BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0070.p (330 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_298346.1| putative citrate lyase [Ralstonia eutropha JMP1... 443 e-122 ref|YP_003980983.1| citrate lyase subunit beta-like protein [Ach... 441 e-122 ref|YP_586563.1| Citrate lyase subunit beta [Cupriavidus metalli... 436 e-120 ref|YP_728518.1| citrate lyase beta subunit [Ralstonia eutropha ... 434 e-120 pdb|3QQW|A Chain A, Crystal Structure Of A Hypothetical Lyase (R... 427 e-117 >ref|YP_298346.1| putative citrate lyase [Ralstonia eutropha JMP134] gb|AAZ63502.1| putative citrate lyase [Ralstonia eutropha JMP134] Length = 331 Score = 443 bits (1139), Expect = e-122, Method: Compositional matrix adjust. Identities = 210/329 (63%), Positives = 249/329 (75%) Query: 1 MPIQVHPEDALFMGEKTFPPLPVCEHFAGNEKLIRKALQIQERLGPVFDITCDCEDGAQA 60 M HP DALF GEK+FP L CEHFAG+EKLI KA+ +Q GPVFD+TCDCEDGA A Sbjct: 1 MTTATHPNDALFAGEKSFPVLAACEHFAGSEKLIGKAMDLQVEYGPVFDVTCDCEDGAAA 60 Query: 61 GDEIGHASMVAELVRTASNSDGRIGVRIHDRSHASWRSDVDIVVGRAASELAYVTIPKST 120 G E HA MVA ++ + N GR G RIHD SH +WR DVDI+V A LAY+T+PK+T Sbjct: 61 GQEREHAEMVARMIASDRNVHGRAGARIHDPSHPAWRQDVDIIVNGAGGRLAYITVPKAT 120 Query: 121 SVAGARECIDYIQQTARSAGRATPLPVHILVETHAALRHVYALAELPHVEVLDFGLMDFV 180 + E I YI A+ AG P+PVH+L+ETH ALR V+ +AELP++EVLDFGLMDFV Sbjct: 121 NSGQVAEVIRYIGDVAKRAGLDKPVPVHVLIETHGALRDVFQIAELPNIEVLDFGLMDFV 180 Query: 181 SSHHGAIGADALRSPGQFEHALLTRAKADVVAAALAAGVVPSHNVCLNLRDAAVIRGDAL 240 S HHGAI A A+RSPGQFEHALL RAKAD+VAAALA G+VP+HNVCLNL+DA VI DA Sbjct: 181 SGHHGAIPAAAMRSPGQFEHALLVRAKADMVAAALANGIVPAHNVCLNLKDAEVIASDAC 240 Query: 241 RASRQFGFQRMWSIHPSQIEPIVAALRPDMNEVEDASAILVAAQDNQWGPIQFKGELHDR 300 RA +FGF RMWSI+P+QI+PIV A+RPD EVEDA+ ILVAAQD WGPIQ+KGELHDR Sbjct: 241 RARNEFGFLRMWSIYPAQIQPIVNAMRPDFTEVEDAAGILVAAQDADWGPIQYKGELHDR 300 Query: 301 ATYRYFWSLLKRGHSTCAPLPAIVVQRFF 329 ATYRYFW +L++ T +PA +RFF Sbjct: 301 ATYRYFWEVLQKAKVTGMAVPAEAERRFF 329 >ref|YP_003980983.1| citrate lyase subunit beta-like protein [Achromobacter xylosoxidans A8] gb|ADP18268.1| citrate lyase beta subunit-like protein [Achromobacter xylosoxidans A8] Length = 331 Score = 441 bits (1134), Expect = e-122, Method: Compositional matrix adjust. Identities = 213/330 (64%), Positives = 251/330 (76%) Query: 1 MPIQVHPEDALFMGEKTFPPLPVCEHFAGNEKLIRKALQIQERLGPVFDITCDCEDGAQA 60 M +HP DALF GE FP LPVCEHFAG+EKLI KA+ +Q GPVFDITCDCEDGA A Sbjct: 1 MTTAIHPRDALFAGESPFPVLPVCEHFAGSEKLIGKAMDLQAEYGPVFDITCDCEDGAAA 60 Query: 61 GDEIGHASMVAELVRTASNSDGRIGVRIHDRSHASWRSDVDIVVGRAASELAYVTIPKST 120 G E HA MVA ++ +A N R G RIHD SH +WR DVDI+V +A +AY+TIPK+T Sbjct: 61 GQERVHAEMVAGMINSARNKFDRAGARIHDPSHPAWRQDVDIIVSQAGGRVAYITIPKAT 120 Query: 121 SVAGARECIDYIQQTARSAGRATPLPVHILVETHAALRHVYALAELPHVEVLDFGLMDFV 180 S A E I YI +TAR AG +P H+LVETH ALR + +A LP VEVLDFGLMDFV Sbjct: 121 SWQQAAEVIGYIGETARKAGLERQIPAHVLVETHGALRDAFPIAALPTVEVLDFGLMDFV 180 Query: 181 SSHHGAIGADALRSPGQFEHALLTRAKADVVAAALAAGVVPSHNVCLNLRDAAVIRGDAL 240 S HHGAI A A+RSPGQFEHALL RAKA+VVAAALA GVVP+HNVCLNL+D +I DA Sbjct: 181 SGHHGAIAASAMRSPGQFEHALLVRAKAEVVAAALAHGVVPAHNVCLNLKDPGIIADDAG 240 Query: 241 RASRQFGFQRMWSIHPSQIEPIVAALRPDMNEVEDASAILVAAQDNQWGPIQFKGELHDR 300 RA RQFGF RMWSI+P+QI+PIV A++P+ +EVEDA+ IL+AAQDN WGPIQ++GELHDR Sbjct: 241 RARRQFGFLRMWSIYPAQIQPIVQAMQPEFSEVEDAANILLAAQDNDWGPIQYEGELHDR 300 Query: 301 ATYRYFWSLLKRGHSTCAPLPAIVVQRFFA 330 ATYRYFW +L++ T LP +RFFA Sbjct: 301 ATYRYFWEVLRKARITGMTLPPEAQRRFFA 330 >ref|YP_586563.1| Citrate lyase subunit beta [Cupriavidus metallidurans CH34] gb|ABF11294.1| Citrate lyase beta subunit-like protein [Cupriavidus metallidurans CH34] Length = 373 Score = 436 bits (1122), Expect = e-120, Method: Compositional matrix adjust. Identities = 209/330 (63%), Positives = 249/330 (75%) Query: 1 MPIQVHPEDALFMGEKTFPPLPVCEHFAGNEKLIRKALQIQERLGPVFDITCDCEDGAQA 60 M HP DALF GEK FP L CEHFAG+EKLI KA+ +Q GPVFD+TCDCEDGA Sbjct: 43 MTTATHPRDALFAGEKAFPALAACEHFAGSEKLIGKAMDLQAEYGPVFDVTCDCEDGAAT 102 Query: 61 GDEIGHASMVAELVRTASNSDGRIGVRIHDRSHASWRSDVDIVVGRAASELAYVTIPKST 120 G E HA MVA ++ + N GR G RIHD SH +WR DV+I+VG+A LAY+T+PK+T Sbjct: 103 GQEREHAEMVARMIASDRNRFGRAGARIHDPSHPAWRQDVEIIVGQAGGRLAYITVPKAT 162 Query: 121 