BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.0231.p (546 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003775020.1| medium-chain-fatty-acid CoA ligase [Herbaspi... 1009 0.0 gb|EGW62287.1| AMP-dependent synthetase and ligase [Dechlorosoma... 772 0.0 ref|YP_004847238.1| AMP-dependent synthetase and ligase [Pseudog... 705 0.0 ref|YP_284122.1| acyl-CoA synthetase [Dechloromonas aromatica RC... 686 0.0 gb|EGW62605.1| AMP-dependent synthetase and ligase [Dechlorosoma... 684 0.0 >ref|YP_003775020.1| medium-chain-fatty-acid CoA ligase [Herbaspirillum seropedicae SmR1] gb|ADJ63112.1| medium-chain-fatty-acid CoA ligase protein [Herbaspirillum seropedicae SmR1] Length = 545 Score = 1009 bits (2610), Expect = 0.0, Method: Composition-based stats. Identities = 498/545 (91%), Positives = 517/545 (94%) Query: 1 MPQTIEQTPSAYGYPLLIKQLLHSALATAPEQEIIYGARRLTYAGFYERVQRLANVLKEM 60 MPQ I QTPSAYGYPLLIKQLLHSALATA QEIIYG RRL+YAGFYERVQRLAN LKEM Sbjct: 1 MPQAIHQTPSAYGYPLLIKQLLHSALATASGQEIIYGQRRLSYAGFYERVQRLANALKEM 60 Query: 61 GVQAGNTVAVMDWDSNRYLECFFAVPMMGCVLQTVNVRLSPEQIAYTLNHAQADVLLVNS 120 G+Q GNTVAVMDWDSNRYLECFFAVPMMGCVLQTVNVRLSPEQIAYTLNHAQADVLLVNS Sbjct: 61 GLQPGNTVAVMDWDSNRYLECFFAVPMMGCVLQTVNVRLSPEQIAYTLNHAQADVLLVNS 120 Query: 121 DFMPVLKQIRGELNTLTRCVLLTDDGSPCPQGPGFAGEYEALLAQASAQYDFPDFDENAR 180 DF+P+LKQIRGEL TLTRCV LTDD +PCP+GPGFAGEYE LLAQA DFPDFDENAR Sbjct: 121 DFLPLLKQIRGELTTLTRCVWLTDDATPCPRGPGFAGEYEELLAQAEPACDFPDFDENAR 180 Query: 181 ATTFYTTGTTGLPKGVYFSHRQLVLHTLGTTAGLALAPQQGRLHRDDVYMPLTPMFHVHA 240 ATTFYTTGTTGLPKGVYFSHRQLVLHTL TTAGLALAPQQGR HRDDVYMPLTPMFHVHA Sbjct: 181 ATTFYTTGTTGLPKGVYFSHRQLVLHTLATTAGLALAPQQGRFHRDDVYMPLTPMFHVHA 240 Query: 241 WGLPYVATMAGVKQVYPGRYVPDTICKLIREEKVTFSHCVPTILQMVLTSEQAREVDLSG 300 WGLPYVATMAGVKQVYPGRYVPDTIC+LIREEKVTFSHCVPTILQMVLTSEQARE DLSG Sbjct: 241 WGLPYVATMAGVKQVYPGRYVPDTICRLIREEKVTFSHCVPTILQMVLTSEQAREADLSG 300 Query: 301 WKMIIGGSAMTGSLARMARDRGIDVFTGYGMSETCPILTLAQVPTEDLGSEREAVLRVKT 