BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0084.p (420 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_004752826.1| cysteine desulfurase [Collimonas fungivorans... 564 e-158 ref|YP_003774982.1| cysteine desulfurase [Herbaspirillum seroped... 539 e-151 ref|YP_004684938.1| cysteine desulfurase IscS [Cupriavidus necat... 464 e-128 ref|YP_295280.1| cysteine desulfurase IscS [Ralstonia eutropha J... 462 e-128 ref|YP_725667.1| cysteine desulfurase [Ralstonia eutropha H16] >... 461 e-127 >ref|YP_004752826.1| cysteine desulfurase [Collimonas fungivorans Ter331] gb|AEK62003.1| Cysteine desulfurase [Collimonas fungivorans Ter331] Length = 397 Score = 564 bits (1453), Expect = e-158, Method: Compositional matrix adjust. Identities = 272/364 (74%), Positives = 312/364 (85%), Gaps = 1/364 (0%) Query: 1 MTASSACIPITSSGQDQTPLQFPIYLDYSATTPVDPLVATRMFAYLTEKFGNPASSSHSF 60 MTA+ + +S +P Q PIYLDYSATTPVDP VA +M YLTEKFGNPASSSH+F Sbjct: 1 MTAACTTVVPDNSDAAASP-QLPIYLDYSATTPVDPAVAVKMCEYLTEKFGNPASSSHAF 59 Query: 61 GWEAKAAVEKARKQVAALVGAQASEIVWTSGATESNNLALKSAAHALRAAGRGKHLITVA 120 GWEAKAAVE+AR+QVAALVGAQASEIVWTSGATESNNLALK AAH LR GRG+HL+T++ Sbjct: 60 GWEAKAAVEQARRQVAALVGAQASEIVWTSGATESNNLALKGAAHVLRERGRGRHLVTMS 119 Query: 121 TEHKAVLDTMHTLERAGFEVSFLQPGTDGLITVAQFAEALRPDTILASVMMVNNEIGVIQ 180 TEHKAVLDTM TLER GFEV++L P +GL+ + F ALR DTI+ASVMMVNNEIGVIQ Sbjct: 120 TEHKAVLDTMRTLEREGFEVTYLAPQPNGLVDLETFKAALRSDTIIASVMMVNNEIGVIQ 179 Query: 181 PIEELGEICAARDILFHVDAVQAVGKIAIDLATLKVDLMSFSAHKIYGPKGIGALFVRNN 240 P+ ELGEICA RDI+FHVDA QA GK+AIDL +LKV+LMSFSAHK+YGPKGIGALFVR++ Sbjct: 180 PVAELGEICAGRDIVFHVDAAQAAGKVAIDLRSLKVNLMSFSAHKVYGPKGIGALFVRHD 239 Query: 241 PALRLEAQIDGGGHENGMRSGTLPTHQIVGMGEAFALAANLQDTEAARIGVLRDRLWEGL 300 PAL+LEAQIDGGGHENGMRSGTL THQIVGMGEAF+LAA L D+E ARI L++RLW+GL Sbjct: 240 PALKLEAQIDGGGHENGMRSGTLATHQIVGMGEAFSLAAKLLDSENARIRSLQERLWDGL 299 Query: 301 KDLPGVVLNGSATARIPHNLNVSFDLDGEISVAGQLLDIAVSAGSACNSANAAPSYVLTA 360 +D PGVVLNG R+PHNLN+SF++ G +A QLLDIAVSAGSACNSANAAPSYVL++ Sbjct: 300 RDCPGVVLNGDRLQRVPHNLNLSFEIPGSTPIAAQLLDIAVSAGSACNSANAAPSYVLSS 