BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.0496.p (455 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003773968.1| major facilitator superfamily permease [Herb... 700 0.0 ref|YP_582496.1| major facilitator superfamily transporter [Cupr... 582 e-164 ref|YP_298417.1| major facilitator transporter [Ralstonia eutrop... 574 e-162 ref|YP_001860217.1| major facilitator transporter [Burkholderia ... 562 e-158 ref|YP_004681740.1| ACS family major facilitator superfamily pro... 559 e-157 >ref|YP_003773968.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] gb|ADJ62060.1| permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 449 Score = 700 bits (1806), Expect = 0.0, Method: Composition-based stats. Identities = 405/445 (91%), Positives = 420/445 (94%), Gaps = 2/445 (0%) Query: 2 MTPSPGAGTLAAPSVLKLSVAAEDAIYRKVWLRIIPFLFICYVVSFLDRINIGFAQLQMK 61 MTPS G TL+ + L+ AEDAIYRKVWLRIIPFLFICYVVSFLDRINIGFAQLQMK Sbjct: 1 MTPSSGGATLS--TTPSLASQAEDAIYRKVWLRIIPFLFICYVVSFLDRINIGFAQLQMK 58 Query: 62 QDLGFSDAMYGLGAAVFYVGYVLCEVPSNMLLARFGARRTFSRIMVLWGLASVGMMAVSD 121 QDLGFSDAMYGLGAA+FYVGYVL EVPSNM LARFGARRTFSRIMVLWGLASVGMMAV+D Sbjct: 59 QDLGFSDAMYGLGAAIFYVGYVLFEVPSNMALARFGARRTFSRIMVLWGLASVGMMAVTD 118 Query: 122 ATHFYVLRFLLGVFEAGFFPGIVLYLSYWFPARRRAAVMAIFFAGVAVAGVLGGLVSGWI 181 A HFY LRFLLGVFEAGFFPGIVLYLSYWFPARRRAAVMAIFFAGVAVAGVLGGLVSGWI Sbjct: 119 AAHFYTLRFLLGVFEAGFFPGIVLYLSYWFPARRRAAVMAIFFAGVAVAGVLGGLVSGWI 178 Query: 182 MRDMAGVMGLYGWQWMFAIEGAPAVVLGVLVIFLLADGPAQATWLTPEERSFLQNRQSQE 241 MRDMAGVMGLYGWQWMFAIEGAPAVVLG+L IFLLADGPAQA WL+PEERSFLQ R QE Sbjct: 179 MRDMAGVMGLYGWQWMFAIEGAPAVVLGLLAIFLLADGPAQAKWLSPEERSFLQARHQQE 238 Query: 242 ALRTHHVSAHSLTALKQALSHRPTYLFAFIYFALTCGSLTLNFWMPLMIRDFGIKDVVQV 301 ALRTHHVS HSL AL+QALSHR TYLFAFIYFALTCGSLTLNFWMPLMIRDFGIKDVV+V Sbjct: 239 ALRTHHVSGHSLAALRQALSHRTTYLFAFIYFALTCGSLTLNFWMPLMIRDFGIKDVVEV 298 Query: 302 SLYTVIPNAIGAIGLILIARRSDRRNERHGHFLVCTTGGALALAALTLHPSSFAVSLGLL 361 SLY+VIPNAIGA+GLILIARRSDRRNERHGHF VCTTGGALALAALTLHPSSFAVSLGLL Sbjct: 299 SLYSVIPNAIGAVGLILIARRSDRRNERHGHFAVCTTGGALALAALTLHPSSFAVSLGLL 358 Query: 362 SVAAVLIFAALPIFWSLPPGLLPSQTIAPGIAFISSVGITSGIVSPWLIGLVKTRTGNID 421 SVAAVLIFAALPIFWSLPPGLLP+QTIAPGIAFISSVGITSGIVSPWLIGLVKTRTGN+D Sbjct: 359 SVAAVLIFAALPIFWSLPPGLLPAQTIAPGIAFISSVGITSGIVSPWLIGLVKTRTGNMD 418 Query: 422 IALYILAGLLLLSGLTMWTMIPRKG 446 IALYILAGLLLLSGL MW++IPRK Sbjct: 419 IALYILAGLLLLSGLAMWSLIPRKA 443 >ref|YP_582496.1| major facilitator superfamily transporter [Cupriavidus