BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.0419.p (380 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003773699.1| poly(3-hydroxyalkanoate)synthetase [Herbaspi... 588 e-166 ref|YP_002799857.1| PHB de-polymerase [Azotobacter vinelandii DJ... 514 e-144 ref|ZP_02380638.1| PHB de-polymerase domain protein [Burkholderi... 507 e-141 ref|ZP_04943503.1| Alpha/beta hydrolase fold [Burkholderia cenoc... 504 e-140 ref|YP_623363.1| PHB de-polymerase-like [Burkholderia cenocepaci... 501 e-140 >ref|YP_003773699.1| poly(3-hydroxyalkanoate)synthetase [Herbaspirillum seropedicae SmR1] gb|ADJ61791.1| poly(3-hydroxyalkanoate)synthetase protein [Herbaspirillum seropedicae SmR1] Length = 376 Score = 588 bits (1515), Expect = e-166, Method: Composition-based stats. Identities = 292/358 (81%), Positives = 319/358 (89%), Gaps = 1/358 (0%) Query: 22 GCVPLFWPMMLATEVFRQGQEVVDKHLRFLEEETKLHSLHHPQMATANQVRLTLRTLSLR 81 G VPLFWPM A + F+QGQ+++DK LRFLEEE KLH++HHP+MAT ++ RL LRTL+LR Sbjct: 15 GSVPLFWPMAFAAQAFKQGQDMMDKQLRFLEEEAKLHAVHHPRMATPHRERLRLRTLTLR 74 Query: 82 EYGKPAAAHA-VPTLVVAPYAGHTSVIADYYRGQSLVETLMEHGVPHVFLTDWHSASEDM 140 EYG A HA VPTL+VAPYAGHTSVIADY +GQSLVETL+ +GV HV L DWHSAS DM Sbjct: 75 EYGSADAGHATVPTLIVAPYAGHTSVIADYDQGQSLVETLLGNGVDHVLLIDWHSASADM 134 Query: 141 KDLEIDNYLADLCVVIDELGGRANLVGLCQGGWISAMIAARFPHKVQRLVLAGSPIDAGA 200 KDLEIDNYLADLCVVIDELGGR LVGLCQGGWISAMIAARFP KV RLVLAGSPIDAGA Sbjct: 135 KDLEIDNYLADLCVVIDELGGRVRLVGLCQGGWISAMIAARFPEKVARLVLAGSPIDAGA 194 Query: 201 GEGPLKQMVERTPIRFYEDLVRTGGGLMRGSFMLQGWKNMHPDEHYFSEHVDLFQHIDDP 260 GEGPLKQMV+R P+RFYEDLVR+GGGLMRGSFMLQGWKNMHPDEHYF+EH++L QHIDDP Sbjct: 195 GEGPLKQMVQRLPLRFYEDLVRSGGGLMRGSFMLQGWKNMHPDEHYFNEHLELLQHIDDP 254 Query: 261 VYLAKREIFAAWYETPIDLPGRWYLQAIRQIFKDNLLARGQYVALGKMLDLRDVTCPLYL 320 YLAKRE FAAWYET +DLPGRWYLQAI IFKDNLLARG+YVALG+ LDLR +TCPLYL Sbjct: 255 AYLAKRETFAAWYETTVDLPGRWYLQAIGHIFKDNLLARGKYVALGQQLDLRQITCPLYL 314 Query: 321 LAGERDDITTPEQVLNAAQLVGTPAAHITQRVVPGGHIGLFMGGRTLAEAWPQIAQWL 378 LAGERDDITTPEQVLNAA LVGTPAA ITQ++VPGGHIGLFMG RTLAE WPQIA WL Sbjct: 315 LAGERDDITTPEQVLNAAALVGTPAAQITQKIVPGGHIGLFMGSRTLAEVWPQIATWL 372 >ref|YP_002799857.1| PHB de-polymerase [Azotobacter