BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0008.p (290 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_001629828.1| putative acyl dehydratase [Bordetella petrii... 345 4e-93 ref|ZP_06845968.1| 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-ch... 338 5e-91 ref|YP_002551624.1| 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-c... 338 7e-91 ref|YP_552039.1| MaoC-like dehydratase [Polaromonas sp. JS666] >... 338 7e-91 ref|YP_984452.1| dehydratase [Acidovorax sp. JS42] >gi|120604636... 338 8e-91 >ref|YP_001629828.1| putative acyl dehydratase [Bordetella petrii DSM 12804] emb|CAP41558.1| putative acyl dehydratase [Bordetella petrii] Length = 286 Score = 345 bits (886), Expect = 4e-93, Method: Compositional matrix adjust. Identities = 163/290 (56%), Positives = 209/290 (72%), Gaps = 4/290 (1%) Query: 1 MTIDYHHLKQRQFPEVRQSYTEKDCMLYALGLGLGADPTDRGQLQFVYEEGLKAMPSMAA 60 M ++Y +K FP+V Q+Y+EKD MLYAL +G G DP D+ QL FVYE+GLK PSM Sbjct: 1 MPLNYDLIKNWLFPDVEQAYSEKDSMLYALSVGYGHDPVDQDQLAFVYEKGLKTAPSMPV 60 Query: 61 ILGYPGFWIKDPDTGADWKQLLHGEQRLRLHATLPPSGTVIGRMSVKSITDKGAGKGAII 120 ILGYPGFW+KDP G DW +LLH Q L+LHA +PP+G V+GR VK+I DKGA KGA++ Sbjct: 61 ILGYPGFWMKDPAAGIDWVKLLHVGQALQLHAPIPPAGMVVGRTRVKAIIDKGAEKGALV 120 Query: 121 VTERRLTDAVSGALLAEIEMLTFCRGDGGYSAGGQPSDSLDSALPSPPDTAPDAVCDLPT 180 V ER++ + +SG LL+ +E T CRGDGG+ AG DS P PD APD+VCDLPT Sbjct: 121 VQERQILEKISGTLLSTLEQTTLCRGDGGFGAG----DSAPKPPPKMPDRAPDSVCDLPT 176 Query: 181 RPESALIYRLSGDANPLHADPDVAATAGFERPILHGLATYGIAAHAILKTWCGYQPEQLT 240 P+SAL YRL D NP+HADP +A +AG+ RPILHGL ++G+AAHA+L C Y P +L Sbjct: 177 LPQSALFYRLCADPNPVHADPQLARSAGYARPILHGLCSFGVAAHAVLGRCCAYDPLRLA 236 Query: 241 ALDVRFSAPVYPGETIRTEMWRQGDRIAFRARVLERDRIVLNNGMARLRT 290 +L VRFS PVYPGET+RTE+WR + +AFR +ER+ +VL++G A L Sbjct: 237 SLSVRFSNPVYPGETLRTEIWRSDESVAFRTWSVERNVVVLDHGNATLNV 286 >ref|ZP_06845968.1| 3-alpha,7-alpha, 12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoAhydratase [Burkholderia sp. Ch1-1] gb|EFG66400.1| 3-alpha,7-alpha, 12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoAhydratase [Burkholderia sp. Ch1-1] Length = 293 Score = 338 bits (868), Expect = 5e-91, Method: Compositional matrix adjust. Identities = 161/288 (55%), Positives = 204/288 (70%) Query: 1 MTIDYHHLKQRQFPEVRQSYTEKDCMLYALGLGLGADPTDRGQLQFVYEEGLKAMPSMAA 60 M ++Y +K F E+ +YT KD MLYAL LG+G+DPTD GQL FVYE+ L A+P+MA Sbjct: 1 MPLNYQAVKNWDFGEIEHTYTHKDTMLYALALGVGSDPTDPGQLPFVYEKNLVALPTMAV 