BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0118.p (356 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_001354128.1| PilW type IV pilus assembly protein [Janthin... 166 5e-39 ref|YP_001100629.1| putative Tfp pilus assembly protein PilW [He... 145 9e-33 ref|ZP_08273471.1| Type IV fimbrial biogenesis protein PilW [Oxa... 119 9e-25 ref|YP_004686536.1| type IV pilus assembly protein PilW [Cupriav... 117 2e-24 ref|YP_727282.1| Type IV pilus assembly protein PilW [Ralstonia ... 114 2e-23 >ref|YP_001354128.1| PilW type IV pilus assembly protein [Janthinobacterium sp. Marseille] gb|ABR89296.1| PilW type IV pilus assembly protein [Janthinobacterium sp. Marseille] Length = 305 Score = 166 bits (420), Expect = 5e-39, Method: Compositional matrix adjust. Identities = 108/267 (40%), Positives = 142/267 (53%), Gaps = 38/267 (14%) Query: 117 ARSVRQANYVPWDNPAFLRNDLGGLDPGIEGLDNARVGEHTVGMLSSSDNRAAHGSDLLM 176 +R++RQA Y D P I G+D + + ++ + +GSD++ Sbjct: 50 SRAIRQAGYENRDGDGTPIRRTADFSPDIMGMDARSLKRTGPALAEAAPSATVNGSDVMA 109 Query: 177 IRFFGSGPRNQPDNDMLNCAGFAVPGPNGASTLSDLEEERGQSIFYVALDRTGEPELRCK 236 +RFFG G ++NCAG VP A++ +E RG SIFYVA D GEPELRCK Sbjct: 110 LRFFGDG------EVIVNCAGLIVPA---AASQQHADEARGWSIFYVARDSGGEPELRCK 160 Query: 237 YLTRDGGWSAAAIVRGIESFQVLYGVRDVQNTGGDLDYVNATRIG--------------- 281 Y ++ G WSA AI RG+ESFQVLYGV DV G +VNA+ I Sbjct: 161 YRSQKG-WSADAIARGVESFQVLYGV-DVDGDGLADRFVNASDIDALDDAMPLTGENALA 218 Query: 282 --------TRWRDVAAVRIALLARGERNSRGGGTAVVHRLFGTAYQDGGDAGATIDETRL 333 T W+ + ++R++LL R E+ +RG VH LFG+AY D G IDE L Sbjct: 219 RAADKNRRTYWKRIRSIRVSLLMRSEQAARGDVPREVHYLFGSAYDRIDDKGTRIDEANL 278 Query: 334 APAVQQRIRKVFQTTIRLRN----SAP 356 +PAVQ+RIRKVF TI LRN SAP Sbjct: 279 SPAVQKRIRKVFTQTILLRNDIGGSAP 305 >ref|YP_001100629.1| putative Tfp pilus assembly protein PilW [Herminiimonas arsenicoxydans] emb|CAL62508.1| Putative Tfp pilus assembly protein PilW [Herminiimonas arsenicoxydans] Length = 315 Score = 145 bits (366), Expect = 9e-33, Method: Compositional matrix adjust. Identities = 97/265 (36%), Positives = 134/265 (50%), Gaps = 32/265 (12%) Query: 117 ARSVRQANYVPWDNPAFLRNDLGGLDPGIEGLDNARVGEHTVGMLSSSDNRAAHGSDLLM 176 +R+VRQA Y W + I G D + + T + S + +GSD+L Sbjct: 52 SRAVRQAGYENWHSATMPAQVEAAFSASITGRDAMTLKKTTPELDSLTSTDVVNGSDVLA 111 Query: 177 IRFFGSGPRNQPDNDMLNCAGFAVPGPNGASTLSDLEEERGQSIFYVALDRTGEPELRCK 236 +RFFG + + +LNCAG V + A+ E+ERG SIF+VA D GEPELRCK Sbjct: 112 LRFFGDDADGE-EGAILNCAGLPVAATSAAT---GNEQERGWSIFFVAKDSAGEPELRCK 167 Query: 237 YLTRDGGWSAAAIVRGIESFQVLYGVRDVQNTGGDLDYVNA------------------T 278 Y ++ W+A AI RG+ESFQVLYG+ D G ++NA Sbjct: 168 YQSKT-SWNADAIARGVESFQVLYGL-DADGDGVAEQFLNADGLNALDARLLLSGENALA 225 Query: 279 RIG-----TRWRDVAAVRIALLARGERNSRGGGTAVVHRLFGTAYQD---GGDAGATIDE 330 R G T W+ + +++ LL RG + +R + LFG AY D D G+ I E Sbjct: 226 RAGDFNRQTHWKKITVIKLGLLVRGSQVARDDALRGEYDLFGAAYGDMHAASDKGSRISE 285 Query: 331 TRLAPAVQQRIRKVFQTTIRLRNSA 355 + L A + RIRKVF TI+LRN+A Sbjct: 286 SALPAAAKNRIRKVFTHTIQLRNAA 310 >ref|ZP_08273471.1| Type IV fimbrial biogenesis protein PilW [Oxalobacteraceae bacterium IMCC9480] gb|EGF33058.1| Type IV fimbrial biogenesis protein PilW [Oxalobacteraceae bacterium IMCC9480] Length = 322 Score = 119 bits (297), Expect = 9e-25, Method: Compositional matrix adjust. Identities = 93/268 (34%), Positives = 128/268 (47%), Gaps = 48/268 (17%) Query: 118 RSVRQANYVPWDNPAFLRNDLGGLDPGIEGLDNARVGEHTVGMLSSSDNRAAHGSDLLMI 177 R+VRQ+ A L G + I GLD + T G+ + A +GSD+L + Sbjct: 66 RAVRQSASSDLARSAVLEQGNDGSEAAISGLDVRSLRAGTQGIEQALPG-AVNGSDVLAV 124 Query: 178 RFFGSGPRNQPDNDMLNCAGFAVPGPNGASTLSDLEEERGQSIFYVALDRTGEPELRCKY 237 RF G+ D +L+CAGF V P AS + G SIF+VA D GEPEL CKY Sbjct: 125 RFAGAD-----DGSVLDCAGFDVGIPATASP----GQLSGWSIFFVARDARGEPELHCKY 175 Query: 238 LTRDGGWSAAAIVRGIESFQVLYGVRDVQNTGGDL-----DYVNATRIG----------- 281 + WS+AAIVRG+E+FQVLYG+ G D+ ++ A++I Sbjct: 176 RGHNS-WSSAAIVRGVEAFQVLYGI------GPDMASLPTRFLTASQIDALDQQLALSGA 228 Query: 282 ------------TRWRDVAAVRIALLARGERNSRGGGTAVVHRLFGTAYQD---GGDAGA 326 T W+ + +RIALL R R R V LFG Y D D G Sbjct: 229 TPEQRAQDRNRQTHWKKITDIRIALLVRSMRPVRTDALDQVFDLFGAPYADQHAASDPGT 288 Query: 327 TIDETRLAPAVQQRIRKVFQTTIRLRNS 354 + E+ L + R+RK+F T+R+RN+ Sbjct: 289 RLVESALPAKDRNRLRKLFTATVRIRNA 316 >ref|YP_004686536.1| type IV pilus assembly protein PilW [Cupriavidus necator N-1] gb|AEI78055.1| type IV pilus assembly protein PilW [Cupriavidus necator N-1] Length = 344 Score = 117 bits (294), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 78/237 (32%), Positives = 121/237 (51%), Gaps = 31/237 (13%) Query: 141 LDPGIEGLDNARVGEHTVG-----MLSSSDNRAAHGSDLLMIRFFGSGP---RNQPDNDM 192 L P I GLD+ +G + + A + SD+L +RFFGSG R PDN M Sbjct: 109 LSPTISGLDSCGAPTQDLGNPAQNLACPAQAGAVNKSDVLQVRFFGSGTQQDRTVPDNSM 168 Query: 193 LNCAGFAVPGPNGASTLSDLEEERGQSIFYVALDRTGEPELRCKYLTRDG---------- 242 ++C+G V P D + RG ++FYV+ D +G P+L C Y RD Sbjct: 169 IDCSGNGVAEPQA---WEDADLARGMAVFYVSNDASGMPQLMCGYRPRDAANKEILTAVT 225 Query: 243 GWSAAAIVRGIESFQVLYGVRDVQNTGGDLDYVNATRI---GTRWRDVAAVRIALLARGE 299 + A +++ G+E FQVLYGV + D +++AT + T W++V A++IA++ G+ Sbjct: 226 AYQAVSLISGVEVFQVLYGVDTNFDQTPD-RFLSATEVLALPTTWQNVVAIKIAMVLMGD 284 Query: 300 RNSRGGGTAVVHRLFGTAYQDGGDAGATIDETRLAPAVQ-QRIRKVFQTTIRLRNSA 355 S RLFG + G+ ++T PA Q QR+R+++ T++LRN A Sbjct: 285 TASASPQDPPTFRLFGRLF-----PGSPGNDTSFTPATQLQRMRRLYTVTVQLRNMA 336 >ref|YP_727282.1| Type IV pilus assembly protein PilW [Ralstonia eutropha H16] emb|CAJ93914.1| Type IV pilus assembly protein PilW [Ralstonia eutropha H16] Length = 339 Score = 114 bits (285), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 77/237 (32%), Positives = 120/237 (50%), Gaps = 31/237 (13%) Query: 141 LDPGIEGLDNA-----RVGEHTVGMLSSSDNRAAHGSDLLMIRFFGSGP---RNQPDNDM 192 L P I GLD+ +G + + A + SD+L +RFFGSG R PDN M Sbjct: 104 LSPTISGLDSCGAPAQDLGNPAQNLACPAQAGAVNKSDVLQVRFFGSGTQQDRTVPDNSM 163 Query: 193 LNCAGFAVPGPNGASTLSDLEEERGQSIFYVALDRTGEPELRCKYLTRDG---------- 242 ++C+G V P D + RG ++FYV+ D G P+L C Y RD Sbjct: 164 IDCSGNGVAEPQA---WEDADLARGMAVFYVSNDAGGMPQLMCGYRPRDAANKEILTAGT 220 Query: 243 GWSAAAIVRGIESFQVLYGVRDVQNTGGDLDYVNATRI---GTRWRDVAAVRIALLARGE 299 + A +++ G+E FQVLYG+ + D +++AT + T W++V A++IA++ G+ Sbjct: 221 AYQAVSLISGVEVFQVLYGMDTNFDQTPD-RFLSATEVLALPTTWQNVVAIKIAMVLMGD 279 Query: 300 RNSRGGGTAVVHRLFGTAYQDGGDAGATIDETRLAPAVQ-QRIRKVFQTTIRLRNSA 355 S RLFG + G+ ++T PA Q QR+R+++ T++LRN A Sbjct: 280 TASASPQDPPTLRLFGRLF-----PGSPGNDTSFTPATQLQRMRRLYTVTVQLRNMA 331