BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0039.p (293 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_554966.1| putative spermidine synthase [Burkholderia xeno... 199 6e-49 ref|YP_001100787.1| putative transferase, spermidine synthase [H... 194 1e-47 ref|YP_004688516.1| spermidine synthase SpeE [Cupriavidus necato... 193 2e-47 ref|YP_726669.1| spermidine synthase [Ralstonia eutropha H16] >g... 191 1e-46 ref|YP_003847036.1| Spermine synthase [Gallionella capsiferrifor... 189 5e-46 >ref|YP_554966.1| putative spermidine synthase [Burkholderia xenovorans LB400] gb|ABE35616.1| Putative spermidine synthase [Burkholderia xenovorans LB400] Length = 277 Score = 199 bits (505), Expect = 6e-49, Method: Compositional matrix adjust. Identities = 107/228 (46%), Positives = 135/228 (59%), Gaps = 2/228 (0%) Query: 20 AFEEAYLASKGKPFFFDSDDMRT--LHFDARFIQSAMRLSDPDELLLSYTRGMMIFLLFN 77 A E G P SD +RT L F IQS+MR DP EL L YTR MM F LF+ Sbjct: 11 ALAELLKGPPGNPVPVVSDGLRTRSLRFGPLGIQSSMRKRDPFELDLPYTRAMMTFGLFH 70 Query: 78 PQPRSILMIGLGGGSLAKYCHRMFPDTNITVLEIDEDVIALREDFAVPPDSERFVVLHTD 137 P P+ IL++GLGGGSL+KYC R FP +T +EID +VIALRE F VPPDS RF ++H D Sbjct: 71 PAPQDILIVGLGGGSLSKYCFRKFPSARVTTVEIDANVIALRERFFVPPDSARFRIVHAD 130 Query: 138 AKDYIATMEAKVDVILHDGFDANGLVPALNTTAFYTTCRAALSENGILVSNFWGDDEDLA 197 A Y++ DVIL DGFDA GL AL++ +FY +CRAAL + G+LVSN +D L Sbjct: 131 AAAYLSDSTGIADVILLDGFDATGLPAALSSQSFYDSCRAALRDKGVLVSNLLNNDVRLT 190 Query: 198 SSMERLWSAFDSRLWWSTATASLNRVVCALHGNQDAALSDLQKQAVVL 245 + + R+ A D+ L +TA N + L A DL +A L Sbjct: 191 THLARMRRACDANLLRTTAQCGANVIALGLKRRTVPAWDDLHARAKAL 238 >ref|YP_001100787.1| putative transferase, spermidine synthase [Herminiimonas arsenicoxydans] emb|CAL62666.1| Putative spermidine synthase [Herminiimonas arsenicoxydans] Length = 233 Score = 194 bits (493), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 105/225 (46%), Positives = 134/225 (59%), Gaps = 4/225 (1%) Query: 40 MRTLHFDARFIQSAMRLSDPDELLLSYTRGMMIFLLFNPQPRSILMIGLGGGSLAKYCHR 99 MRTLHFD ++IQSAM +S PD+LLLSYTR MM FLLF P P+ ILM+GLGGGSL KYC+R Sbjct: 1 MRTLHFDHQYIQSAMSISAPDKLLLSYTRAMMGFLLFQPAPKHILMVGLGGGSLVKYCYR 60 Query: 100 MFPDTNITVLEIDEDVIALREDFAVPPDSERFVVLHTDAKDYIATM-EAKVDVILHDGFD 158 FP T ITVLE+ +VIALR+ F +P D +R ++ DA Y+ E DVI+ DGF Sbjct: 61 HFPSTRITVLELHAEVIALRDHFMIPADDQRLEIIQADAAVYMRDQPEGIADVIMLDGFS 120 Query: 159 ANGLVPALNTTAFYTTCRAALSENGILVSNFWGDDEDLASSMERLWSAFDSRLWWSTATA 218 A+G L T+ FY C L+ G+LVSN W +A ++ D R+WW Sbjct: 121 ADGPASELGTSDFYRACLHTLAPQGVLVSNLWDRPTSMAPAIADALLETDWRIWWCRTDD 180 Query: 219 SLNRVVCALHGNQDAAL--SDLQKQAVVLDHLYKLGFEGLAERFQ 261 S N + + GN DA + S L +A LD + L L FQ Sbjct: 181 SYNYIAFFVKGN-DAGVFRSMLFNRAQKLDQRFDLELAKLTPSFQ 224 >ref|YP_004688516.1| spermidine synthase SpeE [Cupriavidus necator N-1] gb|AEI82478.1| spermidine synthase SpeE [Cupriavidus necator N-1] Length = 288 Score = 193 bits (491), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 105/249 (42%), Positives = 141/249 (56%), Gaps = 7/249 (2%) Query: 23 EAYL------ASKGKPFFFDSDDMRTLHFDARFIQSAMRLSDPDELLLSYTRGMMIFLLF 76 