BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.0190.p (482 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_002945293.1| major facilitator superfamily protein [Vario... 672 0.0 ref|YP_003277145.1| major facilitator superfamily protein [Comam... 549 e-154 ref|YP_004549391.1| major facilitator superfamily protein [Sinor... 543 e-152 ref|NP_386183.1| putative transport protein [Sinorhizobium melil... 543 e-152 ref|YP_002007739.1| general substrate transporter; major facilit... 541 e-152 >ref|YP_002945293.1| major facilitator superfamily protein [Variovorax paradoxus S110] gb|ACS20027.1| major facilitator superfamily MFS_1 [Variovorax paradoxus S110] Length = 484 Score = 672 bits (1735), Expect = 0.0, Method: Composition-based stats. Identities = 385/485 (79%), Positives = 430/485 (88%), Gaps = 5/485 (1%) Query: 1 MAGPTLSSPDLPHSPRVAELDGALDKIGVTRSHHTIIFLILIGCLFDAFEQNAIGLVGPL 60 MAGP LSS P SPRVAELD ALD IGVTRSHHTIIFLILIGCLFDAFEQNA+G+VGP+ Sbjct: 1 MAGPALSSG--PDSPRVAELDRALDTIGVTRSHHTIIFLILIGCLFDAFEQNAVGIVGPM 58 Query: 61 LKQQWGLTATDIGLLNTVTFASAAIGRIVSGLVADRYGRRVMLSLDLLLFTLGAGICALA 120 L++QWGL+A+DIG LNTVTFA AA GRIVSG +ADRYGRRVMLS+DLLLFTLGAGICA+A Sbjct: 59 LREQWGLSASDIGFLNTVTFACAATGRIVSGFIADRYGRRVMLSIDLLLFTLGAGICAMA 118 Query: 121 PDLTVMAVGRAIVGFGLGGEIAIAVTMLAEFCSSKFRGTAVGLVNVGAGGMGNFLAPAFG 180 P+ TVMA+GRA+VGFGLGGEIAIAVTMLAEFCS+KFRGTAVGLVNVGAGG+GNFLAPAFG Sbjct: 119 PNFTVMALGRAVVGFGLGGEIAIAVTMLAEFCSTKFRGTAVGLVNVGAGGLGNFLAPAFG 178 Query: 181 LLVFTLFPGENSWRWLFFCLMLPALLGAFYRRYIPETPRFLLSRNKIAEANQVLARLASG 240 LLVF +FPG+N+WRWLF CLM+PALLGAFYRR+IPETPRFLLS+ ++ E N VL+RLASG Sbjct: 179 LLVFWMFPGDNAWRWLFACLMVPALLGAFYRRFIPETPRFLLSQGRVRETNAVLSRLASG 238 Query: 241 KLSGPAPQGRDWISDDGSRQQTAPAKVRSMEIFQGALARRTLPLCIAIWMTYGAQISVLT 300 +LSG R++I+DD + Q A KVR EIF+GALARRT LCI IWMTYGAQISVLT Sbjct: 239 RLSGKLAAEREYITDDRTAAQPARPKVRVTEIFRGALARRTAALCITIWMTYGAQISVLT 298 Query: 301 LMPTILVSLGYTISKSLLFTMVMQSGSLLGAIAASVFGFHLPRKRVLTAGAICACLAALA 360 LMPTILV+LGY++SKSL+FTMVMQ GSLLGAIAAS+ GFH PRKRVLTAGA+CACLAALA Sbjct: 299 LMPTILVTLGYSMSKSLMFTMVMQGGSLLGAIAASLLGFHFPRKRVLTAGAVCACLAALA 