BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0078.p (322 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003777068.1| GGDEF family protein [Herbaspirillum seroped... 421 e-116 ref|YP_004354614.1| Diguanylate cyclase [Pseudomonas brassicacea... 388 e-106 ref|ZP_02886580.1| diguanylate cyclase with GAF sensor [Burkhold... 388 e-106 ref|YP_347908.1| GAF sensor-containing diguanylate cyclase [Pseu... 388 e-106 ref|ZP_04938834.1| Diguanylate cyclase (GGDEF domain) [Burkholde... 387 e-105 >ref|YP_003777068.1| GGDEF family protein [Herbaspirillum seropedicae SmR1] gb|ADJ65160.1| GGDEF family protein [Herbaspirillum seropedicae SmR1] Length = 324 Score = 421 bits (1082), Expect = e-116, Method: Compositional matrix adjust. Identities = 203/320 (63%), Positives = 252/320 (78%), Gaps = 1/320 (0%) Query: 1 MKIADIPVDDQARVADLHGLQILDTPPEERFDRLTRLARRLFSVPMAVVSLVDTKRQWFK 60 M+IA++P ++ AR+ LH L+ILDTP EERFDRLTRLARRLF VPMAVV+++D RQWFK Sbjct: 1 MRIAELPANEAARIDALHSLRILDTPREERFDRLTRLARRLFDVPMAVVTMIDVNRQWFK 60 Query: 61 ARVGV-DACETGRDVSFCAHAILRDDVMVVPDALEDERFRDNPLVTGDPLIRFYAGCPIR 119 + VG+ DA ET R+VSFCAHAIL +++ +VPDA D RF NP VTG+P IRFYAGCPI+ Sbjct: 61 SCVGLGDATETSREVSFCAHAILHEEMTIVPDATLDPRFSGNPQVTGEPFIRFYAGCPIK 120 Query: 120 SPHGHKLGTLCLLDTIPRRFDADDQALLSDLARMVEQEMVAVHLATMDELTKLSNRRGFE 179 S G LGTLCL+DT+PR FDAD++ALL DLA MVEQE+ AV +AT+DELT +SNRRGF+ Sbjct: 121 SESGFTLGTLCLVDTVPRSFDADERALLKDLAAMVEQELTAVRMATIDELTGISNRRGFD 180 Query: 180 SLSQHALSMCKRLGKPASLLYFDLDLFKQINDRFGHAEGDRALVAFSNILAANFRESDVI 239 +L++HAL+ +R+ PA +LYFDLD FKQINDRFGHAEGD ALV F+ +L+++FRESDVI Sbjct: 181 ALARHALANSRRMDWPACMLYFDLDQFKQINDRFGHAEGDAALVDFAALLSSSFRESDVI 240 Query: 240 GRLGGDEFAVFLTNTGAQETATVLGHFEQAIDLHNQISKRGYDLLYSVGAIEYDATGHDT 299 RLGGDEFAVFLTN E+ TVL HF Q++ N + R Y L YSVG ++YDA H Sbjct: 241 ARLGGDEFAVFLTNADLAESDTVLAHFAQSVREFNVLRPRPYALAYSVGVVQYDAARHAD 300 Query: 300 IGDLMSSADALMYQQKKRRR 319 + L SADALMY++KK RR Sbjct: 301 LAALQHSADALMYERKKTRR 320 >ref|YP_004354614.1| Diguanylate cyclase [Pseudomonas brassicacearum subsp. brassicacearum NFM421] gb|AEA69610.1| putative Diguanylate cyclase [Pseudomonas brassicacearum subsp. brassicacearum NFM421] Length = 324 Score = 388 bits (996), Expect = e-106, Method: Compositional matrix adjust. Identities = 187/313 (59%), Positives = 237/313 (75%) Query: 7 PVDDQARVADLHGLQILDTPPEERFDRLTRLARRLFSVPMAVVSLVDTKRQWFKARVGVD 66 P D+ AR+ +LH L++LDT PEERFDRLTRLARRLF VP+A+VSLVD RQWFK+ G+D Sbjct: 11 PDDEAARLKNLHSLKLLDTAPEERFDRLTRLARRLFDVPIALVSLVDANRQWFKSSAGLD 70 Query: 67 ACETGRDVSFCAHAILRDDVMVVPDALEDERFRDNPLVTGDPLIRFYAGCPIRSPHGHKL 