359 Query: 361 LGRP 364 LGRP Sbjct: 360 LGRP 363 >ref|YP_003774982.1| cysteine desulfurase [Herbaspirillum seropedicae SmR1] gb|ADJ63074.1| cysteine desulfurase protein [Herbaspirillum seropedicae SmR1] Length = 408 Score = 539 bits (1388), Expect = e-151, Method: Compositional matrix adjust. Identities = 271/363 (74%), Positives = 295/363 (81%), Gaps = 7/363 (1%) Query: 1 MTASSACIPITSSGQDQTPLQFPIYLDYSATTPVDPLVATRMFAYLTEKFGNPASSSHSF 60 M A + C P L+FPIYLDY ATTPVDP VA M +LT+KFGN ASSSH F Sbjct: 1 MNAPAPCRPPAD-------LRFPIYLDYGATTPVDPQVARVMCDFLTDKFGNAASSSHPF 53 Query: 61 GWEAKAAVEKARKQVAALVGAQASEIVWTSGATESNNLALKSAAHALRAAGRGKHLITVA 120 GWEA+ AVE+AR QVA LVGAQ EIVWTSGATESNNLALK AA+ALRA GRG HLITV Sbjct: 54 GWEARRAVEQARAQVAGLVGAQPGEIVWTSGATESNNLALKGAAYALRAQGRGNHLITVP 113 Query: 121 TEHKAVLDTMHTLERAGFEVSFLQPGTDGLITVAQFAEALRPDTILASVMMVNNEIGVIQ 180 TEHKAVLDTMHTLE+AGFEV++LQPG DGLIT Q ALRPDT+LASVM VNNEIGVIQ Sbjct: 114 TEHKAVLDTMHTLEKAGFEVTYLQPGADGLITPQQLRAALRPDTLLASVMYVNNEIGVIQ 173 Query: 181 PIEELGEICAARDILFHVDAVQAVGKIAIDLATLKVDLMSFSAHKIYGPKGIGALFVRNN 240 PI ELGEICAA D++FHVDAVQA GKIAIDL LKVDLMSFSAHK+YGPKGIGAL+VR+N Sbjct: 174 PIAELGEICAAHDVVFHVDAVQAAGKIAIDLTRLKVDLMSFSAHKVYGPKGIGALYVRHN 233 Query: 241 PALRLEAQIDGGGHENGMRSGTLPTHQIVGMGEAFALAANLQDTEAARIGVLRDRLWEGL 300 P L+LEAQIDGGGHENGMRSGTL THQIVGMGEAFALA L + E+ RIG LRDRLW GL Sbjct: 234 PQLKLEAQIDGGGHENGMRSGTLATHQIVGMGEAFALAGRLLEEESRRIGALRDRLWAGL 293 Query: 301 KDLPGVVLNGSATARIPHNLNVSFDLDGEISVAGQLLDIAVSAGSACNSANAAPSYVLTA 360 DLPGV LNG A R+PHNLNVSF ++ QLL IAVSAGSACNSA AAPS+VL A Sbjct: 294 ADLPGVQLNGCARQRVPHNLNVSFPPTRHGTLGSQLLGIAVSAGSACNSAAAAPSFVLLA 353 Query: 361 LGR 363 +GR Sbjct: 354 IGR 356 >ref|YP_004684938.1| cysteine desulfurase IscS [Cupriavidus necator N-1] gb|AEI76457.1| cysteine desulfurase IscS [Cupriavidus necator N-1] Length = 405 Score = 464 bits (1194), Expect = e-128, Method: Compositional matrix adjust. Identities = 229/347 (65%), Positives = 270/347 (77%), Gaps = 5/347 (1%) Query: 18 TPLQFPIYLDYSATTPVDPLVATRMFAYLTEKFGNPASSSHSFGWEAKAAVEKARKQVAA 77 TP FPIY+DYSATTPVDP VA +M YL E+FGNPAS SH++GWEA+ AVE+AR+QVAA Sbjct: 3 TP-HFPIYMDYSATTPVDPRVADKMIPYLREQFGNPASRSHAYGWEAERAVEEAREQVAA 61 Query: 78 LVGAQASEIVWTSGATESNNLALKSAAHALRAAGRGKHLITVATEHKAVLDTMHTLERAG 137 LVGA EIVWTSGATESNNLA+K AA+ +G+GKHLIT+ TEHKAVLDT LER G Sbjct: 62 LVGADPREIVWTSGATESNNLAIKGAANFY--SGKGKHLITLKTEHKAVLDTTRELERQG 119 Query: 138 FEVSFLQPGTDGLITVAQFAEALRPDTILASVMMVNNEIGVIQPIEELGEICAARDILFH 197 FEV++L +GL+ + F +ALRPDTIL SVM+VNNEIGVIQ +E++GEIC + I+FH Sbjct: 120 FEVTYLDVKENGLLDMEVFKQALRPDTILVSVMLVNNEIGVIQDVEQIGEICREKGIIFH 179 Query: 198 VDAVQAVGKIAIDLATLKVDLMSFSAHKIYGPKGIGALFVRNNPALRLEAQIDGGGHENG 257 DA QA GK+AIDL LK DLMSFSAHK YGPKGIGAL+VR P +R+EAQ+ GGGHE G Sbjct: 180 CDAAQATGKVAIDLNKLKCDLMSFSAHKTYGPKGIGALYVRRKPRVRIEAQMHGGGHERG 239 Query: 258 MRSGTLPTHQIVGMGEAFALAANLQDTEAARIGVLRDRLWEGLKDLPGVVLNGSATARIP 317 MRSGTL THQIVGMGEAF +A TE RI +LRDRLW GL D+ V LNG R+P Sbjct: 240 MRSGTLATHQIVGMGEAFRIAREEMATENERIRMLRDRLWNGLSDMEEVYLNGDLEQRVP 299 Query: 318 HNLNVSFDL-DGEISVAGQLLDIAVSAGSACNSANAAPSYVLTALGR 363 HNLNVSF+ +GE S+ + D+AVS+GSAC SA+ PSYVL ALGR Sbjct: 300 HNLNVSFNFVEGE-SLIMAIKDVAVSSGSACTSASLEPSYVLRALGR 345 >ref|YP_295280.1| cysteine desulfurase IscS [Ralstonia eutropha JMP134] gb|AAZ60436.1| cysteine desulfurase IscS [Ralstonia eutropha JMP134] Length = 405 Score = 462 bits (1188), Expect = e-128, Method: Compositional matrix adjust. Identities = 230/347 (66%), Positives = 268/347 (77%), Gaps = 5/347 (1%) Query: 18 TPLQFPIYLDYSATTPVDPLVATRMFAYLTEKFGNPASSSHSFGWEAKAAVEKARKQVAA 77 TP FPIY+DYSATTPVDP VA +M YL E+FGNPAS SH++GWEA+ AVE+AR+QVAA Sbjct: 3 TP-HFPIYMDYSATTPVDPRVADKMIPYLREQFGNPASRSHAYGWEAERAVEEAREQVAA 61 Query: 78 LVGAQASEIVWTSGATESNNLALKSAAHALRAAGRGKHLITVATEHKAVLDTMHTLERAG 137 LVGA EIVWTSGATESNNLA+K AA+ +G+GKHLITV TEHKAVLDT LER G Sbjct: 62 LVGADPREIVWTSGATESNNLAIKGAANFY--SGKGKHLITVKTEHKAVLDTTRELERQG 119 Query: 138 FEVSFLQPGTDGLITVAQFAEALRPDTILASVMMVNNEIGVIQPIEELGEICAARDILFH 197 FEV++L +GLI + +A+RPDTIL SVM VNNEIGVIQ IE++GEIC + I+FH Sbjct: 120 FEVTYLDVKENGLIDMDVLKQAIRPDTILVSVMFVNNEIGVIQDIEQIGEICREKGIIFH 179 Query: 198 