360 WKMIIGGSAMTGSLA+MARDRGIDVFTGYGMSETCP+LTLAQVPTE+LG EREA +RVKT Sbjct: 301 WKMIIGGSAMTGSLAKMARDRGIDVFTGYGMSETCPVLTLAQVPTEELGGEREAAIRVKT 360 Query: 361 GRPVPLVALRTVDGDMNDCPRDGKSTGEVVVRAPWLTQGYLHQAEASEELWRGGWLHTQD 420 GRP+PLV LRTVDG+M DCPRDG +TGEVVVRAPWLTQGYLHQAEASEELWRGGWLHTQD Sbjct: 361 GRPLPLVDLRTVDGEMQDCPRDGSTTGEVVVRAPWLTQGYLHQAEASEELWRGGWLHTQD 420 Query: 421 IGHLDAEGYLQVTDRIKDVIKTGGEWVSSLQIEDIIARHPAVAETAVIGVQDGKWGERPV 480 IGHLDAEGYLQVTDRIKDVIKTGGEWVSSLQIEDIIARHPAVAETAVIG+QDGKWGERPV Sbjct: 421 IGHLDAEGYLQVTDRIKDVIKTGGEWVSSLQIEDIIARHPAVAETAVIGIQDGKWGERPV 480 Query: 481 ALVVLKKDVDAVSEEDIRQHVMAAAEAGTISRYGVPDRVRFVPELARTSVGKLNKRVMRE 540 ALVVLKKD AVSE+DIR+HV AAAEAG ISRYGVPDRVRFV ELARTSVGKLNKRVMRE Sbjct: 481 ALVVLKKDAAAVSEDDIREHVRAAAEAGAISRYGVPDRVRFVAELARTSVGKLNKRVMRE 540 Query: 541 QYPAA 545 QYP++ Sbjct: 541 QYPSS 545 >gb|EGW62287.1| AMP-dependent synthetase and ligase [Dechlorosoma suillum PS] Length = 547 Score = 772 bits (1994), Expect = 0.0, Method: Composition-based stats. Identities = 391/543 (72%), Positives = 444/543 (81%), Gaps = 6/543 (1%) Query: 3 QTIEQTPSAYGYPLLIKQLLHSALATAPEQEIIYGAR-RLTYAGFYERVQRLANVLKEMG 61 Q I+ TPSAY YPLLIKQLLH+ LATAP+QEI+YG + R TY R+ R + L +G Sbjct: 4 QPIQVTPSAYTYPLLIKQLLHTPLATAPQQEIVYGDKVRHTYRDLALRINRQGHALSRLG 63 Query: 62 VQAGNTVAVMDWDSNRYLECFFAVPMMGCVLQTVNVRLSPEQIAYTLNHAQADVLLVNSD 121 VQ G+TVAVMDWDS+RYLECFF VPM+G VLQTVNVRLSP+QI YTLNHAQA VLLVN + Sbjct: 64 VQGGDTVAVMDWDSHRYLECFFGVPMLGAVLQTVNVRLSPDQILYTLNHAQASVLLVNRE 123 Query: 122 FMPVLKQIRGELNTLTRCVLLTDDGSPCPQGPGFAGEYEALLAQASAQYDFPDFDENARA 181 F+PVL QIRGEL T+ R VLL D+G P+G FAGEYEALL YDFPDFDEN+RA Sbjct: 124 FLPVLAQIRGELATVKRFVLLDDEGGDLPEG--FAGEYEALLIDCPDHYDFPDFDENSRA 181 Query: 182 TTFYTTGTTGLPKGVYFSHRQLVLHTLGTTAGLALAPQQGRLHRDDVYMPLTPMFHVHAW 241 TTFYTTGTTGLPKGVYFSHRQLVLHTL + LALAPQQG HRDD+YMPLTPMFHVHAW Sbjct: 182 TTFYTTGTTGLPKGVYFSHRQLVLHTLAVCSALALAPQQGGFHRDDIYMPLTPMFHVHAW 241 Query: 242 