BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0205.p (454 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003774605.1| small-conductance mechanosensitive channel p... 541 e-151 ref|YP_004751885.1| putative mechanosensitive ion channel protei... 476 e-132 ref|YP_001354258.1| mechanosensitive ion channel protein [Janthi... 394 e-107 ref|YP_001100722.1| putative mechanosensitive ion channel protei... 372 e-101 gb|EGW59606.1| MscS Mechanosensitive ion channel [Dechlorosoma s... 301 1e-79 >ref|YP_003774605.1| small-conductance mechanosensitive channel protein [Herbaspirillum seropedicae SmR1] gb|ADJ62697.1| small-conductance mechanosensitive channel protein [Herbaspirillum seropedicae SmR1] Length = 358 Score = 541 bits (1393), Expect = e-151, Method: Compositional matrix adjust. Identities = 265/356 (74%), Positives = 300/356 (84%), Gaps = 4/356 (1%) Query: 97 MMPLMISQVAMRFVFYVLRRIFVKETTAGAFLQLFEKVFATLVWICVLLYITGMWPDLVD 156 M PL ++QVAMRFVFYVLR IFVK+ T GA L L EK A LVW+CV+L+ITG+WPD VD Sbjct: 1 MFPLTVAQVAMRFVFYVLRGIFVKDATVGALLHLSEKAIAVLVWLCVVLWITGLWPDFVD 60 Query: 157 YLDTTMLPLGRHKTSLLTILQATASVLVTLIIALWAGAMLEDRMMKLNTMHLSLRTVVAR 216 YLD T LPL RHK SLLT+LQ ASVLVTLIIALW G +LE+R+MK + MH SLRTVVAR Sbjct: 61 YLDGTTLPLARHKVSLLTMLQGAASVLVTLIIALWIGTVLENRLMKFSGMHSSLRTVVAR 120 Query: 217 MVRAILILVAILVSLSAVGIDLTVLSVFGGALGVGLGLGLQKIASSYVSGFVILLERSLA 276 M+RA+LIL+A LVSLS VGIDLTVLSVFGGALGVG+GLGLQKIASSYVSGFVILLERS+ Sbjct: 121 MLRALLILIAFLVSLSLVGIDLTVLSVFGGALGVGIGLGLQKIASSYVSGFVILLERSMV 180 Query: 277 IGDMVNVDRYFGKVTQINTRYTILEGLDGIESVLPNEMFMSTPVQNYSLNRKIIRLATQV 336 IGDMV VD+YFGKVTQINTRYTILEGLDGIESVLPNE+F+S PVQNYSL +I+RL+TQ+ Sbjct: 181 IGDMVAVDKYFGKVTQINTRYTILEGLDGIESVLPNELFVSNPVQNYSLTHRIVRLSTQL 240 Query: 337 TILYQDDIETVLSMLEKAAFAVERVSDQILPQALLLKIGEDGLQLEIGFWITDPENGRLN 396 TILYQDD+ETVLS++E+AA V+RVS Q PQALL+KIG DGL LE+GFWITDPENGRLN Sbjct: 241 TILYQDDVETVLSIMEQAALGVQRVSQQTAPQALLIKIGADGLDLELGFWITDPENGRLN 300 Query: 397 VLSDVNRAIWRVFKTHGIQVAHPKRDIRLMDPRSFEQSVDDMTSSAHKDAQSAYNA 452 VLSDVNRAIW FK HGI+VAH KRDIR+MD RSF QS T + D N+ Sbjct: 301 VLSDVNRAIWAAFKQHGIEVAHAKRDIRIMDERSFRQS----TGKENPDMPGGQNS 352 >ref|YP_004751885.1| putative mechanosensitive ion channel protein [Collimonas fungivorans Ter331] gb|AEK61062.1| Putative mechanosensitive ion channel protein [Collimonas fungivorans Ter331] Length = 457 