BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0021.p (368 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_001352714.1| component of multidrug efflux system [Janthi... 382 e-104 ref|YP_004201125.1| RND family efflux transporter MFP subunit [G... 333 3e-89 ref|YP_001766740.1| RND family efflux transporter MFP subunit [M... 322 8e-86 ref|YP_003070209.1| multidrug efflux system protein [Methylobact... 311 8e-83 ref|YP_001641244.1| RND family efflux transporter MFP subunit [M... 310 2e-82 >ref|YP_001352714.1| component of multidrug efflux system [Janthinobacterium sp. Marseille] gb|ABR91562.1| component of multidrug efflux system [Janthinobacterium sp. Marseille] Length = 367 Score = 382 bits (981), Expect = e-104, Method: Compositional matrix adjust. Identities = 197/344 (57%), Positives = 253/344 (73%), Gaps = 1/344 (0%) Query: 16 LIATLAACSKPAPPPATAPRVASFTVQNVEHDSASYTGVVHARTESNLGFRVPGKIVERL 75 L +L C KP +VA F + ASYTGVVHARTESNLGFR+ GKI++R Sbjct: 17 LSLSLPGCGKPVEKTEPLAKVAYFNAIAATSNGASYTGVVHARTESNLGFRISGKILQRF 76 Query: 76 VDPGELVKRGQALFKLDSTDYTLAQHAARSAVIAAQARDVQAQADEVRLRKLLNSGAVSR 135 VDPG+ VK GQ L K+D DY LA++A+R+ V AA+ARD+QA +DE+RLRKLL +GAVS+ Sbjct: 77 VDPGDSVKVGQPLMKIDPVDYELAKNASRALVQAAKARDLQASSDELRLRKLLETGAVSK 136 Query: 136 QAYEQSKALADSAKAQLDAERARAGQVENQSDYSVLRADADGVVMDVLADAGQVVGAGQT 195 QAYEQ KA ADSA AQL+A ++++ Q ENQ+DY++LRAD GVVMDV + GQ+V AGQT Sbjct: 137 QAYEQVKAAADSAAAQLEAAQSQSKQTENQADYTILRADVKGVVMDVPVEPGQIVSAGQT 196 Query: 196 IVRLAQKGPREAVVSIPENGLGQARRQASATLYTDDTQKFAAKLRELSAMADPLTRTYQA 255 +V+LA+ GPREAVVSIPE L R ASA+LY+D FAAKLRELS++ADPLTRTYQA Sbjct: 197 VVKLAKSGPREAVVSIPEQALKLLPRAASASLYSDSKTNFAAKLRELSSVADPLTRTYQA 256 Query: 256 RYVLSGKGEQAPLGSTVTIRLTGDTTSTEQ-TEVPIGALIDKGHGASVWLIEKQTQVVKL 314 +Y L+G+G+QAPLG+TVTI TGD+ S+ P+ AL+DKG G SVW+I+K V L Sbjct: 257 KYALAGEGDQAPLGATVTIHTTGDSNSSNMLIASPVSALVDKGDGPSVWVIDKANSTVSL 316 Query: 315 VPVTVVRLGEEFAAISAGLKAGDVVVAYGTHLLKAGDKVSVVSP 358 V V LGEE I++G+ G+ +VA+G+HLLK G KV +++P Sbjct: 317 RKVKVASLGEEVVGIASGIATGERIVAFGSHLLKQGQKVELLAP 360 >ref|YP_004201125.1| RND family efflux transporter MFP subunit [Geobacter sp. M18] gb|ADW15849.1| efflux transporter, RND family, MFP subunit [Geobacter sp. M18] Length = 384 Score = 333 bits (853), Expect = 3e-89, Method: Compositional matrix adjust. Identities = 184/365 (50%), Positives = 250/365 (68%), Gaps = 10/365 (2%) Query: 3 INKKYMGMALS---GALIATL--AACSKPAPPPA--TAPR-VASFTVQNVEHDSASYTGV 54 ++++Y + L+ GAL+ + A K PPA APR V +FTV + + YTGV Sbjct: 12 VSRRYRILLLTAVIGALVVVVCGAVFGKEKNPPAETAAPRLVRAFTVVSAQSGVEEYTGV 71 Query: 55 VHARTESNLGFRVPGKIVERLVDPGELVKRGQALFKLDSTDYTLAQHAARSAVIAAQARD 114 +HARTES+LGFRV GKI+ +LV G+ VK GQAL +LD TD L ++A++ V AA+A++ Sbjct: 72 IHARTESDLGFRVSGKILAKLVKAGDHVKSGQALMQLDPTDLQLTANSAQATVEAARAQN 