BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.0180.p (466 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003773745.1| transmembrane protein [Herbaspirillum serope... 802 0.0 ref|ZP_07673754.1| conserved hypothetical protein [Ralstonia sp.... 287 4e-75 ref|YP_001892564.1| transmembrane protein [Ralstonia pickettii 1... 286 5e-75 emb|CBJ41082.1| conserved membrane protein of unknown function [... 282 9e-74 emb|CCA86979.1| conserved membrane hypothetical protein [Ralston... 279 8e-73 >ref|YP_003773745.1| transmembrane protein [Herbaspirillum seropedicae SmR1] gb|ADJ61837.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 529 Score = 802 bits (2071), Expect = 0.0, Method: Composition-based stats. Identities = 415/466 (89%), Positives = 439/466 (94%) Query: 1 MPARYGMAGVLGIAAFSYLAWTWGVWGCWRSASKHVARGGRRWAEVVVKFLIVCGAISMV 60 MPARYGM GVL IAAFSYLAWTWGV GCWRSAS+HVARGGRRWAE+ VKFLI+CG ISM Sbjct: 64 MPARYGMGGVLFIAAFSYLAWTWGVCGCWRSASRHVARGGRRWAELAVKFLILCGVISMA 123 Query: 61 TSTWRGAHSVREQLEIALGKQLGPPVVYQVRVDGKSVLLQGGMNDGAADGLDAILQQHPG 120 TSTWR AHS+REQ EIALGKQLGPPVVYQ+RVDGKSVLLQGGMNDGAADGL IL QHP Sbjct: 124 TSTWRAAHSLREQFEIALGKQLGPPVVYQLRVDGKSVLLQGGMNDGAADGLARILDQHPS 183 Query: 121 VQTVVLYSAGGWVREGKLVGEVIRRRRLNTYVEQECSSSCTLAFLAGRDRAMDPRAHIGF 180 V+TVVLYSAGGWVREGKLVGEVIRRR L TYVEQECSSSCTLAFLAGRDRAMDPRAHIGF Sbjct: 184 VRTVVLYSAGGWVREGKLVGEVIRRRGLATYVEQECSSSCTLAFLAGRDRAMDPRAHIGF 243 Query: 181 HTLYTVGGDDRINKSFDRKLTFDTYGKLGLPHDFIERIADTPAAQSWYPELGQLLQAGVL 240 HTLYTVGGDDRINKSFDRKLTFDTYGKLGLP +FIERIADTPA+ SWYPELGQLLQAGVL Sbjct: 244 HTLYTVGGDDRINKSFDRKLTFDTYGKLGLPREFIERIADTPASSSWYPELGQLLQAGVL 303 Query: 241 TRLAPGGETAQLATIATSRDVLEAEFKKGALFDIMARYHPADFARTIDLAWAKVQARASD 300 TRLAPGGETAQLATIATSRD+LEAEFKKGALFDIMARYHP +FA TIDLAWA+VQA ASD Sbjct: 304 TRLAPGGETAQLATIATSRDILEAEFKKGALFDIMARYHPKEFAHTIDLAWAQVQAHASD 363 Query: 301 AEILAVGREQVGLLSRRLLPIASDASLAEFQQLLLDEAVALRGISAAACTELIYPRSRPE 360 A+ILAVGREQ+G+LSRRLLPIASDASLAEFQQLLLD+AVALRGISAAACTELIYPRSR + Sbjct: 364 AQILAVGREQIGILSRRLLPIASDASLAEFQQLLLDQAVALRGISAAACTELIYPRSRED 423 Query: 361 AAAGREAPQAALPQEFANREQVMTQRLLRDADPANAGRYSLAERTRVMRKALAPLTQEQL 420 AAAGREAPQA LP EFANREQ MTQ LLRDADP NAGRY+LAERTRV+R+ALAPLTQ+QL Sbjct: 424 AAAGREAPQAVLPPEFANREQRMTQHLLRDADPVNAGRYTLAERTRVIRQALAPLTQDQL 483 Query: 421 KLLSSPLRRQADPDATCDASIAYLRAVMQLPAEERRVALRVLYSRS 466 KLLSSPLRRQADP+ATC+ASIAYLRAV QLP +ERRVALRVLYSR+ Sbjct: 484 KLLSSPLRRQADPEATCEASIAYLRAVNQLPPQERRVALRVLYSRN 529 >ref|ZP_07673754.1| conserved hypothetical protein [Ralstonia sp. 5_7_47FAA] gb|EFP67958.1| conserved hypothetical protein [Ralstonia sp. 5_7_47FAA] Length = 538 Score = 287 bits (734), Expect = 4e-75, Method: Composition-based stats. Identities = 177/468 (37%), Positives = 255/468 (54%), Gaps = 11/468 (2%) Query: 2 PARYGMAGVLGIAAFSYLAWTWGVWGCWRSASKHVAR----GGRRWAEVVVKFLIVCGAI 57 PAR + +L I W W V G WR+A + AR G ++ K +IV GA+ Sbjct: 70 PARAASSALLVIYVMGLALWIWAVVGTWRAADREQARNRLAGLSNPWPLIAKGMIVLGAV 129 Query: 58 SMVTSTWRGAHSVREQLEIALGKQLGPPVVYQVRVDGKSVLLQGGMNDGAADGLDAILQQ 117 + + L ALG+Q P + DG+++L GG+NDGAAD L+A L++ Sbjct: 130 GTSSRVVNDGPRLGAHLRTALGEQAASPFAVIPQRDGRAILFSGGINDGAADALEAALRK 189 Query: 118 HPGVQTVVLYSAGGWVREGKLVGEVIRRRRLNTYVEQECSSSCTLAFLAGRDRAMDPRAH 177 P VVL S GGW+REG LV +VIRR L TYVE+ C+S+CT+AFLAG DRA P A Sbjct: 190 APNATAVVLRSEGGWLREGTLVADVIRRHHLRTYVERACASACTIAFLAGEDRAAAPGAR 249 Query: 178 IGFHTLYTVGGDDRINKSFDRKLTFDTYGKLGLPHDFIERIADTPAAQSWYPELGQLLQA 237 +GFH VG + + + Y GLP F+ R+ DTP Q W+P +LL Sbjct: 250 LGFHRPRAVGAEHDQSPGATDSELWRAYSDAGLPDAFVRRVQDTPFDQMWFPTAQELLAD 309 Query: 238 GVLTRLAPGGETAQLATIATSRDVLEAEFKKGALFDIMARYHPADFARTIDLAWAKVQAR 297 V+TR +PGGE A +AT +R+ L AE + L+ + R +PA F+R ID W ++Q Sbjct: 310 HVVTRASPGGEYATMATQLNTREALAAELRSAPLYAALERKYPAHFSRLIDTLWPELQRN 369 Query: 298 ASDAEILAVGREQVGLLSRRLLPIASDASLAEFQQLLLDEAVALRGISAAACTELIYPRS 357 +DA+ + + REQ G L R L+P A +A L QL L++A AL+ +S AC + Sbjct: 370 VTDAQAMTLLREQTGKLYRALIPTAPNALLFANAQLTLEQAQALQRVSPEACVAYL---- 425 Query: 358 RPEAAAGREAPQAALPQEFANREQVMTQRLLRDADPANAGRYSLAERTRVMRKALAPLTQ 417 + G A A LP+ REQ + L++ ADP +A + A+ V++ A+A L Sbjct: 426 --DGTPGASAAAARLPRTLVTREQTLFSDLMQAADPDHAPVVTRAQSLPVLQLAVAALPP 483 Query: 418 EQLKLLSSPLRRQADPDAT-CDASIAYLRAVMQLPAEERRVALRVLYS 464 Q ++L+ P R + P A C A I + R+++ LP ER +ALR +Y+ Sbjct: 484 AQQRVLTVPALRHSTPPAERCQALIGFSRSILALPEAERALALRGMYA 531 >ref|YP_001892564.1| transmembrane protein [Ralstonia pickettii 12J] ref|YP_002984066.1| hypothetical protein Rpic12D_4146 [Ralstonia pickettii 12D] gb|ACD29137.1| putative transmembrane protein [Ralstonia pickettii 12J] gb|ACS65394.1| conserved hypothetical protein [Ralstonia pickettii 12D] Length = 537 Score = 286 bits (732), Expect = 5e-75, Method: Composition-based stats. Identities = 184/469 (39%), Positives = 262/469 (55%), Gaps = 13/469 (2%) Query: 2 PARYGMAGVLGIAAFSYLAWTWGVWGCWRSASKHVAR----GGRRWAEVVVKFLIVCGAI 57 PAR + +L I W W V G WRSA + AR G ++ K +IV GA+ Sbjct: 69 PARAASSALLVIYVMGLALWIWAVVGTWRSADREQARNRLAGLSNPWPLIAKVMIVLGAV 128 Query: 58 