metallidurans CH34] gb|ABF07227.1| Major facilitator superfamily (MFS_1) transporter [Cupriavidus metallidurans CH34] Length = 453 Score = 582 bits (1501), Expect = e-164, Method: Composition-based stats. Identities = 339/439 (77%), Positives = 376/439 (85%), Gaps = 3/439 (0%) Query: 6 PGAGTLAAPSVLKLSVAAEDAIYRKVWLRIIPFLFICYVVSFLDRINIGFAQLQMKQDLG 65 P + A P VL EDA+YRKVWLRIIPFLFICYVVSFLDRINIGFAQLQMK DLG Sbjct: 13 PEIPSHAIPQVL--PTQQEDALYRKVWLRIIPFLFICYVVSFLDRINIGFAQLQMKHDLG 70 Query: 66 FSDAMYGLGAAVFYVGYVLCEVPSNMLLARFGARRTFSRIMVLWGLASVGMMAVSDATHF 125 FSDAMYGLGAAVFYVGYVLCEVPSNMLLARFGARRTF+RIM+LWG+ASVGMM VS +HF Sbjct: 71 FSDAMYGLGAAVFYVGYVLCEVPSNMLLARFGARRTFTRIMLLWGVASVGMMLVSQPSHF 130 Query: 126 YVLRFLLGVFEAGFFPGIVLYLSYWFPARRRAAVMAIFFAGVAVAGVLGGLVSGWIMRDM 185 Y+LRFLLGVFEAGFFPGIVLYL+YWFPARRRAAVMAIFFAGVAVAGVLGGL+SGWIMRDM Sbjct: 131 YLLRFLLGVFEAGFFPGIVLYLTYWFPARRRAAVMAIFFAGVAVAGVLGGLISGWIMRDM 190 Query: 186 AGVMGLYGWQWMFAIEGAPAVVLGVLVIFLLADGPAQATWLTPEERSFLQNRQSQEALRT 245 AGV+G+ GWQWMFAIEGAPAV+LG++ L DGP QA WLTP E+ +L + E+ R Sbjct: 191 AGVLGMRGWQWMFAIEGAPAVLLGLIAAVCLVDGPRQARWLTPAEQDYLVAQHEAES-RG 249 Query: 246 HHVSAHSLTALKQALSHRPTYLFAFIYFALTCGSLTLNFWMPLMIRDFGIKDVVQVSLYT 305 AHS+ AL+QAL++ YLFAFIYFALTCGSLTL+FWMPLMIRDFGI+DVV VSLY+ Sbjct: 250 AGGHAHSMQALRQALANPRVYLFAFIYFALTCGSLTLSFWMPLMIRDFGIQDVVAVSLYS 309 Query: 306 VIPNAIGAIGLILIARRSDRRNERHGHFLVCTTGGALALAALTLHPSSFAVSLGLLSVAA 365 VIPNAIGA+GLILIARRSDR ERHGHFL CT GGALALAALTLH S VSL +LSVAA Sbjct: 310 VIPNAIGAVGLILIARRSDRLGERHGHFLCCTAGGALALAALTLHVPSLGVSLAILSVAA 369 Query: 366 VLIFAALPIFWSLPPGLLPSQTIAPGIAFISSVGITSGIVSPWLIGLVKTRTGNIDIALY 425 VLIFAALPIFW+LPP LP+QT A GIAFISS+GITSGIVSPW+IG +K TG++D ALY Sbjct: 370 VLIFAALPIFWALPPSHLPAQTAATGIAFISSIGITSGIVSPWVIGQIKAHTGSMDNALY 429 Query: 426 ILAGLLLLSGLTMWTMIPR 444 +LAGLLL SGL +W +PR Sbjct: 430 LLAGLLLASGLALWKGVPR 448 >ref|YP_298417.1| major facilitator transporter [Ralstonia eutropha JMP134] gb|AAZ63573.1| General substrate transporter:Major facilitator superfamily MFS_1 [Ralstonia eutropha JMP134] Length = 440 Score = 574 bits (1480), Expect = e-162, Method: Composition-based stats. Identities = 321/423 (75%), Positives = 366/423 (86%), Gaps = 4/423 (0%) Query: 24 EDAIYRKVWLRIIPFLFICYVVSFLDRINIGFAQLQMKQDLGFSDAMYGLGAAVFYVGYV 83 EDA+YRK+WLR+IPFLF+CYVVSFLDRINIGFAQLQMKQDLGFSDAMYGLGAAVFYVGYV Sbjct: 19 EDALYRKIWLRLIPFLFVCYVVSFLDRINIGFAQLQMKQDLGFSDAMYGLGAAVFYVGYV 78 Query: 84 LCEVPSNMLLARFGARRTFSRIMVLWGLASVGMMAVSDATHFYVLRFLLGVFEAGFFPGI 143 