BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0067.p (434 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_001264649.1| major facilitator transporter [Sphingomonas ... 242 8e-62 ref|YP_548890.1| major facilitator transporter [Polaromonas sp. ... 238 2e-60 ref|YP_004445483.1| major facilitator superfamily protein [Halis... 224 3e-56 ref|YP_003072834.1| transporter major facilitator family [Teredi... 223 7e-56 ref|YP_001475257.1| major facilitator transporter [Shewanella se... 221 1e-55 >ref|YP_001264649.1| major facilitator transporter [Sphingomonas wittichii RW1] gb|ABQ70511.1| major facilitator superfamily MFS_1 [Sphingomonas wittichii RW1] Length = 426 Score = 242 bits (618), Expect = 8e-62, Method: Compositional matrix adjust. Identities = 143/410 (34%), Positives = 225/410 (54%), Gaps = 16/410 (3%) Query: 12 SSPPRTTRAIHR-----GWWMVAISFLGLAFGQTSLAFLTLGVFMKPFNQEFGWTRGQVA 66 +S T+ I + GW ++ + + FG +L +T +F+ EFGWTR +++ Sbjct: 2 TSSQHDTKTIGKAPALFGWHIIFATVVAATFGPATLVNVTFSLFLPALQHEFGWTRSEIS 61 Query: 67 IALSVGAFVLLFTTPVVGRIIDRVGARKILLPSMAAFGVSMCSMYFLTGSLIHFYFMFAV 126 ++L++ VL+ PV+GRIID VG R+ L S+ A+ +++ + FL GSL Y +F + Sbjct: 62 LSLTIFIMVLVAMLPVMGRIIDAVGGRRSALVSIPAYAIALMGLGFLDGSLFTLYVLFGL 121 Query: 127 VGVVGAAANNVSYVRILSAWFVRHRGLSIGIASSGVAAGQSFGITLAQNVITDQGWRMAY 186 + + A +++ ++ILS WF R RGL+IG+ +G G A+ +I GWR AY Sbjct: 122 IAALSVGAQSLTLIKILSHWFDRRRGLAIGLCMAGNGLGYMLTPIFARVMIDGLGWRWAY 181 Query: 187 VALGASVLLVGLPIVALFIKNDPKDVG--LEPCGADDVAGEKISLGYYVSKAEALRMPVF 244 V LG L + P++ +++ P++ G ++ C AD + + G +S A+A R F Sbjct: 182 VGLGCLALFITFPVIYFLVRDTPQECGQTVDGCPADPRQPIQATAG--LSLAQAARTRSF 239 Query: 245 WALIFLAFFMATALHGIQIHFVPLLLDAGLSPGQVTGLLVSVLSVGAIVGRVGTGFLFDR 304 W L + AL+G+Q VPLLLD GLS T +LV+V +G+ GRV G+L DR Sbjct: 240 WLLAITFVLCSFALNGLQSQIVPLLLDRGLSSDMATLILVAV-GMGSFPGRVIAGYLMDR 298 Query: 305 FFAPYVAIGVFGLPIIGLLLVLNHVGLWGLCAGAFFFGIGLGSESDVLGYFTSRYFGLKS 364 FFAPYV G + L ++G+L + N + L A G+ LG+E+D LGY T RYFGL+ Sbjct: 299 FFAPYVVSGFYALSVVGILTLANSSAMAPLFLSAIVLGLSLGAENDALGYMTGRYFGLRK 358 Query: 365 MAELYGYIFGAFMAGSSAGPALYGIVQGKTGNYDTTLFASIGMLMFVCLV 414 ++Y ++ G++ GP + TG+Y T GML+ LV Sbjct: 359 FGQIYSICLCFYLIGAATGPYFMALSFDHTGSYRT------GMLLGTVLV 402 >ref|YP_548890.1| major facilitator transporter [Polaromonas sp. JS666] gb|ABE43992.1| major facilitator superfamily MFS_1 [Polaromonas sp. JS666] Length = 414 Score = 238 bits (606), Expect = 2e-60, Method: Compositional matrix adjust. Identities = 141/411 (34%), Positives = 221/411 (53%), Gaps = 10/411 (2%) Query: 21 IHRGWWMVAISFLGLAFGQTSLAFLTLGVFMKPFNQEFGWTRGQVAIALSVGAFVLLFTT 80 ++ WW+V S GL G + T GVF+ P +EFGW+R + AL +G + Sbjct: 3 LNNRWWIVVGSVFGLLVGNGPIMQFTFGVFLLPLTKEFGWSRSTTSFALVIGLTMTALFV 62 Query: 81 PVVGRIIDRVGARKILLPSMAAFGVSMCSMYFLTGSLIHFYFMFAVVGVVGAAANNVSYV 140 PV GR+IDR G R + LP++ F +SM ++ + F ++A++G+ A + Y Sbjct: 63 PVAGRLIDRFGIRAVTLPAITLFSLSMAALAVFADTPSKFMLIYALMGIAAAGQTPLPYA 122 Query: 141 RILSAWFVRHRGLSIGIASSGVAAGQSFGITLAQNVITDQGWRMAYVALGASVLLVGLPI 200 + ++AWF RGL++G+A SGV G + AQ ++ + GW+ AY+ L A +VG+ Sbjct: 123 KAIAAWFDDKRGLALGVAMSGVGLGAALVPQFAQMLVANVGWKGAYLGLAALTFVVGVCA 182 Query: 201 VALFIKNDPKDVGLEPCGADDVAGEKISLGYYVSKAEALRMPVFWALIFLAFFMATALHG 260 V L ++ KD GA ++ G ++ +ALR VFW L +A A +G Sbjct: 183 VGLAVREPGKD-HRSQAGAGNLPG--------LTGRQALRSSVFWKLAISFAAVAFATNG 233 Query: 261 IQIHFVPLLLDAGLSPGQVTGLLVSVLSVGAIVGRVGTGFLFDRFFAPYVAIGVFGLPII 320 + H VP+L+D G+ P T L + I GR+ GFL DR FAP+VA F +P+I Sbjct: 234 VIAHVVPMLVDRGVGPSVATTAL-GFAGLALIGGRLLAGFLLDRIFAPHVAAFFFAIPLI 292 Query: 321 GLLLVLNHVGLWGLCAGAFFFGIGLGSESDVLGYFTSRYFGLKSMAELYGYIFGAFMAGS 380 G+ ++ ++V G+GLG+E D++ + SRY GL+S E+YGY+F AFM GS Sbjct: 293 GIGILFSNVAPGYAAVAVVMVGLGLGAEVDLIAFLLSRYLGLRSFGEIYGYLFAAFMLGS 352 Query: 381 SAGPALYGIVQGKTGNYDTTLFASIGMLMFVCLVCAFLPRFKDKNNQAIAD 431 AGP + G V TG+Y L G+L+ CL+ L ++ +++ + Sbjct: 353 GAGPYMMGFVFDATGSYTAALAGLSGVLVIACLLLLSLGKYMYPQKESLNN 403 >ref|YP_004445483.1| major facilitator superfamily protein [Haliscomenobacter hydrossis DSM 1100] gb|AEE48610.1| major facilitator superfamily MFS_1 [Haliscomenobacter hydrossis DSM 1100] Length = 402 Score = 224 bits (570), Expect = 3e-56, Method: Compositional matrix adjust. Identities = 128/382 (33%), Positives = 218/382 (57%), Gaps = 8/382 (2%) Query: 19 RAIHRGWWMVAISFLGLAFGQTSLAFLTLGVFMKPFNQEFGWTRGQVAIALSVGAFVLLF 78 + I GW +V ++ +G SL GVF+KP + F RG +++A+S+ + + Sbjct: 3 QTIFYGWRIVLVALIGQFLSVGSLLVYCFGVFVKPLAEAFKTDRGSISLAVSLVSVGVAL 62 Query: 79 TTPVVGRIIDRVGARKILLPSMAAFGVSMC--SMYFLTGSLIHFYFMFAVVGVVGAAANN 136 +P+ GR+ID++G +K++ + A G+S+C ++ + G +I Y + + G +G+ + Sbjct: 63 GSPIAGRLIDKLGGKKVI--TYALIGMSLCLFALSRVNGPIISLYIFYFLGGFIGSGNSP 120 Query: 137 VSYVRILSAWFVRHRGLSIGIASSGVAAGQSFGITLAQNVITDQGWRMAYVALGASVLLV 196 ++Y RI++ WF +HR L+IG+ ++GV G L Q +I WR Y+ L + +L+ Sbjct: 121 LAYSRIVANWFNQHRALAIGLGNAGVGLGALLIPILGQYLIQSADWRQVYIILSLACVLI 180 Query: 197 GLPIVALFIKNDPKDVGLEPCGADDVAGEKISLGYYVSKAEALRMPVFWALIFLAFFMAT 256 LPIV+LF+KN P+++GL+P G D++ + S +S A FW L +A Sbjct: 181 ALPIVSLFLKNTPEELGLKPLGIIDISSQPSSA---ISIGAAAATRTFWLLSLSILCVAI 237 Query: 257 ALHGIQIHFVPLLLDAGLSPGQVTGLLVSVLSVGAIVGRVGTGFLFDRFFAPYVAIGVFG 316 + GI H +L D GLSP Q+ +S+ +++GR+ GFL DRF VA G+F Sbjct: 238 SCTGIMTHLAAMLTDRGLSP-QIAAFAISLFGGASLLGRIANGFLADRFHPSLVAAGIFS 296 Query: 317 LPIIGLLLVLNHVGLWGLCAGAFFFGIGLGSESDVLGYFTSRYFGLKSMAELYGYIFGAF 376 IG+LL+ + + + G+ +G+ESD++ Y SRYFG++SM +YG++F A+ Sbjct: 297 GAAIGILLLWLYPTGFTVYLATVMIGLAMGAESDIMPYMVSRYFGMRSMGTVYGFVFSAY 356 Query: 377 MAGSSAGPALYGIVQGKTGNYD 398 G++ GP L+GI +TG+Y Sbjct: 357 TVGAALGPLLFGIGFDQTGSYQ 378 >ref|YP_003072834.1| transporter major facilitator family [Teredinibacter turnerae T7901] gb|ACR11033.1| transporter, major facilitator family [Teredinibacter turnerae T7901] Length = 419 Score = 223 bits (567), Expect = 7e-56, Method: Compositional matrix adjust. Identities = 142/423 (33%), Positives = 226/423 (53%), Gaps = 22/423 (5%) Query: 14 PPRTTRAIHRG---WWMVAISFLGLAFGQTSLAFLTLGVFMKPFNQEFGWTRGQVAIALS 70 PP A RG W+++ I LG++ G + ++G+F +P ++EFGW R +++A+S Sbjct: 2 PPAQDHA--RGAHAWFIIVICLLGISSGPAAFCLASIGLFSEPLSREFGWDRTGISLAVS 59 Query: 71 VGAFVLLFTTPVVGRIIDRVGARKILLPSMAAFGVSMCSMYFLTGSLIHFYFMFAVV--- 127 + + PV+GR IDR G +K+L+PS V++ ++ FL L+ Y+ F + Sbjct: 60 LMMLFTAISLPVIGRFIDRFGVKKVLIPS-----VALQALCFLVAPLMQSYWQFIAIYIA 114 Query: 128 -GVVGAAANNVSYVRILSAWFVRHRGLSIGIASSGVAAGQSFGITLAQNVITDQGWRMAY 186 G + +N+V Y+R+LS WF R RGL+IGIA SG G ++ L + ++ GWR Y Sbjct: 115 IGTIAVGSNSVPYMRLLSTWFDRSRGLAIGIAGSGTGLGFAYVPILTEVLVNHFGWRGGY 174 Query: 187 VALGASVLLVGLPIVALFIKNDPK---DVGLEPCGADDVAGEKISLGYYVSKAEALRMPV 243 + LG +LLV LP+VA ++ P D + G D A +++S GY + EA+ Sbjct: 175 IGLGVILLLVTLPLVAFALRESPSHETDDSEQEAGTDG-AVKQVS-GYTLR--EAIFKRE 230 Query: 244 FWALIFLAFFMATALHGIQIHFVPLLLDAGLSPGQVTGLLVSVLSVGAIVGRVGTGFLFD 303 FW+L+ + +A