Score = 476 bits (1226), Expect = e-132, Method: Compositional matrix adjust. Identities = 256/402 (63%), Positives = 322/402 (80%), Gaps = 1/402 (0%) Query: 39 SRWLRKLFTLSGGDEQSVVHIGVKSFVRVLSPLLILILLTLARLVLIRLKLPVTLWHVMM 98 +R LR+ F++S + + IG+ SF +VL+P++ L+LL L +L L + + PV + +M+ Sbjct: 39 ARLLRRSFSVSTA-QPPAMQIGLGSFSKVLTPIITLLLLILVKLALQKWQQPVNVMRLMI 97 Query: 99 PLMISQVAMRFVFYVLRRIFVKETTAGAFLQLFEKVFATLVWICVLLYITGMWPDLVDYL 158 PL+ S MRFVFYVLRR+F + + G FL LFEK F LVWI +LLYITG WPDL DYL Sbjct: 98 PLVASLALMRFVFYVLRRVFARNSGVGTFLLLFEKSFGVLVWIGLLLYITGWWPDLFDYL 157 Query: 159 DTTMLPLGRHKTSLLTILQATASVLVTLIIALWAGAMLEDRMMKLNTMHLSLRTVVARMV 218 D+T+LP+GR+K SLL I+QA SVLVTL+IALWAGA LE+R+M++NT+H S+RTVVARM Sbjct: 158 DSTVLPIGRYKVSLLAIMQALVSVLVTLVIALWAGAALEERLMRVNTVHSSVRTVVARMA 217 Query: 219 RAILILVAILVSLSAVGIDLTVLSVFGGALGVGLGLGLQKIASSYVSGFVILLERSLAIG 278 RA LILVA+L+SLS VGIDLTVLSVFGGALGV LGLGLQKIASSYVSGFVILLERSLAIG Sbjct: 218 RAFLILVAVLLSLSLVGIDLTVLSVFGGALGVALGLGLQKIASSYVSGFVILLERSLAIG 277 Query: 279 DMVNVDRYFGKVTQINTRYTILEGLDGIESVLPNEMFMSTPVQNYSLNRKIIRLATQVTI 338 DMV V ++GKV QIN+RYT+L+GLDG+++V+PNE+FM VQNYSL +I+RLAT VTI Sbjct: 278 DMVGVSTFYGKVVQINSRYTVLQGLDGVDTVVPNEVFMINSVQNYSLTNRILRLATHVTI 337 Query: 339 LYQDDIETVLSMLEKAAFAVERVSDQILPQALLLKIGEDGLQLEIGFWITDPENGRLNVL 398 +QDD+E++L +LE+ V+RVS +ILPQALL+KIG DGL+LE+GFWITDPENGRLNVL Sbjct: 338 EHQDDVESILLLLEQVTVEVDRVSREILPQALLMKIGPDGLELEVGFWITDPENGRLNVL 397 Query: 399 SDVNRAIWRVFKTHGIQVAHPKRDIRLMDPRSFEQSVDDMTS 440 SDVNRAIWRV ++ I+VA KRDIR++ +S D S Sbjct: 398 SDVNRAIWRVLQSRQIKVAQVKRDIRMVSEGELHKSNFDAYS 439 >ref|YP_001354258.1| mechanosensitive ion channel protein [Janthinobacterium sp. Marseille] gb|ABR89482.1| mechanosensitive ion channel protein (mscS family) [Janthinobacterium sp. Marseille] Length = 454 Score = 394 bits (1011), Expect = e-107, Method: Compositional matrix adjust. Identities = 227/434 (52%), Positives = 316/434 (72%), Gaps = 11/434 (2%) Query: 1 MSTLLTDFYDYLFGELPPTELLRQLAIIALCVACGWGLSRWLRKLFTLSGGDEQSVVHIG 60 +STL TD L+ +L L QL + LC A WG++R +R L + ++ +G Sbjct: 6 LSTLWTD----LWTDLGIPGLRWQLIAMVLCFALAWGIARIIRNLLATQEPQQNALRRLG 61 Query: 61 VKSFVRVLSPLLILILLTLARLVLIRLK--LPVTLWHVMMPLMISQVAMRFVFYVLRRIF 118 V+SF RVL PLL +LL +A+ +L RL V + V+MPL+ S +R FYV+RR+F Sbjct: 62 VESFTRVLWPLLTWLLLAIAKAILERLDGVDGVDVLRVVMPLVASFALIRLAFYVMRRVF 121 Query: 119 