BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.0257.p (411 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003775043.1| poly[D(-)-3-hydroxyalkanoate] depolymerase [... 815 0.0 ref|YP_004753167.1| poly[D(-)-3-Hydroxybutyrate] depolymerase [C... 701 0.0 ref|YP_001099121.1| poly[D(-)-3-hydroxybutyrate] depolymerase [H... 690 0.0 ref|YP_001352408.1| Poly-beta-hydroxyalkanoate depolymerase [Jan... 684 0.0 gb|ACN58951.1| poly[D(--3-Hydroxybutyrate] depolymerase [uncultu... 666 0.0 >ref|YP_003775043.1| poly[D(-)-3-hydroxyalkanoate] depolymerase [Herbaspirillum seropedicae SmR1] gb|ADJ63135.1| poly[D(-)-3-hydroxyalkanoate] depolymerase protein [Herbaspirillum seropedicae SmR1] Length = 411 Score = 815 bits (2104), Expect = 0.0, Method: Composition-based stats. Identities = 397/411 (96%), Positives = 402/411 (97%) Query: 1 MLYQLHELNRAFLNPMMQWAETSAKLFSDPVSPLAHTPFSQRIAAGYELLYRLSKEYEKP 60 MLYQLHELNRAFLNPMMQWAETSAKLFSDPVSPLAHTPFSQRIAAGYELLYRLSKEYEKP Sbjct: 1 MLYQLHELNRAFLNPMMQWAETSAKLFSDPVSPLAHTPFSQRIAAGYELLYRLSKEYEKP 60 Query: 61 LFNIGEVPVDGKPVGITEEVVEEKPFCRLIHFKKDLSARQSAALKQPTVLVVAPLSGHHS 120 FNI VPVDGK VGI EEVVEEKPFCRLIHF+KDLSARQ+ ALKQPTVLVVAPLSGHHS Sbjct: 61 QFNIDAVPVDGKSVGIVEEVVEEKPFCRLIHFRKDLSARQATALKQPTVLVVAPLSGHHS 120 Query: 121 TLLRETVRALLQEHDVFITDWTDARMVPVEAGEFHLHDYVYYVQDFIRTLGPDVHVISVC 180 TLLRETVRALLQEHDV+ITDWTDARMVPVE G FHLHDYVYYVQDFIRTLGPDVHVISVC Sbjct: 121 TLLRETVRALLQEHDVYITDWTDARMVPVEQGAFHLHDYVYYVQDFIRTLGPDVHVISVC 180 Query: 181 QPTVPVLAAISLMASANDPKLPKSMTMMGGPIDARKSPTAVNDLATEKPFSWFENTVIYS 240 QPTVPVLAAISLMASANDP LPKSMTMMGGPIDARKSPTAVNDLATEKP+SWFENTVIYS Sbjct: 181 QPTVPVLAAISLMASANDPMLPKSMTMMGGPIDARKSPTAVNDLATEKPYSWFENTVIYS 240 Query: 241 VPANYPGFGRKVYPGFLQHAGFVAMNPRRHAQSHWDFYMHLRDGDDASAEEHRKFYDEYN 300 VPANYPGFGRKVYPGFLQHAGFVAMNPRRHAQSHWDFYMHLRDGDDASAEEHRKFYDEYN Sbjct: 241 VPANYPGFGRKVYPGFLQHAGFVAMNPRRHAQSHWDFYMHLRDGDDASAEEHRKFYDEYN 300 Query: 301 AVLDMPAEFYLETIKVVFQDFNLARGTWEIEGKLVRPQDIKSVALFTIEGELDDISGSGQ 360 AVLDMPAEFYLETIKVVFQDFNLARGTWEIEG+LVRPQDIKSVALFTIEGELDDISGSGQ Sbjct: 301 AVLDMPAEFYLETIKVVFQDFNLARGTWEIEGQLVRPQDIKSVALFTIEGELDDISGSGQ 360 Query: 361 TQAAQELCSSIPKARKQHFTVPKAGHYGIFSGRRWREIVCPKIGEFIRANA 411 TQAAQELCSSIPKARKQHFTVPKAGHYGIFSGRRWREIVCPKIGEFIRANA Sbjct: 361 TQAAQELCSSIPKARKQHFTVPKAGHYGIFSGRRWREIVCPKIGEFIRANA 411 >ref|YP_004753167.1| poly[D(-)-3-Hydroxybutyrate] depolymerase [Collimonas fungivorans Ter331] gb|AEK62344.1| poly[D(-)-3-Hydroxybutyrate] depolymerase (PHB depolymerase) [Collimonas fungivorans Ter331] Length = 411 Score = 701 bits (1810), Expect = 0.0, Method: Composition-based stats. Identities = 323/411 (78%), Positives = 363/411 (88%) Query: 1 MLYQLHELNRAFLNPMMQWAETSAKLFSDPVSPLAHTPFSQRIAAGYELLYRLSKEYEKP 60 MLYQ HE NR+ L+P++QWA+ ++LF+DP+SPL+HTPF+QRIAAGYEL+YRL K+YEKP Sbjct: 1 MLYQFHEFNRSLLHPLVQWADAGSRLFTDPISPLSHTPFAQRIAAGYELMYRLGKQYEKP 60 Query: 61 LFNIGEVPVDGKPVGITEEVVEEKPFCRLIHFKKDLSARQSAALKQPTVLVVAPLSGHHS 120 F I V+ K V I EEV KPFCRL+HF+KDLSA++ ALKQPTVL+VAPLSGHHS Sbjct: 61 EFEIDSTLVNDKTVAIIEEVATTKPFCRLLHFRKDLSAKELGALKQPTVLLVAPLSGHHS 120 Query: 121 TLLRETVRALLQEHDVFITDWTDARMVPVEAGEFHLHDYVYYVQDFIRTLGPDVHVISVC 180 TLLR+TVR LL EHDV+ITDWTDARMVP G FHLHDY+YY+QDFIR LGPD+HV+SVC Sbjct: 121 TLLRDTVRGLLPEHDVYITDWTDARMVPASDGAFHLHDYIYYIQDFIRQLGPDLHVVSVC 180 Query: 181 QPTVPVLAAISLMASANDPKLPKSMTMMGGPIDARKSPTAVNDLATEKPFSWFENTVIYS 240 QPTVPVLAAISLMASA D LPKSMTMMGGPID R+SPT VN+LATEKPFSWFENTVIYS Sbjct: 181 QPTVPVLAAISLMASAKDSHLPKSMTMMGGPIDPRESPTDVNNLATEKPFSWFENTVIYS 240 Query: 241 VPANYPGFGRKVYPGFLQHAGFVAMNPRRHAQSHWDFYMHLRDGDDASAEEHRKFYDEYN 300 VP+NYPGFGRKVYPGFLQHAGFVAMNP RHAQSHWDFYMHLR+GD+ SA+ HRKFY+EYN Sbjct: 241 VPSNYPGFGRKVYPGFLQHAGFVAMNPSRHAQSHWDFYMHLREGDNESADSHRKFYNEYN 300 Query: 301 AVLDMPAEFYLETIKVVFQDFNLARGTWEIEGKLVRPQDIKSVALFTIEGELDDISGSGQ 360 AVLDMPAE+YLETIK VFQDF+L GTWE+EGKLVRPQDIKSVALFTIEGELDDISG GQ Sbjct: 301 AVLDMPAEYYLETIKTVFQDFSLPHGTWEVEGKLVRPQDIKSVALFTIEGELDDISGPGQ 360 Query: 361 TQAAQELCSSIPKARKQHFTVPKAGHYGIFSGRRWREIVCPKIGEFIRANA 411 TQAA LCSSIPK++KQHFT K GHYGIFSGRRWRE++CPKI FI+ NA Sbjct: 361 TQAAHGLCSSIPKSKKQHFTAEKCGHYGIFSGRRWRELICPKITAFIKDNA 411 >ref|YP_001099121.1| poly[D(-)-3-hydroxybutyrate] depolymerase [Herminiimonas arsenicoxydans] emb|CAL60994.1| poly[D(-)-3-Hydroxybutyrate] depolymerase (PHB depolymerase) [Herminiimonas arsenicoxydans] Length = 411 Score = 690 bits (1780), Expect = 0.0, Method: Composition-based stats. Identities = 322/407 (79%), Positives = 362/407 (88%) Query: 1 MLYQLHELNRAFLNPMMQWAETSAKLFSDPVSPLAHTPFSQRIAAGYELLYRLSKEYEKP 60 MLYQLHE NR+ LNP+M WAE S++LF+DP SPLA+TP++QRIAAGYELLYRL K+YEKP Sbjct: 1 MLYQLHEFNRSLLNPLMHWAEASSRLFTDPASPLAYTPYAQRIAAGYELLYRLGKQYEKP 60 Query: 61 