BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0024.p (268 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_08274308.1| Vitamin B12 ABC transporter, B12-binding comp... 182 4e-44 ref|YP_004754132.1| Vitamin B12 ABC transporter B12-binding comp... 167 2e-39 ref|YP_727409.1| cobalamin-binding protein (BtuF) [Ralstonia eut... 147 2e-33 ref|YP_004227275.1| periplasmic-binding protein [Burkholderia sp... 145 8e-33 ref|YP_003604327.1| periplasmic binding protein [Burkholderia sp... 145 8e-33 >ref|ZP_08274308.1| Vitamin B12 ABC transporter, B12-binding component BtuF [Oxalobacteraceae bacterium IMCC9480] gb|EGF32218.1| Vitamin B12 ABC transporter, B12-binding component BtuF [Oxalobacteraceae bacterium IMCC9480] Length = 292 Score = 182 bits (462), Expect = 4e-44, Method: Compositional matrix adjust. Identities = 119/290 (41%), Positives = 163/290 (56%), Gaps = 41/290 (14%) Query: 15 VLALLCAGNAA----ASITVTDDFGNDIRLDKPARRIVSLSPHTTELLYAAGAGPYVIGV 70 ++ALL A + A A ++ TDD G+ IRLD PARRI+SL+PH TELL+AAGAG ++GV Sbjct: 5 LIALLLATSGALAEAAPVSATDDAGHPIRLDAPARRIISLAPHVTELLFAAGAGARLVGV 64 Query: 71 SNYSNYPPAALQAVSVGRISAVDIEKILTLKPDLILAWRSGNSAXQSRRRFPRALASAKP 130 S+YS++PPAA SVG +A+DIE+I+ LKPDL++AW SGNSA Q R RAL Sbjct: 65 SDYSDFPPAAKAIASVGGAAALDIERIVMLKPDLVVAWGSGNSASQLARL--RALGITVF 122 Query: 131 QLSRPQAGQRVLSDLGTPADDAQRQAHG----VDGAAHANT--------DAAPLLHTVGR 178 S P+ + S L A A +A G +D A T + P+ + + R Sbjct: 123 D-SEPRDFAAIASSLERLATLAGSEATGRAAAIDFRARLQTLTTTYQQRASVPVFYQIWR 181 Query: 179 RRLRTLFHRAAKP----------------------GSQSVIAANPEAILAPDDAKDHPLD 216 L TL ++ G+++V+ ANPE IL D + L Sbjct: 182 APLMTLNGQSMVSTAIRLCGGNNIFAQLPQLAPVIGTEAVLQANPEVILTGGDDEAAALA 241 Query: 217 EWRQFGKMKAVAAGNLFTVNADWLNRPGPRVLDATEEVCKILDTVRSRRP 266 +WR+F + AV NLF +NADWL R GPR+L+ TE +C+ LDT R +RP Sbjct: 242 DWRRFPSLLAVQRNNLFVINADWLARSGPRLLEGTELLCRQLDTARRKRP 291 >ref|YP_004754132.1| Vitamin B12 ABC transporter B12-binding component BtuF [Collimonas fungivorans Ter331] gb|AEK63309.1| Vitamin B12 ABC transporter, B12-binding component BtuF [Collimonas fungivorans Ter331] Length = 304 Score = 167 bits (423), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 108/268 (40%), Positives = 145/268 (54%), Gaps = 38/268 (14%) Query: 32 DDFGNDIRLDKPARRIVSLSPHTTELLYAAGAGPYVIGVSNYSNYPPAALQAVSVGRISA 