131 Query: 115 VQAQADEVRLRKLLNSGAVSRQAYEQSKALADSAKAQLDAERARAGQVENQSDYSVLRAD 174 +A ADE+R R+L+ AVS Q YEQ+K+ AD+ AQL++ A A Q ENQ+ Y+VLRAD Sbjct: 132 KRALADELRQRELVAIKAVSVQEYEQAKSAADATTAQLNSAIAYARQSENQARYAVLRAD 191 Query: 175 ADGVVMDVLADAGQVVGAGQTIVRLAQKGPREAVVSIPENGLGQARRQASATLYTDDTQK 234 ADGV+MD+ AD GQVVG G +VRLA GPREAVV++PE A+ A+A+LY D Q Sbjct: 192 ADGVIMDIPADVGQVVGPGNVVVRLAHDGPREAVVNLPEGKEKMAKSTATASLYADSGQT 251 Query: 235 FAAKLRELSAMADPLTRTYQARYVLSGKGEQAPLGSTVTIRLTGDTT--STEQTEVPIGA 292 F A+LRELSAMAD LTRTYQARY L G G APLG+TVT+ L G++ + +Q E+P+GA Sbjct: 252 FPARLRELSAMADSLTRTYQARYSLGGVGTGAPLGATVTVHLDGESAHGTVQQYEIPVGA 311 Query: 293 LIDKGHGASVWLIEKQTQVVKLVPVTVVRLGEEFAAISAGLKAGDVVVAYGTHLLKAGDK 352 L D G G SVW++ T + PV V +LG E A +S G+K G+ ++A G HL+K G++ Sbjct: 312 LYDGGAGTSVWVLNPDTSTLSRRPVAVAKLGSETALVSKGIKPGERILALGAHLVKEGER 371 Query: 353 VSVVS 357 V ++S Sbjct: 372 VKILS 376 >ref|YP_001766740.1| RND family efflux transporter MFP subunit [Methylobacterium radiotolerans JCM 2831] gb|ACB27938.1| efflux transporter, RND family, MFP subunit [Methylobacterium radiotolerans JCM 2831] Length = 363 Score = 322 bits (824), Expect = 8e-86, Method: Compositional matrix adjust. Identities = 192/359 (53%), Positives = 244/359 (67%), Gaps = 15/359 (4%) Query: 10 MALSGALIA-----TLAACSKPA-----PPPATAPRVASFTVQNVEHDSASYTGVVHART 59 MA+ G + LAACS PA P P P V + V +A YTGV+ ART Sbjct: 1 MAMDGRWVIGFAGLALAACS-PAETAVTPEP---PLVQTVAVAPATAAAARYTGVIRART 56 Query: 60 ESNLGFRVPGKIVERLVDPGELVKRGQALFKLDSTDYTLAQHAARSAVIAAQARDVQAQA 119 ESNLGFRV GKI ERLVDPG+ V+ GQ L +LD TD+TLA +AAR++V AA+A+ ++A+A Sbjct: 57 ESNLGFRVGGKIFERLVDPGDHVRLGQPLMRLDRTDFTLALNAARASVEAARAQMIKARA 116 Query: 120 DEVRLRKLLNSGAVSRQAYEQSKALADSAKAQLDAERARAGQVENQSDYSVLRADADGVV 179 DE R RKL+ G S+Q Y+Q++A AD+A AQ A+A Q+ NQ+ YS L+ADADGVV Sbjct: 117 DEERSRKLVGDGWTSKQTYDQNRAAADAAIAQFANAEAQASQIANQAGYSELQADADGVV 176 Query: 180 MDVLADAGQVVGAGQTIVRLAQKGPREAVVSIPENGLGQARRQASATLYTDDTQKFAAKL 239 M+V ++ GQVV AGQT+V+LA+ G REA V +PE +AR ASATLY + + AKL Sbjct: 177 MEVPSEPGQVVAAGQTVVKLARDGAREAEVFVPEGSRQRARGTASATLYAEGESTYPAKL 236 Query: 240 RELSAMADPLTRTYQARYVLSGKGEQAPLGSTVTIRLT-GDTTSTEQTEVPIGALIDKGH 298 RELSA ADP TRTY+ARY+LSG GE APLG+TVT++LT + VP+GAL D+G Sbjct: 237 RELSATADPATRTYRARYILSGGGETAPLGATVTLQLTPAQAQARTWVSVPLGALFDQGG 296 Query: 299 GASVWLIEKQTQVVKLVPVTVVRLGEEFAAISAGLKAGDVVVAYGTHLLKAGDKVSVVS 357 G++VW + +TQ V PVTV RL EE A I++GL GD +VA G HLLKAG KV S Sbjct: 297 GSAVWRYDAETQTVAAQPVTVARLSEESAEIASGLNPGDRIVALGAHLLKAGQKVRPAS 355 >ref|YP_003070209.1| multidrug efflux system protein [Methylobacterium extorquens DM4] emb|CAX26390.1| component of multidrug efflux system [Methylobacterium extorquens DM4] Length = 350 Score = 311 bits (798), Expect = 8e-83, Method: Compositional matrix adjust. Identities = 