GLPYVATMAGVKQVYPGRYVPDTICKLIREEKVTFSHCVPTILQMVLTSEQAREVDLSGW 301 G+PY+AT+AGVKQVYPGRY P+ I KLI +E+VTFSHCVPTIL M+L + ++VDLS W Sbjct: 242 GMPYIATLAGVKQVYPGRYQPEAILKLIEKEQVTFSHCVPTILHMLLLTPYGKQVDLSRW 301 Query: 302 KMIIGGSAMTGSLARMARDRGIDVFTGYGMSETCPILTLAQVPTEDLGSEREA--VLRVK 359 K+IIGGSA+T LA+ A +RGIDVFTGYGMSETCPILTLAQ+ L ++ +A +RVK Sbjct: 302 KVIIGGSALTTGLAKAALERGIDVFTGYGMSETCPILTLAQMEDPALLADADANVAVRVK 361 Query: 360 TGRPVPLVALRTVDGDMNDCPRDGKSTGEVVVRAPWLTQGYLHQAEASEELWRGGWLHTQ 419 TGRPVPLVALRTVD M DCPRDGKS GEVVVRAPWLTQGYL+ EASE LW GGWLHTQ Sbjct: 362 TGRPVPLVALRTVDAQMGDCPRDGKSAGEVVVRAPWLTQGYLYNPEASEHLWDGGWLHTQ 421 Query: 420 DIGHLDAEGYLQVTDRIKDVIKTGGEWVSSLQIEDIIARHPAVAETAVIGVQDGKWGERP 479 DIGH+D +GYLQVTDRIKDVIKTGGEWVSSL++ED+I RHP V+E AVIGV D KWGERP Sbjct: 422 DIGHIDPQGYLQVTDRIKDVIKTGGEWVSSLELEDLIGRHPGVSEVAVIGVPDEKWGERP 481 Query: 480 VALVVLKKD-VDAVSEEDIRQHVMAAAEAGTISRYGVPDRVRFVPELARTSVGKLNKRVM 538 +ALVVLK + D VS DI+ HV A+ G IS+YGVP+ VRF+ LARTSVGKLNKR M Sbjct: 482 LALVVLKPEFADQVSAADIQAHVKVFADQGHISKYGVPENVRFLASLARTSVGKLNKRAM 541 Query: 539 REQ 541 REQ Sbjct: 542 REQ 544 >ref|YP_004847238.1| AMP-dependent synthetase and ligase [Pseudogulbenkiania sp. NH8B] dbj|BAK76824.1| AMP-dependent synthetase and ligase [Pseudogulbenkiania sp. NH8B] Length = 547 Score = 705 bits (1819), Expect = 0.0, Method: Composition-based stats. Identities = 357/545 (65%), Positives = 427/545 (78%), Gaps = 7/545 (1%) Query: 3 QTIEQTPSAYGYPLLIKQLLHSALATAPEQEIIYG-ARRLTYAGFYERVQRLANVLKEMG 61 + I TPSAY YPLLIKQLLH+ LA APEQEI+Y RR TY R+ RL + L +G Sbjct: 4 EKITVTPSAYTYPLLIKQLLHTPLAVAPEQEIVYADKRRYTYRELSRRIGRLGHALTRLG 63 Query: 62 VQAGNTVAVMDWDSNRYLECFFAVPMMGCVLQTVNVRLSPEQIAYTLNHAQADVLLVNSD 121 V+ G+TVAVMDWDS+RYLECFF VPM+G VLQ VNVRLSPEQI YTLNHA A+V+LVN D Sbjct: 64 VRGGSTVAVMDWDSHRYLECFFGVPMLGAVLQMVNVRLSPEQILYTLNHAGAEVVLVNQD 123 Query: 122 FMPVLKQIRGELNTLTRCVLLTDDGSPCPQGPGFAGEYEALLAQASAQYDFPDFDENARA 181 F+PVL+++R +L+T VLL+DD P G FAGEYE+LLA