91 DD + + L PA RIVSL+PH TELL+AAGAG YV+GVS+YS+YPP A Q S+G SA Sbjct: 40 DDTQHAVVLHHPAHRIVSLAPHATELLFAAGAGAYVVGVSDYSDYPPEAKQITSMGSSSA 99 Query: 92 VDIEKILTLKPDLILAWRSGNSAXQSRR----------RFPRALASAKPQLSRPQ--AGQ 139 +DIE+I+ LKPDL++AW SGNSA Q R PR L + L R AG Sbjct: 100 LDIERIVALKPDLVVAWSSGNSASQIARLRAIGIPVFESEPRQLEAIAASLLRLSQLAGT 159 Query: 140 RVLSDLGTPADDAQRQAHGVDGAAHANTDAAPLLHTVGRRRLRTL--------------- 184 D G A DA + A + + + + ++ L TL Sbjct: 160 E---DSGAAAADAFKTRLAQLDATYRQRPPVRVFYQIWKKPLMTLNDSHMVSSVIALCGG 216 Query: 185 ---FHR----AAKPGSQSVIAANPEAILAPDDAKDHPLDEWRQFGKMKAVAAGNLFTVNA 237 F + A +++V+ A+PE I A D P WR+F K+ A+AA NLFT+ Sbjct: 217 ENVFGKLPQLAPTVSTEAVLQADPEVIFAADGGSS-PSAGWRRFPKLTAIAADNLFTLTP 275 Query: 238 DWLNRPGPRVLDATEEVCKILDTVRSRR 265 +W++RPGPR+LD E+C+ LD RSRR Sbjct: 276 NWMSRPGPRILDGAAELCQKLDLARSRR 303 >ref|YP_727409.1| cobalamin-binding protein (BtuF) [Ralstonia eutropha H16] emb|CAJ94041.1| Cobalamin-binding protein (BtuF) [Ralstonia eutropha H16] Length = 301 Score = 147 bits (370), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 94/277 (33%), Positives = 142/277 (51%), Gaps = 37/277 (13%) Query: 27 SITVTDDFGNDIRLDKPARRIVSLSPHTTELLYAAGAGPYVIGVSNYSNYPPAALQAVSV 86 +++ DD G + L +PARR++SL+PH TE+++AAG G ++G +YS+YPP A + V Sbjct: 23 TVSAVDDAGQTVTLPQPARRVISLAPHVTEMIFAAGGGDRIVGTVSYSDYPPQAREIARV 82 Query: 87 GRISAVDIEKILTLKPDLILAWRSGNSAXQSRRR----FPRALASAKPQLSRPQAGQRVL 142 G A+D+E+I LKPDLI+ WR GN+ Q+ R P + + + P+ +++ Sbjct: 83 GDNKALDLERIAALKPDLIVVWRHGNAQRQTDRLRALGIPLFYSEPRRLAAIPENMEKLG 142 Query: 143 SDLGTPAD-----DAQRQAHGVDGAAHANTDAAPLLHTVGRRRLRTL------------- 184 + LGT A D RQ +A + + V ++ L TL Sbjct: 143 TLLGTEATARRAADGFRQQVETLRKTYAARPPVTVFYQVWQQPLMTLNGQHLVSDMLALC 202 Query: 185 ----FHRAAKP-----GSQSVIAANPEAILAPDDA---KDHPL---DEWRQFGKMKAVAA 229 A P ++VIA NPEA+L D PL + WR++ ++ AVA Sbjct: 203 GGRNLFAAETPLVPTVSVEAVIAGNPEAMLTAGMGATRSDQPLPDFEMWRRWKQVTAVAR 262 Query: 230 GNLFTVNADWLNRPGPRVLDATEEVCKILDTVRSRRP 266 NLF ++ D +NR GPRV E +CK L+T R RRP Sbjct: 263 NNLFVIDGDLVNRAGPRVAQGAELICKDLETARKRRP 299 >ref|YP_004227275.1| periplasmic-binding protein [Burkholderia sp. CCGE1001] gb|ADX54215.1| periplasmic binding protein [Burkholderia sp. CCGE1001] Length = 309 Score = 145 bits (365), Expect = 8e-33, Method: Compositional matrix adjust. Identities = 103/281 (36%), Positives = 149/281 (53%), Gaps = 37/281 (13%) Query: 24 AAASITVTDDFGNDIRLDKPARRIVSLSPHTTELLYAAGAGPYVIGVSNYSNYPPAALQA 83 A A ITVTDD G + L A+R++SL+PH TELLYAAG ++G +YS+YPP A Q Sbjct: 26 ARAGITVTDDTGAAVTLPAAAQRVISLAPHVTELLYAAGGASKMVGAVSYSDYPPEAKQL 85 Query: 84 VSVGRISAVDIEKILTLKPDLILAWRSGNSAXQSR--RRFPRALASAKPQ------LSRP 135 VG ++D+E+I+ LKPDLI+ WR GN+ Q R L ++P +S Sbjct: 86 PRVGDNKSLDLERIVALKPDLIVVWRHGNAQRQLERVRELHIPLFFSEPHRLDDIAVSLT 145 Query: 136 QAGQRV-LSDLGTPADDAQRQAHGVDGAAHANTDAAPLLHTVGRRRLRTL---------- 184 + GQ + S PA A R+ A +A A + + V + L TL Sbjct: 146 KLGQLLGTSSTADPAAAAYRRDIANLRAQYAKRPAVSVFYQVWDQPLMTLNGEHMVSDVI 205 Query: 185 -------FHRAAKP-----GSQSVIAANPEAIL--APDDAK-DHPLDE---WRQFGKMKA 226 A +P +++V+AANPEAI+ AP K D PL + WR + ++ A Sbjct: 206 ELCGGRNVFAALQPLVPTVSTEAVLAANPEAIVTAAPGATKPDTPLPQLGAWRAWPRLLA 265 Query: 227 VAAGNLFTVNADWLNRPGPRVLDATEEVCKILDTVRSRRPP 267 VA NLF ++ D +NRP PR++ +++C+ L+ RSRR P Sbjct: 266 VADNNLFAIDGDLINRPSPRIVQGAKQMCEDLELARSRRKP 306 >ref|YP_003604327.1| periplasmic binding protein [Burkholderia sp. CCGE1002] gb|ADG14816.1| periplasmic binding protein [Burkholderia sp. CCGE1002] Length = 316 Score = 145 bits (365), Expect = 8e-33, Method: Compositional matrix adjust. Identities = 100/283 (35%), Positives = 145/283 (51%), Gaps = 37/283 (13%) Query: 19 LCAGNAAASITVTDDFGNDIRLDKPARRIVSLSPHTTELLYAAGAGPYVIGVSNYSNYPP 78 + A +A ASITVTDD G + L PA+R++SL+PH TELLYAAG GP ++G +YS+YPP Sbjct: 28 VTALSAHASITVTDDSGATVTLAAPAQRVISLAPHVTELLYAAGGGPKMVGAVSYSDYPP 87 Query: 79 AALQAVSVGRISAVDIEKILTLKPDLILAWRSGNSAXQSR--RRFPRALASAKPQ----- 131 A Q VG A+D+E+I+ LKPDLI+ WR GN+ Q R L ++P+ Sbjct: 88 EAKQLPRVGDNKALDLERIVALKPDLIVVWRHGNAQHQLERLRELHVPLFFSEPRRLDDI 147 Query: 132 -LSRPQAGQRV-LSDLGTPADDAQRQAHGVDGAAHANTDAAPLLHTVGRRRLRTLFHR-- 187 +S + G+ + S A A R+ A +AN + + V R L TL Sbjct: 148 AVSLTKLGRLLGTSTSADAAAAAYREDIARLRARYANRPVVSVFYQVWDRPLMTLNDEHM 207 Query: 188 --------------------AAKPGSQSVIAANPEAILAPDDAKDHP------LDEWRQF 221 +++V+AANP+AI+ P LD WR + Sbjct: 208 ISDVITLCGGRNVFADLQPLVPTVSTEAVLAANPQAIVTAASGATQPDTTLPQLDTWRAW 267 Query: 222 GKMKAVAAGNLFTVNADWLNRPGPRVLDATEEVCKILDTVRSR 264 ++ AVA NLF ++ D +NRP PR+ ++C+ L+ RSR Sbjct: 268 PRLAAVANNNLFAIDGDLINRPAPRIAQGARQLCEDLELARSR 310