BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0046.p (447 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_004359889.1| MFS superfamily proline/ betaine transporter... 554 e-155 ref|ZP_08879129.1| MFS superfamily proline/ betaine transporter ... 496 e-138 ref|ZP_08119296.1| MFS superfamily proline/ betaine transporter ... 362 5e-98 ref|ZP_08123981.1| general substrate transporter [Pseudonocardia... 338 9e-91 ref|ZP_07300725.1| proline-betaine transporter [Streptomyces hyg... 337 2e-90 >ref|YP_004359889.1| MFS superfamily proline/ betaine transporter [Burkholderia gladioli BSR3] gb|AEA59933.1| MFS superfamily proline/ betaine transporter [Burkholderia gladioli BSR3] Length = 442 Score = 554 bits (1428), Expect = e-155, Method: Compositional matrix adjust. Identities = 268/403 (66%), Positives = 330/403 (81%) Query: 44 IEYYEFGVYGYMAATIGPLFFPSTDATASLLAILAVFGSAFLMRPIGGIVLGRLGDRIGR 103 IEYYEFGVYGYMAA IGPLFFP+ + TA+LL+ILA+FGSAFLMRPIGGIVLGRLGDR+GR Sbjct: 39 IEYYEFGVYGYMAALIGPLFFPADNPTAALLSILAIFGSAFLMRPIGGIVLGRLGDRVGR 98 Query: 104 RGILLVTVIGMGIATGLIGLLPTASTAGILAPLMLLLVRLVQGFFAGGEVTGAAAYVAEC 163 R +LLVTV+GMGIAT +GLLPTA+TAG+ AP+ LLL RL QGFF+G EVTGAA Y+AE Sbjct: 99 RAVLLVTVLGMGIATAAVGLLPTAATAGVAAPVALLLARLAQGFFSGAEVTGAATYLAES 158 Query: 164 APPGKRGFYGAFTPVGVAVGGAVAAALCGITSVLLTPAQMAEWGWRIPFLLAIPMVLIST 223 AP G+RGFYGAFTPVGVA+GGA+AA +CG+T+ L+ QM GWRIPFL A+P+VL+S Sbjct: 159 APRGRRGFYGAFTPVGVAIGGALAATVCGLTTALVGTEQMHAGGWRIPFLAALPLVLLSA 218 Query: 224 AVRRRVEESAAFQEFQAHNEPVKAPLREVLTQHPGKIFKVMALAFGQNVGYYVGLVFMNI 283 +R+RV+ES AF++ A +E VKAPL E+ H + KV ++ GQN GY+VG +FMNI Sbjct: 219 LIRKRVDESVAFRQSAARHETVKAPLSELFAHHRAALLKVFLISLGQNAGYWVGFIFMNI 278 Query: 284 YMTTYLKYDKTAIYWIMAAVGVTMAMLMPFFGSLSDRWGRRPVLAIGFIGYMVLVWPMMF 343 Y+T+YLKYDK +++ +MA + + MA LMP +G LSDR GRR VL +GF GY+ L++PMM Sbjct: 279 YLTSYLKYDKASVFTLMAVISLAMAALMPLWGGLSDRIGRRRVLLVGFAGYIALIFPMMV 338 Query: 344 LMDQQSLPLAALAMFIMALPLPIVQSVGYPTYAEQFPTKVRYTGMAFSINIGAILGGGVT 403 LM+QQS LAALAMF++ALP+P VQSVGYPTYAEQFPT+VRYTGMAFS N GA+LGGGVT Sbjct: 339 LMNQQSFALAALAMFVLALPMPAVQSVGYPTYAEQFPTRVRYTGMAFSFNFGAMLGGGVT 398 Query: 404 PYLATSLMATTGNLKSPAFLLIAAAVLSLLALLTVHETAKEKL 446 PYLAT+L+ +TGNL SPAFL++ A V+ LALL + ETA L Sbjct: 399 PYLATALIGSTGNLMSPAFLMVGALVIGGLALLRMRETADAAL 441 >ref|ZP_08879129.1| MFS superfamily proline/ betaine transporter [Saccharopolyspora