EAYL S G P ++ +LHFDA QS M DPD+L+L YTR MM FLL Sbjct: 21 EAYLKFLAEATSDGTPQVLETKHALSLHFDALATQSFMSRKDPDKLVLGYTRTMMGFLLL 80 Query: 77 NPQPRSILMIGLGGGSLAKYCHRMFPDTNITVLEIDEDVIALREDFAVPPDSERFVVLHT 136 +P P I MIGLGGGSLAKYC+R P+T + +EI+ VIALRE F +PPD ER V+ Sbjct: 81 HPTPTRISMIGLGGGSLAKYCYRHLPNTKVVAIEINPAVIALREQFQIPPDDERLEVVCA 140 Query: 137 DAKDYIATMEAKVDVILHDGFDANGLVPALNTTAFYTTCRAALSENGILVSNFWGDDEDL 196 D +Y+A A+ DVIL DGF A+G+ L T FY C A L++ G+LV+NF D D+ Sbjct: 141 DGAEYVANQNARPDVILVDGFLADGMPVQLGTADFYAACHARLADTGVLVANFLESDPDI 200 Query: 197 ASSMERLWSAFDSRLWWSTATASLNRVVCALHGNQD-AALSDLQKQAVVLDHLYKLGFEG 255 +E + + F + S + S N + A G AL L+ +A +L+ + L Sbjct: 201 PLYLEEVSAVFGKSMSISLSEDSANYTLFAWKGAHKLPALEALKARARILETAHSLNLTA 260 Query: 256 LAERFQTGQ 264 A R + G+ Sbjct: 261 AAHRLKRGE 269 >ref|YP_726669.1| spermidine synthase [Ralstonia eutropha H16] emb|CAJ93301.1| spermidine synthase [Ralstonia eutropha H16] Length = 276 Score = 191 bits (485), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 102/236 (43%), Positives = 137/236 (58%), Gaps = 1/236 (0%) Query: 30 GKPFFFDSDDMRTLHFDARFIQSAMRLSDPDELLLSYTRGMMIFLLFNPQPRSILMIGLG 89 G P ++ +LHFDA QS M DPD+L+L YTR MM FLL +P P I MIGLG Sbjct: 22 GTPKVLETQHALSLHFDALATQSFMSRKDPDKLVLGYTRTMMGFLLLHPVPARISMIGLG 81 Query: 90 GGSLAKYCHRMFPDTNITVLEIDEDVIALREDFAVPPDSERFVVLHTDAKDYIATMEAKV 149 GGSLAKYC+R P+T I +EI+ VIALRE F +PPD ER V+ D DY+A +A+ Sbjct: 82 GGSLAKYCYRHLPNTKIVAIEINPAVIALREQFQIPPDDERLEVVCADGADYVANQDARP 141 Query: 150 DVILHDGFDANGLVPALNTTAFYTTCRAALSENGILVSNFWGDDEDLASSMERLWSAFDS 209 DVIL DGF A+G+ L T FY C A L++ G+LV+NF D D+ +E + +AF Sbjct: 142 DVILVDGFLADGMPVQLGTADFYAACHARLADTGVLVANFLESDPDIPLYLEEVSTAFGQ 201 Query: 210 RLWWSTATASLNRVVCALHGNQD-AALSDLQKQAVVLDHLYKLGFEGLAERFQTGQ 264 + S + S N + A G +L L +A +L+ + L A R + G+ Sbjct: 202 SMSISLSEDSSNYTLFAWKGAHKLPSLDALIARARILETAHSLSLTAAARRLKRGE 257 >ref|YP_003847036.1| Spermine synthase [Gallionella capsiferriformans ES-2] gb|ADL55272.1| Spermine synthase [Gallionella capsiferriformans ES-2] Length = 268 Score = 189 bits (479), Expect = 5e-46, Method: Compositional matrix adjust. Identities = 101/257 (39%), Positives = 147/257 (57%), Gaps = 1/257 (0%) Query: 5 KMKHTLLKSFQKKASAFEEAYLASKGKPFFFDSDDMRTLHFDARFIQSAMRLSDPDELLL 64 K K+ +K+ + + + A KP+ + D + L+FDA +QS M + PDEL++ Sbjct: 2 KFKNKHVKNIARTVCREFDRFCAGAAKPYLTERDGVLALNFDAMSVQSEMYVDRPDELVI 61 Query: 65 SYTRGMMIFLLFNPQPRSILMIGLGGGSLAKYCHRMFPDTNITVLEIDEDVIALREDFAV 124 SYTR MM FLL NP P I MIGLGGGS+AKYC+R I V+EI DVIALR DFA+ Sbjct: 62 SYTRAMMSFLLLNPAPERIAMIGLGGGSIAKYCYRNVTSAEIQVIEISPDVIALRNDFAI 121 Query: 125 PPDSERFVVLHTDAKDYIATMEAKVDVILHDGFDANGLVPALNTTAFYTTCRAALSENGI 184 P D RF VL D ++A + +V++ DGFD GL AL++ FY C AAL+++GI Sbjct: 122 PEDDARFCVLANDGARWVADTTFQPEVLIVDGFDMEGLPAALSSQRFYDDCFAALADSGI 181 Query: 185 LVSNFWGDDEDLASSMERLWSAFDSRLWWSTATASLNRVVCAL-HGNQDAALSDLQKQAV 243 L N WG + R+ ++F R+ + N++V A+ +G +S +++ A Sbjct: 182 LAVNLWGGYPHYEDYLARISNSFAGRIVVVDSDDEFNKIVLAVKNGVFPPDISFIRQHAN 241 Query: 244 VLDHLYKLGFEGLAERF 260 VLD + L F+ + R Sbjct: 242 VLDMSHPLNFQSKSNRL 258