VKETTAGAFLQLFEKVFATLVWICVLLYITGMWPDLVDYLDTTMLPLGRHKTSLLTILQA 178 + AF+ LFE+ FA+LVWI V L+ITG PD++++LD T +PLG+HK S++T+LQA Sbjct: 122 ARGGKTSAFVLLFERSFASLVWIVVALHITGFLPDIIEFLDDTFVPLGKHKVSMMTMLQA 181 Query: 179 TASVLVTLIIALWAGAMLEDRMMKLNTMHLSLRTVVARMVRAILILVAILVSLSAVGIDL 238 SV VTLIIALWA AM+E+R+M+L+ MH S+R V++RM RA+LIL+A+LVSLS VGIDL Sbjct: 182 VVSVGVTLIIALWASAMIEERLMRLDGMHSSMRAVLSRMSRAVLILIALLVSLSMVGIDL 241 Query: 239 TVLSVFGGALGVGLGLGLQKIASSYVSGFVILLERSLAIGDMVNVDRYFGKVTQINTRYT 298 TVLSVFGGALGVGLGLGLQKIASSYVSGF+ILLERS+ IGD V +D++ G+V QINTRYT Sbjct: 242 TVLSVFGGALGVGLGLGLQKIASSYVSGFIILLERSMTIGDTVTMDKFTGRVAQINTRYT 301 Query: 299 ILEGLDGIESVLPNEMFMSTPVQNYSLNRKIIRLATQVTILYQDDIETVLSMLEKAAFAV 358 +L G DG +++LPN++F+S VQN SL+ +++L T++++ Y+ DI+ L +LE+AA +V Sbjct: 302 VLRGGDGSDTILPNDLFVSNAVQNLSLSDTVVQLTTRISVDYRTDIDLALRLLEEAALSV 361 Query: 359 ERV---SDQILPQALLLKIGEDGLQLEIGFWITDPENGRLNVLSDVNRAIWRVFKTHGIQ 415 E+V D++ P A ++ G DG+ L++GFW+ D + GR VLSDVNRAIWR F+ + I Sbjct: 362 EKVRKGPDRV-PGATMMAFGADGIDLQLGFWVNDLD-GRGTVLSDVNRAIWRSFQANEIN 419 Query: 416 VAHPKRDIRLMDPR 429 V P+R+IRL+D + Sbjct: 420 VPFPQREIRLIDEQ 433 >ref|YP_001100722.1| putative mechanosensitive ion channel protein [Herminiimonas arsenicoxydans] emb|CAL62601.1| Putative mechanosensitive ion channel protein [Herminiimonas arsenicoxydans] Length = 451 Score = 372 bits (954), Expect = e-101, Method: Compositional matrix adjust. Identities = 218/427 (51%), Positives = 306/427 (71%), Gaps = 6/427 (1%) Query: 24 QLAIIALCVACGWGLSRWLRKLFTLSGGDEQSVVHIGVKSFVRVLSPLLILILLTLARLV 83 Q+ + LC + GW L+R + ++ G + F RV P L L+L+ +A+ V Sbjct: 25 QILALVLCFSVGWLLARLGSGVLAAHDAQRKNAPRPGAEKFARVWWPFLTLVLIGIAKPV 84 Query: 84 LIRLKLPVTLWHVMMPLMISQVAMRFVFYVLRRIFVKETTAGAFLQLFEKVFATLVWICV 143 L R + V L + +PL+ S V +R FY+LRR+F ++ +F+ LF + FA LVW V Sbjct: 85 LARW-ISVDLLRLALPLVASFVLVRLAFYILRRVFARDGKGRSFILLFGRGFAGLVWTIV 143 Query: 144 LLYITGMWPDLVDYLDTTMLPLGRHKTSLLTILQATASVLVTLIIALWAGAMLEDRMMKL 203 L+ITG+ PD+ +LD T++PLGR+K SL+T+LQA SV VTL++ALW A+ E+R+MKL Sbjct: 144 ALHITGVLPDIAQFLDDTLIPLGRNKVSLMTMLQAALSVGVTLLVALWLSALAEERLMKL 203 Query: 204 NTMHLSLRTVVARMVRAILILVAILVSLSAVGIDLTVLSVFGGALGVGLGLGLQKIASSY 263 N+MH S+R V++RM RA+LIL+A+L+SLS VGIDLTVLSVFGGALGVGLGLGLQK+ASSY Sbjct: 204 NSMHSSMRAVLSRMGRALLILIALLLSLSMVGIDLTVLSVFGGALGVGLGLGLQKLASSY 263 Query: 264 