BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.0208.p (419 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003774999.1| major facilitator superfamily permease [Herb... 568 e-160 ref|YP_004155445.1| major facilitator superfamily protein [Vario... 390 e-106 ref|YP_002944786.1| major facilitator superfamily protein [Vario... 370 e-100 ref|YP_262078.2| major facilitator family transporter [Pseudomon... 288 1e-75 ref|YP_001669338.1| major facilitator transporter [Pseudomonas p... 278 1e-72 >ref|YP_003774999.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] gb|ADJ63091.1| permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 383 Score = 568 bits (1464), Expect = e-160, Method: Composition-based stats. Identities = 361/382 (94%), Positives = 368/382 (96%) Query: 37 MTTPDHRQADIDSRHAAMSLSAALPCDIVLYLLLPMYAAQFHVSLAEAGVLLAANRLVRI 96 MTTPD RQAD+ SRHAAMSLSAALPCDIVLYLLLPMYA QFH+SLAEAGVLLAANRLVRI Sbjct: 1 MTTPDLRQADLHSRHAAMSLSAALPCDIVLYLLLPMYADQFHISLAEAGVLLAANRLVRI 60 Query: 97 AGYRWVASFYAQRGDRPTCTLAVCAAILCALGYTVASGFWALLPLRLLWGMAFAGLNLST 156 AGYRWVA FYAQRGDRPTCTLAVCAAI+CALGYT ASGFWALLPLRLLWGMAFAGLNLST Sbjct: 61 AGYRWVARFYAQRGDRPTCTLAVCAAIVCALGYTAASGFWALLPLRLLWGMAFAGLNLST 120 Query: 157 QAMSTANPQGAARRSGRSRGVIAIGPMLWLPTAALLAPHIGPRMVFALVAATALLGLLAA 216 QAMST+NPQGAARRSGRSRGVIAIGPMLWLPTAALLAPHIGPR+VFALVAATALLGLLAA Sbjct: 121 QAMSTSNPQGAARRSGRSRGVIAIGPMLWLPTAALLAPHIGPRLVFALVAATALLGLLAA 180 Query: 217 RRLPQHAYAPPQPRRWKLPNALDWWSFLEGLTLDGLFIVGLAYMSKQSFGADGELVAGVL 276 RRLPQHAYAPPQPRRWKLPNALDWWSFLEGLTLDGLFIVGLAYMSKQSFGADGELVAGVL Sbjct: 181 RRLPQHAYAPPQPRRWKLPNALDWWSFLEGLTLDGLFIVGLAYMSKQSFGADGELVAGVL 240 Query: 277 MALRYAGEIVLSPLGGWLAERFGAVKVLVTLSLATCVALVGFGGGLLWLFAGLIVILRAL 336 MALRYAGEIVLSPLGGWLAERFGAVKVLVTLSLATCVALVGFGGGLLWLFAG+IVILRAL Sbjct: 241 MALRYAGEIVLSPLGGWLAERFGAVKVLVTLSLATCVALVGFGGGLLWLFAGVIVILRAL 300 Query: 337 QLPLLAPIVAARTPAAQRVHALAGRAIWRDIGAGMGPMLAGLLLPVMSSLWLYSLAASAL 396 QLPLLAPIVAART QRVHALAGRAIWRDIGAGMGPMLAGLLLPVMSSLWLYSLAASAL Sbjct: 301 QLPLLAPIVAARTAPEQRVHALAGRAIWRDIGAGMGPMLAGLLLPVMSSLWLYSLAASAL 360 Query: 397 ALAAIACGRQPVPVPPASLSGQ 418 ALAAIACG P PVP S+S Q Sbjct: 361 ALAAIACGGAPAPVPSVSVSSQ 382 >ref|YP_004155445.1| major facilitator superfamily protein [Variovorax paradoxus EPS] gb|ADU37334.1| major facilitator superfamily MFS_1 [Variovorax paradoxus EPS] Length = 393 Score = 390 bits (1003), Expect = e-106, Method: Composition-based stats. Identities = 237/365 (64%), Positives = 289/365 (79%), Gaps = 1/365 (0%) Query: 46 DIDSRHAAMSLSAALPCDIVLYLLLPMYAAQFHVSLAEAGVLLAANRLVRIAGYRWVASF 105 D++SRHAA++L +LP D+VLYLLLPMYA QF V+LAEAG+LLAANRLVRIAGYRWVA F Sbjct: 17 DLNSRHAAIALGLSLPADVVLYLLLPMYADQFGVTLAEAGMLLAANRLVRIAGYRWVARF 76 Query: 106 YAQRGDRPTCTLAVCAAILCALGYTVASGFWALLPLRLLWGMAFAGLNLSTQAMSTANPQ 165 YA+ GDRPTCT+AV AA +C +GY SGFWALLPLRL+WG+ FA LNLSTQA++TA+P Sbjct: 77 YARNGDRPTCTIAVVAAAVCGVGYATLSGFWALLPLRLMWGLCFAALNLSTQALATADPV 136 Query: 166 GAARRSGRSRGVIAIGPMLWLPTAALLAPHIGPRMVFALVAATALLGLLAARRLPQHAYA 225 GAARR+GRSR IA+GP+L LP AL+A +GPR +F ++A +LL + AARRLP + Sbjct: 137 GAARRNGRSRAFIAMGPVLALPLGALMAHWVGPRAIFGILAVFSLLAVFAARRLPSAPHR 196 Query: 226 PPQP-RRWKLPNALDWWSFLEGLTLDGLFIVGLAYMSKQSFGADGELVAGVLMALRYAGE 284 +P RR++ PN+LD WSF+EGL LDGLFIVGL+Y+ K +VAG+LMALRY E Sbjct: 197 TEKPTRRFQWPNSLDGWSFMEGLALDGLFIVGLSYLGKDLLPGGAVIVAGLLMALRYLAE 256 Query: 285 IVLSPLGGWLAERFGAVKVLVTLSLATCVALVGFGGGLLWLFAGLIVILRALQLPLLAPI 344 I LSP+GG LA+RFGA ++LV+LSL T +ALVGFG G LW A LIV+LRALQLPLL+PI Sbjct: 257 IFLSPVGGHLADRFGAERLLVSLSLVTAIALVGFGLGWLWSCAALIVVLRALQLPLLSPI 316 Query: 345 VAARTPAAQRVHALAGRAIWRDIGAGMGPMLAGLLLPVMSSLWLYSLAASALALAAIACG 404 VA RTP +RV ALA R++WRDIGAG GP++AGLLLPV S WLY AA LA+ A+ACG Sbjct: 317 VARRTPGPERVQALAARSVWRDIGAGAGPLIAGLLLPVASPPWLYGSAAVLLAIMALACG 376 Query: 405 RQPVP 409 R P P Sbjct: 377 RNPSP 381 >ref|YP_002944786.1| major facilitator superfamily protein [Variovorax paradoxus S110] gb|ACS19520.1| major facilitator superfamily MFS_1 [Variovorax paradoxus S110] Length = 390 Score = 370 bits (950), Expect = e-100, Method: Composition-based stats. Identities = 236/375 (62%), Positives = 286/375 (76%), Gaps = 3/375 (0%) Query: 40 PDHRQADIDSRHAAMSLSAALPCDIVLYLLLPMYAAQFHVSLAEAGVLLAANRLVRIAGY 99 P D++SRHAA++L +LP D+VLYLLLPMYA QF V+LAEAG+LLAANRLVRIAGY Sbjct: 8 PAPAATDLNSRHAAIALGLSLPADVVLYLLLPMYAGQFGVTLAEAGMLLAANRLVRIAGY 67 Query: 100 