BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0065.p (302 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003543669.1| putative extradiol ring-cleavage dioxygenase... 222 4e-56 ref|YP_004833904.1| 2,2',3-trihydroxy-3'-methoxy-5,5'-dicarboxyb... 207 2e-51 ref|YP_004835035.1| 3-O-methylgallate 3,4-dioxygenase [Sphingobi... 196 5e-48 ref|YP_003608176.1| extradiol ring-cleavage dioxygenase class II... 191 1e-46 ref|YP_299962.1| protocatechuate 4,5-dioxygenase subunit beta [R... 190 2e-46 >ref|YP_003543669.1| putative extradiol ring-cleavage dioxygenase [Sphingobium japonicum UT26S] dbj|BAI95057.1| putative extradiol ring-cleavage dioxygenase [Sphingobium japonicum UT26S] Length = 316 Score = 222 bits (566), Expect = 4e-56, Method: Compositional matrix adjust. Identities = 109/296 (36%), Positives = 181/296 (61%), Gaps = 8/296 (2%) Query: 13 DFDGKRVQYDELLAKADPAIVKEIAPDVMARRHEANQKAMVELNRRLDAVSPDLVIVLGD 72 D +G+ Y LL +AD A+ I P ++ R A ++ + + + A D +IV+GD Sbjct: 23 DKEGRPATYAMLLERADKALRDHITPALLDERMRAAKRGVERIRAAIVASGIDTLIVIGD 82 Query: 73 DHKEVFNDDNMPALSIFCGDTLPYKPQ-----GIMKWKYDPRLNQDLWYPQEEKEYPIDS 127 D E++ +DN+P++ I+ G+T+ P+ ++W + R + + E ++P+D+ Sbjct: 83 DQAELYTNDNLPSILIYYGETIRNNPRHKGNPNPLEWWQNAR--GGYYVDEGETDFPVDA 140 Query: 128 KNALRLIGDLMNNGFDLAHSRYLAPEQGMSHSFGYVYYKIMTN-KIYPVIPISMNTYYPP 186 A +I L+++ FD++ + L +G H+FG+V+ ++M P++P+++NTYY P Sbjct: 141 GLARHMIARLVDDEFDISTANRLRDGEGEGHAFGFVHRQLMDEANPLPIVPLALNTYYAP 200 Query: 187 TQVTPRRSYQLGQALRKAVEAWPEDLKVAIISTGGLSHFVVDEKFDREFLDVMASGKIDG 246 Q TPRR Y LGQA+R+AVE++PED+KV +I++GGLSHFVVDE+ DR ++ + + + Sbjct: 201 NQPTPRRCYALGQAIRRAVESYPEDIKVGVIASGGLSHFVVDEELDRGLIEAIRAKDVAA 260 Query: 247 HLDIPLEKLQSGNSEFRCWSALAGAVEGMKMDLIDYVPCYRSPAGTGCGMAFATWQ 302 + KL SG+SE R W A AGA E + + +Y+PCYR+ AGTG G+ FA W Sbjct: 261 LTSMDRAKLNSGSSEIRNWIATAGACEHLDLAWSEYLPCYRTEAGTGTGVCFAEWH 316 >ref|YP_004833904.1| 2,2',3-trihydroxy-3'-methoxy-5,5'-dicarboxybiphenyl dioxygenase [Sphingobium sp. SYK-6] dbj|BAK65447.1| 2,2',3-trihydroxy-3'-methoxy-5,5'-dicarboxybiphenyl dioxygenase [Sphingobium sp. SYK-6] Length = 336 Score = 207 bits (526), Expect = 2e-51, Method: Compositional matrix adjust. Identities = 107/305 (35%), Positives = 177/305 (58%), Gaps = 17/305 (5%) Query: 12 HDFDGKRVQYDELLA-KADPAIVKEIAPDVMARRHEANQKAMVELNRRLDAVSPDLVIVL 70 H F G+ ++EL++ ++ + + + RR Q A+ ++ R A D+ +++ Sbjct: 35 HPFRGEEYSFEELVSLRSGEKLAEACSLPERERRAAGCQSAIADMADRFAAAKIDVAVIM 94 Query: 71 GDDHKEVFNDDNMPALSIFCGDTL---PYKPQGIMKWKYDPRLNQDLW--YPQEEKEYPI 