BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0011.p (255 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003775947.1| sphingosine kinase/eukaryotic diacylglycerol... 335 3e-90 ref|YP_001619945.1| hypothetical protein sce9292 [Sorangium cell... 201 6e-50 ref|NP_109525.1| hypothetical protein mll9677 [Mesorhizobium lot... 155 6e-36 ref|NP_108258.1| hypothetical protein mll8080 [Mesorhizobium lot... 152 3e-35 ref|YP_003527117.1| diacylglycerol kinase [Nitrosococcus halophi... 142 5e-32 >ref|YP_003775947.1| sphingosine kinase/eukaryotic diacylglycerol kinase [Herbaspirillum seropedicae SmR1] gb|ADJ64039.1| sphingosine kinase/eukaryotic diacylglycerol kinase protein [Herbaspirillum seropedicae SmR1] Length = 339 Score = 335 bits (860), Expect = 3e-90, Method: Compositional matrix adjust. Identities = 168/230 (73%), Positives = 186/230 (80%), Gaps = 15/230 (6%) Query: 6 KAAFGVLPLGTLNHFAKDLNIPLALDDAIRNIVEGRRLAIDTGEVNGVLFINNSSLGLYP 65 ++A GVLPLGTLNHFAKDLNIPL L+ AI NI GRRL +D+ EVNGV FINNSSLGLYP Sbjct: 100 QSALGVLPLGTLNHFAKDLNIPLTLEGAIANIATGRRLRVDSAEVNGVAFINNSSLGLYP 159 Query: 66 DIVRDREKQQNRLGRGKWLAFCWAAMGALRRYPFLDVRLDVLMGTKDGGNAESHHRRTPF 125 DIVR+REKQQ RLGRGKWLAF WAAMGALRRYPFL VRL + + + H RRTPF Sbjct: 160 DIVREREKQQARLGRGKWLAFTWAAMGALRRYPFLRVRLSI--------DGQEHWRRTPF 211 Query: 126 VFIGNNEYLMDGLNIGKRNSLSEGKLSLYVCHRTGRLGLVRLALHALFGRLRQARDFDML 185 VFIGNN YLM GL+IGKR+SL+EG LSLYVCH TGR GL+RLALHALFGRLRQA DFDML Sbjct: 212 VFIGNNPYLMSGLDIGKRSSLTEGCLSLYVCHHTGRWGLLRLALHALFGRLRQAHDFDML 271 Query: 186 SAAEIEIDTRHRHGQHRIKRLRVATDGEVTIMQTPLVYRIRPASLTVIVP 235 +A +I I+T R R+RVATDGEV +MQTPL YRIRPASL VIVP Sbjct: 272 TATDIHIETHKR-------RMRVATDGEVQLMQTPLSYRIRPASLEVIVP 314 >ref|YP_001619945.1| hypothetical protein sce9292 [Sorangium cellulosum 'So ce 56'] emb|CAN99465.1| hypothetical protein sce9292 [Sorangium cellulosum 'So ce 56'] Length = 374 Score = 201 bits (512), Expect = 6e-50, Method: Compositional matrix adjust. Identities = 103/234 (44%), Positives = 147/234 (62%), Gaps = 15/234 (6%) Query: 2 VSGSKAAFGVLPLGTLNHFAKDLNIPLALDDAIRNIVEGRRLAIDTGEVNGVLFINNSSL 61 ++G+ GVLPLGT+NHFAK L +PLAL+ A+R +VEGRR+ +D GEVNG +F+NNS+L Sbjct: 132 IAGTDKTMGVLPLGTVNHFAKHLGVPLALEGAVRTLVEGRRVRVDVGEVNGRVFVNNSTL 191 Query: 62 GLYPDIVRDREKQQNRLGRGKWLAFCWAAMGALRRYPFLDVRLDVLMGTKDGGNAESHHR 121 G+YP IVR RE + R G KW+A + + +RR+ + +RL + DG E Sbjct: 192 GIYPGIVRAREALRKRRGLNKWIALALSTVSGIRRHERVRLRLVL-----DGQEVELE-- 244 Query: 122 RTPFVFIGNNEYLMDGLNIGKRNSLSEGKLSLYVCHRTGRLGLVRLALHALFGRLRQARD 181 TPFVF+ NN++ + ++IG++ G+L + V H RL ++L A+ GR+ Q+ D Sbjct: 245 -TPFVFVANNDFKLGDMDIGRQRPPCSGELGVCVAHAESRLSTLKLIASAVVGRIHQSND 303 Query: 182 FDMLSAAEIEIDTRHRHGQHRIKRLRVATDGEVTIMQTPLVYRIRPASLTVIVP 235 F +L E+ ++ HR RL VA DGEV M PL YR+RPA+L VIVP Sbjct: 304 FSILRTTELRVEAPHR-------RLHVAADGEVLRMAPPLCYRVRPAALRVIVP 350 >ref|NP_109525.1| hypothetical protein mll9677 [Mesorhizobium loti MAFF303099] dbj|BAB54864.1| mll9677 [Mesorhizobium loti MAFF303099] Length = 480 Score = 155 bits (392), Expect = 6e-36, Method: Compositional matrix