spinosa NRRL 18395] Length = 436 Score = 496 bits (1277), Expect = e-138, Method: Compositional matrix adjust. Identities = 268/420 (63%), Positives = 344/420 (81%) Query: 28 RATARKAAIAASAGTAIEYYEFGVYGYMAATIGPLFFPSTDATASLLAILAVFGSAFLMR 87 + T RKAAIAA GTAIEYYEFGVYGY+A TI PLFFPS + TA+LL+ LAVFGS+FL+R Sbjct: 16 QQTTRKAAIAAMTGTAIEYYEFGVYGYLAVTISPLFFPSNNPTAALLSTLAVFGSSFLVR 75 Query: 88 PIGGIVLGRLGDRIGRRGILLVTVIGMGIATGLIGLLPTASTAGILAPLMLLLVRLVQGF 147 P+GGI+LGRLGDRIGR+ +LL+TVIGMG AT + GLLPT ++AG+LAP LL+VRL+QGF Sbjct: 76 PLGGILLGRLGDRIGRKAVLLLTVIGMGSATAVTGLLPTHTSAGLLAPAALLIVRLLQGF 135 Query: 148 FAGGEVTGAAAYVAECAPPGKRGFYGAFTPVGVAVGGAVAAALCGITSVLLTPAQMAEWG 207 FAGGEVTGAAAYVAE AP G+RGF+G FTP+GVA+GGA AA + G+ S +LTP QM+ WG Sbjct: 136 FAGGEVTGAAAYVAESAPTGRRGFFGGFTPMGVAMGGAAAATVAGVVSSVLTPEQMSAWG 195 Query: 208 WRIPFLLAIPMVLISTAVRRRVEESAAFQEFQAHNEPVKAPLREVLTQHPGKIFKVMALA 267 WRIPFL +IP++++S +R+++E++ AF+ + + E VKAPLREVLT H + KV+ L+ Sbjct: 196 WRIPFLCSIPLIVLSLVLRKKIEDTHAFESAKENQELVKAPLREVLTGHLPAVAKVVGLS 255 Query: 268 FGQNVGYYVGLVFMNIYMTTYLKYDKTAIYWIMAAVGVTMAMLMPFFGSLSDRWGRRPVL 327 FGQN GY+VGLVFMNIY+T L Y++TA++WI+ AV ++M +LMP +G LSDR GR+ VL Sbjct: 256 FGQNAGYWVGLVFMNIYLTKNLGYNQTAVFWIIMAVNLSMGLLMPLYGGLSDRKGRKFVL 315 Query: 328 AIGFIGYMVLVWPMMFLMDQQSLPLAALAMFIMALPLPIVQSVGYPTYAEQFPTKVRYTG 387 IGF+GYMVLV PMM +M+ ++ LA LAM + A+P PIVQSVGYPTYAEQFPT+VRYTG Sbjct: 316 TIGFVGYMVLVTPMMLVMNHNNIWLAFLAMVVAAIPFPIVQSVGYPTYAEQFPTRVRYTG 375 Query: 388 MAFSINIGAILGGGVTPYLATSLMATTGNLKSPAFLLIAAAVLSLLALLTVHETAKEKLA 447 ++ S NIG ILGGG+ PY AT L++TT NL +P +LLI A ++LL+LL+V ETA+ +LA Sbjct: 376 LSLSFNIGTILGGGLMPYAATWLISTTDNLLAPGWLLIGACAIALLSLLSVRETARSELA 435 >ref|ZP_08119296.1| MFS superfamily proline/ betaine transporter [Pseudonocardia sp. P1] Length = 439 Score = 362 bits (930), Expect = 5e-98, Method: Compositional matrix adjust. Identities = 199/442 (45%), Positives = 293/442 (66%), Gaps = 7/442 (1%) Query: 7 LSSNSSPAAEAGIADKLDPQARATARKAAIAASAGTAIEYYEFGVYGYMAATIGPLFFPS 66 +S +SP A P + +AR+AA+A GT IEYY+F VY ++A T+GPLFFPS Sbjct: 1 MSETTSPPG----AGTSAPPSTLSARRAALAGGVGTLIEYYDFSVYAFLAVTLGPLFFPS 56 Query: 67 TDATASLLAILAVFGSAFLMRPIGGIVLGRLGDRIGRRGILLVTVIGMGIATGLIGLLPT 126 + S+L LAVFGSA++MRPIGG GRLGDR GR+ L+VTV+ MG+ +G++GLLP Sbjct: 57 DEPGVSILLTLAVFGSAYVMRPIGGWFFGRLGDRRGRKHALVVTVVTMGVFSGVLGLLPD 116 Query: 127 