VSGFVILLERSLAIGDMVNVDRYFGKVTQINTRYTILEGLDGIESVLPNEMFMSTPVQNY 323 VSGFVILLERSLAIGD V VD++ G+V+QINTRYT+L G DG ++V+PNEMF+S+PVQN Sbjct: 264 VSGFVILLERSLAIGDTVIVDKFTGRVSQINTRYTVLRGGDGTDTVVPNEMFVSSPVQNL 323 Query: 324 SLNRKIIRLATQVTILYQDDIETVLSMLEKAAFAVERV--SDQILPQALLLKIGEDGLQL 381 +L ++++ T++T+ Y+ DI+ L +LE+AA +VERV P A+L+ G DG+ L Sbjct: 324 TLTDTLVQMTTRITVDYRTDIDLALRLLEEAALSVERVRPGPDRGPGAILVAFGADGIDL 383 Query: 382 EIGFWITDPENGRLNVLSDVNRAIWRVFKTHGIQVAHPKRDIRLMDP--RSFEQSVDDMT 439 ++GFW+ DPE GR VLS+VNRAIWR F+ H I V P+R+IRL+D R+ ++ ++ T Sbjct: 384 QLGFWVNDPE-GRGGVLSNVNRAIWRSFQAHDINVPFPQREIRLIDEQYRAVKEHLNTNT 442 Query: 440 SSAHKDA 446 + D+ Sbjct: 443 RRSTPDS 449 >gb|EGW59606.1| MscS Mechanosensitive ion channel [Dechlorosoma suillum PS] Length = 431 Score = 301 bits (772), Expect = 1e-79, Method: Compositional matrix adjust. Identities = 169/427 (39%), Positives = 256/427 (59%), Gaps = 14/427 (3%) Query: 1 MSTLLTDFYDYLFGELPPTELLRQLAIIALCVACGWGLSRWLRKLFTLSGGDEQSVVHIG 60 + L TD +D +L + Q+A++ C+ + SR +R + H G Sbjct: 4 LPQLFTDLWD----DLQNPRIFWQMAVLLGCIGIAFFASRQVRNRIQTT----PERWHAG 55 Query: 61 VKSFVRVLSPLLILILLTLARLVLIRLKLPVTLWHVMMPLMISQVAMRFVFYVLRRIFVK 120 R+L PLL L+ LAR L++ + V L + +PLM+S +R + Y+LR+ F Sbjct: 56 RSGVKRLLFPLLAAGLVVLAR-ALLKPHMHVNLLTLAVPLMLSLAGIRLMVYILRQAFAP 114 Query: 121 ETTAGAFLQLFEKVFATLVWICVLLYITGMWPDLVDYLDTTMLPLGRHKTSLLTILQATA 180 +L E+V ATLVW+ L+ITG+ ++D ++ +G+ + L IL Sbjct: 115 S----GWLAASERVIATLVWLAAALHITGLDLPVIDAMEQVQFAVGKQRLDLWMILHGLV 170 Query: 181 SVLVTLIIALWAGAMLEDRMMKLNTMHLSLRTVVARMVRAILILVAILVSLSAVGIDLTV 240 ++ VT++ ALW + E R+M + ++R V+AR+++A+L LVA+L+SLS VGID+T Sbjct: 171 TIFVTVLGALWLAGLAEARLMGAQKLDSNVRAVLARIIKALLTLVAVLISLSLVGIDITT 230 Query: 241 LSVFGGALGVGLGLGLQKIASSYVSGFVILLERSLAIGDMVNVDRYF-GKVTQINTRYTI 299 LSVFGGALGVGLG GLQKIAS+Y+SGF+ILL+RS+ +GD + +D G VTQI TRYT+ Sbjct: 231 LSVFGGALGVGLGFGLQKIASNYISGFIILLDRSIRLGDFIAIDAATSGVVTQITTRYTV 290 Query: 300 LEGLDGIESVLPNEMFMSTPVQNYSLNRKIIRLATQVTILYQDDIETVLSMLEKAAFAVE 359 L GL G E ++PNE F++ VQN S + L T V + Y D+E +++L +AA A Sbjct: 291 LRGLAGTEYLIPNEQFVANVVQNQSYTDTQVFLKTAVQVGYNSDVERAMAVLVEAAAAHP 350 Query: 360 RVSDQILPQALLLKIGEDGLQLEIGFWITDPENGRLNVLSDVNRAIWRVFKTHGIQVAHP 419 RV + P+A L + G+ LE+GFWI DPE G + SD+N IW+ FK GI++ P Sbjct: 351 RVLQERAPRAYLTGFADSGINLEVGFWIEDPEEGSGALRSDINLEIWKRFKAEGIEIPFP 410 Query: 420 KRDIRLM 426 +R++R++ Sbjct: 411 QREVRML 417