358 Query: 361 IGFLAKSIVLILAAGAVFQFFVLLLNTTIWIFAPELYPTRVRAFGTAFILATGTAAGALM 420 IGF+AKSI +IL AGAVFQFFVLLLNTTIWIFAPELYPTRVRAFGTAFILATGTAAGALM Sbjct: 359 IGFVAKSIAVILVAGAVFQFFVLLLNTTIWIFAPELYPTRVRAFGTAFILATGTAAGALM 418 Query: 421 PLVAGRLFDAFGLAGVFGMIAAMYGIFTVAIRTVPETYGKSPDA-PLE-ETEPEAVVPAV 478 PLVAGRLFDAFGL GVFG+ AAMY +F +++ VPETYG+SPDA PL E EP PA Sbjct: 419 PLVAGRLFDAFGLVGVFGLAAAMYAVFVASVQCVPETYGQSPDALPLAGEAEPGDGAPAA 478 Query: 479 A-ARS 482 + ARS Sbjct: 479 SLARS 483 >ref|YP_003277145.1| major facilitator superfamily protein [Comamonas testosteroni CNB-2] gb|ACY31849.1| major facilitator superfamily MFS_1 [Comamonas testosteroni CNB-2] Length = 544 Score = 549 bits (1414), Expect = e-154, Method: Composition-based stats. Identities = 288/461 (62%), Positives = 369/461 (80%), Gaps = 4/461 (0%) Query: 5 TLSSPDLPHSPRVAELDGALDKIGVTRSHHTIIFLILIGCLFDAFEQNAIGLVGPLLKQQ 64 T S+ + ++ +L+ AL++IGVTRSH +IIFL++ G LFD FEQNA+GLVGPLL+QQ Sbjct: 55 TPSASGKASAAQMQQLENALNQIGVTRSHKSIIFLVVCGVLFDVFEQNAVGLVGPLLRQQ 114 Query: 65 WGLTATDIGLLNTVTFASAAIGRIVSGLVADRYGRRVMLSLDLLLFTLGAGICALAPDLT 124 WGL ATDI LNT+TF +AA+GR++SG +ADR GRR ML+++L LFTLGA CALAPD Sbjct: 115 WGLDATDIAFLNTITFFAAAMGRLLSGYLADRMGRRFMLNVNLALFTLGAVGCALAPDYA 174 Query: 125 VMAVGRAIVGFGLGGEIAIAVTMLAEFCSSKFRGTAVGLVNVGAGGMGNFLAPAFGLLVF 184 +AV RAIVGFGLGGEI AVTMLAEFCS++FRGTAVGL+NVG GG+GN LAPAF L VF Sbjct: 175 FLAVARAIVGFGLGGEITTAVTMLAEFCSARFRGTAVGLINVGGGGLGNMLAPAFALGVF 234 Query: 185 TLFPGENSWRWLFFCLMLPALLGAFYRRYIPETPRFLLSRNKIAEANQVLARLASGKLSG 244 TLFPG++SWRWLF CL++PA+ FYRR++PETPRFLLS+ ++ EANQVL+ LA+GKL+ Sbjct: 235 TLFPGDDSWRWLFGCLVMPAIFIVFYRRFVPETPRFLLSKGRVEEANQVLSMLAAGKLAN 294 Query: 245 PAPQGRDWISDDGSRQQTAPAKV--RS--MEIFQGALARRTLPLCIAIWMTYGAQISVLT 300 + R++I+ +++QTA A+ RS +IF+G ARRT+ + +A WMT+GAQ+SVLT Sbjct: 295 AQYELREFITPGSAQEQTAAAQTVQRSSFTDIFRGGYARRTIAVGVASWMTFGAQLSVLT 354 Query: 301 LMPTILVSLGYTISKSLLFTMVMQSGSLLGAIAASVFGFHLPRKRVLTAGAICACLAALA 360 LMPTILV+ GY+I+KS FT+VMQ GSLLGAIAAS G++ PRKRVLT GAI ACLA LA Sbjct: 355 LMPTILVAQGYSITKSFSFTIVMQMGSLLGAIAASTMGYYFPRKRVLTVGAIAACLAGLA 414 Query: 361 