SVAGARECIDYIQQTARSAGRATPLPVHILVETHAALRHVYALAELPHVEVLDFGLMDFV 180 E I +I++TA+ AG P+PVH+L+ETH ALR V+ +AELP +EVLDFGLMDFV Sbjct: 163 RHEQVAEVIAFIRETAQRAGLERPVPVHVLIETHGALRDVFPIAELPDIEVLDFGLMDFV 222 Query: 181 SSHHGAIGADALRSPGQFEHALLTRAKADVVAAALAAGVVPSHNVCLNLRDAAVIRGDAL 240 S HHGAI A A+RSPGQFEHALL RAKAD+VAAALA G+VP+HNVCLNL+DA VI DA Sbjct: 223 SGHHGAIPAAAMRSPGQFEHALLARAKADMVAAALANGIVPAHNVCLNLKDADVIGADAS 282 Query: 241 RASRQFGFQRMWSIHPSQIEPIVAALRPDMNEVEDASAILVAAQDNQWGPIQFKGELHDR 300 RA QFGF RMWSI+P+QI IV A+RPD EVEDA+ IL+AAQD WGPIQ+KGELHDR Sbjct: 283 RARNQFGFLRMWSIYPAQILSIVNAMRPDFAEVEDAAGILLAAQDADWGPIQYKGELHDR 342 Query: 301 ATYRYFWSLLKRGHSTCAPLPAIVVQRFFA 330 ATYRYFW +L++ T +PA +RFFA Sbjct: 343 ATYRYFWEVLEKARVTGMAMPAEAERRFFA 372 >ref|YP_728518.1| citrate lyase beta subunit [Ralstonia eutropha H16] emb|CAJ95153.1| citrate lyase beta subunit [Ralstonia eutropha H16] Length = 331 Score = 434 bits (1117), Expect = e-120, Method: Compositional matrix adjust. Identities = 208/329 (63%), Positives = 244/329 (74%) Query: 1 MPIQVHPEDALFMGEKTFPPLPVCEHFAGNEKLIRKALQIQERLGPVFDITCDCEDGAQA 60 M HP DALF GEK FP L CEHFAG+ KLI KA+ +Q G FD+TCDCEDGA A Sbjct: 1 MITPTHPNDALFAGEKAFPVLAACEHFAGSGKLIGKAMDLQVEYGAAFDVTCDCEDGAAA 60 Query: 61 GDEIGHASMVAELVRTASNSDGRIGVRIHDRSHASWRSDVDIVVGRAASELAYVTIPKST 120 G E HA MVA ++ + N GR G RIHD SH +WR DVDI+V A S LAY+T+PK+T Sbjct: 61 GQEREHAQMVARMIASERNRFGRAGARIHDPSHPAWRQDVDIIVREAGSRLAYITVPKAT 120 Query: 121 SVAGARECIDYIQQTARSAGRATPLPVHILVETHAALRHVYALAELPHVEVLDFGLMDFV 180 E I YI + G P+PVH+L+ETH ALR V+ +AELPH+EVLDFGLMDFV Sbjct: 121 GSGQVAEVIRYIDEVRTKTGLDKPVPVHVLIETHGALRDVFEIAELPHIEVLDFGLMDFV 180 Query: 181 SSHHGAIGADALRSPGQFEHALLTRAKADVVAAALAAGVVPSHNVCLNLRDAAVIRGDAL 240 S HHGAI A A+RSPGQFEHALL RAKAD+VAAALA G+VP+HNVCLNL+DA VI DA Sbjct: 181 SGHHGAIPAAAMRSPGQFEHALLGRAKADMVAAALANGIVPAHNVCLNLKDAEVIASDAR 240 Query: 241 RASRQFGFQRMWSIHPSQIEPIVAALRPDMNEVEDASAILVAAQDNQWGPIQFKGELHDR 300 RA +FGF RMWSI+P+QI+PIV A+RPD+ EVEDA+ IL AAQD WGPIQ+KGELHDR Sbjct: 241 RARDEFGFLRMWSIYPAQIQPIVNAMRPDLAEVEDAAGILSAAQDADWGPIQYKGELHDR 300 Query: 301 ATYRYFWSLLKRGHSTCAPLPAIVVQRFF 329 ATYRYFW +L++ T LPA V+RFF