LFNIGEVPVDGKPVGITEEVVEEKPFCRLIHFKKDLSARQSAALKQPTVLVVAPLSGHHS 120 FNI V V+GK V I EE E+K FC LIHF+K LSA++ AALKQP VL+VAPLSGHHS Sbjct: 61 NFNIESVLVNGKTVAIVEEETEKKSFCTLIHFRKKLSAKEFAALKQPVVLLVAPLSGHHS 120 Query: 121 TLLRETVRALLQEHDVFITDWTDARMVPVEAGEFHLHDYVYYVQDFIRTLGPDVHVISVC 180 TLLR+TVR+LLQ+HDV+ITDWTDARMVP+ G FHLHDYVYY+QDFIR LGPD+HVISVC Sbjct: 121 TLLRDTVRSLLQDHDVYITDWTDARMVPLAEGPFHLHDYVYYIQDFIRRLGPDLHVISVC 180 Query: 181 QPTVPVLAAISLMASANDPKLPKSMTMMGGPIDARKSPTAVNDLATEKPFSWFENTVIYS 240 QPTVPVLAAISLMAS DP+LPK+MTMMGGPIDARKSPT VNDLA EKP +WFEN VIYS Sbjct: 181 QPTVPVLAAISLMASERDPRLPKTMTMMGGPIDARKSPTQVNDLAVEKPHAWFENQVIYS 240 Query: 241 VPANYPGFGRKVYPGFLQHAGFVAMNPRRHAQSHWDFYMHLRDGDDASAEEHRKFYDEYN 300 VP +YPGFGRKVYPGFLQHAGF+AMNP RHAQSHWDFYMHLR+GD+ SAEEHRKFYDEYN Sbjct: 241 VPPSYPGFGRKVYPGFLQHAGFIAMNPGRHAQSHWDFYMHLREGDNESAEEHRKFYDEYN 300 Query: 301 AVLDMPAEFYLETIKVVFQDFNLARGTWEIEGKLVRPQDIKSVALFTIEGELDDISGSGQ 360 AVLDMPAE+YLETIK VFQ+ L GTWEIEGKLVRPQDIKSVALFTIEGE DDISG+GQ Sbjct: 301 AVLDMPAEYYLETIKTVFQEHRLPLGTWEIEGKLVRPQDIKSVALFTIEGEKDDISGAGQ 360 Query: 361 TQAAQELCSSIPKARKQHFTVPKAGHYGIFSGRRWREIVCPKIGEFI 407 T AAQ+LCS++P++ KQ FT P+ GHYGIFSGR+WREI+ PKIGEFI Sbjct: 361 THAAQDLCSALPQSDKQQFTAPQCGHYGIFSGRKWREIIAPKIGEFI 407 >ref|YP_001352408.1| Poly-beta-hydroxyalkanoate depolymerase [Janthinobacterium sp. Marseille] gb|ABR89657.1| Poly-beta-hydroxyalkanoate depolymerase [Janthinobacterium sp. Marseille] Length = 411 Score = 684 bits (1766), Expect = 0.0, Method: Composition-based stats. Identities = 319/407 (78%), Positives = 360/407 (88%) Query: 1 MLYQLHELNRAFLNPMMQWAETSAKLFSDPVSPLAHTPFSQRIAAGYELLYRLSKEYEKP 60 MLYQLHELNR+ LNP++QWAE S+KLFSDPVSP A+TPF+Q IAAGYEL+YRL K+Y KP Sbjct: 1 MLYQLHELNRSLLNPLIQWAEASSKLFSDPVSPFAYTPFAQPIAAGYELMYRLGKDYAKP 60 Query: 61 LFNIGEVPVDGKPVGITEEVVEEKPFCRLIHFKKDLSARQSAALKQPTVLVVAPLSGHHS 120 F+I V + GK V I EEV EEK FC L+HFKKD+SA+ AALKQPT+L+VAPLSGHH+ Sbjct: 61 HFDISSVNIKGKDVKIIEEVAEEKSFCTLLHFKKDMSAKAYAALKQPTILLVAPLSGHHA 120 Query: 121 TLLRETVRALLQEHDVFITDWTDARMVPVEAGEFHLHDYVYYVQDFIRTLGPDVHVISVC 180 TLLR+TVR+LL EHDV+ITDW DARMVP+ G FHLHDY+YY+QDFIR LGP++HVISVC Sbjct: 121 TLLRDTVRSLLVEHDVYITDWRDARMVPLTEGPFHLHDYIYYIQDFIRQLGPNLHVISVC 180 Query: 181 QPTVPVLAAISLMASANDPKLPKSMTMMGGPIDARKSPTAVNDLATEKPFSWFENTVIYS 240 QPTVPVLAAISLMAS DP LPKSMTMMGGPID RKSPT VN+LATEKP+SWFEN VI+S Sbjct: 181 