BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0026.0050.p (274 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003278099.1| long-chain acyl-CoA synthetase [Comamonas te... 182 3e-44 ref|ZP_07046963.1| long-chain acyl-CoA synthetase [Comamonas tes... 182 5e-44 ref|ZP_03543542.1| AMP-dependent synthetase and ligase [Comamona... 182 6e-44 ref|ZP_08405135.1| AMP-dependent synthetase/ligase [Hylemonella ... 180 2e-43 ref|YP_002553002.1| AMP-dependent synthetase and ligase [Acidovo... 180 2e-43 >ref|YP_003278099.1| long-chain acyl-CoA synthetase [Comamonas testosteroni CNB-2] gb|ACY32803.1| long-chain acyl-CoA synthetase [Comamonas testosteroni CNB-2] Length = 503 Score = 182 bits (463), Expect = 3e-44, Method: Compositional matrix adjust. Identities = 89/154 (57%), Positives = 115/154 (74%), Gaps = 5/154 (3%) Query: 113 GLIYVRSPMVFTDYVTLRDSDEQPALLRDGDWLSVRDVGHLDKQGRLHLLGRQQRMLLAQ 172 GLIY+RSPM+F DYV D+ + A+LRDGDWLSVRD+GH+D++G L L GRQ RML+ Q Sbjct: 327 GLIYIRSPMLFMDYVG--DAHDATAVLRDGDWLSVRDMGHIDERGMLCLAGRQSRMLVTQ 384 Query: 173 GKNLFPEEVESVLTAHPAVAAASVQGMADAVRGVQIAALLK---VTDPVSPEALVAWCRE 229 GKNLFPEEVE++L +HPA+A S+ G ADA+RG+Q+ A+L+ T+ S L W RE Sbjct: 385 GKNLFPEEVENLLASHPAIAQVSLHGQADALRGLQVHAVLQWRPQTEAPSALELNQWLRE 444 Query: 230 RLEPYKIPRRFYTCNDWPLTPSGKTDHKQLAQRL 263 R E +K+PR+++ C WP T SGKTDH QLAQ L Sbjct: 445 RTEAFKVPRQWWVCEHWPHTASGKTDHGQLAQAL 478 Score = 87.8 bits (216), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 39/65 (60%), Positives = 48/65 (73%) Query: 36 SSPFYIGFTSGSSGLPKGFRRSHGSWTSSFDICLESFGAGAAATLLVPGRLSHSTFLFGA 95 ++ FY GFTSGS+GLPKGF+R H SWT SF + L+ FG A L PGR+SHS FLFGA Sbjct: 123 TAAFYTGFTSGSTGLPKGFKRHHLSWTESFRVGLQDFGPVVAQRTLAPGRISHSLFLFGA 182 Query: 96 LLGLW 100 + G+W Sbjct: 183 MQGIW 187 >ref|ZP_07046963.1| long-chain acyl-CoA synthetase [Comamonas testosteroni S44] gb|EFI59294.1| long-chain acyl-CoA synthetase [Comamonas testosteroni S44] Length = 503 Score = 182 bits (462), Expect = 5e-44, Method: Compositional matrix adjust. Identities = 88/154 (57%), Positives = 115/154 (74%), Gaps = 5/154 (3%) Query: 113 GLIYVRSPMVFTDYVTLRDSDEQPALLRDGDWLSVRDVGHLDKQGRLHLLGRQQRMLLAQ 172 GLIY+RSPM+F DYV D+ + A+LRDGDWLSVRD+GH+D++G L L GRQ RM++ Q Sbjct: 327 GLIYIRSPMLFMDYVG--DAHDATAVLRDGDWLSVRDMGHIDERGMLCLAGRQSRMIVTQ 384 Query: 