Sbjct: 301 ATYRYFWEILRKAKVTGMTLPAEAVRRFF 329 >pdb|3QQW|A Chain A, Crystal Structure Of A Hypothetical Lyase (Reut_b4148) From Ralstonia Eutropha Jmp134 At 2.44 A Resolution pdb|3QQW|B Chain B, Crystal Structure Of A Hypothetical Lyase (Reut_b4148) From Ralstonia Eutropha Jmp134 At 2.44 A Resolution pdb|3QQW|C Chain C, Crystal Structure Of A Hypothetical Lyase (Reut_b4148) From Ralstonia Eutropha Jmp134 At 2.44 A Resolution pdb|3QQW|D Chain D, Crystal Structure Of A Hypothetical Lyase (Reut_b4148) From Ralstonia Eutropha Jmp134 At 2.44 A Resolution pdb|3QQW|E Chain E, Crystal Structure Of A Hypothetical Lyase (Reut_b4148) From Ralstonia Eutropha Jmp134 At 2.44 A Resolution pdb|3QQW|F Chain F, Crystal Structure Of A Hypothetical Lyase (Reut_b4148) From Ralstonia Eutropha Jmp134 At 2.44 A Resolution Length = 332 Score = 427 bits (1097), Expect = e-117, Method: Compositional matrix adjust. Identities = 206/325 (63%), Positives = 240/325 (73%) Query: 5 VHPEDALFMGEKTFPPLPVCEHFAGNEKLIRKALQIQERLGPVFDITCDCEDGAQAGDEI 64 HP DALF GEK+FP L CEHFAG+EKLI KA +Q GPVFD+TCDCEDGA AG E Sbjct: 6 THPNDALFAGEKSFPVLAACEHFAGSEKLIGKAXDLQVEYGPVFDVTCDCEDGAAAGQER 65 Query: 65 GHASMVAELVRTASNSDGRIGVRIHDRSHASWRSDVDIVVGRAASELAYVTIPKSTSVAG 124 HA VA + + N GR G RIHD SH +WR DVDI+V A LAY+T+PK+T+ Sbjct: 66 EHAEXVARXIASDRNVHGRAGARIHDPSHPAWRQDVDIIVNGAGGRLAYITVPKATNSGQ 125 Query: 125 ARECIDYIQQTARSAGRATPLPVHILVETHAALRHVYALAELPHVEVLDFGLMDFVSSHH 184 E I YI A+ AG P+PVH+L+ETH ALR V+ +AELP++EVLDFGL DFVS HH Sbjct: 126 VAEVIRYIGDVAKRAGLDKPVPVHVLIETHGALRDVFQIAELPNIEVLDFGLXDFVSGHH 185 Query: 185 GAIGADALRSPGQFEHALLTRAKADVVAAALAAGVVPSHNVCLNLRDAAVIRGDALRASR 244 GAI A A RSPGQFEHALL RAKAD VAAALA G+VP+HNVCLNL+DA VI DA RA Sbjct: 186 GAIPAAAXRSPGQFEHALLVRAKADXVAAALANGIVPAHNVCLNLKDAEVIASDACRARN 245 Query: 245 QFGFQRMWSIHPSQIEPIVAALRPDMNEVEDASAILVAAQDNQWGPIQFKGELHDRATYR 304 +FGF R WSI+P+QI+PIV A RPD EVEDA+ ILVAAQD WGPIQ+KGELHDRATYR Sbjct: 246 EFGFLRXWSIYPAQIQPIVNAXRPDFTEVEDAAGILVAAQDADWGPIQYKGELHDRATYR 305 Query: 305 YFWSLLKRGHSTCAPLPAIVVQRFF 329 YFW +L++ T +PA +RFF Sbjct: 306 YFWEVLQKAKVTGXAVPAEAERRFF 330