vinelandii DJ] gb|ACO78882.1| PHB de-polymerase [Azotobacter vinelandii DJ] Length = 372 Score = 514 bits (1325), Expect = e-144, Method: Composition-based stats. Identities = 239/367 (65%), Positives = 289/367 (78%), Gaps = 4/367 (1%) Query: 16 PAACGPGC--VPLFWPMMLATEVFRQGQEVVDKHLRFLEEETKLHSLHHPQMATANQVRL 73 P G C VP FWPM +A E+ +G E+ ++LRF++EE ++H P +AT NQVRL Sbjct: 7 PRGSGLSCASVPAFWPMTMAAELAEKGLELYARNLRFIQEEIRIHGELRPSLATPNQVRL 66 Query: 74 TLRTLSLREYGKPAAAHAVPTLVVAPYAGHTSVIADYYRGQSLVETLMEHGVPHVFLTDW 133 LRT+ LR+YG+P +PTLV APYAGHT++IADY+ GQSLVETL+ +G+ HV LTDW Sbjct: 67 GLRTMFLRDYGRPG--EGIPTLVDAPYAGHTAMIADYHAGQSLVETLLANGIGHVALTDW 124 Query: 134 HSASEDMKDLEIDNYLADLCVVIDELGGRANLVGLCQGGWISAMIAARFPHKVQRLVLAG 193 SA+EDMKD EIDNYL +L V ID+LGGR N+VGLCQGGW +AM+AARFP KV LVLAG Sbjct: 125 KSATEDMKDFEIDNYLGELVVAIDDLGGRVNIVGLCQGGWTAAMLAARFPDKVNSLVLAG 184 Query: 194 SPIDAGAGEGPLKQMVERTPIRFYEDLVRTGGGLMRGSFMLQGWKNMHPDEHYFSEHVDL 253 +PID AG+GP++ MV +PI FYEDLV GGGLM+G FMLQGWKNM P EHY EH+DL Sbjct: 185 APIDTDAGDGPIRGMVHESPISFYEDLVALGGGLMKGEFMLQGWKNMRPGEHYILEHIDL 244 Query: 254 FQHIDDPVYLAKREIFAAWYETPIDLPGRWYLQAIRQIFKDNLLARGQYVALGKMLDLRD 313 ++HIDDPVYL K+E F +WYE PIDLPGRWYLQ I Q+FK+N LARG++ LG+ LDLRD Sbjct: 245 YEHIDDPVYLRKKETFESWYENPIDLPGRWYLQVIGQLFKENRLARGEFAGLGRRLDLRD 304 Query: 314 VTCPLYLLAGERDDITTPEQVLNAAQLVGTPAAHITQRVVPGGHIGLFMGGRTLAEAWPQ 373 +TCPLYLLAGE DDIT+PEQV NAA LVGTP I +++VPGGHIGLFMG RTL E WP Sbjct: 305 ITCPLYLLAGEGDDITSPEQVFNAANLVGTPQERIVRQLVPGGHIGLFMGARTLTERWPG 364 Query: 374 IAQWLAA 380 IA+W+AA Sbjct: 365 IARWIAA 371 >ref|ZP_02380638.1| PHB de-polymerase domain protein [Burkholderia ubonensis Bu] Length = 373 Score = 507 bits (1305), Expect = e-141, Method: Composition-based stats. Identities = 240/369 (65%), Positives = 293/369 (79%), Gaps = 7/369 (1%) Query: 12 PAPEPAACGPGCVPLFWPMMLATEVFRQGQEVVDKHLRFLEEETKLHSLHHPQMATANQV 71 P P PA +P+FWPM AT +F+ G E+ ++LRFL EE KLH HP +A+AN+ Sbjct: 10 PFPIPA------LPIFWPMAAATAMFKAGTELTARNLRFLAEEEKLHFEMHPALASANRP 63 Query: 72 RLTLRTLSLREYGKPAAAHAVPTLVVAPYAGHTSVIADYYRGQSLVETLMEHGVPHVFLT 131 L LRT+ R+Y AA +PT+V APYAGH+++IADY GQSL++TL EHGV ++LT Sbjct: 64 LLELRTMIFRDYSD-GAARGLPTIVDAPYAGHSAMIADYQPGQSLMQTLREHGVSRLYLT 