adjust. Identities = 95/240 (39%), Positives = 133/240 (55%), Gaps = 18/240 (7%) Query: 1 VVSGSKAAFGVLPLGTLNHFAKDLNIPLALDDAIRNIVEGRRLAIDTGEVNGVLFINNSS 60 ++G+ GVLPLGTLNHFAKDL +PL L AI I G+ +A+D EVNG +F+NNSS Sbjct: 247 ALAGTDVVLGVLPLGTLNHFAKDLGVPLDLTAAIDTIATGKPVAVDVAEVNGRVFLNNSS 306 Query: 61 LGLYPDIVRDREKQQNRLGRGKWLAFCWAAMGALRRYPFLDVRLDVLMGTKDGGNAESHH 120 +G+YP V R +Q R G GK A A M LR + V + GT++ Sbjct: 307 IGIYPFFVAKRSAEQRRRGLGKLAAIGPALMRTLRFASWQAVHV-AAQGTRE-------R 358 Query: 121 RRTPFVFIGNNEYLMDGLNIGKRNSLSEGKLSLYVCHRTGRLGLVRLALHALFGRLRQAR 180 RTP VF+GNN Y D ++G+R SLS +L +YV + GL L FG + R Sbjct: 359 LRTPCVFVGNNFY--DIADLGRRRSLSSKELCVYVVKQQSWFGLALLPFKIAFGMVDATR 416 Query: 181 DFDMLSAAEIEIDTRHRHGQHRIKRLRVATDGEVTIMQTPLVYRIRPASLTVIVPT-VPD 239 D ++ ++I T HR + + ++ DGE + TPL ++IRPA+L V P +P+ Sbjct: 417 DLEIYRTNSLQI-TSHR------RAMLISLDGEAVSINTPLNFQIRPAALQVFAPAKIPE 469 >ref|NP_108258.1| hypothetical protein mll8080 [Mesorhizobium loti MAFF303099] dbj|BAB53719.1| mll8080 [Mesorhizobium loti MAFF303099] Length = 285 Score = 152 bits (385), Expect = 3e-35, Method: Compositional matrix adjust. Identities = 91/234 (38%), Positives = 125/234 (53%), Gaps = 17/234 (7%) Query: 2 VSGSKAAFGVLPLGTLNHFAKDLNIPLALDDAIRNIVEGRRLAIDTGEVNGVLFINNSSL 61 ++G+ GVLPLGTLNHFAKDL +PL L AI I G+ +A+D EVNG +F+NNSS+ Sbjct: 59 LAGTDVVLGVLPLGTLNHFAKDLGVPLDLTAAIDTIATGKPVAVDVAEVNGRVFLNNSSI 118 Query: 62 GLYPDIVRDREKQQNRLGRGKWLAFCWAAMGALRRYPFLDVRLDVLMGTKDGGNAESHHR 121 G+YP V R +Q R G GK A A M LR + V + Sbjct: 119 GIYPFFVAKRSAEQRRRGFGKLAAIGPALMRTLRFASWQAVHVS--------AQGARERL 170 Query: 122 RTPFVFIGNNEYLMDGLNIGKRNSLSEGKLSLYVCHRTGRLGLVRLALHALFGRLRQARD 181 RTP VF+GNN Y D ++G+R SL +L +YV + GL L FG + RD Sbjct: 171 RTPCVFVGNNFY--DIADLGRRRSLPSKELCVYVVKQQSWFGLALLPFKIAFGMVDATRD 228 Query: 182 FDMLSAAEIEIDTRHRHGQHRIKRLRVATDGEVTIMQTPLVYRIRPASLTVIVP 235 ++ ++I T HR + + ++ DGE + TPL ++IRPA+L V P Sbjct: 229 LEIYRTNSLQI-TSHR------RAMLISLDGEAVSINTPLNFQIRPATLQVFAP 275 >ref|YP_003527117.1| diacylglycerol kinase [Nitrosococcus halophilus Nc4] gb|ADE14730.1| diacylglycerol kinase catalytic region [Nitrosococcus halophilus Nc4] Length = 303 Score = 142 bits (358), Expect = 5e-32, Method: Compositional matrix adjust. Identities = 92/232 (39%), Positives = 127/232 (54%), Gaps = 14/232 (6%) Query: 2 VSGSKAAFGVLPLGTLNHFAKDLNIPLALDDAIRNIVEGRRLAIDTGEVNGVLFINNSSL 61 +S + G+LPLGT NHF++ L IPL LD+A++ I +G R+ +D EVNG FINN+S+ Sbjct: 80 LSQTSITLGILPLGTFNHFSRTLGIPLILDEAVKVIAKGARILVDVAEVNGYTFINNASV 139 Query: 62 GLYPDIVRDREKQQNRLGRGKWLAFCWAAMGALRRYPFLDVRLDVLMGTKDGGNAESHHR 121 G+Y VR RE + RLG K A +A + L R P LD+RL L Sbjct: 140 GIYVRAVRLREMLRRRLGLPKMPAMVYALLAVLWRLPRLDLRLASL-------PTGFQSV 192 Query: 122 RTPFVFIGNNEYLMDGLNIGKRNSLSEGKLSLYVCHRTGRLGLVRLALHALFGRLRQARD 181 R PFVFIGNN Y L + + N L++G LS+ R L+ +A A ++Q Sbjct: 193 RVPFVFIGNNPYTSKPLPLVRENGLAQGCLSVLFTPGVSRKSLIMMATKAFLRPIQQDPA 252 Query: 182 FDMLSAAEIEIDTRHRHGQHRIKRLRVATDGEVTIMQTPLVYRIRPASLTVI 233 F+ E+ I + R RL VA DGEV +++PLV+R RP +L VI Sbjct: 253 FEERHVQELIIRSAKR-------RLYVALDGEVIALRSPLVFRTRPQALQVI 297