IGFLAKSIVLILAAGAVFQFFVLLLNTTIWIFAPELYPTRVRAFGTAFILATGTAAGALM 420 G S+ +IL GA+FQF VL LNT+IWIFAPELYPTR+RA GT+F+LA GT GA+ Sbjct: 415 FGNFTDSVAMILVCGALFQFCVLTLNTSIWIFAPELYPTRIRALGTSFLLALGTIGGAMS 474 Query: 421 PLVAGRLFDAFGLAGVFGMIAAMYGIFTVAIRTVPETYGKS 461 ++AG++FD G++G+FGMIAAMY IF +A++ PET+G+S Sbjct: 475 QVLAGKMFDLHGVSGMFGMIAAMYFIFAIAVQFAPETFGRS 515 >ref|YP_004549391.1| major facilitator superfamily protein [Sinorhizobium meliloti AK83] gb|AEG04806.1| major facilitator superfamily MFS_1 [Sinorhizobium meliloti BL225C] gb|AEG53777.1| major facilitator superfamily MFS_1 [Sinorhizobium meliloti AK83] Length = 487 Score = 543 bits (1399), Expect = e-152, Method: Composition-based stats. Identities = 287/462 (62%), Positives = 366/462 (79%), Gaps = 4/462 (0%) Query: 14 SPRVAELDGALDKIGVTRSHHTIIFLILIGCLFDAFEQNAIGLVGPLLKQQWGLTATDIG 73 SP + +L+ A KIGVT +H I+ L+LIGCLFD+FEQN IG+ GP+LK+ WGLT TDIG Sbjct: 13 SPVIRQLESAFTKIGVTGAHKQILALVLIGCLFDSFEQNTIGVAGPILKEHWGLTGTDIG 72 Query: 74 LLNTVTFASAAIGRIVSGLVADRYGRRVMLSLDLLLFTLGAGICALAPDLTVMAVGRAIV 133 LNT+TF SAAIGR++SG++ DRYGRRVML+++LLLFT+G+ CA+AP+ T++ RAIV Sbjct: 73 FLNTITFGSAAIGRLLSGILGDRYGRRVMLTINLLLFTIGSAACAMAPNFTMLCFARAIV 132 Query: 134 GFGLGGEIAIAVTMLAEFCSSKFRGTAVGLVNVGAGGMGNFLAPAFGLLVFTLFPGENSW 193 GFG+GGEI+ AVTML+EFCS KFRGTA GLVNVGAGG GNFLAPAFGLL+FTLFPGENSW Sbjct: 133 GFGVGGEISTAVTMLSEFCSPKFRGTAAGLVNVGAGGFGNFLAPAFGLLIFTLFPGENSW 192 Query: 194 RWLFFCLMLPALLGAFYRRYIPETPRFLLSRNKIAEANQVLARLASGKLSGPAPQGRDWI 253 RWLF L LPALL FYRR++PETPRFL S+NKI EAN+VL+ LASG L +++ Sbjct: 193 RWLFASLALPALLVVFYRRFVPETPRFLASKNKIGEANKVLSILASGSLKPRNLVVHEYL 252 Query: 254 SDDGSRQQTAPAKVRSMEIFQGALARRTLPLCIAIWMTYGAQISVLTLMPTILVSLGYTI 313 + D + AP K E+F+ RT+P+ IAI M+YGAQ+SVLTLMP I VS+GYT+ Sbjct: 253 TTDHMTDE-APEKSDWRELFRAPFIGRTIPVAIAILMSYGAQLSVLTLMPVIFVSMGYTL 311 Query: 314 SKSLLFTMVMQSGSLLGAIAASVFGFHLPRKRVLTAGAICACLAALAIGFLAKSIVLILA 373 S SLL++M++QSGS+LGAIAAS+FG++LPRK+VLT GA ACLAA++I L +I L+L Sbjct: 312 SGSLLYSMIIQSGSVLGAIAASMFGYYLPRKKVLTVGAALACLAAVSIVTLGTNIYLVLL 371 Query: 374 AGAVFQFFVLLLNTTIWIFAPELYPTRVRAFGTAFILATGTAAGALMPLVAGRLFDAFGL 433 GA+FQFFVLLLNT+IWI+APEL+PTR+RAFG