122 Query: 132 DWHSASEDMKDLEIDNYLADLCVVIDELGGRANLVGLCQGGWISAMIAARFPHKVQRLVL 191 DW SA+EDMKDLEID YLA+L V +DELGGR N VGLCQGGW++AM AARFPHKV LVL Sbjct: 123 DWRSATEDMKDLEIDQYLAELNVCVDELGGRVNFVGLCQGGWMAAMYAARFPHKVASLVL 182 Query: 192 AGSPIDAGAGEGPLKQMVERTPIRFYEDLVRTGGGLMRGSFMLQGWKNMHPDEHYFSEHV 251 AGSPID AG GP++QMV P FYE+LV GGGLMRG FML+GWKNMHPD+HY +EHV Sbjct: 183 AGSPIDTDAGNGPIRQMVHTYPTSFYEELVELGGGLMRGRFMLRGWKNMHPDQHYLAEHV 242 Query: 252 DLFQHIDDPVYLAKREIFAAWYETPIDLPGRWYLQAIRQIFKDNLLARGQYVALGKMLDL 311 DL++H+DDP YL K+E FA+WYE+PIDLPGRWYLQAI Q+FK+N LA+G +VALG+ L+L Sbjct: 243 DLYEHLDDPDYLRKQEAFASWYESPIDLPGRWYLQAIVQLFKENRLAKGAFVALGRTLNL 302 Query: 312 RDVTCPLYLLAGERDDITTPEQVLNAAQLVGTPAAHITQRVVPGGHIGLFMGGRTLAEAW 371 +DVTCP+YLLAGE DDITTPEQV +A + +GTPAA +T R+VPGGHIGLFMG RTL +AW Sbjct: 303 KDVTCPVYLLAGEADDITTPEQVFDAVKYLGTPAAQVTSRLVPGGHIGLFMGSRTLKDAW 362 Query: 372 PQIAQWLAA 380 P IA W+AA Sbjct: 363 PDIAAWIAA 371 >ref|ZP_04943503.1| Alpha/beta hydrolase fold [Burkholderia cenocepacia PC184] gb|EAY66674.1| Alpha/beta hydrolase fold [Burkholderia cenocepacia PC184] Length = 435 Score = 504 bits (1297), Expect = e-140, Method: Composition-based stats. Identities = 245/367 (66%), Positives = 292/367 (79%), Gaps = 2/367 (0%) Query: 14 PEPAACGPGCVPLFWPMMLATEVFRQGQEVVDKHLRFLEEETKLHSLHHPQMATANQVRL 73 P PA P +P+FWPM AT +F G E+ ++LRFL EE KLH HP +A+ N+ L Sbjct: 69 PSPAVSAPA-LPIFWPMAAATAMFAAGAEIAARNLRFLAEEEKLHFEAHPALASPNRSLL 127 Query: 74 TLRTLSLREYGKPAAAHAVPTLVVAPYAGHTSVIADYYRGQSLVETLMEHGVPHVFLTDW 133 LRT++ R+Y A AH +PT+V APYAGH+++IADY GQSL++TL EHGV ++LTDW Sbjct: 128 ELRTMTFRDYSA-APAHGLPTIVDAPYAGHSAMIADYQPGQSLMQTLREHGVTRLYLTDW 186 Query: 134 HSASEDMKDLEIDNYLADLCVVIDELGGRANLVGLCQGGWISAMIAARFPHKVQRLVLAG 193 SA+EDMKDLEID YLA+L V +DELGGR NLVGLCQGGW+SAM AARFPHKV LVLAG Sbjct: 187 RSATEDMKDLEIDQYLAELNVCVDELGGRVNLVGLCQGGWMSAMYAARFPHKVASLVLAG 246 Query: 194 SPIDAGAGEGPLKQMVERTPIRFYEDLVRTGGGLMRGSFMLQGWKNMHPDEHYFSEHVDL 253 SPID AG+GP+KQMV P FYE+LV GGGLMRG FML+GWKNMHPD+HY +EHVDL Sbjct: 247 SPIDTDAGDGPIKQMVHTYPTSFYEELVAMGGGLMRGRFMLRGWKNMHPDQHYLAEHVDL 306 Query: 254 FQHIDDPVYLAKREIFAAWYETPIDLPGRWYLQAIRQIFKDNLLARGQYVALGKMLDLRD 313 ++H+DDP YL KRE+FA WYE+PIDLPGRWYLQAI