L+G+ H VPLL D G+S + L + + R+ GFL D Sbjct: 231 FWSLVTIFMALAFVLYGLIPHLVPLLTDRGVSASKAAQLASAFGAAAFAG-RLMIGFLVD 289 Query: 304 RFFAPYVAIGVFGLPIIGLLLVLNHVGLWGLCAGAFFFGIGLGSESDVLGYFTSRYFGLK 363 R A +A F L +G+ + L + W L A G LG+E D+L Y TSRYFGL+ Sbjct: 290 RHDARVIAFIFFSLSALGIAIFLLPLPFWTLYCAAILLGGSLGAEVDMLAYLTSRYFGLR 349 Query: 364 SMAELYGYIFGAFMAGSSAGPALYGIVQGKTGNYDTTLFASIGMLMFVCLVCAFLPRFKD 423 S AE++ +F A M GP +G+V +G+Y + L I + +F ++ L + Sbjct: 350 SFAEVFSVLFSAVMVAMGLGPLAFGVVFDASGSYQSILMLGIPICVFAVIMLLTLRPYSQ 409 Query: 424 KNN 426 + Sbjct: 410 RRR 412 >ref|YP_001475257.1| major facilitator transporter [Shewanella sediminis HAW-EB3] gb|ABV38129.1| major facilitator superfamily MFS_1 [Shewanella sediminis HAW-EB3] Length = 486 Score = 221 bits (564), Expect = 1e-55, Method: Compositional matrix adjust. Identities = 139/410 (33%), Positives = 220/410 (53%), Gaps = 27/410 (6%) Query: 24 GWWMVAISFLGLAFGQTSLAFLTLGVFMKPFNQEFGWTRGQVAIALSVGAFVLLFTTPVV 83 GWW+V S +GL+ G + +A ++ G F+K F + FGWTRGQ++ +V + P Sbjct: 53 GWWIVLTSVIGLSLGYSVIA-MSFGTFIKEFEETFGWTRGQISFGSTVIGITAILFFPYA 111 Query: 84 GRIIDRVGARKILLPSMAAFGVSMCSMYFLTGSLIHFYFMFAVVGVVGAAANNVSYVRIL 143 G ++DR G + ILLPS FG+ M SM LT S+ HFY M + ++GA ++Y R+L Sbjct: 112 GMLVDRYGVKNILLPSTFLFGLVMASMSLLTPSIWHFYAMCIFIPLLGAGTAPLTYSRLL 171 Query: 144 SAWFVRHRGLSIGIASSGVAAGQSFGITLAQNVITDQGWRMAYVALGASVLLVGLPIVAL 203 +WF + RGL++GI SGV G + +A I WR AY+A+G ++L+ P+ Sbjct: 172 VSWFDKQRGLALGIGLSGVGLGTAIVPLIASYFIEHYSWRQAYLAIGGLIILLVWPMAKF 231 Query: 204 FIKNDPKDVGLEPCG---ADDVAGEK---------ISLGYYVSKAEALRMPVFWALIFLA 251 + + P + GL G +DD A K I +GY + EA++ FW ++ Sbjct: 232 VLADKPNEKGLVADGMALSDDEASAKLQARVEQIDIKVGY--TAKEAVKSKAFWLMLSSF 289 Query: 252 FFMATALHGIQIHFVPLLLDAGLSPGQVTGLLVSVLSVGAIVGRVGTGFLFDRFFAPYVA 311 F + +H VP+++D G S + + L + + GR+ G+L D+FFAPYV Sbjct: 290 FITGLTTSTLLVHLVPMMMDRGASLQEAVETF-AYLGISVVGGRLIAGYLMDKFFAPYV- 347 Query: 312 IGVFGL-PIIGLLLVLNHVGLWGLCAGAF--FFGIGLGSESDVLGYFTSRYFGLKSMAEL 368 + +F L PI+GL +G G A GI +G+E D + YFT++YFG +S + Sbjct: 348 VAIFMLGPILGLAAF--SMGATGDMAAISIGLIGIAIGAEFDAIAYFTTQYFGKRSFGVI 405 Query: 369 YGYIFGAFMAGSSAGPALYGIVQGKTGNYDTTLFA-----SIGMLMFVCL 413 YGY + F G+S GP + G+ + G Y L+A ++G ++ +C+ Sbjct: 406 YGYGYSGFKLGASIGPLIMGLTYDRLGRYTEVLWAMSATMALGCVLILCM 455