60 Query: 61 ILGYPGFWIKDPDTGADWKQLLHGEQRLRLHATLPPSGTVIGRMSVKSITDKGAGKGAII 120 ILG+PGFW+ DP TG Q++HGEQ LRLH L P+GT+I R +V+S+ DKG +GA++ Sbjct: 61 ILGFPGFWMSDPRTGITASQIVHGEQSLRLHRALSPAGTIIARSAVRSVIDKGRDRGALV 120 Query: 121 VTERRLTDAVSGALLAEIEMLTFCRGDGGYSAGGQPSDSLDSALPSPPDTAPDAVCDLPT 180 V ER++TD SG LLA +E TFCR DGGYS GQPSD +A + P A D + + T Sbjct: 121 VIERKVTDKFSGHLLATLEQTTFCRADGGYSESGQPSDPPLAAPQAVPARAADVIVETTT 180 Query: 181 RPESALIYRLSGDANPLHADPDVAATAGFERPILHGLATYGIAAHAILKTWCGYQPEQLT 240 RPE AL+YRL GD NPLH DP+ A AGFERPILHGLATYG+AA AI++ + G P +L Sbjct: 181 RPEMALLYRLCGDVNPLHVDPETARRAGFERPILHGLATYGVAARAIVERFGGGVPSRLR 240 Query: 241 ALDVRFSAPVYPGETIRTEMWRQGDRIAFRARVLERDRIVLNNGMARL 288 +L RFSAPVYPGET+RTE+W I FR ER+ + +NNG+A L Sbjct: 241 SLRARFSAPVYPGETLRTELWASSGAIQFRVTATERESVAINNGVAEL 288 >ref|YP_002551624.1| 3-alpha,7-alpha, 12-alpha-trihydroxy-5-beta-cholest-24-enoyl-coahydratase [Acidovorax ebreus TPSY] gb|ACM31624.1| 3-alpha,7-alpha, 12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoAhydratase [Acidovorax ebreus TPSY] Length = 297 Score = 338 bits (866), Expect = 7e-91, Method: Compositional matrix adjust. Identities = 181/297 (60%), Positives = 220/297 (74%), Gaps = 10/297 (3%) Query: 1 MTIDYHHLKQRQFPEVRQSYTEKDCMLYALGLGLGADPTDRGQLQFVYEE---GLKAMPS 57 M IDYHHLK R F +RQ+YTE+D MLYAL LGLG DP + L FVYE GL+A+PS Sbjct: 1 MAIDYHHLKARDFGVIRQTYTERDTMLYALSLGLGNDPLNASALPFVYEGLEGGLRALPS 60 Query: 58 MAAILGYPGFWIKDPDTGADWKQLLHGEQRLRLHATLPPSGTVIGRMSVKSITDKGAGKG 117 A +LGYPGFW ++PDTG DW +LLHGEQR+RLH LP SG V+G + +TDKG GKG Sbjct: 61 QAVVLGYPGFWAREPDTGIDWVKLLHGEQRMRLHRPLPASGEVVGHNRITHLTDKGEGKG 120 Query: 118 AIIVTERRLTDAVSGALLAEIEMLTFCRGDGGYSAGGQ--PSDSLDSAL-PSPPDTAPDA 174 AI+VTERRL A +G LLA ++ +TF RGDGGYS G PSD+ AL P+P D APD Sbjct: 121 AIMVTERRLETA-AGELLATVQQVTFLRGDGGYSQQGGGQPSDAPLPALQPTPQDRAPDF 179 Query: 175 VCDLPTRPESALIYRLSGDANPLHADPDVAATAGFERPILHGLATYGIAAHAILKTWCGY 234 PTRPE+AL+YRL GD NPLHADP VAA AGFERPILHGLA+YG+ AHA+L+ C Sbjct: 180 TDTQPTRPEAALLYRLMGDYNPLHADPAVAAKAGFERPILHGLASYGLVAHAVLRQCCAG 239 Query: 235 QPEQLTALDVRFSAPVYPGETIRTEMWR---QGDRIAFRARVLERDRIVLNNGMARL 288 P +L A+D+RF+APVYPGET+ TE+WR Q ++ RARVLERD++VL++G A L Sbjct: 240 DPARLKAMDIRFAAPVYPGETLVTEIWRVPGQPAQVQLRARVLERDKVVLSHGFAEL 296 >ref|YP_552039.1| MaoC-like dehydratase [Polaromonas sp. JS666] gb|ABE47141.1| MaoC-like dehydratase [Polaromonas sp. JS666] Length = 285 Score = 338 bits (866), Expect = 