QYDFPDFDEN RA Sbjct: 124 FLPVLEELRPQLDTAKTFVLLSDDDIALPGG--FAGEYESLLADCPDQYDFPDFDENTRA 181 Query: 182 TTFYTTGTTGLPKGVYFSHRQLVLHTLGTTAGLALAPQQGRLHRDDVYMPLTPMFHVHAW 241 TTFYTTGTTG PKGVYFSHRQLVLHTL L+L+P+QG HRDDVYMPLTPMFHVHAW Sbjct: 182 TTFYTTGTTGQPKGVYFSHRQLVLHTLAVCNTLSLSPEQGGFHRDDVYMPLTPMFHVHAW 241 Query: 242 GLPYVATMAGVKQVYPGRYVPDTICKLIREEKVTFSHCVPTILQMVLTSEQAREVDLSGW 301 GLPY+AT+AGVKQVYPGRY P +I KLIR+E VTF+H VPTIL M+L + +R+VDLS Sbjct: 242 GLPYIATLAGVKQVYPGRYHPSSILKLIRDEGVTFTHSVPTILTMLLVAPLSRDVDLSRL 301 Query: 302 KMIIGGSAMTGSLARMARDRGIDVFTGYGMSETCPILTLAQVPTEDLGS---EREAVLRV 358 K++IGGSA+T +LAR A +RGID+F GYGMSETCP++ LAQ+ +L + E + RV Sbjct: 302 KVVIGGSALTSALARQALERGIDIFAGYGMSETCPVVALAQLDLAELAAADEEHQVATRV 361 Query: 359 KTGRPVPLVALRTVDGDMNDCPRDGKSTGEVVVRAPWLTQGYLHQAEASEELWRGGWLHT 418 TGRPV LV LRTVD M CP+DGK+ GEVV+RAPW + GY + +ASE+LW GGWLHT Sbjct: 362 CTGRPVSLVDLRTVDARMESCPKDGKTAGEVVLRAPWASMGYYNNPQASEQLWSGGWLHT 421 Query: 419 QDIGHLDAEGYLQVTDRIKDVIKTGGEWVSSLQIEDIIARHPAVAETAVIGVQDGKWGER 478 QDIGH+D+ GYLQVTDRIKDVIKTGGEWVSSL++E++++RHP V E AVIGV D KWGER Sbjct: 422 QDIGHIDSRGYLQVTDRIKDVIKTGGEWVSSLELEEMLSRHPGVREAAVIGVPDEKWGER 481 Query: 479 PVALVVLKKD-VDAVSEEDIRQHVMAAAEAGTISRYGVPDRVRFVPELARTSVGKLNKRV 537 P+ALVV D A++ E+++ HVM E G IS+Y VP+RV FV +LA+TSVGKLNK Sbjct: 482 PLALVVPNPDYAGALTVEELQAHVMTFVEQGLISKYAVPERVLFVEQLAKTSVGKLNKLA 541 Query: 538 MREQY 542 +R QY Sbjct: 542 LRSQY 546 >ref|YP_284122.1| acyl-CoA synthetase [Dechloromonas aromatica RCB] gb|AAZ45652.1| AMP-dependent synthetase and ligase [Dechloromonas aromatica RCB] Length = 552 Score = 686 bits (1770), Expect = 0.0, Method: Composition-based stats. Identities = 353/545 (64%), Positives = 424/545 (77%), Gaps = 7/545 (1%) Query: 1 MP-QTIEQTPSAYGYPLLIKQLLHSALATAPEQEIIY-GARRLTYAGFYERVQRLANVLK 58 MP + ++ T SAY YPLLIKQLL + LA APEQEI Y G R Y ER+ RLANVL Sbjct: 1 MPIKLVQSTVSAYQYPLLIKQLLLTPLAIAPEQEITYQGKLRYNYRTLNERIGRLANVLT 60 Query: 59 