LCEVPSNMLLARFGARRTF+RIMVLWG+ASVGMMAVS T FY LRFLLGVFEAGFFPGI Sbjct: 79 LCEVPSNMLLARFGARRTFTRIMVLWGVASVGMMAVSSPTQFYTLRFLLGVFEAGFFPGI 138 Query: 144 VLYLSYWFPARRRAAVMAIFFAGVAVAGVLGGLVSGWIMRDMAGVMGLYGWQWMFAIEGA 203 VLYL+YWFPA+RRAAVMAIFFAGVA+AGVLGGLVSGWIMRDMAGV G+ GW+WMFAIEGA Sbjct: 139 VLYLTYWFPAKRRAAVMAIFFAGVAIAGVLGGLVSGWIMRDMAGVFGMQGWKWMFAIEGA 198 Query: 204 PAVVLGVLVIFLLADGPAQATWLTPEERSFLQNRQSQEALRTHHVSAHSLTALKQALSHR 263 PA+VLGV+ F L D P QA+WL+ E++FL+ ++Q+ H V AHSL AL Q L + Sbjct: 199 PAIVLGVIAAFFLVDEPRQASWLSDGEKAFLERARAQD----HGVKAHSLQALLQVLRNP 254 Query: 264 PTYLFAFIYFALTCGSLTLNFWMPLMIRDFGIKDVVQVSLYTVIPNAIGAIGLILIARRS 323 YLFAFIYF+LTCGSL L+FWMPLMIRDFGI DVV +SLY+V+PNA+GA+GLILIAR S Sbjct: 255 RVYLFAFIYFSLTCGSLALSFWMPLMIRDFGITDVVSISLYSVVPNAVGAVGLILIARHS 314 Query: 324 DRRNERHGHFLVCTTGGALALAALTLHPSSFAVSLGLLSVAAVLIFAALPIFWSLPPGLL 383 DR ERH HFL+CT GGALALAALTLH S A L +LS+AAVLIFAALP+FWSLPP L Sbjct: 315 DRSGERHRHFLLCTVGGALALAALTLHLQSLAAMLAILSMAAVLIFAALPVFWSLPPSHL 374 Query: 384 PSQTIAPGIAFISSVGITSGIVSPWLIGLVKTRTGNIDIALYILAGLLLLSGLTMWTMIP 443 P+ A GIAFISSVGITSGIVSPW+IG +K +TG++D ALY+LA LL+ SG++MW +P Sbjct: 375 PASGAAAGIAFISSVGITSGIVSPWVIGQIKVQTGSLDNALYLLAALLVASGMSMWLGVP 434 Query: 444 RKG 446 R+ Sbjct: 435 RQA 437 >ref|YP_001860217.1| major facilitator transporter [Burkholderia phymatum STM815] gb|ACC73171.1| major facilitator superfamily MFS_1 [Burkholderia phymatum STM815] Length = 442 Score = 562 bits (1448), Expect = e-158, Method: Composition-based stats. Identities = 301/423 (71%), Positives = 353/423 (83%), Gaps = 3/423 (0%) Query: 24 EDAIYRKVWLRIIPFLFICYVVSFLDRINIGFAQLQMKQDLGFSDAMYGLGAAVFYVGYV 83 + A+YRKV LRI+PFLF+CYV+SFLDRINIGFAQLQMK DLGFSDAMYGLGAAVFY+GYV Sbjct: 21 DTALYRKVTLRIVPFLFLCYVISFLDRINIGFAQLQMKHDLGFSDAMYGLGAAVFYIGYV 80 Query: 84 LCEVPSNMLLARFGARRTFSRIMVLWGLASVGMMAVSDATHFYVLRFLLGVFEAGFFPGI 143 LCEVPSNMLLARFGARRTF+RIM+LWG ASV MM VS +HFYVLRF+LGVFEAGFFPGI Sbjct: 81 LCEVPSNMLLARFGARRTFTRIMLLWGAASVCMMFVSQPSHFYVLRFMLGVFEAGFFPGI 140 Query: 144 VLYLSYWFPARRRAAVMAIFFAGVAVAGVLGGLVSGWIMRDMAGVMGLYGWQWMFAIEGA 203 VLYL+YW+PA+RRA +++IFFAGVAVAGVLGGLVSGWIMRDMAGVMGLYGWQWMFAIEGA Sbjct: 141 VLYLTYWYPAQRRAVILSIFFAGVAVAGVLGGLVSGWIMRDMAGVMGLYGWQWMFAIEGA 200 Query: 204 PAVVLGVLVIFLLADGPAQATWLTPEERSFLQNRQSQEALRTHHVSAHSLTALKQALSHR 263 PA+V G+L F L DGP +A WLT +E++ L ++ E ++HS A AL + Sbjct: 201 PALVCGLLAAFWLVDGPQEAHWLTQQEKAHLVAQRDGEH---RPANSHSSRAFVDALRNP 257 Query: 264 PTYLFAFIYFALTCGSLTLNFWMPLMIRDFGIKDVVQVSLYTVIPNAIGAIGLILIARRS 