QIFK+N LA+G +VALG+ LDL+D Sbjct: 307 YEHLDDPEYLRKREVFANWYESPIDLPGRWYLQAIVQIFKENRLAKGTFVALGRTLDLKD 366 Query: 314 VTCPLYLLAGERDDITTPEQVLNAAQLVGTPAAHITQRVVPGGHIGLFMGGRTLAEAWPQ 373 V CP+YLLAGE DDITTPEQV A L+GTPA+ I R+VPGGHIGLFMG RTLA WP Sbjct: 367 VACPIYLLAGEADDITTPEQVFGARDLLGTPASRIESRLVPGGHIGLFMGTRTLATVWPD 426 Query: 374 IAQWLAA 380 IA+W+AA Sbjct: 427 IARWIAA 433 >ref|YP_623363.1| PHB de-polymerase-like [Burkholderia cenocepacia AU 1054] ref|YP_838497.1| PHB de-polymerase domain-containing protein [Burkholderia cenocepacia HI2424] gb|ABF78390.1| PHB de-polymerase-like protein [Burkholderia cenocepacia AU 1054] gb|ABK11604.1| PHB de-polymerase domain protein [Burkholderia cenocepacia HI2424] Length = 373 Score = 501 bits (1290), Expect = e-140, Method: Composition-based stats. Identities = 242/367 (65%), Positives = 292/367 (79%), Gaps = 2/367 (0%) Query: 14 PEPAACGPGCVPLFWPMMLATEVFRQGQEVVDKHLRFLEEETKLHSLHHPQMATANQVRL 73 P PA P +P+FWPM AT +F G E+ ++LRFL EE KLH HP +A+ N+ L Sbjct: 7 PSPAVSAPA-LPIFWPMAAATAMFAAGAEIAARNLRFLAEEEKLHFEAHPALASPNRPLL 65 Query: 74 TLRTLSLREYGKPAAAHAVPTLVVAPYAGHTSVIADYYRGQSLVETLMEHGVPHVFLTDW 133 LRT++ R+Y A AH +PT+V APYAGH+++IADY GQSL++TL EHGV V+LTDW Sbjct: 66 ELRTMTFRDYSA-APAHGLPTIVDAPYAGHSAMIADYQPGQSLMQTLREHGVTRVYLTDW 124 Query: 134 HSASEDMKDLEIDNYLADLCVVIDELGGRANLVGLCQGGWISAMIAARFPHKVQRLVLAG 193 SA+EDMKDLEID YLA+L V +DELGGR NLVGLCQGGW+SAM AARFPHKV LVLAG Sbjct: 125 RSATEDMKDLEIDQYLAELNVCVDELGGRVNLVGLCQGGWMSAMYAARFPHKVASLVLAG 184 Query: 194 SPIDAGAGEGPLKQMVERTPIRFYEDLVRTGGGLMRGSFMLQGWKNMHPDEHYFSEHVDL 253 SPID AG+GP+K+MV P FY++LV GGGLMRG FML+GWKNMHPD+HY +EH+DL Sbjct: 185 SPIDTDAGDGPIKRMVHTYPTAFYDELVAMGGGLMRGRFMLRGWKNMHPDQHYLAEHIDL 244 Query: 254 FQHIDDPVYLAKREIFAAWYETPIDLPGRWYLQAIRQIFKDNLLARGQYVALGKMLDLRD 313 ++H+DDP YL KRE+FA WYE+PIDLPGRWYLQAI QIFK+N LA+G +VALG+ LDL+D Sbjct: 245 YEHLDDPEYLRKREVFANWYESPIDLPGRWYLQAIVQIFKENRLAKGTFVALGRTLDLKD 304 Query: 314 VTCPLYLLAGERDDITTPEQVLNAAQLVGTPAAHITQRVVPGGHIGLFMGGRTLAEAWPQ 373 V CP+YLLAGE DDITTPEQV A L+GTPA+ I R+VPGGHIG+FMG RTLA WP Sbjct: 305 VACPVYLLAGEADDITTPEQVFGARDLLGTPASRIESRLVPGGHIGMFMGTRTLATVWPD 364 Query: 374 IAQWLAA 380 IA+W+AA Sbjct: 365 IARWIAA 371