181/340 (53%), Positives = 233/340 (68%), Gaps = 10/340 (2%) Query: 20 LAACSKPAPPPATAPRVASFTVQNVEHDSAS-----YTGVVHARTESNLGFRVPGKIVER 74 LAACS P TA VQ E +S YTGV+ ARTESNLGFRV GKI ER Sbjct: 3 LAACS----PAETAVNPEPPLVQVTEVAPSSGVVSRYTGVIRARTESNLGFRVGGKIFER 58 Query: 75 LVDPGELVKRGQALFKLDSTDYTLAQHAARSAVIAAQARDVQAQADEVRLRKLLNSGAVS 134 LVDPG+ V GQ L +LD TD+TLA +AAR++V AA+A+ ++A+ADE R RKL+ G S Sbjct: 59 LVDPGDRVHLGQPLMRLDRTDFTLALNAARASVEAARAQMIKAKADEERSRKLVADGWTS 118 Query: 135 RQAYEQSKALADSAKAQLDAERARAGQVENQSDYSVLRADADGVVMDVLADAGQVVGAGQ 194 +Q Y+Q+K AD+A AQ A+AGQ+ENQ+ Y+ L+ADADGVVM+V + G+VV AGQ Sbjct: 119 KQTYDQNKGAADAAIAQFANAEAQAGQIENQAGYAELKADADGVVMEVPSYPGEVVAAGQ 178 Query: 195 TIVRLAQKGPREAVVSIPENGLGQARRQASATLYTDDTQKFAAKLRELSAMADPLTRTYQ 254 T+VRLA+ G REA + +PE G A+ +A ATLY + + A+LRELSA+ADP TRTY+ Sbjct: 179 TVVRLARDGSREAEIFLPEASRGLAKGEAKATLYAEGEATYPARLRELSAIADPATRTYR 238 Query: 255 ARYVLSGKGEQAPLGSTVTIRL-TGDTTSTEQTEVPIGALIDKGHGASVWLIEKQTQVVK 313 ARY+LSG GE A LG+TVT++L + D T EVP+ AL D+G G SVW + ++ VK Sbjct: 239 ARYILSGGGEDASLGATVTLQLKSPDAVRTATVEVPLAALFDQGRGTSVWRYDAASETVK 298 Query: 314 LVPVTVVRLGEEFAAISAGLKAGDVVVAYGTHLLKAGDKV 353 V V R+GEE A ++AGL GD +VA G HLLK+ KV Sbjct: 299 PQAVQVARMGEERAEVAAGLNPGDRIVALGAHLLKSDQKV 338 >ref|YP_001641244.1| RND family efflux transporter MFP subunit [Methylobacterium extorquens PA1] gb|ABY32173.1| efflux transporter, RND family, MFP subunit [Methylobacterium extorquens PA1] Length = 361 Score = 310 bits (795), Expect = 2e-82, Method: Compositional matrix adjust. Identities = 179/341 (52%), Positives = 231/341 (67%), Gaps = 12/341 (3%) Query: 20 LAACS------KPAPPPATAPRVASFTVQNVEHDSASYTGVVHARTESNLGFRVPGKIVE 73 LAACS P PP VA + + YTGV+ ARTESNLGFRV GKI E Sbjct: 14 LAACSLAETAVNPEPPLVQVTEVAPSS-----GVVSRYTGVIRARTESNLGFRVGGKIFE 68 Query: 74 RLVDPGELVKRGQALFKLDSTDYTLAQHAARSAVIAAQARDVQAQADEVRLRKLLNSGAV 133 RLVDPG+ V GQ L +LD TD+TLA +AAR++V AA+A+ ++A+ADE R RKL+ G Sbjct: 69 RLVDPGDRVHLGQPLMRLDRTDFTLALNAARASVEAARAQMIKAKADEERSRKLVADGWT 128 Query: 134 SRQAYEQSKALADSAKAQLDAERARAGQVENQSDYSVLRADADGVVMDVLADAGQVVGAG 193 S+Q Y+Q+K AD+A AQ A+AGQ+ENQ+ Y+ L+ADADGVVM+V + G+VV AG Sbjct: 129 SKQTYDQNKGAADAAIAQFANAEAQAGQIENQAGYAELKADADGVVMEVPSYPGEVVAAG 188 Query: 194 QTIVRLAQKGPREAVVSIPENGLGQARRQASATLYTDDTQKFAAKLRELSAMADPLTRTY 253 QT+VRLA+ G REA + +PE G A+ +A ATLY + + A+LRELSA+ADP TRTY Sbjct: 189 QTVVRLARDGSREAEIFLPEASRGLAKGEAKATLYAEGEATYPARLRELSAIADPATRTY 248 Query: 254 QARYVLSGKGEQAPLGSTVTIRL-TGDTTSTEQTEVPIGALIDKGHGASVWLIEKQTQVV 312 +ARY+LSG GE A LG+TVT++L + D T EVP+ AL D+G G SVW + ++ V Sbjct: 249 RARYILSGGGEDASLGATVTLQLKSPDAVRTATVEVPLAALFDQGRGTSVWRYDAASETV 308 Query: 313 KLVPVTVVRLGEEFAAISAGLKAGDVVVAYGTHLLKAGDKV 353 K V V R+ EE A ++AGL GD +VA G HLLKA KV Sbjct: 309 KPQAVEVARMSEERAEVAAGLNPGDRIVALGAHLLKADQKV 349