323 YLFAFIYF+LTC SLTLNFWMPLMIRDFG++DV+ +SLYTVIPNAIGA+GLILIAR S Sbjct: 258 RVYLFAFIYFSLTCASLTLNFWMPLMIRDFGVRDVLWISLYTVIPNAIGAVGLILIARHS 317 Query: 324 DRRNERHGHFLVCTTGGALALAALTLHPSSFAVSLGLLSVAAVLIFAALPIFWSLPPGLL 383 DR ER HF CT GG +AL+ LTLH SSFA LG+LS+AAVLIFAALPIFW++P G L Sbjct: 318 DRHGERRKHFAACTIGGGVALSLLTLHLSSFAAMLGILSIAAVLIFAALPIFWTVPSGYL 377 Query: 384 PSQTIAPGIAFISSVGITSGIVSPWLIGLVKTRTGNIDIALYILAGLLLLSGLTMWTMIP 443 + A GI ISS+GITSGI+SPW+IGL+KTRTG++D ALY+L GLL LSG+ + +P Sbjct: 378 SGKAAAAGIGLISSIGITSGILSPWVIGLIKTRTGSMDNALYVLTGLLFLSGIALLRGVP 437 Query: 444 RKG 446 ++ Sbjct: 438 KRA 440 >ref|YP_004681740.1| ACS family major facilitator superfamily protein [Cupriavidus necator N-1] gb|AEI80508.1| major facilitator superfamily MFS ACS family [Cupriavidus necator N-1] Length = 452 Score = 559 bits (1441), Expect = e-157, Method: Composition-based stats. Identities = 329/427 (77%), Positives = 364/427 (85%), Gaps = 4/427 (0%) Query: 19 LSVAAEDAIYRKVWLRIIPFLFICYVVSFLDRINIGFAQLQMKQDLGFSDAMYGLGAAVF 78 L+ EDA+YRKVWLRIIPFLFICYVVSFLDRINIGFAQLQMK DLGFSDAMYGLGAAVF Sbjct: 25 LAAHDEDALYRKVWLRIIPFLFICYVVSFLDRINIGFAQLQMKHDLGFSDAMYGLGAAVF 84 Query: 79 YVGYVLCEVPSNMLLARFGARRTFSRIMVLWGLASVGMMAVSDATHFYVLRFLLGVFEAG 138 YVGYVLCEVPSNMLLARFGARRTF+RIMVLWGLASV MMAVS T FY LRFLLGVFEAG Sbjct: 85 YVGYVLCEVPSNMLLARFGARRTFTRIMVLWGLASVAMMAVSTPTQFYTLRFLLGVFEAG 144 Query: 139 FFPGIVLYLSYWFPARRRAAVMAIFFAGVAVAGVLGGLVSGWIMRDMAGVMGLYGWQWMF 198 FFPGIVLYL+YWFPARRRAAVMAIFFAGVAVAGVLGGLVSGWIMRDMAGV+GL GW+WMF Sbjct: 145 FFPGIVLYLTYWFPARRRAAVMAIFFAGVAVAGVLGGLVSGWIMRDMAGVLGLQGWKWMF 204 Query: 199 AIEGAPAVVLGVLVIFLLADGPAQATWLTPEERSFLQNRQSQEALRTHHVSAHSLTALKQ 258 AIEGAPAV+LG++ L DGP QA+WLT ER+ L + +A H HSL AL+Q Sbjct: 205 AIEGAPAVLLGLVAARYLVDGPQQASWLTDGERAHLARDTAAQA----HAGGHSLHALRQ 260 Query: 259 ALSHRPTYLFAFIYFALTCGSLTLNFWMPLMIRDFGIKDVVQVSLYTVIPNAIGAIGLIL 318 LS+ YLFAFIYF+LTCGSL L+FWMPLMIRDFGI DV+ +SLY+V+PNA+GA+GLIL Sbjct: 261 VLSNPRVYLFAFIYFSLTCGSLALSFWMPLMIRDFGITDVMSISLYSVVPNAVGAVGLIL 320 Query: 319 IARRSDRRNERHGHFLVCTTGGALALAALTLHPSSFAVSLGLLSVAAVLIFAALPIFWSL 378 IAR SDR ERH HFL+CT GGALALAALTLH S AV L +LSVAAVLIFAALP+FWSL Sbjct: 321 IARHSDRTGERHRHFLLCTAGGALALAALTLHLPSLAVMLAILSVAAVLIFAALPVFWSL 380 Query: 379 PPGLLPSQTIAPGIAFISSVGITSGIVSPWLIGLVKTRTGNIDIALYILAGLLLLSGLTM 438 PP LP A GIAFISS+GITSGIVSPW+IG +KT+TG++D ALY+LA LLL SGL M Sbjct: 381 PPSYLPGAGTAAGIAFISSIGITSGIVSPWVIGQIKTQTGSLDNALYLLAALLLASGLAM 440 Query: 439 WTMIPRK 445 W +PR+ Sbjct: 441 WLGVPRQ 447