BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0412.p (371 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_004754722.1| hypothetical protein CFU_4077 [Collimonas fu... 127 4e-27 ref|YP_994675.1| 5-methylaminomethyl-2-thiouridine methyltransfe... 96 9e-18 sp|A1V8X2.2|MNMC_BURMS RecName: Full=tRNA 5-methylaminomethyl-2-... 96 1e-17 ref|ZP_01768259.1| oxidoreductase, FAD-binding [Burkholderia pse... 94 3e-17 ref|ZP_03788750.1| oxidoreductase, FAD-binding [Burkholderia pse... 94 3e-17 >ref|YP_004754722.1| hypothetical protein CFU_4077 [Collimonas fungivorans Ter331] gb|AEK63899.1| hypothetical protein CFU_4077 [Collimonas fungivorans Ter331] Length = 594 Score = 127 bits (318), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 93/247 (37%), Positives = 126/247 (51%), Gaps = 29/247 (11%) Query: 20 WRGRAVYTILQA--GYSDAFLQAWAALREQGDDVDTPQRLHYLLIDAALPTAQQLRAMAP 77 WRGR + +L + G + F AW A R D P++LHYL + L +L+ +A Sbjct: 15 WRGRDSFVLLDSEFGRGEHFFAAWQAWRS---DSRRPRQLHYLALLPQLAAHTELQQIAE 71 Query: 78 TN----ALAGELANVSPPLTPGYHRLLLEQGRVVLTLIIGEVAASLAQVDARVDLFLLQD 133 + AL EL PP PG+HR+ L+QG++VLTL+IG+ AA L Q+ A+VD F L Sbjct: 72 KHPEWQALCTELCAAWPPAVPGFHRIFLQQGQLVLTLMIGDGAACLRQIVAKVDAFCLHG 131 Query: 134 AAM--WNAPQLKRLARLATPRAQLRMGGAWRASSAELAAAGFTMDEDDKLTAHFTPRWKV 191 W+ P L L RLA A L + GA A+ +L AGF D D+ +A F PR+ Sbjct: 132 NRWREWSLPVLGTLGRLAAGHASLNIAGAEAAARKQLEQAGFLFDSSDERSARFNPRFTA 191 Query: 192 AERAVP--QDRRALVIGAGLXPPSRLAGDADRAACARRAGSLRQPCRRPYAGDLTRRQSD 249 A P DRRA+VIGAGL A AAC + +R ++ DL + Sbjct: 192 RSAAAPVHHDRRAIVIGAGL---------AGSAAC-------QHLVQRGWSVDLVESHTA 235 Query: 250 RAPDASG 256 A ASG Sbjct: 236 PAQQASG 242 >ref|YP_994675.1| 5-methylaminomethyl-2-thiouridine methyltransferase [Burkholderia mallei SAVP1] ref|ZP_02267099.2| oxidoreductase, FAD-binding [Burkholderia mallei PRL-20] gb|ABM50193.1| oxidoreductase, FAD-binding [Burkholderia mallei SAVP1] gb|EES45102.1| oxidoreductase, FAD-binding [Burkholderia mallei PRL-20] Length = 708 Score = 95.9 bits (237), Expect = 9e-18, Method: Compositional matrix adjust. Identities = 75/220 (34%), Positives = 99/220 (45%), Gaps = 33/220 (15%) Query: 20 WRGRAVYTILQAGYSDA--FLQAWAALREQGDDVDTPQRLHYLLIDAALPTAQQLR---- 73 WR +TI++ G+ FL WAA R D +RLH++ ++ + LR Sbjct: 102 WRHERAFTIIETGFGTGCNFLATWAAWRA---DPSHCERLHFVSVEKHPFAREDLRRAAA 158 Query: 74 ------AMAPTNALAGELANVSPPLTPGYHRLLLEQGRVVLTLIIGEVAASLAQVDARVD 127 + P L ELAN P LTPG HRL + GRV LTL+ G+ L + R Sbjct: 159 HIVAYTTITPIAPLVDELANAWPALTPGVHRLEFDDGRVTLTLVFGDALDVLPNLALRAH 218 Query: 128 LFLL------QDAAMWNAPQLKRLARLATPRAQLRMGGAWRASSAELAAAGFTMDEDD-- 179 F L ++A +W+ K LA+LA RA + A L AGF + D Sbjct: 219 AFYLDGFAPSKNADLWSPAIFKSLAKLADERATFATYTSSGAVKRALDEAGFAYRKVDGF 278 Query: 180 -----KLTAHFTPRWKV----AERAVPQDRR-ALVIGAGL 209 L F PRW+V RA DRR A+VIGAGL Sbjct: 279 AGKRAMLVGEFAPRWRVRRHEPPRAFSTDRRDAIVIGAGL 318 >sp|A1V8X2.2|MNMC_BURMS RecName: Full=tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein mnmC; Short=tRNA mnm(5)s(2)U biosynthesis bifunctional protein; Includes: RecName: Full=tRNA (mnm(5)s(2)U34)-methyltransferase; Includes: RecName: Full=FAD-dependent cmnm(5)s(2)U34 oxidoreductase Length = 657 Score = 95.5 bits (236), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 75/220 (34%), Positives = 99/220 (45%), Gaps = 33/220 (15%) Query: 20 WRGRAVYTILQAGYSDA--FLQAWAALREQGDDVDTPQRLHYLLIDAALPTAQQLR---- 73 WR +TI++ G+ FL WAA R D +RLH++ ++ + LR Sbjct: 51 WRHERAFTIIETGFGTGCNFLATWAAWRA---DPSHCERLHFVSVEKHPFAREDLRRAAA 107 Query: 74 ------AMAPTNALAGELANVSPPLTPGYHRLLLEQGRVVLTLIIGEVAASLAQVDARVD 127 + P L ELAN P LTPG HRL + GRV LTL+ G+ L + R Sbjct: 108 HIVAYTTITPIAPLVDELANAWPALTPGVHRLEFDDGRVTLTLVFGDALDVLPNLALRAH 167 Query: 128 LFLL------QDAAMWNAPQLKRLARLATPRAQLRMGGAWRASSAELAAAGFTMDEDD-- 179 F L ++A +W+ K LA+LA RA + A L AGF + D Sbjct: 168 AFYLDGFAPSKNADLWSPAIFKSLAKLADERATFATYTSSGAVKRALDEAGFAYRKVDGF 227 Query: 180 -----KLTAHFTPRWKV----AERAVPQDRR-ALVIGAGL 209 L F PRW+V RA DRR A+VIGAGL Sbjct: 228 AGKRAMLVGEFAPRWRVRRHEPPRAFSTDRRDAIVIGAGL 267 >ref|ZP_01768259.1| oxidoreductase, FAD-binding [Burkholderia pseudomallei 305] gb|EBA47304.1| oxidoreductase, FAD-binding [Burkholderia pseudomallei 305] Length = 734 Score = 94.4 bits (233), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 76/223 (34%), Positives = 100/223 (44%), Gaps = 36/223 (16%) Query: 20 WRGRAVYTILQAGYSDA--FLQAWAALREQGDDVDTPQRLHYLLIDAALPTAQQLRAMA- 76 WR +TI++ G+ FL WAA R D +RLH++ ++ + LR A Sbjct: 125 WRHERAFTIIETGFGTGCNFLATWAAWRA---DPSHCERLHFVSVEKHPFAREDLRRAAA 181 Query: 77 ------------PTNALAGELANVSPPLTPGYHRLLLEQGRVVLTLIIGEVAASLAQVDA 124 P L ELAN P LTPG HRL + GRV LTL+ G+ L + Sbjct: 182 HIVAYTTITTITPIAPLVDELANAWPALTPGVHRLEFDDGRVTLTLVFGDALDVLPNLAL 241 Query: 125 RVDLFLL------QDAAMWNAPQLKRLARLATPRAQLRMGGAWRASSAELAAAGFTMDED 178 R F L ++A +W+ K LA+LA RA + A L AGF + Sbjct: 242 RAHAFYLDGFAPSKNADLWSPAIFKSLAKLADERATFATYTSSGAVKRALDEAGFAYRKV 301 Query: 179 D-------KLTAHFTPRWKV----AERAVPQDRR-ALVIGAGL 209 D L F PRW+V RA+ DRR A+VIGAGL Sbjct: 302 DGFAGKRAMLVGEFAPRWRVRRHEPPRALSTDRRDAIVIGAGL 344 >ref|ZP_03788750.1| oxidoreductase, FAD-binding [Burkholderia pseudomallei Pakistan 9] gb|EEH30717.1| oxidoreductase, FAD-binding [Burkholderia pseudomallei Pakistan 9] Length = 711 Score = 94.4 bits (233), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 76/223 (34%), Positives = 100/223 (44%), Gaps = 36/223 (16%) Query: 20 WRGRAVYTILQAGYSDA--FLQAWAALREQGDDVDTPQRLHYLLIDAALPTAQQLRAMA- 76 WR +TI++ G+ FL WAA R D +RLH++ ++ + LR A Sbjct: 102 WRHERAFTIIETGFGTGCNFLATWAAWRA---DPSHCERLHFVSVEKHPFAREDLRRAAA 158 Query: 77 ------------PTNALAGELANVSPPLTPGYHRLLLEQGRVVLTLIIGEVAASLAQVDA 124 P L ELAN P LTPG HRL + GRV LTL+ G+ L + Sbjct: 159 HIVAYTTITTITPIAPLVDELANAWPALTPGVHRLEFDDGRVTLTLVFGDALDVLPNLAL 218 Query: 125 RVDLFLL------QDAAMWNAPQLKRLARLATPRAQLRMGGAWRASSAELAAAGFTMDED 178 R F L ++A +W+ K LA+LA RA + A L AGF + Sbjct: 219 RAHAFYLDGFAPSKNADLWSPAIFKSLAKLADERATFATYTSSGAVKRALDEAGFAYRKV 278 Query: 179 D-------KLTAHFTPRWKV----AERAVPQDRR-ALVIGAGL 209 D L F PRW+V RA+ DRR A+VIGAGL Sbjct: 279 DGFAGKRAMLVGEFAPRWRVRRHEPPRALSTDRRDAIVIGAGL 321