126 A ET R++SFC HAIL+D ++ + DA +DERF DNPLVT P IRFYAG P+ G KL Sbjct: 71 ASETPREISFCGHAILQDQILEICDAEQDERFHDNPLVTDKPGIRFYAGHPLGMEDGSKL 130 Query: 127 GTLCLLDTIPRRFDADDQALLSDLARMVEQEMVAVHLATMDELTKLSNRRGFESLSQHAL 186 GTLCLLDT PR+ + +++ LL DLARM EQEMVAV +A+MDELT LSNRRGF +L+QHAL Sbjct: 131 GTLCLLDTKPRKLNDEERELLRDLARMAEQEMVAVQMASMDELTLLSNRRGFRTLAQHAL 190 Query: 187 SMCKRLGKPASLLYFDLDLFKQINDRFGHAEGDRALVAFSNILAANFRESDVIGRLGGDE 246 ++C RL +PA+LL+FDL+ FK INDR+GHAEGD AL F+++L FRESDVIGRLGGDE Sbjct: 191 NVCDRLSRPATLLFFDLNDFKPINDRYGHAEGDNALKTFADVLRIAFRESDVIGRLGGDE 250 Query: 247 FAVFLTNTGAQETATVLGHFEQAIDLHNQISKRGYDLLYSVGAIEYDATGHDTIGDLMSS 306 F LT + E A ++ ++ +D N + +RGYD+ +SVG IEYD H +I L++ Sbjct: 251 FVALLTGSSHVEIAAIMARLQEILDERNAMLQRGYDIRFSVGQIEYDPQRHQSIDMLLAD 310 Query: 307 ADALMYQQKKRRR 319 ADA MY QK+ R Sbjct: 311 ADAAMYAQKQALR 323 >ref|ZP_02886580.1| diguanylate cyclase with GAF sensor [Burkholderia graminis C4D1M] gb|EDT07809.1| diguanylate cyclase with GAF sensor [Burkholderia graminis C4D1M] Length = 326 Score = 388 bits (996), Expect = e-106, Method: Compositional matrix adjust. Identities = 194/316 (61%), Positives = 237/316 (75%) Query: 1 MKIADIPVDDQARVADLHGLQILDTPPEERFDRLTRLARRLFSVPMAVVSLVDTKRQWFK 60 M IP ++ R+ L L ILDTPPEERFDRLTRLA+RLF VP+A+VSLVD RQWFK Sbjct: 1 MHTPPIPSNESTRLDTLRALHILDTPPEERFDRLTRLAKRLFGVPIALVSLVDANRQWFK 60 Query: 61 ARVGVDACETGRDVSFCAHAILRDDVMVVPDALEDERFRDNPLVTGDPLIRFYAGCPIRS 120 + VG+ A ET RDVSFCAHAIL DD++ VPD L DERF DNPLVT P IRFYAGCP+ Sbjct: 61 SCVGLTASETSRDVSFCAHAILDDDILAVPDTLADERFHDNPLVTEAPNIRFYAGCPLTV 120 Query: 121 PHGHKLGTLCLLDTIPRRFDADDQALLSDLARMVEQEMVAVHLATMDELTKLSNRRGFES 180 P+G +LGTLCL+D PR D +++ LL DLARM EQE+ AV LAT+D+LT LSNRRGF + Sbjct: 121 PNGSRLGTLCLIDVKPRELDDEERELLRDLARMAEQELAAVQLATLDDLTLLSNRRGFSA 180 Query: 181 LSQHALSMCKRLGKPASLLYFDLDLFKQINDRFGHAEGDRALVAFSNILAANFRESDVIG 240 L+QHAL++CKRL +PASLL+FDL+ FKQIND FGHAEGD AL F+++L RESDVIG Sbjct: 181 LAQHALNVCKRLDRPASLLFFDLNDFKQINDTFGHAEGDHALKTFADVLRGTLRESDVIG 240 Query: 241 RLGGDEFAVFLTNTGAQETATVLGHFEQAIDLHNQISKRGYDLLYSVGAIEYDATGHDTI 300 RLGGDEF V L++T A T V ++D N ++RGY + +SVG IEYDA H +I Sbjct: 241 RLGGDEFVVLLSDTDAAATQEVTERLTHSLDERNAQAQRGYSIRFSVGQIEYDAARHGSI 300 Query: 301 GDLMSSADALMYQQKK 316 +L+S+ADA MY K+ Sbjct: 301 AELLSAADAAMYSHKQ 316 >ref|YP_347908.1| GAF sensor-containing diguanylate cyclase [Pseudomonas fluorescens Pf0-1] gb|ABA73919.1| putative GGDEF/GAF domain sensory box protein [Pseudomonas fluorescens Pf0-1] Length = 321 Score = 388 bits (996), Expect = e-106, Method: Compositional matrix adjust. Identities = 183/316 (57%), Positives = 239/316 (75%) Query: 1 MKIADIPVDDQARVADLHGLQILDTPPEERFDRLTRLARRLFSVPMAVVSLVDTKRQWFK 60 M + P ++ R+ L L++LDT PEERFDRLTRLA+RLF+VP+A+V+LVD +RQWFK Sbjct: 1 MLVPGKPANEAVRIQALQDLKLLDTAPEERFDRLTRLAKRLFNVPIALVTLVDKERQWFK 60 Query: 61 ARVGVDACETGRDVSFCAHAILRDDVMVVPDALEDERFRDNPLVTGDPLIRFYAGCPIRS 120 + VG+D ET R VSFCAHAILRD++M+VPDA EDERF DNPLVTG+P IRFYAG P+ Sbjct: 61 SCVGLDTTETPRSVSFCAHAILRDELMLVPDAREDERFHDNPLVTGEPNIRFYAGYPLTV 120 Query: 121 PHGHKLGTLCLLDTIPRRFDADDQALLSDLARMVEQEMVAVHLATMDELTKLSNRRGFES 180 P G+K+GTLCL+DT PR D +++ALL DLA M EQE++AV +A+MDELT LSNRRGF+ Sbjct: 121 PSGNKMGTLCLIDTKPRDLDEEERALLRDLAGMAEQELMAVQMASMDELTLLSNRRGFKM 180 Query: 181 LSQHALSMCKRLGKPASLLYFDLDLFKQINDRFGHAEGDRALVAFSNILAANFRESDVIG 240 L+QHAL C RL KPA+LL+FDL+ FKQIND +GHAEGD AL F+++L FRESDV+G Sbjct: 181 LAQHALDACARLEKPATLLFFDLNDFKQINDLYGHAEGDSALKTFADVLRIAFRESDVVG 240 Query: 241 RLGGDEFAVFLTNTGAQETATVLGHFEQAIDLHNQISKRGYDLLYSVGAIEYDATGHDTI 300 RLGGDEF LT + ET ++ ++ ++ N RGY + +SVG IEY+ H+++ Sbjct: 241 RLGGDEFVALLTGSSHVETTAIMARLKEILEERNATLHRGYAIRFSVGQIEYNPQRHESV 300 Query: 301 GDLMSSADALMYQQKK 316 L++ ADA MY+ K+ Sbjct: 301 DKLLADADAAMYEHKQ 316 >ref|ZP_04938834.1| Diguanylate cyclase (GGDEF domain) [Burkholderia cenocepacia PC184] gb|EAY62005.1| Diguanylate cyclase (GGDEF domain) [Burkholderia cenocepacia PC184] Length = 335 Score = 387 bits (994), Expect = e-105, Method: Compositional matrix adjust. Identities = 190/321 (59%), Positives = 233/321 (72%) Query: 1 MKIADIPVDDQARVADLHGLQILDTPPEERFDRLTRLARRLFSVPMAVVSLVDTKRQWFK 60 M+IA P ++ AR+ LH L ILDTPPEERFDRLTRLARRLF VP+A+VSLVD RQWFK Sbjct: 13 MQIAPKPANEAARLDTLHSLSILDTPPEERFDRLTRLARRLFDVPIALVSLVDENRQWFK 72 Query: 61 ARVGVDACETGRDVSFCAHAILRDDVMVVPDALEDERFRDNPLVTGDPLIRFYAGCPIRS 120 + G+DA +T RDVSFCAHA+L D MV+ DAL D+RF DNPLVTG P IRFYAG P+ + Sbjct: 73 SHAGLDATQTPRDVSFCAHALLSGDTMVIQDALNDDRFHDNPLVTGTPGIRFYAGRPLTA 132 Query: 121 PHGHKLGTLCLLDTIPRRFDADDQALLSDLARMVEQEMVAVHLATMDELTKLSNRRGFES 180 P+G +GTLCL+DT PR +AD+ ALL DLA M E+E+ A+H AT DELT+L+NRRGFE Sbjct: 133 PNGAPVGTLCLIDTRPRSLEADELALLGDLAHMTEREIAALHFATTDELTQLTNRRGFEI 192 Query: 181 LSQHALSMCKRLGKPASLLYFDLDLFKQINDRFGHAEGDRALVAFSNILAANFRESDVIG 240 L++H LS+C R+G+ A LL+FDL+ FK INDRFGHAEGDRAL AF L R+ DVI Sbjct: 193 LARHVLSLCDRVGRHAMLLFFDLNDFKAINDRFGHAEGDRALKAFGETLTGALRDGDVIA 252 Query: 241 RLGGDEFAVFLTNTGAQETATVLGHFEQAIDLHNQISKRGYDLLYSVGAIEYDATGHDTI 300 RLGGDEF V L+ E A + QA+ N RGY + +SVG + YD H T+ Sbjct: 253 RLGGDEFVVLLSAADPAEVAEPVERVTQALAQRNAADARGYAIRFSVGHVAYDPARHRTV 312 Query: 301 GDLMSSADALMYQQKKRRREA 321 DL++SAD LMY+ K+R R A Sbjct: 313 ADLLASADRLMYEHKQRGRTA 333