BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0205.p (332 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_004349860.1| D-3-phosphoglycerate dehydrogenase, putative... 345 9e-93 ref|YP_004155426.1| D-isomer specific 2-hydroxyacid dehydrogenas... 342 6e-92 ref|YP_001632430.1| D-3-phosphoglycerate dehydrogenase [Bordetel... 338 5e-91 ref|YP_551353.1| D-isomer specific 2-hydroxyacid dehydrogenase [... 338 5e-91 ref|ZP_06896209.1| D-isomer specific 2-hydroxyacid dehydrogenase... 327 1e-87 >ref|YP_004349860.1| D-3-phosphoglycerate dehydrogenase, putative [Burkholderia gladioli BSR3] gb|AEA64348.1| D-3-phosphoglycerate dehydrogenase, putative [Burkholderia gladioli BSR3] Length = 338 Score = 345 bits (884), Expect = 9e-93, Method: Compositional matrix adjust. Identities = 180/327 (55%), Positives = 230/327 (70%) Query: 1 MADIYLAYSPETRANYYGDKPLALLQALGTVRLNDSETPLTPSDLVRAAAGCDIIVAPRI 60 M +++++ +Y+G A L A+ R N + L+ ++L+ AA DII+ R Sbjct: 1 MTQVFVSHPRSMLDHYFGAHATAALGAIAEARFNPEDRELSTAELIEAARDADIIIGYRQ 60 Query: 61 PAATAEVFDHLPQLVAYCRGAVDIRNIDVAAASRNGVLVTRATPGFAASVAEWVLAAMFD 120 A +F+ LP L+A+ R AVDIR IDVAAAS +GVL+T+A+ GF A+V+EWV+ AM D Sbjct: 61 TPAPRALFEALPALLAFARCAVDIRTIDVAAASEHGVLITQASAGFMAAVSEWVIGAMLD 120 Query: 121 LSRGISDAALAYRNGIVPAVRMGRELRGATLGVAGYGTIGQYLCRIAQALGMKIVVADPY 180 LSR +D A AYR G P RMGRELRGATLGV GYG I +YLC +A A GM++VVADP+ Sbjct: 121 LSRSTTDYAAAYRRGERPVPRMGRELRGATLGVIGYGQISRYLCELALAFGMRVVVADPF 180 Query: 181 ASIIEPSHVAASFEAMLAQSDFVVCLAPATAETAQLFNAAAFAKMQASAFFINASRGELV 240 A++ + A EA+L ++D+VVCLAPA A+TAQL NA FA M+ A F+NA+RGELV Sbjct: 181 ATVTDLRMHQAGLEALLGEADYVVCLAPANADTAQLMNAERFAAMKPGAVFVNAARGELV 240 Query: 241 DEAALLQALDHGVILAAALDVGMAPDQMPSPLLARHPRVIATPHIGGLTPQAAEHQAMDT 300 DEAALL ALD G + ALDVGMA DQMPS LA HP VIATPH+GGLTP A EHQ+++T Sbjct: 241 DEAALLAALDGGRLGGCALDVGMAADQMPSARLAAHPLVIATPHVGGLTPGAIEHQSLET 300 Query: 301 VHQVAAILAGRLPQGAVNADQSHRLAR 327 V QV A+L GR+P GAVNA + RLAR Sbjct: 301 VAQVEALLQGRIPAGAVNAAHATRLAR 327 >ref|YP_004155426.1| D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding protein [Variovorax paradoxus EPS] gb|ADU37315.1| D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding protein [Variovorax paradoxus EPS] Length = 330 Score = 342 bits (877), Expect = 6e-92, Method: Compositional matrix adjust. Identities = 185/329 (56%), Positives = 231/329 (70%), Gaps = 4/329 (1%) Query: 1 MADIYLAYSPETRANYYGDKPLALLQALGTVRLNDSETPLTPSDLVRAAAGCDIIVAPRI 60 M +++++ A Y+GD+ +A LQA+ VR N ++ L+ +L A GCD I++ R Sbjct: 1 MTTVFVSHPQSKLAPYFGDRAIAALQAIAQVRFNPTDHELSSGELAGLAQGCDAIISYRQ 60 Query: 61 PAATAEVFDHLPQLVAYCRGAVDIRNIDVAAASRNGVLVTRATPGFAASVAEWVLAAMFD 120 +F LP