7e-91, Method: Compositional matrix adjust. Identities = 165/289 (57%), Positives = 204/289 (70%), Gaps = 4/289 (1%) Query: 1 MTIDYHHLKQRQFPEVRQSYTEKDCMLYALGLGLGADPTDRGQLQFVYEEGLKAMPSMAA 60 M I+Y L F +VR YT++D MLYALG+GLGADPTD +L+FVYE+ L A+P++ Sbjct: 1 MAINYDQLMAWPFEDVRHRYTQRDTMLYALGVGLGADPTDETELRFVYEKNLLALPTLPV 60 Query: 61 ILGYPGFWIKDPDTGADWKQLLHGEQRLRLHATLPPSGTVIGRMSVKSITDKGAGKGAII 120 +LGYPG W+KDP TG DW +L+HGEQ LRLH +PP G VIGR V I DKG GKGA+I Sbjct: 61 VLGYPGMWLKDPATGVDWVRLVHGEQGLRLHRPVPPEGEVIGRTRVSQIIDKGPGKGALI 120 Query: 121 VTERRLTDAVSGALLAEIEMLTFCRGDGGYSAGGQPSDSLDSALPSPPDTAPDAVCDLPT 180 TER + DA SG LLA + TFCR DGG+ P ++ P A D D T Sbjct: 121 YTERTVLDAASGELLATLTSTTFCRADGGFGGPAGPVKTVHEL----PARAADHSVDFAT 176 Query: 181 RPESALIYRLSGDANPLHADPDVAATAGFERPILHGLATYGIAAHAILKTWCGYQPEQLT 240 +P +ALIYRLSGD NPLHA+P VA+ AGF++PILHGLATYGIA A++K CG P + Sbjct: 177 QPRAALIYRLSGDYNPLHAEPAVASAAGFKQPILHGLATYGIAGWAVVKQVCGGDPATVQ 236 Query: 241 ALDVRFSAPVYPGETIRTEMWRQGDRIAFRARVLERDRIVLNNGMARLR 289 ++DVRFS+PVYPGETIRTE+W G ++FRAR +ERD +VLNNG A LR Sbjct: 237 SIDVRFSSPVYPGETIRTELWVDGKVLSFRARAVERDIVVLNNGHAELR 285 >ref|YP_984452.1| dehydratase [Acidovorax sp. JS42] gb|ABM40376.1| MaoC domain protein dehydratase [Acidovorax sp. JS42] Length = 297 Score = 338 bits (866), Expect = 8e-91, Method: Compositional matrix adjust. Identities = 183/297 (61%), Positives = 219/297 (73%), Gaps = 10/297 (3%) Query: 1 MTIDYHHLKQRQFPEVRQSYTEKDCMLYALGLGLGADPTDRGQLQFVYEE---GLKAMPS 57 M IDY HLK R F VRQ YTE+D MLYAL LGLG DP + L FVYE GL+A+PS Sbjct: 1 MAIDYQHLKSRVFTPVRQHYTERDTMLYALSLGLGNDPLNASALPFVYEGLEGGLRALPS 60 Query: 58 MAAILGYPGFWIKDPDTGADWKQLLHGEQRLRLHATLPPSGTVIGRMSVKSITDKGAGKG 117 A +LGYPGFW ++PDTG DW +LLHGEQR+RLH LP SG V+G + +TDKG GKG Sbjct: 61 QAVVLGYPGFWAREPDTGIDWVKLLHGEQRMRLHRPLPASGEVVGHNRITHLTDKGEGKG 120 Query: 118 AIIVTERRLTDAVSGALLAEIEMLTFCRGDGGYSAGGQ--PSDSLDSAL-PSPPDTAPDA 174 AI+VTERRL A +G LLA ++ +TF RGDGGYS G PSD+ AL P+P D APD Sbjct: 121 AIMVTERRLETA-AGELLATVQQVTFLRGDGGYSQQGGGQPSDAPLPALQPTPQDRAPDF 179 Query: 175 VCDLPTRPESALIYRLSGDANPLHADPDVAATAGFERPILHGLATYGIAAHAILKTWCGY 234 PTRPE+AL+YRL GD NPLHADP VAA AGFERPILHGLA+YG+ AHA+L+ CG Sbjct: 180 TDTQPTRPEAALLYRLMGDYNPLHADPAVAAKAGFERPILHGLASYGLVAHALLRQCCGG 239 Query: 235 QPEQLTALDVRFSAPVYPGETIRTEMWR---QGDRIAFRARVLERDRIVLNNGMARL 288 P +L ALD+RF+APVYPGET+ TE+WR Q ++ RARVLERD++VL++G A L Sbjct: 240 DPARLKALDIRFAAPVYPGETLVTEIWRVPGQPTQVQLRARVLERDKVVLSHGFAEL 296