ASTAGILAPLMLLLVRLVQGFFAGGEVTGAAAYVAECAPPGKRGFYGAFTPVGVAVGGAV 186 +TAG+LAP++L+LVRL QGF AGGE+ GAA YVAE APPGKRG YG+ TPVG +G +V Sbjct: 117 FATAGVLAPVLLVLVRLAQGFSAGGEIGGAATYVAESAPPGKRGLYGSLTPVGSTLGFSV 176 Query: 187 AAALCGITSVLLTPAQMAEWGWRIPFLLAIPMVLISTAVRRRVEESAAFQEFQAHNEPVK 246 AAA+ G+ + TP QM+ WGWRIPFL+A+P+ ++ VR ++E++A F+ E V+ Sbjct: 177 AAAVVGLVTAFTTPEQMSAWGWRIPFLIAVPLAVVCLWVRVKLEDTAEFESMAERKEVVR 236 Query: 247 APLREVLTQHPGKIFKVMALAFGQNVGYYVGLVFMNIYMTTYLKYDKTAIYWIMAAVGVT 306 +PL V+ + PG + +V+ +A N YVGL + + Y+ +DK A+YW +A+G+ Sbjct: 237 SPLLSVVRERPGAVLRVVGIAIAMNGTGYVGLTYFSTYLIQTQGFDKQAVYW-ASAIGIA 295 Query: 307 MAM-LMPFFGSLSDRWGRRPVLAIGFIGYMVLVWPMMFLMDQQSLPLAALAMFIMALPLP 365 +A P G L+DR+GR+PVL + Y+V+ WP L+ S + ++++ + + Sbjct: 296 LACATYPLAGMLTDRFGRKPVLLSAYALYLVIAWPAFALLGATSSIVVVTLVYVVYMAVN 355 Query: 366 -IVQSVGYPTYAEQFPTKVRYTGMAFSINIGAILGGGVTPYLATSLMATTGNLKSPAFLL 424 + Q +P + E FP +VRYTG+A NIG IL GG PY++ L+ +TGN SPAF + Sbjct: 356 GLAQVPAFPQFTELFPRRVRYTGVALGFNIGTILAGGTAPYVSAQLVQSTGNPMSPAFWV 415 Query: 425 IAAAVLSLLALLTVHETAKEKL 446 + A++ ++ +LT+ ET +E+L Sbjct: 416 LGVALVGVVTVLTLRETGRERL 437 >ref|ZP_08123981.1| general substrate transporter [Pseudonocardia sp. P1] Length = 452 Score = 338 bits (868), Expect = 9e-91, Method: Compositional matrix adjust. Identities = 192/439 (43%), Positives = 281/439 (64%), Gaps = 2/439 (0%) Query: 10 NSSPAAEAG--IADKLDPQARATARKAAIAASAGTAIEYYEFGVYGYMAATIGPLFFPST 67 + P+ G ++ + A R+A+ AA+ GTAIEYY+F VYGY+A + PLFFPS Sbjct: 2 RNRPSEREGAPMSTQSSEDAVRVGRRASTAAAVGTAIEYYDFAVYGYLAVVLAPLFFPSQ 61 Query: 68 DATASLLAILAVFGSAFLMRPIGGIVLGRLGDRIGRRGILLVTVIGMGIATGLIGLLPTA 127 + +L LAV S FL RP+GG+ GRLGDR GRR +L+ TV MG+AT L GLLPT Sbjct: 62 EGLIGILGTLAVVASGFLARPLGGLFFGRLGDRRGRRAVLMATVTVMGVATVLTGLLPTY 121 Query: 128 STAGILAPLMLLLVRLVQGFFAGGEVTGAAAYVAECAPPGKRGFYGAFTPVGVAVGGAVA 187 +T G+LAP++L ++R+VQGF AGGE+ GAA+ E AP +RG +G+ T +G++VG A A Sbjct: 122 ATIGVLAPILLTVLRIVQGFSAGGEIGGAASLSTESAPSRRRGLFGSATSIGISVGLAGA 181 Query: 188 AALCGITSVLLTPAQMAEWGWRIPFLLAIPMVLISTAVRRRVEESAAFQEFQAHNEPVKA 247 A G + LL+P QM+ WGWR+PFL+A P+++ + R RVE+S FQ+ EP KA Sbjct: 182 AGTVGALTSLLSPDQMSSWGWRVPFLIAGPLLVGALLYRMRVEDSPMFQQMVEQAEPPKA 241 Query: 248 PLREVLTQHPGKIFKVMALAFGQNVGYYVGLVFMNIYMTTYLKYDKTAIYWIMAAVGVTM 307 P+ EVL +H +V+ +A+ + V++ ++++ L Y + W++ + + Sbjct: 242 PVSEVLREHKAATLRVVGIAYATMTTGGLASVYLLVHLSAVLGYPLSGSLWLIVLIVLMP 