EMGVQAGNTVAVMDWDSNRYLECFFAVPMMGCVLQTVNVRLSPEQIAYTLNHAQADVLLV 118 +GV++G TVA+MDWDS+RYLECFFAVPMMG +LQTVNVRLSPEQI YTLNHA+ADVLLV Sbjct: 61 GLGVESGQTVAMMDWDSHRYLECFFAVPMMGAILQTVNVRLSPEQILYTLNHAKADVLLV 120 Query: 119 NSDFMPVLKQIRGELNTLTRCVLLTDDGSPCPQGP-GFAGEYEALLAQASAQYDFPDFDE 177 NS+F +L QI G+L T+ +L++D+ P P GEYEALLA+AS YDFPDFDE Sbjct: 121 NSEFFAILTQIAGQLETVKTIILISDEAE-LPIAPVAMVGEYEALLAEASPDYDFPDFDE 179 Query: 178 NARATTFYTTGTTGLPKGVYFSHRQLVLHTLGTTAGLALAPQQGRLHRDDVYMPLTPMFH 237 NA+ATTFYTTGTTG PKGVYFSHRQLVLHTLG A L A +QG +HR+DVYMP+TPMFH Sbjct: 180 NAQATTFYTTGTTGNPKGVYFSHRQLVLHTLGCAAALGGANRQGHIHREDVYMPMTPMFH 239 Query: 238 VHAWGLPYVATMAGVKQVYPGRYVPDTICKLIREEKVTFSHCVPTILQMVLTSEQAREVD 297 VHAWG PY+ATM GVKQVYPGRY P+ IC+LI E V+FSHCVPTIL M+L+ A+ D Sbjct: 240 VHAWGFPYLATMMGVKQVYPGRYQPEVICRLISTEGVSFSHCVPTILHMILSHPHAKTAD 299 Query: 298 LSGWKMIIGGSAMTGSLARMARDRGIDVFTGYGMSETCPILTLAQVPTEDLGS--EREAV 355 +G K++IGG+AM ++A A+ RG+D+FTGYGMSETCP+L++A + + DL E +AV Sbjct: 300 FTGLKILIGGAAMPEAMAISAQKRGMDLFTGYGMSETCPVLSMAHLESADLNRSLEEQAV 359 Query: 356 LRVKTGRPVPLVALRTVDGDMNDCPRDGKSTGEVVVRAPWLTQGYLHQAEASEELWRGGW 415 +R KTGRP+PLV LR VD +M D P DGK+TGEVVVRAPWLTQGY+ E SE LW GG+ Sbjct: 360 IRAKTGRPLPLVDLRIVDPEMADVPHDGKATGEVVVRAPWLTQGYVSAPEDSEGLWAGGY 419 Query: 416 LHTQDIGHLDAEGYLQVTDRIKDVIKTGGEWVSSLQIEDIIARHPAVAETAVIGVQDGKW 475 LHT DIG+++ GYL+VTDRIKDVIKTGGEW SSLQ+EDIIA+H AV E AVIGV D KW Sbjct: 420 LHTADIGNINPAGYLKVTDRIKDVIKTGGEWTSSLQLEDIIAKHEAVNEVAVIGVPDDKW 479 Query: 476 GERPVALVVLKKD-VDAVSEEDIRQHVMAAAEAGTISRYGVPDRVRFVPELARTSVGKLN 534 GERP+ALV+LK + V ++E +R + A EA ISRYGV +V FV L +TSVGK+N Sbjct: 480 GERPLALVLLKPEHVGQITEHALRNYAAHAIEAAGISRYGVLLQVSFVKTLVKTSVGKMN 539 Query: 535 KRVMR 539 KR+MR Sbjct: 540 KRLMR 544 >gb|EGW62605.1| AMP-dependent synthetase and ligase [Dechlorosoma suillum PS] Length = 558 Score = 684 bits (1766), Expect = 0.0, Method: Composition-based stats. Identities = 357/553 (64%), Positives = 427/553 (77%), Gaps = 8/553 (1%) Query: 