BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0004.p (509 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_004751133.1| putative transport protein (MFS family) [Col... 613 e-173 ref|YP_001355030.1| major facilitator superfamily multidrug-effl... 592 e-167 ref|YP_001101327.1| MFS family transporter [Herminiimonas arseni... 590 e-166 ref|YP_003777341.1| major facilitator superfamily permease [Herb... 582 e-164 gb|EGW61920.1| major facilitator superfamily MFS_1 [Dechlorosoma... 436 e-120 >ref|YP_004751133.1| putative transport protein (MFS family) [Collimonas fungivorans Ter331] gb|AEK60310.1| putative transport protein (MFS family) [Collimonas fungivorans Ter331] Length = 406 Score = 613 bits (1580), Expect = e-173, Method: Compositional matrix adjust. Identities = 310/397 (78%), Positives = 352/397 (88%) Query: 15 ESAQKAGMSGAEVRASASLASIFALRMLGLFLILPVFAVHAKGMSGGESAAMVGLAMGIY 74 S +GMS AEV+ASASLASIFALRMLGLFLILPVFAVHAK + GG+SAA+VGLAMGIY Sbjct: 2 SSEISSGMSRAEVKASASLASIFALRMLGLFLILPVFAVHAKTLPGGDSAALVGLAMGIY 61 Query: 75 GLAQSFGQIPFGMASDKYGRKRVIVVGLVLFALGSFVAAGAQDIGWVIAGRAIQGAGAIS 134 GLAQSFGQIPFG+ASDKYGRK++IV+GL+LFALGSF+AA A +I WVI GRAIQGAGAIS Sbjct: 62 GLAQSFGQIPFGVASDKYGRKKIIVIGLILFALGSFIAAAAGNIYWVIIGRAIQGAGAIS 121 Query: 135 AAVTAFIADSTREEHRTKAMAMVGGSIGLTFALSLVASPLLYEAIGMGGIFGLTGVLALA 194 AAVTAFIADSTREEHRTKAMAMVGGSIGLTFALSL+ SPLLY+ +GMGGIF LTG+L+LA Sbjct: 122 AAVTAFIADSTREEHRTKAMAMVGGSIGLTFALSLIVSPLLYQWVGMGGIFALTGILSLA 181 Query: 195 AIGVVLFVVPDAPMVKARRVPLGEVLRHGELMRLNFGVFALHMTQMAMFVVLPAALVQYA 254 AI VV+++VP AP+VKARRV E+LR+GELMRLN+GVFALHMTQMAMFVV+PAALV+YA Sbjct: 182 AIVVVVWLVPSAPLVKARRVAWSEILRNGELMRLNYGVFALHMTQMAMFVVMPAALVKYA 241 Query: 255 GLPVAEHWKIYLPVVLASFIFMLPPVFIGEKRGLMKQVFVAAISLLLLVQVGMWATLAHF 314 GLP+ HWKIYLPVVLASF+ MLPPVF+GEK+G MKQV +AA++LL +VQ+G+ AT + Sbjct: 242 GLPLESHWKIYLPVVLASFVLMLPPVFVGEKQGKMKQVMLAAVALLFVVQLGLLATFSMA 301 Query: 315 AAHWWVLVFLLFAFFIAFNILEACQPSLVSRIAPAAAKGTALGVYNTLQALGLFCGGALG 374 HW LV LL FFIAFN+LEA QPSLVSRIAP AAKG ALGVYNT+QALGLFCGGALG Sbjct: 302 TIHWQWLVVLLLGFFIAFNVLEASQPSLVSRIAPPAAKGAALGVYNTMQALGLFCGGALG 361 Query: 375 GWLKQFAGAPSVFVMCAVATLTWLIIASNMKNLPRRG 411 G LKQ AGAPSVF++ ATL WLIIAS+MKNLPRRG Sbjct: 362 GLLKQHAGAPSVFILGGAATLCWLIIASSMKNLPRRG 398 >ref|YP_001355030.1| major