SMVTSTWRGAHSVREQLEIALGKQLGPPVVYQVRVDGKSVLLQGGMNDGAADGLDAILQQ 117 + + L ALG+Q P + DG+++L GG+NDGAAD L+A L++ Sbjct: 129 GTCSRLINDGPRLVAHLRTALGEQAASPYAVMPQRDGRAILFNGGINDGAADALEAALRK 188 Query: 118 HPGVQTVVLYSAGGWVREGKLVGEVIRRRRLNTYVEQECSSSCTLAFLAGRDRAMDPRAH 177 P VVL S GGW+REG LV +VIRR L TYVE+ C+S+CT+AFLAG DRA P A Sbjct: 189 APNATAVVLRSEGGWLREGTLVADVIRRHHLRTYVERACASACTIAFLAGADRAAAPGAR 248 Query: 178 IGFHTLYTVGGD-DRINKSFDRKLTFDTYGKLGLPHDFIERIADTPAAQSWYPELGQLLQ 236 IGFH VG D D+ + D +L + Y GLP F+ R+ DTP Q W+P +LL Sbjct: 249 IGFHRPRAVGADHDQTPGATDSEL-WRAYSDAGLPDAFVRRVQDTPFDQMWFPTAQELLA 307 Query: 237 AGVLTRLAPGGETAQLATIATSRDVLEAEFKKGALFDIMARYHPADFARTIDLAWAKVQA 296 V+TR +PGGE A +AT +R+ L AE + L+ + R +PA F+R ID ++Q Sbjct: 308 NHVVTRTSPGGEYATMATQLNTREALAAELRSAPLYATLERKYPAHFSRLIDTLGPELQR 367 Query: 297 RASDAEILAVGREQVGLLSRRLLPIASDASLAEFQQLLLDEAVALRGISAAACTELIYPR 356 +DA+++ + REQ G L R L+P A DA L QL L++A AL+ +S AC + Sbjct: 368 NVTDAQVVTLLREQTGKLYRALIPTAPDALLFANAQLTLEQAQALQRVSPEACVAYL--- 424 Query: 357 SRPEAAAGREAPQAALPQEFANREQVMTQRLLRDADPANAGRYSLAERTRVMRKALAPLT 416 + +G A A LP+ REQ + L++ ADP +A + A+ V++ A+A L Sbjct: 425 ---DGTSGASAAAARLPRALVTREQALFSDLMQAADPDHAPVVTRAQTLPVLQLAMAALP 481 Query: 417 QEQLKLLSSP-LRRQADPDATCDASIAYLRAVMQLPAEERRVALRVLYS 464 + ++L+ P LRR A P C A I + R+++ LP ER +ALR +Y+ Sbjct: 482 LNEQRVLTVPALRRTAPPAERCRALIGFSRSILALPEAERALALRGMYA 530 >emb|CBJ41082.1| conserved membrane protein of unknown function [Ralstonia solanacearum CMR15] Length = 537 Score = 282 bits (721), Expect = 9e-74, Method: Composition-based stats. Identities = 180/469 (38%), Positives = 254/469 (54%), Gaps = 13/469 (2%) Query: 2 PARYGMAGVLGIAAFSYLAWTWGVWGCWRSASKHVARGGRR-----WAEVVVKFLIVCGA 56 PAR + +L I AF + W W + G WR+A + A R W +V K +I+ GA Sbjct: 69 PARAASSALLLIYAFGLVLWVWAIVGTWRAAEREQAEKQRAGLSGVWCKVA-KLMILLGA 127 Query: 57 ISMVTSTWRGAHSVREQLEIALGKQLGPPVVYQVRVDGKSVLLQGGMNDGAADGLDAILQ 116 I + + + ALG+Q P + DG++VL GG+NDGAAD L+ L+ Sbjct: 128 IGTGSRVVNELPRLGAHVRTALGEQAASPFAVTPQRDGRAVLFTGGINDGAADALEQALR 187 Query: 117 QHPGVQTVVLYSAGGWVREGKLVGEVIRRRRLNTYVEQECSSSCTLAFLAGRDRAMDPRA 176 + PG TVVL S GGW+REG LV EVIRR RL+TYVE+ C+S+CT+AFLAG DRA P A Sbjct: 188 KTPGATTVVLRSEGGWLREGTLVAEVIRRHRLHTYVERTCASACTIAFLAGDDRAAAPGA 247 Query: 177 HIGFHTLYTVGGDDRINKSFDRKLTFDTYGKLGLPHDFIERIADTPAAQSWYPELGQLLQ 236 IGFH +G D + Y GLP F+ + TP W+P +LL Sbjct: 248 RIGFHRPRAIGADQDQAPGATDSALWSAYAAAGLPDAFVRHVQATPFDAIWFPTDRELLD 307 Query: 237 AGVLTRLAPGGETAQLATIATSRDVLEAEFKKGALFDIMARYHPADFARTIDLAWAKVQA 296 GV+TR + GGE A +AT+ T+R+ L AE + L+ + R +PA F +D W ++ Sbjct: 308 NGVVTRASSGGEYATMATLLTTREALSAELRTAPLYATLERKYPAHFKSLLDNIWPALRR 367 Query: 297 RASDAEILAVGREQVGLLSRRLLPIASDASLAEFQQLLLDEAVALRGISAAACTELIYPR 356 ASDAEI+A R Q L R LLP A DA L +L +D+A L+ IS AC + Sbjct: 368 SASDAEIIAQLRAQSSRLYRALLPTAPDALLLANARLTIDQAKTLQRISPEACVGYLDGT 427 Query: 357 SRPEAAAGREAPQAALPQEFANREQVMTQRLLRDADPANAGRYSLAERTRVMRKALAPLT 416 + P A R LP REQ + L++ ADP +A + A+ ++ A+A L Sbjct: 428 AGPGTIAAR------LPDALLTREQALFSDLMQAADPEHAPMVTRAQAMPALQLAIAALP 481 Query: 417 QEQLKLLSSP-LRRQADPDATCDASIAYLRAVMQLPAEERRVALRVLYS 464 ++ ++L+ P LR A A C A I + +A++ LP +R +ALR +Y+ Sbjct: 482 MQEQRVLTFPTLRHTAPAAARCRALIDFAQAILSLPDADRVLALRGIYA 530 >emb|CCA86979.1| conserved membrane hypothetical protein [Ralstonia syzygii R24] Length = 536 Score = 279 bits (713), Expect = 8e-73, Method: Composition-based stats. Identities = 181/470 (38%), Positives = 256/470 (54%), Gaps = 15/470 (3%) Query: 2 PARYGMAGVLGIAAFSYLAWTWGVWGCWRSASKHVARGGRR-----WAEVVVKFLIVCGA 56 PAR + +L I + W W + G WR+A + A R W+ V K +I+ GA Sbjct: 68 PARAASSALLVIYTLGLILWVWAIVGTWRAAERERAEKQRAGLSGVWS-TVAKLMILLGA 126 Query: 57 ISMVTSTWRGAHSVREQLEIALGKQLGPPVVYQVRVDGKSVLLQGGMNDGAADGLDAILQ 116 I + + L ALG+Q P + DG++VL GG+NDGAAD L+ L+ Sbjct: 127 IGTGSRVVNDLPRLGAHLRTALGEQAASPFTVTPQRDGRAVLFTGGINDGAADTLEQALR 186 Query: 117 QHPGVQTVVLYSAGGWVREGKLVGEVIRRRRLNTYVEQECSSSCTLAFLAGRDRAMDPRA 176 + PG TVVL S GGW+REG LV EVIRR RL+TYVE+ C+S+CT+AFLAG DRA P A Sbjct: 187 KTPGATTVVLRSEGGWLREGALVAEVIRRHRLHTYVERTCASACTIAFLAGDDRAAAPGA 246 Query: 177 HIGFHTLYTVGGD-DRINKSFDRKLTFDTYGKLGLPHDFIERIADTPAAQSWYPELGQLL 235 IGFH +G D D+ + D +L + Y GLP+ F+ + T W+P +LL Sbjct: 247 RIGFHRPRAIGADQDQAPGATDSEL-WRAYAAAGLPNAFVRHVQATSFDTMWFPTARELL 305 Query: 236 QAGVLTRLAPGGETAQLATIATSRDVLEAEFKKGALFDIMARYHPADFARTIDLAWAKVQ 295 GV+TR + GGE A +AT+ T+R+ L E + L+ + R +PA F +D W +Q Sbjct: 306 DNGVVTRASSGGEYATMATLLTTREALATELRTAPLYATLERKYPAHFKSLLDSIWPALQ 365 Query: 296 ARASDAEILAVGREQVGLLSRRLLPIASDASLAEFQQLLLDEAVALRGISAAACTELIYP 355 ASDAEI+A R Q L R LLP A D L QL +D+A L+ IS AC + Sbjct: 366 RNASDAEIIAQLRTQSSRLYRALLPTAPDTLLLANAQLTIDQAETLQRISPEACVGYLDG 425 Query: 356 RSRPEAAAGREAPQAALPQEFANREQVMTQRLLRDADPANAGRYSLAERTRVMRKALAPL 415 + P A R LP REQ + L++ ADP +A + A+ ++ A+A L Sbjct: 426 TAGPGTVAAR------LPGTLLTREQALFSDLVQAADPEHAPMVTRAQAMPALQLAIAAL 479 Query: 416 TQEQLKLLSSP-LRRQADPDATCDASIAYLRAVMQLPAEERRVALRVLYS 464 ++ ++L+ P LR A A C A I + +A++ LP +R +ALR +Y+ Sbjct: 480 PVQEQRVLTFPTLRHTAPAAARCRALINFSQAILALPDADRALALRGIYA 529