1 MP-QTIEQTPSAYGYPLLIKQLLHSALATAPEQEIIY-GARRLTYAGFYERVQRLANVLK 58 MP Q IE TPSAY YPLLIK LLH+ LA AP+QEI+Y + R TY +R+ RLAN L Sbjct: 1 MPVQLIETTPSAYSYPLLIKHLLHTPLAHAPDQEIVYRDSVRYTYRTLQQRIGRLANALA 60 Query: 59 EMGVQAGNTVAVMDWDSNRYLECFFAVPMMGCVLQTVNVRLSPEQIAYTLNHAQADVLLV 118 +GV+ G+TVA+MDWDS+RYLECFFAVPMMG VLQTVNVRL+ EQ+ YTLNHA+ADV+LV Sbjct: 61 ALGVEPGHTVAMMDWDSHRYLECFFAVPMMGAVLQTVNVRLTAEQMLYTLNHARADVVLV 120 Query: 119 NSDFMPVLKQIRGELNTLTRCVLLTDD--GSPCPQGP-GFAGEYEALLAQASAQYDFPDF 175 NS+F P+L+ + L ++ VL+ D Q P FAGEYE LLA A+ YDFPDF Sbjct: 121 NSEFFPLLQAMAERLESVKTFVLIHGDHENPEKMQAPVSFAGEYEELLAAAATDYDFPDF 180 Query: 176 DENARATTFYTTGTTGLPKGVYFSHRQLVLHTLGTTAGLALAPQQGRLHRDDVYMPLTPM 235 DENA+ATTFYTTGTTGLPKGVYFSHRQLV+HT+G GL AP QGRLHRDDVYMP+TPM Sbjct: 181 DENAKATTFYTTGTTGLPKGVYFSHRQLVMHTMGAATGLGTAPVQGRLHRDDVYMPITPM 240 Query: 236 FHVHAWGLPYVATMAGVKQVYPGRYVPDTICKLIREEKVTFSHCVPTILQMVLTSEQARE 295 FHVHAWGLPYVATM G+KQVYPGRY P+ + +L E+VTFSHCVPTIL M+L ARE Sbjct: 241 FHVHAWGLPYVATMLGLKQVYPGRYQPEVLLRLKARERVTFSHCVPTILHMLLNHALARE 300 Query: 296 VDLSGWKMIIGGSAMTGSLARMARDRGIDVFTGYGMSETCPILTLAQVPTEDLGS--ERE 353 DLSGWK++IGGSA+ ++A A ++GIDVFTGYGMSETCPIL++A +PT L + + Sbjct: 301 TDLSGWKVMIGGSALPQAMAVAALEKGIDVFTGYGMSETCPILSVAHLPTAKLQAPLAEQ 360 Query: 354 AVLRVKTGRPVPLVALRTVDGDMNDCPRDGKSTGEVVVRAPWLTQGYLHQAEASEELWRG 413 A LR +TG P+PLV LR VD +M P DG++ GE+VVR+PWLTQGYL A+EELW G Sbjct: 361 AALRARTGLPLPLVDLRIVDPEMRPQPHDGQAVGELVVRSPWLTQGYLKDPAAAEELWGG 420 Query: 414 GWLHTQDIGHLDAEGYLQVTDRIKDVIKTGGEWVSSLQIEDIIARHPAVAETAVIGVQDG 473 G+LHT DIG++DA G +Q+TDRIKDVIKTGGEW SSLQ+EDII +HPAV E AVIGV+D Sbjct: 421 GYLHTADIGNIDANGSIQITDRIKDVIKTGGEWTSSLQLEDIIGQHPAVHEVAVIGVKDE 480 Query: 474 KWGERPVALVVLKKDVDA-VSEEDIRQHVMAAAEAGTISRYGVPDRVRFVPELARTSVGK 532 KWGERP+ALVVLK +++ V+E IR H AE +SRYGV +V FV LARTS+GK Sbjct: 481 KWGERPLALVVLKPEMEGQVTEHAIRNHAAHLAETLGVSRYGVLMQVSFVKTLARTSIGK 540 Query: 533 LNKRVMREQYPAA 545 +NKR MRE AA Sbjct: 541 INKRFMRENLEAA 553