facilitator superfamily multidrug-efflux transporter [Janthinobacterium sp. Marseille] gb|ABR91041.1| multidrug-efflux transporter of the MFS superfamily [Janthinobacterium sp. Marseille] Length = 409 Score = 592 bits (1525), Expect = e-167, Method: Compositional matrix adjust. Identities = 297/391 (75%), Positives = 338/391 (86%) Query: 21 GMSGAEVRASASLASIFALRMLGLFLILPVFAVHAKGMSGGESAAMVGLAMGIYGLAQSF 80 GM+ E+RAS SLASIFALRMLGLFLILPVFAVHAK + GGESA +VGLAMGIYGL QSF Sbjct: 12 GMTRTEIRASTSLASIFALRMLGLFLILPVFAVHAKTLPGGESATLVGLAMGIYGLTQSF 71 Query: 81 GQIPFGMASDKYGRKRVIVVGLVLFALGSFVAAGAQDIGWVIAGRAIQGAGAISAAVTAF 140 GQIPFG+ASDKYGRKR+IV+GL+LFALGSF+AA A +I WVI GR++QGAGAISAAVTAF Sbjct: 72 GQIPFGIASDKYGRKRIIVIGLILFALGSFIAAAADNIIWVIIGRSVQGAGAISAAVTAF 131 Query: 141 IADSTREEHRTKAMAMVGGSIGLTFALSLVASPLLYEAIGMGGIFGLTGVLALAAIGVVL 200 IADSTREEHRTKAMAMVGGSIGLTFA SL+ SPLLY+ IGMGGIF LTG+L++ AI VV+ Sbjct: 132 IADSTREEHRTKAMAMVGGSIGLTFAGSLILSPLLYQWIGMGGIFALTGILSVLAIFVVI 191 Query: 201 FVVPDAPMVKARRVPLGEVLRHGELMRLNFGVFALHMTQMAMFVVLPAALVQYAGLPVAE 260 +VVP AP + +V + E+L HGELMRLNFGVFALH+TQMAMFVV+P+AL+++A LP+AE Sbjct: 192 YVVPAAPSLPPAQVSIREILAHGELMRLNFGVFALHLTQMAMFVVVPSALIRFAQLPLAE 251 Query: 261 HWKIYLPVVLASFIFMLPPVFIGEKRGLMKQVFVAAISLLLLVQVGMWATLAHFAAHWWV 320 HWKIYLPVV+ASF+ MLPP+FIGEKRG KQVFVAAI+LLLLVQVGMW L+ HW Sbjct: 252 HWKIYLPVVIASFVLMLPPIFIGEKRGKSKQVFVAAIALLLLVQVGMWLVLSQTQVHWTW 311 Query: 321 LVFLLFAFFIAFNILEACQPSLVSRIAPAAAKGTALGVYNTLQALGLFCGGALGGWLKQF 380 LV LL AFF+AFNILEA QPSLVSRIAP +KG ALGVYNTLQALGLFCGGALGGWL Q Sbjct: 312 LVGLLLAFFVAFNILEASQPSLVSRIAPPGSKGAALGVYNTLQALGLFCGGALGGWLTQH 371 Query: 381 AGAPSVFVMCAVATLTWLIIASNMKNLPRRG 411 G SVFV+ + + WLIIA+NMKNLPRRG Sbjct: 372 VGGASVFVLGSALCVVWLIIAANMKNLPRRG 402 >ref|YP_001101327.1| MFS family transporter [Herminiimonas arsenicoxydans] emb|CAL63206.1| putative Permease of the major facilitator superfamily [Herminiimonas arsenicoxydans] Length = 397 Score = 590 bits (1521), Expect = e-166, Method: Compositional matrix adjust. Identities = 300/390 (76%), Positives = 335/390 (85%) Query: 22 MSGAEVRASASLASIFALRMLGLFLILPVFAVHAKGMSGGESAAMVGLAMGIYGLAQSFG 81 M+ E+RAS SLASIFALRMLGLFLILPVF+VHAKG+ GGESA +VGLA+GIYGL QSFG Sbjct: 1 MTSNEIRASTSLASIFALRMLGLFLILPVFSVHAKGLPGGESATLVGLALGIYGLTQSFG 