RWVASFYAQRGDRPTCTLAVCAAILCALGYTVASGFWALLPLRLLWGMAFAGLNLSTQAM 159 VA FYA+ GDR +CT+AV AA C LGY SGFWALLPLRL+WG+ FA LNLSTQ++ Sbjct: 68 GAVARFYARHGDRLSCTIAVVAAAFCGLGYATLSGFWALLPLRLVWGLCFAALNLSTQSL 127 Query: 160 STANPQGAARRSGRSRGVIAIGPMLWLPTAALLAPHIGPRMVFALVAATALLGLLAARRL 219 +TA GAARRSGRSR +IA+GP+L LP ALLA GPR++F ++AA +L+ +LA RRL Sbjct: 128 ATAELLGAARRSGRSRALIAMGPVLALPLGALLALWAGPRIIFGILAAFSLVAVLATRRL 187 Query: 220 PQHAYAPPQPR---RWKLPNALDWWSFLEGLTLDGLFIVGLAYMSKQSFGADGELVAGVL 276 P + P R R+ PN+LD WSF+EGLTLDGLFIVGL+Y+ K +VAG L Sbjct: 188 PAAPHGSPHSRPARRFGRPNSLDAWSFMEGLTLDGLFIVGLSYLGKDLMPGGAVVVAGSL 247 Query: 277 MALRYAGEIVLSPLGGWLAERFGAVKVLVTLSLATCVALVGFGGGLLWLFAGLIVILRAL 336 MALRY EI+LSP+GG +AERFGA ++LV+LSL T +AL GFG G LW A IV+LRAL Sbjct: 248 MALRYLAEILLSPVGGRMAERFGAERLLVSLSLVTAIALAGFGLGWLWSCAASIVVLRAL 307 Query: 337 QLPLLAPIVAARTPAAQRVHALAGRAIWRDIGAGMGPMLAGLLLPVMSSLWLYSLAASAL 396 QLPLL PIVA RTP +R+HALA RA+WRD+GAG+GPMLAGLLLPV++S WLY + A L Sbjct: 308 QLPLLPPIVAHRTPGPERMHALAARAVWRDVGAGIGPMLAGLLLPVVASSWLYGICALLL 367 Query: 397 ALAAIACGRQPVPVP 411 A AA+ACGR P P Sbjct: 368 AAAALACGRNPSASP 382 >ref|YP_262078.2| major facilitator family transporter [Pseudomonas fluorescens Pf-5] gb|AAY94227.2| transporter, major facilitator family [Pseudomonas fluorescens Pf-5] Length = 386 Score = 288 bits (738), Expect = 1e-75, Method: Composition-based stats. Identities = 206/362 (56%), Positives = 258/362 (71%), Gaps = 1/362 (0%) Query: 43 RQADIDSRHAAMSLSAALPCDIVLYLLLPMYAAQFHVSLAEAGVLLAANRLVRIAGYRWV 102 R D S AA L LP D +LYLLLPM F V+LAEAG+LLAANRLVRI GY V Sbjct: 7 RTTDKASARAASILGLCLPGDTLLYLLLPMQHEAFGVTLAEAGLLLAANRLVRIFGYSHV 66 Query: 103 ASFYAQRGDRPTCTLAVCAAILCALGYTVASGFWALLPLRLLWGMAFAGLNLSTQAMSTA 162 FYA+RGDRP C LA A LCALGY SGFW LL LRL WG+ +A LNLSTQ M+TA Sbjct: 67 MRFYARRGDRPVCVLAAATAALCALGYATLSGFWWLLLLRLGWGLTYAALNLSTQVMATA 126 Query: 163 NPQGAARRSGRSRGVIAIGPMLWLPTAALLAPHIGPRMVFALVAATALLGLLAARRLPQH 222 P GAARR+GRSR +IA GPML LP AL++ GPR++F L+A T+L+ LL A LP Sbjct: 127 EPTGAARRTGRSRALIATGPMLALPLGALVSVEYGPRVIFLLMALTSLVALLLAFGLPSK 186 Query: 223 AYA-PPQPRRWKLPNALDWWSFLEGLTLDGLFIVGLAYMSKQSFGADGELVAGVLMALRY 281 + RR++ P+++ WSF+EGL LDGLFI+GL+ ++Q + LVAG