301 Query: 308 AMLMPFFGSLSDRWGRRPVLAIGFIGYMVLVWPMMFLMDQQSLPLAALAMFIMALPLPIV 367 L+P+ G+LSDR+GRR VLA G +G++VL P ++M Q SL LA +A ++ +P I Sbjct: 302 LGLIPWSGALSDRFGRRRVLAAGLVGFLVLGVPCFWVMQQGSLLLAIVAALVLNIPFGIQ 361 Query: 368 QSVGYPTYAEQFPTKVRYTGMAFSINIGAILGGGVTPYLATSLMATTGNLKSPAFLLIAA 427 Q V Y Y+E FPT+VRYTG++ NIG ++G G +ATSL+A TGN +PA L+ A Sbjct: 362 QGVVYTQYSELFPTRVRYTGVSLGFNIGGVIGSGAVSVVATSLVAATGNTLAPAAYLVLA 421 Query: 428 AVLSLLALLTVHETAKEKL 446 AV+ LL + T+ ET+ L Sbjct: 422 AVVGLLVVATMRETSTTDL 440 >ref|ZP_07300725.1| proline-betaine transporter [Streptomyces hygroscopicus ATCC 53653] gb|EFL29094.1| proline-betaine transporter [Streptomyces himastatinicus ATCC 53653] Length = 433 Score = 337 bits (865), Expect = 2e-90, Method: Compositional matrix adjust. Identities = 191/434 (44%), Positives = 263/434 (60%), Gaps = 3/434 (0%) Query: 7 LSSNSSPAAEAGIADKLDPQARATARKAAIAASAGTAIEYYEFGVYGYMAATIGPLFFPS 66 +S+ PA + DP+ T R+A +A S GT IEYY+F VYGY+A I P FFP+ Sbjct: 1 MSTTEQPAPPGSASTSYDPR---TVRRAMLAGSIGTLIEYYDFSVYGYLAVVIAPQFFPN 57 Query: 67 TDATASLLAILAVFGSAFLMRPIGGIVLGRLGDRIGRRGILLVTVIGMGIATGLIGLLPT 126 D TAS LA LAV + ++RP+GG+ G LGDR GRR L+ +V+ MG+A+ + GLLPT Sbjct: 58 EDPTASTLAALAVLATGLVVRPLGGVFFGWLGDRWGRRKALVSSVLCMGVASSITGLLPT 117 Query: 127 ASTAGILAPLMLLLVRLVQGFFAGGEVTGAAAYVAECAPPGKRGFYGAFTPVGVAVGGAV 186 G+LA ++L L RLVQG +GGE GA Y+ E APP +R F GA TP+G +G Sbjct: 118 YGQIGVLAAVLLFLTRLVQGISSGGESVGAYTYIYESAPPRRRAFLGAVTPIGSNLGFMF 177 Query: 187 AAALCGITSVLLTPAQMAEWGWRIPFLLAIPMVLISTAVRRRVEESAAFQEFQAHNEPVK 246 AAA G S L T QMA+WGWR+PFL+A+P+ LI R R+E++ F+E + Sbjct: 178 AAATAGAISALTTQDQMADWGWRLPFLMAVPLTLICLWARLRIEDTPEFKEAAKRADIPA 237 Query: 247 APLREVLTQHPGKIFKVMALAFGQNVGYYVGLVFMNIYMTTYLKYDKTAIYWIMAAVGVT 306 AP+REVL+ H + + + LA Q ++GL +M IY+TT L YD T ++W+ A V + Sbjct: 238 APIREVLSTHRTALLQSIGLAMAQGGAIFLGLTYMGIYLTTNLGYDPTHVFWLTAGVVLV 297 Query: 307 MAMLMPFFGSLSDRWGRRPVLAIGFIGYMVLVWPMMFLMDQQSLPLAALAMFIMALPLPI 366 +LM G L+ R+G R VL G +G++V +P + LM + SL +A LA + L Sbjct: 298 TVLLMVPAGWLAARFGCRRVLLAGLLGFLVTAYPALTLMGRHSLAVAGLAYLLFMLSSAF 357 Query: 367 VQSVGYPTYAEQFPTKVRYTGMAFSINIGAILGGGVTPYLATSLMATTGNLKSPAFLLIA 426 VQ + F +VRYTGMA NIG +L GG PY+A L+ TGNL SPAF ++A Sbjct: 358 VQVPAASLWPHLFERRVRYTGMAIGYNIGTVLAGGTAPYIAAFLVQRTGNLLSPAFFVMA 417 Query: 427 AAVLSLLALLTVHE 440 ++ L AL TV + Sbjct: 418 VCLIGLGALSTVRK 431