60 Query: 82 QIPFGMASDKYGRKRVIVVGLVLFALGSFVAAGAQDIGWVIAGRAIQGAGAISAAVTAFI 141 QIPFG+ASDKYGRKRVIV+GL+LFALGSF+AA A DI WVI GRA+QGAGAISAAVTAFI Sbjct: 61 QIPFGIASDKYGRKRVIVIGLILFALGSFIAAAADDIVWVIVGRAVQGAGAISAAVTAFI 120 Query: 142 ADSTREEHRTKAMAMVGGSIGLTFALSLVASPLLYEAIGMGGIFGLTGVLALAAIGVVLF 201 ADSTREEHRTKAMAMVG SIG+TFA SLV SPLLY+AIGMGGIF LTG+L++ AI VVL+ Sbjct: 121 ADSTREEHRTKAMAMVGASIGVTFAGSLVLSPLLYQAIGMGGIFALTGILSVLAILVVLY 180 Query: 202 VVPDAPMVKARRVPLGEVLRHGELMRLNFGVFALHMTQMAMFVVLPAALVQYAGLPVAEH 261 +VP AP + RV + EVL +GELMRLN+GVFALH+TQMAMFVV+P+AL+++ LP+ EH Sbjct: 181 IVPAAPALPPGRVSIREVLANGELMRLNYGVFALHVTQMAMFVVMPSALIRFGHLPLGEH 240 Query: 262 WKIYLPVVLASFIFMLPPVFIGEKRGLMKQVFVAAISLLLLVQVGMWATLAHFAAHWWVL 321 WKIYLPVVLASFI MLPP+FI E+RG KQVFVAAI+LLL VQVGMW A HW L Sbjct: 241 WKIYLPVVLASFILMLPPIFIAERRGKSKQVFVAAIALLLAVQVGMWLVFAQTQLHWAWL 300 Query: 322 VFLLFAFFIAFNILEACQPSLVSRIAPAAAKGTALGVYNTLQALGLFCGGALGGWLKQFA 381 V LL FFIAFNILEA QPSLVSRIAP AAKG ALGVYNTLQALGLFCGGALGGWL Q Sbjct: 301 VALLLVFFIAFNILEASQPSLVSRIAPPAAKGAALGVYNTLQALGLFCGGALGGWLTQHT 360 Query: 382 GAPSVFVMCAVATLTWLIIASNMKNLPRRG 411 G SVFV+ AV T+ WLIIA+NMKNLPRRG Sbjct: 361 GGASVFVLGAVLTVAWLIIAANMKNLPRRG 390 >ref|YP_003777341.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] gb|ADJ65433.1| permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 398 Score = 582 bits (1501), Expect = e-164, Method: Compositional matrix adjust. Identities = 300/398 (75%), Positives = 347/398 (87%) Query: 22 MSGAEVRASASLASIFALRMLGLFLILPVFAVHAKGMSGGESAAMVGLAMGIYGLAQSFG 81 MS AEVRASASLASIF+LRMLGLFL+LPVFAVHA + GG++ +VG+A+GIYGLAQ+FG Sbjct: 1 MSRAEVRASASLASIFSLRMLGLFLVLPVFAVHAHSLPGGDNDTLVGIALGIYGLAQAFG 60 Query: 82 QIPFGMASDKYGRKRVIVVGLVLFALGSFVAAGAQDIGWVIAGRAIQGAGAISAAVTAFI 141 QIP+G+ASD+YGRKR+IV+GL+LFALGSFVAA A + W++ GRA+QG+GAISAA+TAFI Sbjct: 61 QIPYGIASDRYGRKRIIVIGLLLFALGSFVAAAATSVIWLLVGRALQGSGAISAAITAFI 120 Query: 142 ADSTREEHRTKAMAMVGGSIGLTFALSLVASPLLYEAIGMGGIFGLTGVLALAAIGVVLF 201 ADSTREEHRTKAMAMVG SIG+TFA+S+VASPLLY++IGMGGIFGLTGVL+L AI VV++ Sbjct: 121 ADSTREEHRTKAMAMVGASIGVTFAVSMVASPLLYKSIGMGGIFGLTGVLSLIAIAVVVW 180 Query: 202 VVPDAPMVKARRVPLGEVLRHGELMRLNFGVFALHMTQMAMFVVLPAALVQYAGLPVAEH 261 VVP AP+V+ARRVPL EVLRHGELMRLNFGVF LH+TQ+AMF+V+P ALVQ AGLP+ EH Sbjct: 181 