVVSKVPDQQVMTDLDAVVAYAKANGGDVERMGITGFCWGGRITWLYSA 185 Query: 219 HNPQVKAGAAWYGRLVGDKTALFPQQPIDIAPTLKTPVIGLYGGKDQGIPVSTVEQMKEA 278 HNPQV+AG AWYG+L G K+AL P ++AP L PV+GLYG KD GI ++ MK A Sbjct: 186 HNPQVRAGVAWYGQLQGTKSALNPMSATELAPALTVPVLGLYGAKDTGISQDSIATMKAA 245 Query: 279 LQKAGNKSEFHVYMNSGHAFNADYRPSYVEADAKDAWSKVVAFFKQHGVA 328 L ++SEF V+ NSGHAF+ADYR SYVEADAK+ W K +A+F HGVA Sbjct: 246 LATGKSQSEFVVFPNSGHAFHADYRASYVEADAKEGWKKCLAWFAAHGVA 295