125 G+D +E+F +D PA++++ G+T+ P P+ + P +++ W P E ++YP Sbjct: 95 GNDQRELFLEDVTPAITVYLGETIWDQPATPEQAAR--MPPGIHEAEWGHSPPERRDYPC 152 Query: 126 DSKNALRLIGDLMNNGFDLAHSRYLAPE------QGMSHSFGYVYYKIMTNKIYPVIPIS 179 + + + L+ GFDLA S+ L PE G HS G++Y +IM +++ P +PI Sbjct: 153 QPELGMHVCKTLVPQGFDLAVSKTL-PEPAGHWSSGAPHSLGFIYRQIMRDQVVPNLPII 211 Query: 180 MNTYYPPTQVTPRRSYQLGQALRKAVEAWPEDLKVAIISTGGLSHFVVDEKFDREFLDVM 239 +NT++PP Q T RR ++LG+A+ KA+ +W EDL+VA+ +GG+SHFV+DE FDR F + + Sbjct: 212 INTFFPPNQPTARRCFELGRAVGKAIRSWKEDLRVAVFGSGGMSHFVIDEDFDRMFFEAL 271 Query: 240 ASGKIDGHLDIPLEKLQSGNSEFRCWSALAGAV--EGMKMDLIDYVPCYRSPAGTGCGMA 297 + + I + LQSG SE + W A AGA+ +K D++ Y PCYRS AGTG Sbjct: 272 RNRDAETLCAIEDKHLQSGTSELKTWIAAAGALFDTDLKGDVVGYEPCYRSEAGTGTANG 331 Query: 298 FATWQ 302 F WQ Sbjct: 332 FVAWQ 336 >ref|YP_004835035.1| 3-O-methylgallate 3,4-dioxygenase [Sphingobium sp. SYK-6] dbj|BAC79261.1| 3-O-methylgallate 3,4-dioxygenase [Sphingomonas paucimobilis] dbj|BAK66578.1| 3-O-methylgallate 3,4-dioxygenase [Sphingobium sp. SYK-6] Length = 330 Score = 196 bits (497), Expect = 5e-48, Method: Compositional matrix adjust. Identities = 103/297 (34%), Positives = 164/297 (55%), Gaps = 7/297 (2%) Query: 12 HDFDGKRVQYDELLA-KADPAIVKEIAPDVMARRHEANQKAMVELNRRLDAVSPDLVIVL 70 H + G YD+L+A +AD +I P+ M + Q ++ +L D+ ++L Sbjct: 35 HAWRGGSWSYDQLVAERADQNFAAQITPEAMTAHNARCQASLDQLAEIFSEAKIDVAVIL 94 Query: 71 GDDHKEVFNDDNMPALSIFCGDTLP---YKPQGIMKWKYDPRLNQDLWYPQEEKEYPIDS 127 G+D E+F++ +PA S+F GDT+ + P+ + L+ + P EY Sbjct: 95 GNDQMEIFDERLVPAFSVFYGDTITNYEFPPERMAALPPGINLSVAGYIPSGGAEYAGQP 154 Query: 128 KNALRLIGDLMNNGFDLAHSRYLAPEQGMSHSFGYVYYKIMTNKIYPVIPISMNTYYPPT 187 + A +I M + FD+A + L P+ H+FG+VY +IM + P +P+ +NT+YPP Sbjct: 155 ELARSIIAQAMADEFDVAAMKAL-PKPETPHAFGFVYRRIMRDNPVPSVPVLVNTFYPPN 213 Query: 188 QVTPRRSYQLGQALRKAVEAWPEDLKVAIISTGGLSHFVVDEKFDREFLDVMASGKIDGH 247 Q T RR Y+ G+++ + ++AW D +VA++++GGL+HFV+DE+ DR F M I Sbjct: 214 QPTVRRCYEFGKSVLRGIQAWESDARVAVLASGGLTHFVIDEEIDRLFFQAMEDRDIARL 273 Query: 248 LDIPLEKLQSGNSEFRCWSALAG--AVEGMKMDLIDYVPCYRSPAGTGCGMAFATWQ 302 D+ Q G SE + W LAG A G+ +++DYVPCYRS AGTG M F W+ Sbjct: 274 ADLGEAIFQDGTSELKNWIPLAGMMAELGLDHEILDYVPCYRSEAGTGNAMGFVCWR 330 >ref|YP_003608176.1| extradiol ring-cleavage dioxygenase class III protein subunit B [Burkholderia sp. CCGE1002] gb|ADG18665.1| Extradiol ring-cleavage dioxygenase class III protein subunit B [Burkholderia sp. CCGE1002] Length = 334 Score = 191 bits (485), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 101/297 (34%), Positives = 169/297 (56%), Gaps = 8/297 (2%) Query: 12 HDFDGKRVQYDELLA-KADPAIVKEIAPDVMARRHEANQKAMVELNRRLDAVSPDLVIVL 70 H F+G+ +DEL+ + + ++I DV RH A+ E+ R L PD+V+++ Sbjct: 35 HHFEGREWSFDELVDYRRAENLGEQITLDVWKTRHAQCLAAIEEMARVLAEAKPDVVVIV 94 Query: 71 GDDHKEVFNDDNMPALSIFCGDTLPYKPQGIMKWKYDP---RLNQDLWYPQEEKEYPIDS 127 G+D E+F+ D +PALS+ CG T+ K + P R + P + Y Sbjct: 95 GNDQMEIFDTDCIPALSVMCGPTIVNNMISHEKLQTMPPGVRHAMPGYMP-DTATYSAVP 153 Query: 128 KNALRLIGDLMNNGFDLAHSRYLAPEQGMSHSFGYVYYKIMTNKIYPVIPISMNTYYPPT 187 + A +I MN+ FD+A + L+ E H+FG+V+ ++M +++ P +P+ +NT+YPP Sbjct: 154 ELAKHIIKTAMNDQFDVAALQCLS-ETETPHAFGFVFRQLMKDQVIPTVPVLVNTFYPPN 212 Query: 188 QVTPRRSYQLGQALRKAVEAWPEDLKVAIISTGGLSHFVVDEKFDREFLDVMASGKIDGH 247 Q T R + G+++ +A+++W D +VA+I++GGL+HFV+DE+ D+ L M + Sbjct: 213 QPTVARCHAFGRSIGRAIQSWDTDARVAVIASGGLTHFVIDERVDQLVLSAMKNHDFSPV 272 Query: 248 LDIPLEKLQSGNSEFRCWSALAGAVE--GMKMDLIDYVPCYRSPAGTGCGMAFATWQ 302 ++ Q+G SE + W +AG ++ G M ++DYVPCYRS AGTG M F W+ Sbjct: 273 FELDEAIFQAGTSEIKNWIPVAGVMDEIGFDMTVVDYVPCYRSEAGTGNAMGFVYWK 329 >ref|YP_299962.1| protocatechuate 4,5-dioxygenase subunit beta [Ralstonia eutropha JMP134] gb|AAZ65118.1| Extradiol ring-cleavage dioxygenase, class III enzyme, subunit B [Ralstonia eutropha JMP134] Length = 338 Score = 190 bits (483), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 105/303 (34%), Positives = 173/303 (57%), Gaps = 16/303 (5%) Query: 10 ALHDFDGKRVQYDELLA-KADPAIVKEIAPDVMARRHEANQKAMVELNRRLDAVSPDLVI 68 +LH + G+ +DEL+ +A + +I P+V RH +A+ ++R L PD+ + Sbjct: 33 SLHHYQGRTWTFDELVQHRASENLAAQITPEVWEARHTRCMQAIERMSRVLAEARPDVAV 92 Query: 69 VLGDDHKEVFNDDNMPALSIFCGDTLPYKPQGIMKW-KYDPRLNQDL-WYPQEEKEYPID 126 ++G+D E+F+D +PA S+ G+T+ K + P + + Y E Y Sbjct: 93 IVGNDQMEIFDDMLVPAFSVMWGETIVNNMISDEKLSRLPPGVAHAMPGYMPPEATYAGV 152 Query: 127 SKNALRLIGDLMNNGFDLAHSRYLAPEQGMSHSFGYVYYKIMTNKIYPVIPISMNTYYPP 186 LI + +GFD+ ++L+ + H+FG+V+ ++M +++ P +P+ +NT+YPP Sbjct: 153 PALGRHLIERTVADGFDITALKHLSATE-TPHAFGFVFRQLMKDQVIPTVPVLINTFYPP 211 Query: 187 TQVTPRRSYQLGQALRKAVEAWPEDLKVAIISTGGLSHFVVDEKFDREFLDVMASGKIDG 246 Q T R + G++L +A+ +W D +VAII++GGL+HFV+DE D+ LD M + Sbjct: 212 NQPTVGRCHAFGRSLARAIRSWDSDARVAIIASGGLTHFVIDETVDQLILDSMRA----- 266 Query: 247 HLDIPLEKL-----QSGNSEFRCWSALAGAVE--GMKMDLIDYVPCYRSPAGTGCGMAFA 299 H P+E L Q+G SE + W +AGA++ G M++IDYVPCYRS AGTG M FA Sbjct: 267 HDFAPVEGLGEAIFQAGTSEVKNWIPVAGAMDELGFGMEVIDYVPCYRSEAGTGNAMGFA 326 Query: 300 TWQ 302 W+ Sbjct: 327 CWR 329