BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0203.p (354 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_004154639.1| 2-nitropropane dioxygenase npd [Variovorax p... 416 e-114 ref|ZP_01892413.1| 2-nitropropane dioxygenase, NPD [Marinobacter... 405 e-111 ref|YP_982997.1| 2-nitropropane dioxygenase [Polaromonas naphtha... 398 e-109 ref|YP_002945189.1| 2-nitropropane dioxygenase NPD [Variovorax p... 397 e-108 ref|YP_004378423.1| 2-nitropropane dioxygenase, NPD [Pseudomonas... 389 e-106 >ref|YP_004154639.1| 2-nitropropane dioxygenase npd [Variovorax paradoxus EPS] gb|ADU36528.1| 2-nitropropane dioxygenase NPD [Variovorax paradoxus EPS] Length = 351 Score = 416 bits (1069), Expect = e-114, Method: Compositional matrix adjust. Identities = 228/322 (70%), Positives = 252/322 (78%), Gaps = 15/322 (4%) Query: 1 MEILQTLLGIELPLIQAPLAGIQGHAMAIAVSNAGGLGSLPCAMLTPETLRKELELIRAG 60 M LQ +LG ELPLIQAP+AGIQ AMAIAVSNAGGLGSLPCAML+PE +R EL IRAG Sbjct: 1 MTTLQKILGTELPLIQAPMAGIQVSAMAIAVSNAGGLGSLPCAMLSPEVMRSELAAIRAG 60 Query: 61 TSKPYNVNFFAHTPPTPDAAREX---------------DPDTIPQGAGRAPFSAEAAELL 105 T KPYNVNFF HTPP P RE D +IP G GR PFSA+ A+LL Sbjct: 61 TDKPYNVNFFCHTPPVPSTEREATWRAALAPYYAEYGIDATSIPTGPGRNPFSADVADLL 120 Query: 106 SEFKPAVVSFHFGLPDQALLDRVRSWGAKVLCSATTVPEARWLEEQGVDAIIAQGIEAGG 165 +EFKP VVSFHFGLP LL RV WGAKVL SATTV EA WLE G DA+IAQG+EAGG Sbjct: 121 AEFKPPVVSFHFGLPPAELLARVHGWGAKVLASATTVEEALWLEAHGADAVIAQGLEAGG 180 Query: 166 HRGFFLTHDLSTQIGTFALLPQIVRAVNVPVIAAGGIADAAGVAAAKSLGAAGVQIGTSY 225 HRG FL+HDL+ Q+GTFALLPQ+VRA+ VPVIAAGGIADA GVAAA +LGAAGVQIGT+Y Sbjct: 181 HRGHFLSHDLTKQLGTFALLPQLVRALKVPVIAAGGIADANGVAAAMALGAAGVQIGTAY 240 Query: 226 MLTPEASTSAPHRAALKGPAAEHTALTTLFTGGPARGIVNRVMRELGPLSSAAQVFPLAT 285 MLTPEA+TSA HRAALK AA HTALT LFTG PARGIVNRVMRELGP+ +AA FPLAT Sbjct: 241 MLTPEATTSAIHRAALKSEAARHTALTNLFTGRPARGIVNRVMRELGPIGAAAPEFPLAT 300 Query: 286 AAIAPLRSKAEAQGRGFFXVIF 307 + IAPLR+KAEAQG G F ++ Sbjct: 301 SGIAPLRAKAEAQGSGDFSPLW 322 >ref|ZP_01892413.1| 2-nitropropane dioxygenase, NPD [Marinobacter algicola DG893] gb|EDM49792.1| 2-nitropropane dioxygenase, NPD [Marinobacter algicola DG893] Length = 346 Score = 405 bits (1041), Expect = e-111, Method: Compositional matrix adjust. Identities = 209/319 (65%), Positives = 250/319 (78%), Gaps = 15/319 (4%) Query: 4 LQTLLGIELPLIQAPLAGIQGHAMAIAVSNAGGLGSLPCAMLTPETLRKELELIRAGTSK 63 +Q LLGIELP+IQAP+ G+QGHA+A+AVSNAGGLGSLPCAML+ E LRKELE + + TSK Sbjct: 3 IQNLLGIELPVIQAPMVGVQGHALAVAVSNAGGLGSLPCAMLSSEALRKELEALTSQTSK 62 Query: 64 PYNVNFFAHTPPTPDAARE---------------XDPDTIPQGAGRAPFSAEAAELLSEF 108 PYNVNFF HTPP PDA E