L+ALRY Sbjct: 187 PHPLASSSRRFQRPDSVALWSFIEGLVLDGLFIIGLSLQAEQLMAGNAVLVAGGLLALRY 246 Query: 282 AGEIVLSPLGGWLAERFGAVKVLVTLSLATCVALVGFGGGLLWLFAGLIVILRALQLPLL 341 E++LSPLGG A+R+GA ++L+ SL T VALVGFG L + L+++LRALQLPL+ Sbjct: 247 VSEMLLSPLGGLAAQRWGATRMLIVFSLLTSVALVGFGCHWLIVGGALVLVLRALQLPLV 306 Query: 342 APIVAARTPAAQRVHALAGRAIWRDIGAGMGPMLAGLLLPVMSSLWLYSLAASALALAAI 401 +VA R P A RV ALA A+WRDIGAG+GP++AGLLLPV S++W+YSLAA LA++A+ Sbjct: 307 VTVVAQRHPGAGRVRALAANAVWRDIGAGVGPLIAGLLLPVASTVWVYSLAAGCLAVSAL 366 Query: 402 AC 403 AC Sbjct: 367 AC 368 >ref|YP_001669338.1| major facilitator transporter [Pseudomonas putida GB-1] gb|ABY99002.1| major facilitator superfamily MFS_1 [Pseudomonas putida GB-1] Length = 378 Score = 278 bits (711), Expect = 1e-72, Method: Composition-based stats. Identities = 213/373 (57%), Positives = 270/373 (72%), Gaps = 4/373 (1%) Query: 35 ASMTTPDHRQ-ADIDSRHAAMSLSAALPCDIVLYLLLPMYAAQFHVSLAEAGVLLAANRL 93 +S+ TP Q AD DSR+AA LS LP D++LYLLLPMYAA F V+LAEAGVLLAANRL Sbjct: 2 SSVATPVQTQRADRDSRNAAQVLSLCLPSDVLLYLLLPMYAADFGVTLAEAGVLLAANRL 61 Query: 94 VRIAGYRWVASFYAQRGDRPTCTLAVCAAILCALGYTVASGFWALLPLRLLWGMAFAGLN 153 VRI GY WV FYA+ GDR C+LA AA +CALG + +GF ALL LRL+WG+ FA N Sbjct: 62 VRIIGYGWVVRFYARHGDRAACSLAAFAAAVCALGNAMLTGFAALLLLRLVWGLCFATFN 121 Query: 154 LSTQAMSTANPQGAARRSGRSRGVIAIGPMLWLPTAALLAPHIGPRMVFALVAATALLGL 213 LSTQ ++TA QGAARR+GRSR ++IGPML LP AL+A GPR VF ++ AL GL Sbjct: 122 LSTQTLATAEVQGAARRAGRSRATLSIGPMLALPLGALMAQAYGPRSVFFVLCIGALFGL 181 Query: 214 LAARRLP--QHAYAPPQPRRWKLPNALDWWSFLEGLTLDGLFIVGLAYMSKQSFGADGEL 271 AR LP HA A RR +LP+++ WSF+EG+TLDGLFI GL+ ++ G G L Sbjct: 182 WRARALPGTGHAVASRGGRRLRLPDSIATWSFIEGVTLDGLFIFGLSLYAQTHLGESGVL 241 Query: 272 VAGVLMALRYAGEIVLSPLGGWLAERFGAVKVLVTLSLATCVALVGFGGGLLWLFAGLIV 331 AGVLMA+RY E++ SP GG LA+RFG +++LV LSLAT +AL+ F L++ A ++ Sbjct: 242 AAGVLMAVRYLSEMLFSPFGGRLADRFGPLRMLVALSLATSLALLLFASHWLFIGAFFVL 301 Query: 332 ILRALQLPLLAPIVAARTPAAQRVHALAGRAIWRDIGAGMGPMLAGLLLPVMSSLWLYSL 391 +LRALQLPL+ +VA R P QR+ ALAG A+WRDIGAG+GPMLAG+LLP + ++W Y+ Sbjct: 302 VLRALQLPLVMTLVALRNP-QQRIQALAGNAVWRDIGAGLGPMLAGVLLPQVPAIWAYAG 360 Query: 392 AASALALAAIACG 404 AA A+A+AA+ C Sbjct: 361 AALAIAMAALNCA 373