QPTVPVLAAISLMASERDPHLPKSMTMMGGPIDPRKSPTQVNNLATEKPYSWFENQVIHS 240 Query: 241 VPANYPGFGRKVYPGFLQHAGFVAMNPRRHAQSHWDFYMHLRDGDDASAEEHRKFYDEYN 300 VPA+YPGFGRKVYPGFLQHAGF+AMNP RHAQSHWDFYMHLR+GD+ SAEEHRKFYDEYN Sbjct: 241 VPASYPGFGRKVYPGFLQHAGFIAMNPGRHAQSHWDFYMHLREGDNESAEEHRKFYDEYN 300 Query: 301 AVLDMPAEFYLETIKVVFQDFNLARGTWEIEGKLVRPQDIKSVALFTIEGELDDISGSGQ 360 AVLDMPAE+YLETIK VFQ+ L GTWE+EGKLV+PQDIKSVALFTIEGE DDISG GQ Sbjct: 301 AVLDMPAEYYLETIKTVFQEHRLPLGTWEVEGKLVKPQDIKSVALFTIEGERDDISGLGQ 360 Query: 361 TQAAQELCSSIPKARKQHFTVPKAGHYGIFSGRRWREIVCPKIGEFI 407 T AAQ+LCSSI K+ KQ FT P+ GHYGIFSGR+WREI+CPKI +FI Sbjct: 361 THAAQDLCSSIAKSNKQQFTAPQCGHYGIFSGRKWREIICPKITDFI 407 >gb|ACN58951.1| poly[D(--3-Hydroxybutyrate] depolymerase [uncultured bacterium BLR10] Length = 411 Score = 666 bits (1718), Expect = 0.0, Method: Composition-based stats. Identities = 314/408 (76%), Positives = 356/408 (87%) Query: 1 MLYQLHELNRAFLNPMMQWAETSAKLFSDPVSPLAHTPFSQRIAAGYELLYRLSKEYEKP 60 MLYQLHE+ R+ L P+MQWA+ S+KLF++PVSPLAHTPFSQRIAAGYEL+YRL K+YEKP Sbjct: 1 MLYQLHEMQRSLLTPLMQWADASSKLFTNPVSPLAHTPFSQRIAAGYELMYRLGKDYEKP 60 Query: 61 LFNIGEVPVDGKPVGITEEVVEEKPFCRLIHFKKDLSARQSAALKQPTVLVVAPLSGHHS 120 F I + V+G+ VGI E+VV KPFCRLIHFKKDL ++ ALKQPTVL+VAPLSGHHS Sbjct: 61 AFEIDDALVNGEAVGIIEQVVVNKPFCRLIHFKKDLDDKKIKALKQPTVLLVAPLSGHHS 120 Query: 121 TLLRETVRALLQEHDVFITDWTDARMVPVEAGEFHLHDYVYYVQDFIRTLGPDVHVISVC 180 TLLR+TVR LL +HDV+ITDWTDARMVP+ G FHL DY++YVQDFIR LGPDVHVISVC Sbjct: 121 TLLRDTVRGLLVDHDVYITDWTDARMVPLSEGAFHLDDYIFYVQDFIRQLGPDVHVISVC 180 Query: 181 QPTVPVLAAISLMASANDPKLPKSMTMMGGPIDARKSPTAVNDLATEKPFSWFENTVIYS 240 QPTVPVLAAISLMAS NDPKLPK+MTMMGGPID R+SPT VN+LATEK FSWFENTVIY+ Sbjct: 181 QPTVPVLAAISLMASENDPKLPKTMTMMGGPIDPRRSPTQVNNLATEKKFSWFENTVIYA 240 Query: 241 VPANYPGFGRKVYPGFLQHAGFVAMNPRRHAQSHWDFYMHLRDGDDASAEEHRKFYDEYN 300 VP NYPGFGRKVYPGFLQHAGF+AMNP RHAQSH +FYMHL +GDD A+ HRKFYDEYN Sbjct: 241 VPPNYPGFGRKVYPGFLQHAGFIAMNPGRHAQSHREFYMHLVEGDDEPADSHRKFYDEYN 300 Query: 301 AVLDMPAEFYLETIKVVFQDFNLARGTWEIEGKLVRPQDIKSVALFTIEGELDDISGSGQ 360 AVLDMPAE+YL+TIK VFQDF+L +G W + GKLVRPQDI +VALFT+EGELDDISG+GQ Sbjct: 301 AVLDMPAEYYLDTIKTVFQDFSLPKGEWVVGGKLVRPQDITTVALFTVEGELDDISGAGQ 360 Query: 361 TQAAQELCSSIPKARKQHFTVPKAGHYGIFSGRRWREIVCPKIGEFIR 408 TQAA +LCS IP +Q F P AGHYGIFSGRRWRE+VCPKI EFI+ Sbjct: 361 TQAAHDLCSGIPADMQQDFVAPGAGHYGIFSGRRWREMVCPKITEFIK 408