BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0026.0101.p (365 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003774868.1| L-asparaginase/archaeal Glu-tRNAGln amidotra... 495 e-138 ref|YP_296021.1| L-asparaginase, type II [Ralstonia eutropha JMP... 419 e-115 ref|YP_004685710.1| glutaminase-asparaginase AnsB [Cupriavidus n... 411 e-112 ref|YP_726379.1| glutaminase-asparaginase (amidohydrolase) [Rals... 409 e-112 ref|YP_583735.1| periplasmic L-asparaginase II [Cupriavidus meta... 406 e-111 >ref|YP_003774868.1| L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D protein [Herbaspirillum seropedicae SmR1] emb|CAM32630.1| L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D protein [Herbaspirillum seropedicae] gb|ADJ62960.1| L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D protein [Herbaspirillum seropedicae SmR1] Length = 356 Score = 495 bits (1275), Expect = e-138, Method: Compositional matrix adjust. Identities = 251/356 (70%), Positives = 294/356 (82%), Gaps = 10/356 (2%) Query: 11 GSLLVFLASFCLCLQAQEK-PHIVVLATGGTIAGANISSIRSPAFQAAVVPVDRMIAAVP 69 G++++ + QAQ+K P++VV+ TGGTIAGA SS+ + A+Q+AVVPVD++IAAVP Sbjct: 10 GAVMILSLACAFSAQAQDKKPNVVVIGTGGTIAGAGASSVNTAAYQSAVVPVDKIIAAVP 69 Query: 70 EIQKIAHVTGEQIFQVPSENFTDERLIKLGKRVSELLKKSNVDGIVITDTMEETAYFLNF 129 EI KIA+V GEQIFQ+ SE+F DERLIKLGKRVSELLK+++VDGIVIT Sbjct: 70 EISKIANVRGEQIFQIGSESFNDERLIKLGKRVSELLKQNDVDGIVITHGT--------- 120 Query: 130 DTMEETAYFLNLTLKSTKPVVLVGAMRASTQLGSDGSANFHDAIAVAASKEAIGKGTLVS 189 DT+EETAYFLNL LKS KPVV+VG+MR T LG+DG+ N +DA+ VA SKEA GKGTLV Sbjct: 121 DTIEETAYFLNLVLKSDKPVVVVGSMRPGTALGADGALNLYDAVLVAGSKEAAGKGTLVV 180 Query: 190 MNDEIYSARDVTMGNTLKLETLRSPYGPLGLIIEGKVMFYRQPTRPHTVASEFDIDRLST 249 MNDEI+S RDVT NT K+ET RSPYGPLG ++E K+ FYR P RPHT +EFDID++S+ Sbjct: 181 MNDEIHSGRDVTKSNTFKVETFRSPYGPLGYVVENKLSFYRLPARPHTTQTEFDIDKISS 240 Query: 250 LPRVDIVYNYGNVSPVAYEALVNAGAKAIIHNGTGNGSVAEQIVPVLQEVRSKGLQVVRA 309 LPRVDIVYNYGNVS AY+A V AGAKAIIH+GTGNGSVAEQIVPVLQEVRSKG+QV+RA Sbjct: 241 LPRVDIVYNYGNVSRTAYDAFVAAGAKAIIHDGTGNGSVAEQIVPVLQEVRSKGVQVIRA 300 Query: 310 TRTGSGVVIRNGEQNDDKFDWVVTDDQNPQKARILTALALTKTNDSKELQKILWKY 365 +RTGSGVVIRNGEQ DDKFDWVVTDDQN QKARIL LALTKTND KELQKI+WKY Sbjct: 301 SRTGSGVVIRNGEQPDDKFDWVVTDDQNAQKARILAELALTKTNDPKELQKIMWKY 356 >ref|YP_296021.1| L-asparaginase, type II [Ralstonia eutropha JMP134] gb|AAZ61177.1| L-asparaginase, type II [Ralstonia eutropha JMP134] Length = 374 Score = 419 bits (1077), Expect = e-115, Method: Compositional matrix adjust. Identities = 199/319 (62%), Positives = 253/319 (79%), Gaps = 9/319 (2%) Query: 47 SSIRSPAFQAAVVPVDRMIAAVPEIQKIAHVTGEQIFQVPSENFTDERLIKLGKRVSELL 106 S+ + A+Q+AVVPVD++IA+VPEI K+A+V GEQIFQ+ SE+F +ERL+KLGKRVSELL Sbjct: 65 SATNTAAYQSAVVPVDKIIASVPEISKVANVKGEQIFQIGSESFNNERLLKLGKRVSELL 124 Query: 107 KKSNVDGIVITDTMEETAYFLNFDTMEETAYFLNLTLKSTKPVVLVGAMRASTQLGSDGS 166 K+ +VDGIVIT DT+EETAYFLNLTLKS KPVV+VG+MR T LG+DG+ Sbjct: 125 KQPDVDGIVITH---------GTDTIEETAYFLNLTLKSDKPVVVVGSMRPGTALGADGA 175 Query: 167 ANFHDAIAVAASKEAIGKGTLVSMNDEIYSARDVTMGNTLKLETLRSPYGPLGLIIEGKV 226 N +DA+ VA++ + GKGTL +NDEI++ RDVT NT K ET RSP+GPLG ++EG+ Sbjct: 176 LNLYDAVLVASNPASRGKGTLAVLNDEIHTGRDVTKSNTFKTETFRSPFGPLGYVVEGRT 235 Query: 227 MFYRQPTRPHTVASEFDIDRLSTLPRVDIVYNYGNVSPVAYEALVNAGAKAIIHNGTGNG 286 +FYR P RPHT +EFDID++ LP V +VY YGNV+P + +A V GAKAII+ TGNG Sbjct: 236 LFYRLPARPHTTQTEFDIDKIDKLPEVAVVYAYGNVNPGSVDAAVKNGAKAIIYAATGNG 295 Query: 287 SVAEQIVPVLQEVRSKGLQVVRATRTGSGVVIRNGEQNDDKFDWVVTDDQNPQKARILTA 346 SV + +V L+ R+KG+Q+VRA+RTGSGVVIRN EQ DDK+DW+VTDDQ PQKARIL A Sbjct: 296 SVGDYMVEPLKAARAKGVQIVRASRTGSGVVIRNAEQPDDKYDWIVTDDQLPQKARILMA 355 Query: 347 LALTKTNDSKELQKILWKY 365 LALTKTNDSK+LQ+++WKY Sbjct: 356 LALTKTNDSKQLQQVMWKY 374 >ref|YP_004685710.1| glutaminase-asparaginase AnsB [Cupriavidus necator N-1] gb|AEI77229.1| glutaminase-asparaginase AnsB [Cupriavidus necator N-1] Length = 374 Score = 411 bits (1056), Expect = e-112, Method: Compositional matrix adjust. Identities = 193/319 (60%), Positives = 250/319 (78%), Gaps = 9/319 (2%) Query: 47 SSIRSPAFQAAVVPVDRMIAAVPEIQKIAHVTGEQIFQVPSENFTDERLIKLGKRVSELL 106 S+ + A+Q+AVVPVD++IA+VPEI K+A+V GEQIFQ+ SE+F +ERL+KL KRVSELL Sbjct: 65 SATNTAAYQSAVVPVDKIIASVPEISKVANVKGEQIFQIGSESFNNERLLKLAKRVSELL 124 Query: 107 KKSNVDGIVITDTMEETAYFLNFDTMEETAYFLNLTLKSTKPVVLVGAMRASTQLGSDGS 166 K+ +VDGIVIT DT+EETAYFLNLTLKS KPVV+VG+MR T LG+DG+ Sbjct: 125 KQPDVDGIVITH---------GTDTIEETAYFLNLTLKSDKPVVVVGSMRPGTALGADGA 175 Query: 167 ANFHDAIAVAASKEAIGKGTLVSMNDEIYSARDVTMGNTLKLETLRSPYGPLGLIIEGKV 226 N +DA+ VA++ + GKGTL +NDEI++ RDVT NT K ET RSP+GPLG ++EG+ Sbjct: 176 LNLYDAVLVASNPASKGKGTLAVLNDEIHTGRDVTKSNTFKTETFRSPFGPLGYVVEGRT 235 Query: 227 MFYRQPTRPHTVASEFDIDRLSTLPRVDIVYNYGNVSPVAYEALVNAGAKAIIHNGTGNG 286 +FYR P RPHT +++DID++ LP V ++Y YGN +P A +A V +GAKAII+ TGNG Sbjct: 236 LFYRLPARPHTQQTQWDIDKIDKLPEVAVLYAYGNANPAAVDAAVKSGAKAIIYAATGNG 295 Query: 287 SVAEQIVPVLQEVRSKGLQVVRATRTGSGVVIRNGEQNDDKFDWVVTDDQNPQKARILTA 346 SV + +V L+ R+KG+Q+VRA+RTGSGVV+RN EQ DDK+DW+VTDDQ PQKARIL A Sbjct: 296 SVGDYMVESLKAARAKGVQIVRASRTGSGVVVRNAEQPDDKYDWIVTDDQLPQKARILMA 355 Query: 347 LALTKTNDSKELQKILWKY 365 LALT+TNDSK LQ++ WKY Sbjct: 356 LALTQTNDSKALQQVFWKY 374 >ref|YP_726379.1| glutaminase-asparaginase (amidohydrolase) [Ralstonia eutropha H16] emb|CAJ93011.1| Glutaminase-asparaginase (amidohydrolase) [Ralstonia eutropha H16] Length = 374 Score = 409 bits (1050), Expect = e-112, Method: Compositional matrix adjust. Identities = 192/319 (60%), Positives = 250/319 (78%), Gaps = 9/319 (2%) Query: 47 SSIRSPAFQAAVVPVDRMIAAVPEIQKIAHVTGEQIFQVPSENFTDERLIKLGKRVSELL 106 S + A+Q+AVVPVD++IA+VPEI K+A+V GEQIFQ+ SE+F +ERL+KL KRVSELL Sbjct: 65 SVTNTAAYQSAVVPVDKIIASVPEISKVANVKGEQIFQIGSESFNNERLLKLAKRVSELL 124 Query: 107 KKSNVDGIVITDTMEETAYFLNFDTMEETAYFLNLTLKSTKPVVLVGAMRASTQLGSDGS 166 K+ +VDGIVIT DT+EETAYFLNLTLKS KPVV+VG+MR T LG+DG+ Sbjct: 125 KQPDVDGIVITH---------GTDTIEETAYFLNLTLKSDKPVVVVGSMRPGTALGADGA 175 Query: 167 ANFHDAIAVAASKEAIGKGTLVSMNDEIYSARDVTMGNTLKLETLRSPYGPLGLIIEGKV 226 N +DA+ VA++ + GKGTL +NDEI++ RDVT NT K ET RSP+GPLG ++EG+ Sbjct: 176 LNLYDAVLVASNPASKGKGTLAVLNDEIHTGRDVTKSNTFKTETFRSPFGPLGYVVEGRT 235 Query: 227 MFYRQPTRPHTVASEFDIDRLSTLPRVDIVYNYGNVSPVAYEALVNAGAKAIIHNGTGNG 286 +FYR P RPHT+ +++DID++ LP V ++Y YGN +P A +A V +GAKAII+ TGNG Sbjct: 236 LFYRLPARPHTLQTQWDIDKIDKLPEVAVLYAYGNANPAAVDAAVKSGAKAIIYAATGNG 295 Query: 287 SVAEQIVPVLQEVRSKGLQVVRATRTGSGVVIRNGEQNDDKFDWVVTDDQNPQKARILTA 346 SV + +V L+ R+KG+Q+VRA+RTGSGVV+RN EQ DDK+DW+VTDDQ PQKARIL A Sbjct: 296 SVGDYMVESLKAARAKGVQIVRASRTGSGVVVRNAEQPDDKYDWIVTDDQLPQKARILMA 355 Query: 347 LALTKTNDSKELQKILWKY 365 LALT+TND+K LQ++ WKY Sbjct: 356 LALTQTNDAKALQQVFWKY 374 >ref|YP_583735.1| periplasmic L-asparaginase II [Cupriavidus metallidurans CH34] gb|ABF08466.1| periplasmic L-asparaginase II [Cupriavidus metallidurans CH34] Length = 375 Score = 406 bits (1044), Expect = e-111, Method: Compositional matrix adjust. Identities = 195/312 (62%), Positives = 246/312 (78%), Gaps = 9/312 (2%) Query: 54 FQAAVVPVDRMIAAVPEIQKIAHVTGEQIFQVPSENFTDERLIKLGKRVSELLKKSNVDG 113 +Q+AVVPVD++IA+VPEI KIA+V GEQ+FQ+ SE+F +ERLIKL KRVSELLK+ +VDG Sbjct: 73 YQSAVVPVDKIIASVPEISKIANVKGEQVFQIGSESFNNERLIKLAKRVSELLKQPDVDG 132 Query: 114 IVITDTMEETAYFLNFDTMEETAYFLNLTLKSTKPVVLVGAMRASTQLGSDGSANFHDAI 173 IVIT DT+EETAYFLNLTLKS KPVV+VG+MR T LG+DG+ N +DA+ Sbjct: 133 IVITHGT---------DTIEETAYFLNLTLKSDKPVVVVGSMRPGTALGADGALNLYDAV 183 Query: 174 AVAASKEAIGKGTLVSMNDEIYSARDVTMGNTLKLETLRSPYGPLGLIIEGKVMFYRQPT 233 VA++ + GKGTLV +NDEI + RDVT NT K+ET RSP+GPLG ++EG+ +++R P Sbjct: 184 LVASNPASKGKGTLVVLNDEIQTGRDVTKTNTFKVETFRSPFGPLGYVVEGRTLYFRLPA 243 Query: 234 RPHTVASEFDIDRLSTLPRVDIVYNYGNVSPVAYEALVNAGAKAIIHNGTGNGSVAEQIV 293 RPHT +EFDID++ LP V +VY YGN++P A +A V +GAKAII + TGNGSV + +V Sbjct: 244 RPHTTQTEFDIDKIDKLPEVAVVYAYGNMNPAAIDAAVKSGAKAIIFDATGNGSVGDYMV 303 Query: 294 PVLQEVRSKGLQVVRATRTGSGVVIRNGEQNDDKFDWVVTDDQNPQKARILTALALTKTN 353 L+ R+KG+ +VRA+RTGSGVVIRNGEQ DDK+DW+VTDDQ QKARIL ALALTKTN Sbjct: 304 EPLKAARAKGVFIVRASRTGSGVVIRNGEQPDDKYDWIVTDDQIAQKARILMALALTKTN 363 Query: 354 DSKELQKILWKY 365 D K LQ+I WKY Sbjct: 364 DPKALQEIFWKY 375