A ILATG+AAG+ +P ++G LFD++G+ Sbjct: 372 FGAIFQFFVLLLNTSIWIYAPELFPTRIRAFGVALILATGSAAGSFVPTISGMLFDSYGM 431 Query: 434 AGVFGMIAAMYGIFTVAIRTVPETYGKSPD---APLEETEPE 472 GVFG+ A MY +F I+ PETYG+S + P E +P+ Sbjct: 432 VGVFGLAAGMYAVFAFCIQLGPETYGRSMEDLSQPAEADQPK 473 >ref|NP_386183.1| putative transport protein [Sinorhizobium meliloti 1021] emb|CAC46656.1| Putative transport protein [Sinorhizobium meliloti 1021] Length = 482 Score = 543 bits (1399), Expect = e-152, Method: Composition-based stats. Identities = 287/462 (62%), Positives = 366/462 (79%), Gaps = 4/462 (0%) Query: 14 SPRVAELDGALDKIGVTRSHHTIIFLILIGCLFDAFEQNAIGLVGPLLKQQWGLTATDIG 73 SP + +L+ A KIGVT +H I+ L+LIGCLFD+FEQN IG+ GP+LK+ WGLT TDIG Sbjct: 8 SPVIRQLESAFTKIGVTGAHKQILALVLIGCLFDSFEQNTIGVAGPILKEHWGLTGTDIG 67 Query: 74 LLNTVTFASAAIGRIVSGLVADRYGRRVMLSLDLLLFTLGAGICALAPDLTVMAVGRAIV 133 LNT+TF SAAIGR++SG++ DRYGRRVML+++LLLFT+G+ CA+AP+ T++ RAIV Sbjct: 68 FLNTITFGSAAIGRLLSGILGDRYGRRVMLTINLLLFTIGSAACAMAPNFTMLCFARAIV 127 Query: 134 GFGLGGEIAIAVTMLAEFCSSKFRGTAVGLVNVGAGGMGNFLAPAFGLLVFTLFPGENSW 193 GFG+GGEI+ AVTML+EFCS KFRGTA GLVNVGAGG GNFLAPAFGLL+FTLFPGENSW Sbjct: 128 GFGVGGEISTAVTMLSEFCSPKFRGTAAGLVNVGAGGFGNFLAPAFGLLIFTLFPGENSW 187 Query: 194 RWLFFCLMLPALLGAFYRRYIPETPRFLLSRNKIAEANQVLARLASGKLSGPAPQGRDWI 253 RWLF L LPALL FYRR++PETPRFL S+NKI EAN+VL+ LASG L +++ Sbjct: 188 RWLFASLALPALLVVFYRRFVPETPRFLASKNKIGEANKVLSILASGSLKPRNLVVHEYL 247 Query: 254 SDDGSRQQTAPAKVRSMEIFQGALARRTLPLCIAIWMTYGAQISVLTLMPTILVSLGYTI 313 + D + AP K E+F+ RT+P+ IAI M+YGAQ+SVLTLMP I VS+GYT+ Sbjct: 248 TTDHMTDE-APEKSDWRELFRAPFIGRTIPVAIAILMSYGAQLSVLTLMPVIFVSMGYTL 306 Query: 314 SKSLLFTMVMQSGSLLGAIAASVFGFHLPRKRVLTAGAICACLAALAIGFLAKSIVLILA 373 S SLL++M++QSGS+LGAIAAS+FG++LPRK+VLT GA ACLAA++I L +I L+L Sbjct: 307 SGSLLYSMIIQSGSVLGAIAASMFGYYLPRKKVLTVGAALACLAAVSIVTLGTNIYLVLL 366 Query: 374 AGAVFQFFVLLLNTTIWIFAPELYPTRVRAFGTAFILATGTAAGALMPLVAGRLFDAFGL 433 GA+FQFFVLLLNT+IWI+APEL+PTR+RAFG A ILATG+AAG+ +P ++G LFD++G+ Sbjct: 367 FGAIFQFFVLLLNTSIWIYAPELFPTRIRAFGVALILATGSAAGSFVPTISGMLFDSYGM 426 Query: 434 AGVFGMIAAMYGIFTVAIRTVPETYGKSPD---APLEETEPE 472 GVFG+ A MY +F I+ PETYG+S + P E +P+ Sbjct: 427 VGVFGLAAGMYAVFAFCIQLGPETYGRSMEDLSQPAEADQPK 468 >ref|YP_002007739.1| general substrate transporter; major facilitator superfamily mfs_1 [Cupriavidus taiwanensis LMG 19424] emb|CAQ71682.1| General substrate transporter; Major facilitator superfamily MFS_1 [Cupriavidus taiwanensis LMG 19424] Length = 482 Score = 541 bits (1395), Expect = e-152, Method: Composition-based stats. Identities = 291/454 (64%), Positives = 361/454 (79%), Gaps = 9/454 (1%) Query: 12 PHSPRVAELDGALDKIGVTRSHHTIIFLILIGCLFDAFEQNAIGLVGPLLKQQWGLTATD 71 P+SP+ ELD A+D IGVT SH IIF+I++G +FD FEQNA+GL+GP+L++QWG++ + Sbjct: 10 PNSPQQHELDRAMDGIGVTASHKKIIFMIMLGVMFDVFEQNAVGLIGPMLREQWGISVAE 69 Query: 72 IGLLNTVTFASAAIGRIVSGLVADRYGRRVMLSLDLLLFTLGAGICALAPDLTVMAVGRA 131 IG LNT+TF++AA+GRI SG +ADRYGRR MLS +LLLFTLGA ICALAP+ V+A GR Sbjct: 70 IGFLNTLTFSAAALGRIGSGYIADRYGRRAMLSANLLLFTLGAIICALAPNYWVLAAGRF 129 Query: 132 IVGFGLGGEIAIAVTMLAEFCSSKFRGTAVGLVNVGAGGMGNFLAPAFGLLVFTLFPGEN 191 IVG GLGGEI+IAVTMLAE CS++FRGTAVGLV+VG+GG+GN LAPAFGL VF +FPG + Sbjct: 130 IVGIGLGGEISIAVTMLAELCSTRFRGTAVGLVSVGSGGLGNMLAPAFGLAVFAMFPGPD 189 Query: 192 SWRWLFFCLMLPALLGAFYRRYIPETPRFLLSRNKIAEANQVLARLASGKLSG----PAP 247 SWRWLF CL+LPA FYRR+IPETPRFLLS+ ++ EAN+VL+ LASGKL P P Sbjct: 190 SWRWLFACLVLPAFFVIFYRRFIPETPRFLLSKGRVDEANRVLSVLASGKLGKLDGEPTP 249 Query: 248 QGRDWISDDGSRQQTAPAKVRSMEIFQGALARRTLPLCIAIWMTYGAQISVLTLMPTILV 307 + + D+ R AKVR +IF+G L RRT+ L IA+ MTYGAQISVLTLMPTIL+ Sbjct: 250 YIKAAMQDEAPR-----AKVRLSDIFKGRLGRRTIALGIAVSMTYGAQISVLTLMPTILM 304 Query: 308 SLGYTISKSLLFTMVMQSGSLLGAIAASVFGFHLPRKRVLTAGAICACLAALAIGFLAKS 367 + GYTISKS LFTMVMQSGSL GA+AAS G+H+PRKRVLT GA AC A L GFL + Sbjct: 305 AQGYTISKSFLFTMVMQSGSLFGALAASYCGYHIPRKRVLTVGAGLACAAGLCFGFLTYN 364 Query: 368 IVLILAAGAVFQFFVLLLNTTIWIFAPELYPTRVRAFGTAFILATGTAAGALMPLVAGRL 427 + L+L GA F F V+LLNT+IWIFAPE YPT VRAFGT+ ILA GT AGAL PLV+GR+ Sbjct: 365 VALVLLFGAAFTFCVVLLNTSIWIFAPEQYPTHVRAFGTSLILALGTLAGALTPLVSGRV 424 Query: 428 FDAFGLAGVFGMIAAMYGIFTVAIRTVPETYGKS 461 F+ +G+ G+F M+AAMYG+F +A++ PET+G++ Sbjct: 425 FETYGVGGMFSMLAAMYGVFALAVQFAPETFGRA 458