173 GKNLFPEEVESVLTAHPAVAAASVQGMADAVRGVQIAALLK---VTDPVSPEALVAWCRE 229 GKNLFPEEVE++L +HPA+A S+ G ADA+RG+Q+ A+L+ T+ S L W RE Sbjct: 385 GKNLFPEEVENLLASHPAIAQVSLHGQADALRGLQVHAVLQWRPQTEAPSALELNQWLRE 444 Query: 230 RLEPYKIPRRFYTCNDWPLTPSGKTDHKQLAQRL 263 R E +K+PR+++ C WP T SGKTDH QLAQ L Sbjct: 445 RTEAFKVPRQWWVCEHWPHTASGKTDHGQLAQAL 478 Score = 87.8 bits (216), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 39/65 (60%), Positives = 48/65 (73%) Query: 36 SSPFYIGFTSGSSGLPKGFRRSHGSWTSSFDICLESFGAGAAATLLVPGRLSHSTFLFGA 95 ++ FY GFTSGS+GLPKGF+R H SWT SF + L+ FG A L PGR+SHS FLFGA Sbjct: 123 TAAFYTGFTSGSTGLPKGFKRHHLSWTESFRVGLQDFGPVVAQRTLAPGRISHSLFLFGA 182 Query: 96 LLGLW 100 + G+W Sbjct: 183 MQGIW 187 >ref|ZP_03543542.1| AMP-dependent synthetase and ligase [Comamonas testosteroni KF-1] gb|EED67828.1| AMP-dependent synthetase and ligase [Comamonas testosteroni KF-1] Length = 503 Score = 182 bits (461), Expect = 6e-44, Method: Compositional matrix adjust. Identities = 88/154 (57%), Positives = 115/154 (74%), Gaps = 5/154 (3%) Query: 113 GLIYVRSPMVFTDYVTLRDSDEQPALLRDGDWLSVRDVGHLDKQGRLHLLGRQQRMLLAQ 172 GLIY+RSPM+F DYV D+ + A+LRDGDWLSVRD+GH+D++G L L GRQ RM++ Q Sbjct: 327 GLIYIRSPMLFMDYVG--DAHDATAVLRDGDWLSVRDMGHIDERGMLCLAGRQSRMIVTQ 384 Query: 173 GKNLFPEEVESVLTAHPAVAAASVQGMADAVRGVQIAALLK---VTDPVSPEALVAWCRE 229 GKNLFPEEVE++L +HPA+A S+ G ADA+RG+Q+ A+L+ T+ S L W RE Sbjct: 385 GKNLFPEEVENLLASHPAIAQVSLHGQADALRGLQVHAVLQWRHQTEAPSALELNQWLRE 444 Query: 230 RLEPYKIPRRFYTCNDWPLTPSGKTDHKQLAQRL 263 R E +K+PR+++ C WP T SGKTDH QLAQ L Sbjct: 445 RTEAFKVPRQWWICEHWPQTASGKTDHGQLAQAL 478 Score = 89.0 bits (219), Expect = 6e-16, Method: Compositional matrix adjust. Identities = 41/76 (53%), Positives = 50/76 (65%) Query: 25 GAETALAKTTSSSPFYIGFTSGSSGLPKGFRRSHGSWTSSFDICLESFGAGAAATLLVPG 84 G L ++ FY GFTSGS+GLPKGF+R H SWT SF + L+ FG A L PG Sbjct: 112 GQPCELESAAPTAAFYTGFTSGSTGLPKGFKRHHLSWTESFRVGLQDFGPVVAQRTLAPG 171 Query: 85 RLSHSTFLFGALLGLW 100 R+SHS FLFGA+ G+W Sbjct: 172 RISHSLFLFGAMQGIW 187 >ref|ZP_08405135.1| AMP-dependent synthetase/ligase [Hylemonella gracilis ATCC 19624] gb|EGI77756.1| AMP-dependent synthetase/ligase [Hylemonella gracilis ATCC 19624] Length = 467 Score = 180 bits (457), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 86/158 (54%), Positives = 116/158 (73%), Gaps = 5/158 (3%) Query: 113 GLIYVRSPMVFTDYVTLRDSDEQPALLRDGDWLSVRDVGHLDKQGRLHLLGRQQRMLLAQ 172 G I+VRSPM+F DYV + + A +RDGDWLSVRD+G++D QG L LLGR+ RM++ Q Sbjct: 306 GQIFVRSPMLFMDYVG--PNPDHTAAVRDGDWLSVRDMGYIDAQGYLCLLGRENRMVVTQ 363 Query: 173 GKNLFPEEVESVLTAHPAVAAASVQGMADAVRGVQIAALLKVTDP---VSPEALVAWCRE 229 GKNLFPEE+E+VL A P +A ASVQG+ DA+RG Q+ A+L+ D ++ + + A CRE Sbjct: 364 GKNLFPEEIEAVLLADPRIAHASVQGVTDALRGQQVVAILQFKDAANTLTAQNVAALCRE 423 Query: 230 RLEPYKIPRRFYTCNDWPLTPSGKTDHKQLAQRLDDPA 267 LE YK+P+R + C+ WPLT SGKTDH LA+ L +P+ Sbjct: 424 HLEAYKVPKRLWVCDHWPLTTSGKTDHAALAKALQNPS 461 Score = 94.0 bits (232), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 41/71 (57%), Positives = 50/71 (70%) Query: 30 LAKTTSSSPFYIGFTSGSSGLPKGFRRSHGSWTSSFDICLESFGAGAAATLLVPGRLSHS 89 +A T +PFYIGFTSGS+G PKGFRR H SWT S C ++FG + ++ PGR SHS Sbjct: 109 MAMPTPRTPFYIGFTSGSTGSPKGFRRHHQSWTESLLACQDTFGEDVSRRVMAPGRFSHS 168 Query: 90 TFLFGALLGLW 100 FLFG +LGLW Sbjct: 169 LFLFGMILGLW 179 >ref|YP_002553002.1| AMP-dependent synthetase and ligase [Acidovorax ebreus TPSY] gb|ACM33002.1| AMP-dependent synthetase and ligase [Acidovorax ebreus TPSY] Length = 494 Score = 180 bits (457), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 91/163 (55%), Positives = 114/163 (69%), Gaps = 7/163 (4%) Query: 111 IPGLIYVRSPMVFTDYVTLRDSDEQPALLRDGDWLSVRDVGHLDKQGRLHLLGRQQRMLL 170 +PGLI+VRSPM+F DYV D A LR+GDWLSVRD+G++D G LHL GR+ RML+ Sbjct: 330 VPGLIWVRSPMLFMDYVNAADGT---AALREGDWLSVRDMGYVDDSGLLHLCGRENRMLV 386 Query: 171 AQGKNLFPEEVESVLTAHPAVAAASVQGMADAVRGVQIAALLKVTDPVSPEA---LVAWC 227 QGKNLFPEEVE+ L AHP VA AS+QG+AD +RG+++ A+L+ P L AWC Sbjct: 387 TQGKNLFPEEVEARLLAHPQVAQASLQGVADGLRGLRVQAVLQWQGDAPPPGALELAAWC 446 Query: 228 RERLEPYKIPRRFYT-CNDWPLTPSGKTDHKQLAQRLDDPAMP 269 R LEPYK PR ++ DWP TPSGKTDH +A+ L P+ P Sbjct: 447 RAVLEPYKAPRHWWVWVGDWPQTPSGKTDHTAIARALQAPSPP 489 Score = 84.0 bits (206), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 44/85 (51%), Positives = 53/85 (62%), Gaps = 5/85 (5%) Query: 5 HWRSGVGAETALAXRVAAGVGAETALAKTTSSSPFYIGFTSGSSGLPKGFRRSHGSWTSS 64 W +GV A+A R+ G G A T SPFY+GFTSGS+GLPKGF R H SW S Sbjct: 110 QWPAGVA--RAVAERLPLGSGGPAA---ATPQSPFYVGFTSGSTGLPKGFMRHHRSWVES 164 Query: 65 FDICLESFGAGAAATLLVPGRLSHS 89 F + L+ FG AA +L PGR+SHS Sbjct: 165 FRVSLQDFGVAAATRVLAPGRMSHS 189