VDAVQAVGKIAIDLATLKVDLMSFSAHKIYGPKGIGALFVRNNPALRLEAQIDGGGHENG 257 DA QA GK+ IDL LKVDLMSFSAHK YGPKGIGAL+VR P +R+EAQ+ GGGHE G Sbjct: 180 CDAAQATGKVVIDLNKLKVDLMSFSAHKTYGPKGIGALYVRRKPRVRIEAQMHGGGHERG 239 Query: 258 MRSGTLPTHQIVGMGEAFALAANLQDTEAARIGVLRDRLWEGLKDLPGVVLNGSATARIP 317 MRSGTL THQIVGMGEAF +A TE RI +LRDRLW GLK + V LNG R+P Sbjct: 240 MRSGTLATHQIVGMGEAFRIAREEMATENERIRMLRDRLWNGLKSMEEVYLNGDLEQRVP 299 Query: 318 HNLNVSFDL-DGEISVAGQLLDIAVSAGSACNSANAAPSYVLTALGR 363 HNLNVSF+ +GE S+ + D+AVS+GSAC SA+ PSYVL ALGR Sbjct: 300 HNLNVSFNFVEGE-SLIMAIKDVAVSSGSACTSASLEPSYVLRALGR 345 >ref|YP_725667.1| cysteine desulfurase [Ralstonia eutropha H16] emb|CAJ92299.1| cysteine desulfurase (IscS) [Ralstonia eutropha H16] Length = 405 Score = 461 bits (1185), Expect = e-127, Method: Compositional matrix adjust. Identities = 227/347 (65%), Positives = 269/347 (77%), Gaps = 5/347 (1%) Query: 18 TPLQFPIYLDYSATTPVDPLVATRMFAYLTEKFGNPASSSHSFGWEAKAAVEKARKQVAA 77 TP FPIY+DYSATTPVDP VA +M YL E+FGNPAS SH++GWEA+ AVE+AR+QVAA Sbjct: 3 TP-HFPIYMDYSATTPVDPRVADKMIPYLREQFGNPASRSHAYGWEAERAVEEAREQVAA 61 Query: 78 LVGAQASEIVWTSGATESNNLALKSAAHALRAAGRGKHLITVATEHKAVLDTMHTLERAG 137 LVGA EIVWTSGATESNNLA+K AA+ +G+GKH+IT+ TEHKAVLDT LER G Sbjct: 62 LVGADPREIVWTSGATESNNLAIKGAANFY--SGKGKHIITLKTEHKAVLDTTRELERQG 119 Query: 138 FEVSFLQPGTDGLITVAQFAEALRPDTILASVMMVNNEIGVIQPIEELGEICAARDILFH 197 FEV++L +GL+ + F +ALRPDTIL SVM+VNNEIGVIQ + ++GEIC + I+FH Sbjct: 120 FEVTYLDVKENGLLDMEVFKQALRPDTILVSVMLVNNEIGVIQDVAQIGEICREKGIIFH 179 Query: 198 VDAVQAVGKIAIDLATLKVDLMSFSAHKIYGPKGIGALFVRNNPALRLEAQIDGGGHENG 257 DA QA GK+AIDL LK DLMSFSAHK YGPKGIGAL+VR P +R+EAQ+ GGGHE G Sbjct: 180 CDAAQATGKVAIDLGKLKCDLMSFSAHKTYGPKGIGALYVRRKPRVRIEAQMHGGGHERG 239 Query: 258 MRSGTLPTHQIVGMGEAFALAANLQDTEAARIGVLRDRLWEGLKDLPGVVLNGSATARIP 317 MRSGTL THQIVGMGEAF +A TE RI +LRDRLW GL D+ V LNG R+P Sbjct: 240 MRSGTLATHQIVGMGEAFRIAREEMATENERIRMLRDRLWNGLSDMEEVYLNGDLEQRVP 299 Query: 318 HNLNVSFDL-DGEISVAGQLLDIAVSAGSACNSANAAPSYVLTALGR 363 HNLNVSF+ +GE S+ + D+AVS+GSAC SA+ PSYVL ALGR Sbjct: 300 HNLNVSFNFVEGE-SLIMAIKDVAVSSGSACTSASLEPSYVLRALGR 345