VVPAAPLVQARRVPLREVLRHGELMRLNFGVFTLHLTQVAMFMVVPGALVQLAGLPLGEH 240 Query: 262 WKIYLPVVLASFIFMLPPVFIGEKRGLMKQVFVAAISLLLLVQVGMWATLAHFAAHWWVL 321 WKIYLPVVLASF+ MLPPVF+ EK G MKQVF+ AI L+LLVQ+G WA L+H HWW+L Sbjct: 241 WKIYLPVVLASFVLMLPPVFVAEKYGRMKQVFLGAIGLMLLVQLGFWAVLSHPLGHWWML 300 Query: 322 VFLLFAFFIAFNILEACQPSLVSRIAPAAAKGTALGVYNTLQALGLFCGGALGGWLKQFA 381 V LLF FF+AFNILEA QPSLVSRIAP AAKG ALGVYNTLQALGL CGGALGGW+KQ Sbjct: 301 VALLFLFFVAFNILEASQPSLVSRIAPPAAKGAALGVYNTLQALGLSCGGALGGWIKQNV 360 Query: 382 GAPSVFVMCAVATLTWLIIASNMKNLPRRGAQXRGRPA 419 G VF + AV TLTWLIIA+NMKNLPRR A G A Sbjct: 361 GISEVFALSAVVTLTWLIIAANMKNLPRRSAPAAGATA 398 >gb|EGW61920.1| major facilitator superfamily MFS_1 [Dechlorosoma suillum PS] Length = 449 Score = 436 bits (1121), Expect = e-120, Method: Compositional matrix adjust. Identities = 244/400 (61%), Positives = 288/400 (72%), Gaps = 10/400 (2%) Query: 22 MSGAEVRASASLASIFALRMLGLFLILPVFAVHAKGMSGGESAAMVGLAMGIYGLAQSFG 81 M+ E RA ASLASIFALRMLGLFLILPVFAV+A + G++ +VGLA+GIYG Q+ Sbjct: 58 MTPEERRAGASLASIFALRMLGLFLILPVFAVYAHTLPSGDNGLLVGLAIGIYGATQALF 117 Query: 82 QIPFGMASDKYGRKRVIVVGLVLFALGSFVAAGAQDIGWVIAGRAIQGAGAISAAVTAFI 141 QIP+G+ASD++GRK VIV GL+LFALGSF+AA A D+ +IAGR +QGAGAISAAVTA Sbjct: 118 QIPYGIASDRFGRKPVIVFGLLLFALGSFIAAAADDLPMIIAGRVLQGAGAISAAVTALA 177 Query: 142 ADSTREEHRTKAMAMVGGSIGLTFALSLVASPLLYEAIGMGGIFGLTGVLALAAIGVVLF 201 AD TRE+HRTK MAM+G SIGL FALSLVA+PLLY AIGM GIF LTG LAL AI + Sbjct: 178 ADLTREQHRTKVMAMIGSSIGLVFALSLVAAPLLYAAIGMAGIFALTGGLALLAIVALYK 237 Query: 202 VVPDAP--MVKARRVPLGEVLRHGELMRLNFGVFALHMTQMAMFVVLPAALVQYAGLPVA 259 VVP AP +V R +VL H +L+RLNFGVF LH+TQ AM+V++PAALV+ LP+A Sbjct: 238 VVPPAPKVLVDGGRTRFADVLFHPQLLRLNFGVFVLHLTQTAMWVLIPAALVKTGDLPLA 297 Query: 260 EHWKIYLPVVLASFIFMLPPVFIGEKRGLMKQVFVAAISLLLLVQVGMWATLAHFAAHWW 319 EHWK+YLP VL SF FM+P V EK G MK VF A+ LLLLVQ G F W Sbjct: 298 EHWKVYLPAVLVSFAFMVPAVIAAEKYGRMKMVFNGAVVLLLLVQAG----FGLFGQGLW 353 Query: 320 VLVFLLFAFFIAFNILEACQPSLVSRIAPAAAKGTALGVYNTLQALGLFCGGALGGWLKQ 379 L LL AFF+AFNILEA QPSL+SRIAP AKG ALG+YNT Q+LGLF GGA+GG L Q Sbjct: 354 PLALLLTAFFVAFNILEATQPSLISRIAPVEAKGAALGIYNTTQSLGLFLGGAVGGMLAQ 413 Query: 380 FAGAPSVFVMCAVATLTWLIIASNMKNLPRRGAQXRGRPA 419 G +V+ + +L WL + MK P R Q RPA Sbjct: 414 RLGGDAVWWCTGILSLVWLALGLTMKP-PVRAQQ---RPA 449