L A+ R A+DIRNIDVA+ASR+GVLVT+A+ GF ASV+EW++ AM D Sbjct: 61 TVGDEALFAALPDLKAFVRCAIDIRNIDVASASRHGVLVTQASAGFIASVSEWIVGAMID 120 Query: 121 LSRGISDAALAYRNG--IVPAVRMGRELRGATLGVAGYGTIGQYLCRIAQALGMKIVVAD 178 L R IS + Y G +VPA MGRELRG+TLGV GYG I +YLC +A ALGM IVV D Sbjct: 121 LGRHISASTALYHAGQPVVPA--MGRELRGSTLGVIGYGQISRYLCDVALALGMHIVVYD 178 Query: 179 PYASIIEPSHVAASFEAMLAQSDFVVCLAPATAETAQLFNAAAFAKMQASAFFINASRGE 238 P+ASI P + +A+LA+ DFVVCLAPAT T L NAAAFA M+ AFFINASRG+ Sbjct: 179 PHASIERPGLEQVALDALLARCDFVVCLAPATEATENLMNAAAFAAMRPQAFFINASRGD 238 Query: 239 LVDEAALLQALDHGVILAAALDVGMAPDQMPSPLLARHPRVIATPHIGGLTPQAAEHQAM 298 LVDE ALL ALD G I ALDVG APDQMPSP +A HPRVIATPHIGGLTP A EHQAM Sbjct: 239 LVDEDALLTALDAGTIAGCALDVGRAPDQMPSPRVAAHPRVIATPHIGGLTPPAVEHQAM 298 Query: 299 DTVHQVAAILAGRLPQGAVNADQSHRLAR 327 +TV Q+ + GR+P+GAVNA ++ R + Sbjct: 299 ETVGQLGELFQGRMPKGAVNAAEAWRWRK 327 >ref|YP_001632430.1| D-3-phosphoglycerate dehydrogenase [Bordetella petrii DSM 12804] emb|CAP44162.1| D-3-phosphoglycerate dehydrogenase [Bordetella petrii] Length = 337 Score = 338 bits (868), Expect = 5e-91, Method: Compositional matrix adjust. Identities = 184/325 (56%), Positives = 229/325 (70%), Gaps = 1/325 (0%) Query: 1 MADIYLAYSPETRANYYGDKPLALLQALGTVRLNDSETPLTPSDLVRAAAGCDIIVAPRI 60 M I+L ++ +YYG+ +L + V +DS++ T D+VR A CDI+V+ RI Sbjct: 1 MTRIFLTHNRAALRHYYGEPAYRMLAGMAQVAFHDSDSEPTQDDIVRQARDCDILVSFRI 60 Query: 61 PAATAEVFDHLPQLVAYCRGAVDIRNIDVAAASRNGVLVTRATPGFAASVAEWVLAAMFD 120 P A + LP+L A CR AVDIRNIDV ASR+G+LV RATPGF ASVAEWV+ AM Sbjct: 61 PPIDARLLAALPRLAAVCRVAVDIRNIDVDYASRHGILVMRATPGFGASVAEWVVGAMVM 120 Query: 121 LSRGISDAALAYRNGIVPAVRMGRELRGATLGVAGYGTIGQYLCRIAQALGMKIVVADPY 180 L+R + A AY+NG P MG ELR ATLG+ GYGTIG+YL +A+A GM++V+ DP+ Sbjct: 121 LARHMGPATCAYQNGQRPVPLMGTELRAATLGIIGYGTIGRYLAGLARAFGMRLVINDPH 180 Query: 181 ASI-IEPSHVAASFEAMLAQSDFVVCLAPATAETAQLFNAAAFAKMQASAFFINASRGEL 239 + EP A+ + +LAQSDFVVCLAPA AETA+L N AF KM+ SA+FINASR EL Sbjct: 181 VRVDTEPGVTQATLDELLAQSDFVVCLAPAIAETARLINLPAFRKMKPSAYFINASRAEL 240 Query: 240 VDEAALLQALDHGVILAAALDVGMAPDQMPSPLLARHPRVIATPHIGGLTPQAAEHQAMD 299 VDE LLQALD G+I ALDVG A DQMP LA HPRV+ATPHIGGLTPQA+EHQAMD Sbjct: 241 VDEDDLLQALDAGLIAGCALDVGSAQDQMPPARLAAHPRVVATPHIGGLTPQASEHQAMD 300 Query: 300 TVHQVAAILAGRLPQGAVNADQSHR 324 +V QV A+L GR+P+ +VNA + R Sbjct: 301 SVRQVRALLEGRMPECSVNAAHATR 325 >ref|YP_551353.1| D-isomer specific 2-hydroxyacid dehydrogenase [Polaromonas sp. JS666] gb|ABE46455.1| D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding protein [Polaromonas sp. JS666] Length = 335 Score = 338 bits (868), Expect = 5e-91, Method: Compositional matrix adjust. Identities = 180/324 (55%), Positives = 223/324 (68%) Query: 1 MADIYLAYSPETRANYYGDKPLALLQALGTVRLNDSETPLTPSDLVRAAAGCDIIVAPRI 60 M +++++ + +Y+G + A LQAL VR N +T L+ +L A CD+I++ R Sbjct: 1 MTTVFVSHPRTSLGHYFGARATAALQALADVRFNPGDTDLSSRELAALAQDCDVIISYRQ 60 Query: 61 PAATAEVFDHLPQLVAYCRGAVDIRNIDVAAASRNGVLVTRATPGFAASVAEWVLAAMFD 120 +F LP+L A+ R A+DIRNIDV AASR+GVLVT+A+ GF ASV+EW++ M D Sbjct: 61 TQGDEALFAALPRLKAFVRCAIDIRNIDVPAASRHGVLVTQASAGFIASVSEWIIGVMID 120 Query: 121 LSRGISDAALAYRNGIVPAVRMGRELRGATLGVAGYGTIGQYLCRIAQALGMKIVVADPY 180 LSR IS +A Y G A MGRELRGATLGV GYG I +YLC +A ALGM+IVV DPY Sbjct: 121 LSRHISASAALYHAGRPVAPLMGRELRGATLGVIGYGQISRYLCDVALALGMRIVVHDPY 180 Query: 181 ASIIEPSHVAASFEAMLAQSDFVVCLAPATAETAQLFNAAAFAKMQASAFFINASRGELV 240 V +LA++D+VVCLA AT T L NA AFA M+ AFFINASRG LV Sbjct: 181 TRTGRQELVQTGLAPLLAEADYVVCLAAATEATENLMNAQAFALMKPGAFFINASRGNLV 240 Query: 241 DEAALLQALDHGVILAAALDVGMAPDQMPSPLLARHPRVIATPHIGGLTPQAAEHQAMDT 300 DE+ALL ALD G + A+DVG APDQMPSP +A HPRVIA+PHIGGLTP A EHQA++T Sbjct: 241 DESALLAALDAGTMAGCAVDVGRAPDQMPSPRVAAHPRVIASPHIGGLTPPAVEHQALET 300 Query: 301 VHQVAAILAGRLPQGAVNADQSHR 324 V QVA IL G +PQGAVNA Q+HR Sbjct: 301 VAQVADILQGCVPQGAVNAAQAHR 324 >ref|ZP_06896209.1| D-isomer specific 2-hydroxyacid dehydrogenase [Roseomonas cervicalis ATCC 49957] gb|EFH12087.1| D-isomer specific 2-hydroxyacid dehydrogenase [Roseomonas cervicalis ATCC 49957] Length = 347 Score = 327 bits (839), Expect = 1e-87, Method: Compositional matrix adjust. Identities = 190/328 (57%), Positives = 222/328 (67%), Gaps = 2/328 (0%) Query: 4 IYLAYSPETRANYYGDKPLALLQALGTVRLNDSETPLTPSDLVRAAAGCDIIVAPRIPAA 63 I L ++P R YYG+ L L G +RLN+S+ PL LV A GCDIIVA R+ AA Sbjct: 16 ILLTHAPRARDLYYGEAALRALAEQGELRLNESDAPLDTEALVALAEGCDIIVADRLAAA 75 Query: 64 TAEVFDHLPQLVAYCRGAVDIRNIDVAAASRNGVLVTRATPGFAASVAEWVLAAMFDLSR 123 A +F LP L A R AVDIRNIDV AAS+ GVLV RATPGF SVAE L M DLSR Sbjct: 76 PAALFGRLPDLAALLRVAVDIRNIDVEAASQAGVLVCRATPGFVDSVAELALGLMLDLSR 135 Query: 124 GISDAALAYRNGIVPAVRMGRELRGATLGVAGYGTIGQYLCRIAQALGMKIVVADPY--A 181 GISDA LAYR G P VRMGR+L GATLG+ G+G IG+ L R+A +LGM ++ +DP Sbjct: 136 GISDAVLAYRAGQAPPVRMGRQLSGATLGIIGHGAIGRKLGRLAASLGMTVLASDPALPE 195 Query: 182 SIIEPSHVAASFEAMLAQSDFVVCLAPATAETAQLFNAAAFAKMQASAFFINASRGELVD 241 ASF +LA SD VVCLAPA ET + N AAFA M+ A F+N +RGELVD Sbjct: 196 GAEREGARGASFATLLAASDIVVCLAPARPETEGMMNGAAFAAMRRGALFLNLARGELVD 255 Query: 242 EAALLQALDHGVILAAALDVGMAPDQMPSPLLARHPRVIATPHIGGLTPQAAEHQAMDTV 301 EAAL AL+ G + AALDVG A DQMP+P LA P V+ATPHIGGLTPQAA+HQA DTV Sbjct: 256 EAALAAALESGQLGGAALDVGRAADQMPNPSLAARPDVVATPHIGGLTPQAAQHQAFDTV 315 Query: 302 HQVAAILAGRLPQGAVNADQSHRLARFS 329 QVAA+ AGR+P+ AVN + RLAR Sbjct: 316 RQVAALAAGRMPEHAVNPAAAQRLARLG 343