D + + AGRAPFS+EAA++L++F Sbjct: 63 PYNVNFFCHTPPEPDADAERHWREALSPYFDELGIDSNNVSAQAGRAPFSSEAADVLADF 122 Query: 109 KPAVVSFHFGLPDQALLDRVRSWGAKVLCSATTVPEARWLEEQGVDAIIAQGIEAGGHRG 168 KP VVSFHFGLP L+ R+R WG K+L +ATTV EA WLE QGVDAIIAQG+EAGGHRG Sbjct: 123 KPPVVSFHFGLPAPELMARIRGWGGKILSTATTVDEALWLEAQGVDAIIAQGLEAGGHRG 182 Query: 169 FFLTHDLSTQIGTFALLPQIVRAVNVPVIAAGGIADAAGVAAAKSLGAAGVQIGTSYMLT 228 FFL+ D+S Q+GTFAL+PQ+V AV +PVIAAGGIAD+ GV AA +LGAAG QIGT+Y+L Sbjct: 183 FFLSRDISAQVGTFALIPQMVNAVKIPVIAAGGIADSRGVQAAMALGAAGAQIGTAYLLC 242 Query: 229 PEASTSAPHRAALKGPAAEHTALTTLFTGGPARGIVNRVMRELGPLSSAAQVFPLATAAI 288 PEA T A HRAALK A+ TALT LF+GG ARGIVNR+MRELGP+SS FPLA++AI Sbjct: 243 PEAQTKAVHRAALKSEASRVTALTNLFSGGAARGIVNRIMRELGPMSSKVPAFPLASSAI 302 Query: 289 APLRSKAEAQGRGFFXVIF 307 APLR+KAEA+G G F ++ Sbjct: 303 APLRAKAEAEGSGDFSPLW 321 >ref|YP_982997.1| 2-nitropropane dioxygenase [Polaromonas naphthalenivorans CJ2] gb|ABM38076.1| 2-nitropropane dioxygenase, NPD [Polaromonas naphthalenivorans CJ2] Length = 344 Score = 398 bits (1022), Expect = e-109, Method: Compositional matrix adjust. Identities = 209/319 (65%), Positives = 243/319 (76%), Gaps = 15/319 (4%) Query: 4 LQTLLGIELPLIQAPLAGIQGHAMAIAVSNAGGLGSLPCAMLTPETLRKELELIRAGTSK 63 +Q GIELP+IQAP+AG+Q A+AIAVSNAGGLGSLPCAMLTP+ LR EL I A T++ Sbjct: 3 IQNFFGIELPVIQAPMAGVQTSALAIAVSNAGGLGSLPCAMLTPDALRAELAAISAQTNR 62 Query: 64 PYNVNFFAHTPPTPDAAREX---------------DPDTIPQGAGRAPFSAEAAELLSEF 108 PYNVNFF HTP PD ARE DP T+P G GRAPFS+E A++L+ F Sbjct: 63 PYNVNFFCHTPAAPDTAREAKWRAALAPYYAEFGIDPATVPAGGGRAPFSSEFADVLAAF 122 Query: 109 KPAVVSFHFGLPDQALLDRVRSWGAKVLCSATTVPEARWLEEQGVDAIIAQGIEAGGHRG 168 +P VVSFHFGLP LL RV+SWGAKVL SATTV EARWLE G DAIIAQG+EAGGHRG Sbjct: 123 RPPVVSFHFGLPSPELLQRVKSWGAKVLSSATTVAEARWLEANGADAIIAQGLEAGGHRG 182 Query: 169 FFLTHDLSTQIGTFALLPQIVRAVNVPVIAAGGIADAAGVAAAKSLGAAGVQIGTSYMLT 228 FL+ DL+TQ+GT ALLPQ++RAV VPVIAAGGIADA GV AA +LGAAGVQ+GTSY+L Sbjct: 183 IFLSDDLTTQVGTMALLPQVLRAVRVPVIAAGGIADARGVTAALALGAAGVQVGTSYLLC 242 Query: 229 PEASTSAPHRAALKGPAAEHTALTTLFTGGPARGIVNRVMRELGPLSSAAQVFPLATAAI 288 PEA+T+A HRAALK AEHTALT L TG PARGI NR+M ELGP+S A FP AT+A+ Sbjct: 243 PEATTTALHRAALKSERAEHTALTNLITGRPARGIYNRLMSELGPVSDAPPAFPFATSAL 302 Query: 289 APLRSKAEAQGRGFFXVIF 307 PL++ AE GR F ++ Sbjct: 303 MPLKTAAEKLGRDDFSTLW 321 >ref|YP_002945189.1| 2-nitropropane dioxygenase NPD [Variovorax paradoxus S110] gb|ACS19923.1| 2-nitropropane dioxygenase NPD [Variovorax paradoxus S110] Length = 352 Score = 397 bits (1021), Expect = e-108, Method: Compositional matrix adjust. Identities = 224/319 (70%), Positives = 256/319 (80%), Gaps = 15/319 (4%) Query: 4 LQTLLGIELPLIQAPLAGIQGHAMAIAVSNAGGLGSLPCAMLTPETLRKELELIRAGTSK 63 LQ LLG +LPLIQAP+AG+QG AMAIAVSNAGGLGSLPCAML P+T+R EL IRAGT K Sbjct: 5 LQQLLGTDLPLIQAPMAGVQGSAMAIAVSNAGGLGSLPCAMLGPDTMRSELAAIRAGTGK 64 Query: 64 PYNVNFFAHTPPTPDAAREX---------------DPDTIPQGAGRAPFSAEAAELLSEF 108 P+NVNFF HTPP P A RE D +IP G GR PFSA+ A +L+EF Sbjct: 65 PFNVNFFCHTPPEPSAEREAAWRSALAPYYTEFGIDAASIPTGPGRNPFSADVATVLAEF 124 Query: 109 KPAVVSFHFGLPDQALLDRVRSWGAKVLCSATTVPEARWLEEQGVDAIIAQGIEAGGHRG 168 +P VVSFHFGLP +AL+ +V WGAKVL SATTV EARWLE +GVDA+IAQG+EAGGHRG Sbjct: 125 RPEVVSFHFGLPSEALMAQVHGWGAKVLASATTVEEARWLEARGVDAVIAQGLEAGGHRG 184 Query: 169 FFLTHDLSTQIGTFALLPQIVRAVNVPVIAAGGIADAAGVAAAKSLGAAGVQIGTSYMLT 228 FL+HDL+ Q+GTFALLPQ+V AV VPVIAAGGIADAAGVAAA +LGAAGVQ+GT+Y+L Sbjct: 185 HFLSHDLTRQLGTFALLPQLVHAVKVPVIAAGGIADAAGVAAAMALGAAGVQVGTAYLLA 244 Query: 229 PEASTSAPHRAALKGPAAEHTALTTLFTGGPARGIVNRVMRELGPLSSAAQVFPLATAAI 288 PEA+TSA HRAALK AA HTALT LFTG PARGIVNRVMRELGP+ +AA FPLAT+ I Sbjct: 245 PEATTSAVHRAALKSQAARHTALTNLFTGRPARGIVNRVMRELGPIGAAAPEFPLATSGI 304 Query: 289 APLRSKAEAQGRGFFXVIF 307 APLR+KAEAQG G F ++ Sbjct: 305 APLRAKAEAQGSGDFSPLW 323 >ref|YP_004378423.1| 2-nitropropane dioxygenase, NPD [Pseudomonas mendocina NK-01] gb|AEB56671.1| 2-nitropropane dioxygenase, NPD [Pseudomonas mendocina NK-01] Length = 348 Score = 389 bits (1000), Expect = e-106, Method: Compositional matrix adjust. Identities = 214/319 (67%), Positives = 249/319 (78%), Gaps = 15/319 (4%) Query: 4 LQTLLGIELPLIQAPLAGIQGHAMAIAVSNAGGLGSLPCAMLTPETLRKELELIRAGTSK 63 LQ LLG ELPLIQAP+AG QGH +A AV AGGLGS+PCAMLTP LR+ELE + T+ Sbjct: 3 LQRLLGSELPLIQAPMAGSQGHRLAAAVCQAGGLGSIPCAMLTPAALRQELEAMLGLTAH 62 Query: 64 PYNVNFFAHTPPTPDAAREX---------------DPDTIPQGAGRAPFSAEAAELLSEF 108 P+N+NFF+H PP PDAARE D I G GR PF+ EAA L+ EF Sbjct: 63 PFNLNFFSHLPPEPDAAREALWREMLAPYYQELGVDQANIANGPGRLPFNQEAAALVEEF 122 Query: 109 KPAVVSFHFGLPDQALLDRVRSWGAKVLCSATTVPEARWLEEQGVDAIIAQGIEAGGHRG 168 +PAVVSFHFGLPD+ LL RVR GAK+L SATT+ EA WL+E+GVDA+IAQG+EAGGHRG Sbjct: 123 RPAVVSFHFGLPDETLLQRVRRSGAKILSSATTLDEALWLQERGVDAVIAQGLEAGGHRG 182 Query: 169 FFLTHDLSTQIGTFALLPQIVRAVNVPVIAAGGIADAAGVAAAKSLGAAGVQIGTSYMLT 228 FL HDLS Q+G FALLPQ+V A++VPVIAAGGI+DA GVAAA +LGAAGVQIG++Y+L Sbjct: 183 HFLDHDLSRQLGIFALLPQLVDALSVPVIAAGGISDARGVAAAMALGAAGVQIGSAYLLC 242 Query: 229 PEASTSAPHRAALKGPAAEHTALTTLFTGGPARGIVNRVMRELGPLSSAAQVFPLATAAI 288 PEA+TSA HRAAL+ PAA HTALT LF+G PARGIVNR+MRELGPLSS A FPLATAAI Sbjct: 243 PEANTSAIHRAALQSPAARHTALTNLFSGRPARGIVNRLMRELGPLSSQAPAFPLATAAI 302 Query: 289 APLRSKAEAQGRGFFXVIF 307 APLR+ AEAQG G F ++ Sbjct: 303 APLRTAAEAQGNGDFSPLW 321